{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T13:45:18Z","timestamp":1783518318557,"version":"3.55.0"},"reference-count":42,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,10]],"date-time":"2023-08-10T00:00:00Z","timestamp":1691625600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Anhui Institute of Translational Medicine","award":["2022zhyx-C86"],"award-info":[{"award-number":["2022zhyx-C86"]}]},{"name":"Anhui Institute of Translational Medicine","award":["2022xkfyhz01"],"award-info":[{"award-number":["2022xkfyhz01"]}]},{"name":"2022 Disciplinary Construction Project in the School of Dentistry, Anhui Medical University","award":["2022zhyx-C86"],"award-info":[{"award-number":["2022zhyx-C86"]}]},{"name":"2022 Disciplinary Construction Project in the School of Dentistry, Anhui Medical University","award":["2022xkfyhz01"],"award-info":[{"award-number":["2022xkfyhz01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background: Oral lichen planus (OLP) is an infrequent autoimmune disease of the oral mucosa, which affects up to 2% of the world population. An investigation of Tripterygium wilfordii\u2019s mechanism of action for treating OLP was conducted, and a theoretical basis was provided for improving current treatment regimens. Materials and Methods: We used a network pharmacological approach to gain insight into the molecular mechanism of Tripterygium wilfordii in the treatment of OLP. Then, potential protein targets between Tripterygium wilfordii and OLP were analyzed through a drug\u2013target network. This was followed by KEGG enrichment analysis and Gene Ontology (GO) classification. Finally, for molecular docking, AutoDock Vina was used. Results: A protein\u2013protein interaction (PPI) network was constructed by analyzing the common targets of a total of 51 wilfordii\u2013OLP interactions from different databases. The GO and KEGG enrichment analyses showed that the treatment of OLP with Tripterygium wilfordii mainly involves lipopolysaccharide response, bacterial molecular response, positive regulation of cytokine production, and leukocyte proliferation, and the signaling pathways mainly include the AGE-RAGE, NF-\u03baB, Toll-like receptor, IL-17, HIF-1, and TNF signaling pathways. The molecular docking results showed that \u03b2-sitosterol, kaempferol, hederagenin, and triptolide have a higher affinity for AKT1, TNF, CASP3, and PTGS2, respectively. Based on the CytoNCA analysis of common targets, 19 key targets, including AKT1, TNF, VEGFA, STAT3, CXCL8, PTGS2, TP53, and CASP3, and their connections were identified. Conclusions: Preliminarily, this study reveals that Tripterygium wilfordii interferes with OLP by interacting with multiple targets through multiple accesses, as validated by molecular docking.<\/jats:p>","DOI":"10.3390\/medicina59081448","type":"journal-article","created":{"date-parts":[[2023,8,10]],"date-time":"2023-08-10T10:11:22Z","timestamp":1691662282000},"page":"1448","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Network Pharmacology and Molecular Docking Analysis Exploring the Mechanism of Tripterygium wilfordii in the Treatment of Oral Lichen Planus"],"prefix":"10.3390","volume":"59","author":[{"given":"Wenkai","family":"Huang","sequence":"first","affiliation":[{"name":"College & Hospital of Stomatology, Key Lab. of Oral Diseases Research of Anhui Province, Anhui Medical University, No. 81, Meishan Road, Shushan District, Hefei 230032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Huang","sequence":"additional","affiliation":[{"name":"College & Hospital of Stomatology, Key Lab. of Oral Diseases Research of Anhui Province, Anhui Medical University, No. 81, Meishan Road, Shushan District, Hefei 230032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Yang","sequence":"additional","affiliation":[{"name":"College & Hospital of Stomatology, Key Lab. of Oral Diseases Research of Anhui Province, Anhui Medical University, No. 81, Meishan Road, Shushan District, Hefei 230032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjia","family":"Han","sequence":"additional","affiliation":[{"name":"College & Hospital of Stomatology, Key Lab. of Oral Diseases Research of Anhui Province, Anhui Medical University, No. 81, Meishan Road, Shushan District, Hefei 230032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongqing","family":"Zhu","sequence":"additional","affiliation":[{"name":"College & Hospital of Stomatology, Key Lab. of Oral Diseases Research of Anhui Province, Anhui Medical University, No. 81, Meishan Road, Shushan District, Hefei 230032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyin","family":"Wang","sequence":"additional","affiliation":[{"name":"College & Hospital of Stomatology, Key Lab. of Oral Diseases Research of Anhui Province, Anhui Medical University, No. 81, Meishan Road, Shushan District, Hefei 230032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0296-1724","authenticated-orcid":false,"given":"Ran","family":"Chen","sequence":"additional","affiliation":[{"name":"College & Hospital of Stomatology, Key Lab. of Oral Diseases Research of Anhui Province, Anhui Medical University, No. 81, Meishan Road, Shushan District, Hefei 230032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1959506","DOI":"10.1155\/2018\/1959506","article-title":"Markers of Oral Lichen Planus Malignant Transformation","volume":"2018","author":"Tampa","year":"2018","journal-title":"Dis. Markers"},{"key":"ref_2","first-page":"2256","article-title":"Efficacy of injectable platelet-rich fibrin in the treatment of symptomatic oral lichen planus","volume":"29","author":"Hamadah","year":"2022","journal-title":"Oral Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1002\/ptr.6236","article-title":"Herbal medicine in oral lichen planus","volume":"33","author":"Ghahremanlo","year":"2019","journal-title":"Phytother. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1111\/odi.14142","article-title":"Immune mechanisms in oral lichen planus","volume":"29","author":"Thornhill","year":"2023","journal-title":"Oral Dis."},{"key":"ref_5","first-page":"885","article-title":"Salivary oxidative status in patients with oral lichen planus","volume":"67","author":"Darczuk","year":"2016","journal-title":"J. Physiol. Pharmacol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"691031","DOI":"10.3389\/fphar.2021.691031","article-title":"Efficacy and Safety of Tripterygium Wilfordii Hook. F for Connective Tissue Disease-Associated Interstitial Lung Disease: A Systematic Review and Meta-Analysis","volume":"12","author":"Li","year":"2021","journal-title":"Front. Pharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"361865","DOI":"10.1155\/2020\/2361865","article-title":"Deciphering the Active Ingredients and Molecular Mechanisms of Tripterygium hypoglaucum (Levl.) Hutch against Rheumatoid Arthritis Based on Network Pharmacology","volume":"2020","author":"Jiang","year":"2020","journal-title":"Evid. Based Complement. Alternat. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5782922","DOI":"10.1155\/2022\/5782922","article-title":"Topical Treatment of Colquhounia Root Relieves Skin Inflammation and Itch in Imiquimod-Induced Psoriasiform Dermatitis in Mice","volume":"2022","author":"Li","year":"2022","journal-title":"Mediat. Inflamm."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s13020-021-00563-7","article-title":"Repurposing a clinically approved prescription Colquhounia root tablet to treat diabetic kidney disease via suppressing PI3K\/AKT\/NF-kB activation","volume":"17","author":"Ma","year":"2022","journal-title":"Chin. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0889-857X(05)70118-6","article-title":"The Chinese anti-inflammatory and immunosuppressive herbal remedy Tripterygium wilfordii Hook F","volume":"26","author":"Tao","year":"2000","journal-title":"Rheum. Dis. Clin. N. Am."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2180","DOI":"10.1002\/ptr.6672","article-title":"Efficacy and safety of Tripterygium wilfordii Hook. f. for oral lichen planus: Evidence from 18 randomized controlled trials","volume":"34","author":"Luo","year":"2020","journal-title":"Phytother. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1038\/nbt1007-1110","article-title":"Network pharmacology","volume":"25","author":"Hopkins","year":"2007","journal-title":"Nat. Biotechnol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"590994","DOI":"10.3389\/fphar.2021.590994","article-title":"Qing-Yi Decoction in the Treatment of Acute Pancreatitis: An Integrated Approach Based on Chemical Profile, Network Pharmacology, Molecular Docking and Experimental Evaluation","volume":"12","author":"Wei","year":"2021","journal-title":"Front. Pharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/1758-2946-6-13","article-title":"TCMSP: A database of systems pharmacology for drug discovery from herbal medicines","volume":"6","author":"Ru","year":"2014","journal-title":"J. Cheminform."},{"key":"ref_15","first-page":"4599305","article-title":"Identification of Biomarkers Associated with Cancerous Change in Oral Leukoplakia Based on Integrated Transcriptome Analysis","volume":"2022","author":"Li","year":"2022","journal-title":"J. Oncol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4441","DOI":"10.1007\/s00784-019-02892-2","article-title":"Severity of oral lichen planus and oral lichenoid lesions is associated with anxiety","volume":"23","author":"Zucoloto","year":"2019","journal-title":"Clin. Oral Investig."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2384","DOI":"10.1111\/jdv.16324","article-title":"Topical rapamycin versus betamethasone dipropionate ointment for treating oral erosive lichen planus: A randomized, double-blind, controlled study","volume":"34","author":"Samimi","year":"2020","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_18","first-page":"92","article-title":"Comparison of the Effects of Curcumin Mucoadhesive Paste and Local Corticosteroid on the Treatment of Erosive Oral Lichen Planus Lesions","volume":"110","author":"Nosratzehi","year":"2018","journal-title":"J. Natl. Med. Assoc."},{"key":"ref_19","first-page":"793","article-title":"Randomized trial of aloe vera gel vs. triamcinolone acetonide ointment in the treatment of oral lichen planus","volume":"43","author":"Reddy","year":"2012","journal-title":"Quintessence Int."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.jep.2014.06.006","article-title":"Triptolide: Progress on research in pharmacodynamics and toxicology","volume":"155","author":"Li","year":"2014","journal-title":"J. Ethnopharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Zhou, W., Wang, Y., Lu, A., and Zhang, G. (2016). Systems Pharmacology in Small Molecular Drug Discovery. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17020246"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"112055","DOI":"10.1016\/j.jep.2019.112055","article-title":"A network pharmacology integrated pharmacokinetics strategy for uncovering pharmacological mechanism of compounds absorbed into the blood of Dan-Lou tablet on coronary heart disease","volume":"242","author":"Ding","year":"2019","journal-title":"J. Ethnopharmacol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"6429","DOI":"10.1158\/0008-5472.CAN-17-1662","article-title":"Multiscale Modeling of Inflammation-Induced Tumorigenesis Reveals Competing Oncogenic and Oncoprotective Roles for Inflammation","volume":"77","author":"Guo","year":"2017","journal-title":"Cancer Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.3389\/fphar.2020.01118","article-title":"Kaempferol Protects Blood Vessels From Damage Induced by Oxidative Stress and Inflammation in Association with the Nrf2\/HO-1 Signaling Pathway","volume":"11","author":"Yao","year":"2020","journal-title":"Front. Pharmacol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"110702","DOI":"10.1016\/j.biopha.2020.110702","article-title":"An update on beta-sitosterol: A potential herbal nutraceutical for diabetic management","volume":"131","author":"Babu","year":"2020","journal-title":"Biomed. Pharmacother."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1016\/j.bmcl.2018.03.073","article-title":"Beta-sitosterol and its derivatives repress lipopolysaccharide\/d-galactosamine-induced acute hepatic injury by inhibiting the oxidation and inflammation in mice","volume":"28","author":"Yin","year":"2018","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1038\/ki.2008.203","article-title":"Triptolide protects podocytes from puromycin aminonucleoside induced injury in vivo and in vitro","volume":"74","author":"Zheng","year":"2008","journal-title":"Kidney Int."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.7150\/ijbs.24032","article-title":"Triptolide Attenuates Renal Tubular Epithelial-mesenchymal Transition via the MiR-188-5p-mediated PI3K\/AKT Pathway in Diabetic Kidney Disease","volume":"14","author":"Xue","year":"2018","journal-title":"Int. J. Biol. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"110122","DOI":"10.1016\/j.intimp.2023.110122","article-title":"Hederagenin ameliorates renal fibrosis in chronic kidney disease through blocking ISG15 regulated JAK\/STAT signaling","volume":"118","author":"Jia","year":"2023","journal-title":"Int. Immunopharmacol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1007\/s10753-022-01763-5","article-title":"Hederagenin Suppresses Inflammation and Cartilage Degradation to Ameliorate the Progression of Osteoarthritis: An In vivo and In vitro Study","volume":"46","author":"Shen","year":"2023","journal-title":"Inflammation"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1016\/j.cellsig.2011.05.004","article-title":"Akt signalling in health and disease","volume":"23","author":"Hers","year":"2011","journal-title":"Cell. Signal."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/j.imbio.2019.01.004","article-title":"Aberrant IGF1-PI3K\/AKT\/MTOR signaling pathway regulates the local immunity of oral lichen planus","volume":"224","author":"Ma","year":"2019","journal-title":"Immunobiology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1111\/j.1365-2230.2006.02343.x","article-title":"Levamisole can reduce the high serum tumour necrosis factor-alpha level to a normal level in patients with erosive oral lichen planus","volume":"32","author":"Sun","year":"2007","journal-title":"Clin. Exp. Dermatol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.cyto.2007.11.004","article-title":"NF-kappaB-dependent cytokines in saliva and serum from patients with oral lichen planus: A study in an ethnic Chinese population","volume":"41","author":"Zhang","year":"2008","journal-title":"Cytokine"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.biopha.2018.05.076","article-title":"Total glucosides of paeony improves the immunomodulatory capacity of MSCs partially via the miR-124\/STAT3 pathway in oral lichen planus","volume":"105","author":"Zhao","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cytogfr.2016.05.001","article-title":"STAT3 signaling in immunity","volume":"31","author":"Hillmer","year":"2016","journal-title":"Cytokine Growth Factor Rev."},{"key":"ref_37","first-page":"1278","article-title":"Expression and role of p53 in oral lichen planus patients","volume":"22","author":"Petrovic","year":"2017","journal-title":"J. BUON"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"5958","DOI":"10.1158\/0008-5472.CAN-19-0840","article-title":"Cleaved Caspase-3 Transcriptionally Regulates Angiogenesis-Promoting Chemotherapy Resistance","volume":"79","author":"Bernard","year":"2019","journal-title":"Cancer Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"9914652","DOI":"10.1155\/2021\/9914652","article-title":"Systematic Review and Meta-Analysis of Oxidative Stress and Antioxidant Markers in Oral Lichen Planus","volume":"2021","author":"Wang","year":"2021","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.gene.2012.07.045","article-title":"The molecular mechanisms of the effect of Dexamethasone and Cyclosporin A on TLR4 \/NF-kappaB signaling pathway activation in oral lichen planus","volume":"508","author":"Ge","year":"2012","journal-title":"Gene"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1080\/08830185.2017.1380200","article-title":"Toll-like receptors: Significance, ligands, signaling pathways, and functions in mammals","volume":"37","author":"Vidya","year":"2018","journal-title":"Int. Rev. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"701094","DOI":"10.1155\/2014\/701094","article-title":"Overexpression and selectively regulatory roles of IL-23\/IL-17 axis in the lesions of oral lichen planus","volume":"2014","author":"Lu","year":"2014","journal-title":"Mediat. Inflamm."}],"container-title":["Medicina"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1648-9144\/59\/8\/1448\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:30:41Z","timestamp":1760128241000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1648-9144\/59\/8\/1448"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,10]]},"references-count":42,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["medicina59081448"],"URL":"https:\/\/doi.org\/10.3390\/medicina59081448","relation":{},"ISSN":["1648-9144"],"issn-type":[{"value":"1648-9144","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,10]]}}}