{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:55:13Z","timestamp":1787028913838,"version":"build-2736575974"},"reference-count":67,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T00:00:00Z","timestamp":1686096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education, Government of Bangladesh","award":["37.20.0000.004.33.006.2020-3782"],"award-info":[{"award-number":["37.20.0000.004.33.006.2020-3782"]}]},{"name":"Ministry of Education, Government of Bangladesh","award":["PNURSP2023R342"],"award-info":[{"award-number":["PNURSP2023R342"]}]},{"name":"Princess Nourah bint Abdulrahman University","award":["37.20.0000.004.33.006.2020-3782"],"award-info":[{"award-number":["37.20.0000.004.33.006.2020-3782"]}]},{"name":"Princess Nourah bint Abdulrahman University","award":["PNURSP2023R342"],"award-info":[{"award-number":["PNURSP2023R342"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Nucleoside analogs are frequently used in the control of viral infections and neoplastic diseases. However, relatively few studies have shown that nucleoside analogs have antibacterial and antifungal activities. In this study, a fused pyrimidine molecule, uridine, was modified with various aliphatic chains and aromatic groups to produce new derivatives as antimicrobial agents. All newly synthesized uridine derivatives were analyzed by spectral (NMR, FTIR, mass spectrometry), elemental, and physicochemical analyses. Prediction of activity spectra for substances (PASS) and in vitro biological evaluation against bacteria and fungi indicated promising antimicrobial capability of these uridine derivatives. The tested compounds were more effective against fungal phytopathogens than bacterial strains, as determined by their in vitro antimicrobial activity. Cytotoxicity testing indicated that the compounds were less toxic. In addition, antiproliferative activity against Ehrlich ascites carcinoma (EAC) cells was investigated, and compound 6 (2\u2032,3\u2032-di-O-cinnamoyl-5\u2032-O-palmitoyluridine) demonstrated promising anticancer activity. Their molecular docking against Escherichia coli (1RXF) and Salmonella typhi (3000) revealed notable binding affinities and nonbonding interactions in support of this finding. Stable conformation and binding patterns\/energy were found in a stimulating 400 ns molecular dynamics (MD) simulation. Structure\u2013activity relationship (SAR) investigation indicated that acyl chains, CH3(CH2)10CO-, (C6H5)3C-, and C2H5C6H4CO-, combined with deoxyribose, were most effective against the tested bacterial and fungal pathogens. Pharmacokinetic predictions were examined to determine their ADMET characteristics, and the results in silico were intriguing. Finally, the synthesized uridine derivatives demonstrated increased medicinal activity and high potential for future antimicrobial\/anticancer agent(s).<\/jats:p>","DOI":"10.3390\/medicina59061107","type":"journal-article","created":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T01:34:32Z","timestamp":1686188072000},"page":"1107","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Uridine Derivatives: Synthesis, Biological Evaluation, and In Silico Studies as Antimicrobial and Anticancer Agents"],"prefix":"10.3390","volume":"59","author":[{"given":"Nasrin S.","family":"Munia","sequence":"first","affiliation":[{"name":"Laboratory of Carbohydrate and Nucleoside Chemistry (LCNC), Department of Chemistry, Faculty of Science, University of Chittagong, Chittagong 4331, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0483-8113","authenticated-orcid":false,"given":"Mohammed M.","family":"Alanazi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5759-6568","authenticated-orcid":false,"given":"Youness","family":"El Bakri","sequence":"additional","affiliation":[{"name":"Department of Theoretical and Applied Chemistry, South Ural State University, Lenin prospect 76, Chelyabinsk 454080, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1042-8126","authenticated-orcid":false,"given":"Ashwag S.","family":"Alanazi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6268-0775","authenticated-orcid":false,"given":"Yousef E.","family":"Mukhrish","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Jazan University, P.O. Box 2097, Jazan 45142, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4715-9022","authenticated-orcid":false,"given":"Imtiaj","family":"Hasan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Faculty of Science, University of Rajshahi, Rajshahi 6205, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7964-9117","authenticated-orcid":false,"given":"Sarkar M. A.","family":"Kawsar","sequence":"additional","affiliation":[{"name":"Laboratory of Carbohydrate and Nucleoside Chemistry (LCNC), Department of Chemistry, Faculty of Science, University of Chittagong, Chittagong 4331, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.3762\/bjoc.10.179","article-title":"Multicomponent Reactions in Nucleoside Chemistry","volume":"10","author":"Buchowicz","year":"2014","journal-title":"Beilstein J. Org. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/j.ijantimicag.2012.01.019","article-title":"Gemcitabine is Active against Clinical Multiresistant Staphylococcus aureus Strains and is Synergistic with Gentamicin","volume":"39","author":"Jordheim","year":"2012","journal-title":"Int. J. Antimicrob. 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