{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:49:11Z","timestamp":1760147351570,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,27]],"date-time":"2023-01-27T00:00:00Z","timestamp":1674777600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objectives: Coronary artery disease (CAD) is the foremost cause of adult disability and mortality. There is an urgent need to focus on the research of new approaches for the prevention and treatment of CAD. Materials and Methods: The effects of peptides isolated from the blood plasma of CAD patients on endothelial cell secretion using the in vitro model have been tested. Human endothelial progenitor cells (HEPCs) were incubated for 24 h with peptides isolated from the plasma of healthy subjects or patients with stable angina, progressive unstable angina, and myocardial infarction. The contents of some soluble anticoagulant as well as procoagulant mediators in HEPC culture treated with peptide pools were then compared. Results and Conclusion: The results show that peptides from the plasma of patients with myocardial infarction promote endothelial cells to release both von Willebrand factor and endothelin-1, increasing vasoconstriction and shifting hemostatic balance toward a prothrombotic state. In contrast, peptides from the plasma of patients with progressive unstable angina suppress the secretion of endothelin-1 by HEPCs, while the secretion of both von Willebrand factor and tissue plasminogen activator was increased. As can be seen from the results obtained, disease derived peptides may contribute to the homeostasis of living organisms or the progression of pathological processes.<\/jats:p>","DOI":"10.3390\/medicina59020238","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T03:56:27Z","timestamp":1675050987000},"page":"238","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Effect of Peptides from Plasma of Patients with Coronary Artery Disease on the Vascular Endothelial Cells"],"prefix":"10.3390","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4062-8911","authenticated-orcid":false,"given":"Marko","family":"Kozyk","sequence":"first","affiliation":[{"name":"Corewell Health William Beaumont University Hospital, Royal Oak, MI 48073, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8330-1180","authenticated-orcid":false,"given":"Kateryna","family":"Strubchevska","sequence":"additional","affiliation":[{"name":"Corewell Health William Beaumont University Hospital, Royal Oak, MI 48073, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetiana","family":"Marynenko","sequence":"additional","affiliation":[{"name":"Educational and Scientific Centre \u201cInstitute of Biology and Medicine\u201d, Taras Shevchenko National University of Kyiv, 64\/13 Volodymyrska Str., 01601 Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alena","family":"Zlatska","sequence":"additional","affiliation":[{"name":"State Institute of Genetic and Regenerative Medicine NAMS of Ukraine, 67 Vyshgorodska Str., 04114 Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetiana","family":"Halenova","sequence":"additional","affiliation":[{"name":"Educational and Scientific Centre \u201cInstitute of Biology and Medicine\u201d, Taras Shevchenko National University of Kyiv, 64\/13 Volodymyrska Str., 01601 Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nataliia","family":"Raksha","sequence":"additional","affiliation":[{"name":"Educational and Scientific Centre \u201cInstitute of Biology and Medicine\u201d, Taras Shevchenko National University of Kyiv, 64\/13 Volodymyrska Str., 01601 Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3621-6981","authenticated-orcid":false,"given":"Olexii","family":"Savchuk","sequence":"additional","affiliation":[{"name":"Educational and Scientific Centre \u201cInstitute of Biology and Medicine\u201d, Taras Shevchenko National University of Kyiv, 64\/13 Volodymyrska Str., 01601 Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetyana","family":"Falalyeyeva","sequence":"additional","affiliation":[{"name":"Educational and Scientific Centre \u201cInstitute of Biology and Medicine\u201d, Taras Shevchenko National University of Kyiv, 64\/13 Volodymyrska Str., 01601 Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6311-3518","authenticated-orcid":false,"given":"Oleksandr","family":"Kovalchuk","sequence":"additional","affiliation":[{"name":"Educational and Scientific Centre \u201cInstitute of Biology and Medicine\u201d, Taras Shevchenko National University of Kyiv, 64\/13 Volodymyrska Str., 01601 Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ludmyla","family":"Ostapchenko","sequence":"additional","affiliation":[{"name":"Educational and Scientific Centre \u201cInstitute of Biology and Medicine\u201d, Taras Shevchenko National University of Kyiv, 64\/13 Volodymyrska Str., 01601 Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"16812","DOI":"10.1002\/jcp.28350","article-title":"A review on coronary artery disease, its risk factors, and therapeutics","volume":"234","author":"Malakar","year":"2019","journal-title":"J. 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