{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T21:18:14Z","timestamp":1785532694278,"version":"3.56.0"},"reference-count":35,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T00:00:00Z","timestamp":1695859200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Construction Fund of Key Medical Disciplines of Hangzhou","award":["OO20200121"],"award-info":[{"award-number":["OO20200121"]}]},{"name":"Construction Fund of Key Medical Disciplines of Hangzhou","award":["2022ZZ028"],"award-info":[{"award-number":["2022ZZ028"]}]},{"name":"Traditional Chinese Medicine Science and Technology Plan Project of Zhejiang Province","award":["OO20200121"],"award-info":[{"award-number":["OO20200121"]}]},{"name":"Traditional Chinese Medicine Science and Technology Plan Project of Zhejiang Province","award":["2022ZZ028"],"award-info":[{"award-number":["2022ZZ028"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objectives: With the growing incidence and disability associated with myocardial infarction (MI), there is an increasing focus on cardiac rehabilitation post-MI. Kuanxiongzhuyu decoction (KXZY), a traditional Chinese herbal formula, has been used in the rehabilitation of patients after MI. However, the chemical composition, protective effects, and underlying mechanism of KXZY remain unclear. Materials and Methods: In this study, the compounds in KXZY were identified using a high-performance liquid chromatography-mass spectrometry (HPLC-MS) analytical method. Based on the compounds identified in the KXZY, we predictively selected the potential targets of MI and then constructed a protein\u2013protein interaction (PPI) network to identify the key targets. Furthermore, the DAVID database was used for the GO and KEGG analyses, and molecular docking was used to verify the key targets. Finally, the cardioprotective effects and mechanism of KXZY were investigated in post-MI mice. Results: A total of 193 chemical compounds of KXZY were identified by HPLC-MS. In total, 228 potential targets were obtained by the prediction analysis. The functional enrichment studies and PPI network showed that the targets were largely associated with AKT-pathway-related apoptosis. The molecular docking verified that isoguanosine and adenosine exhibited excellent binding to the AKT. In vivo, KXZY significantly alleviated cardiac dysfunction and suppressed AKT phosphorylation. Furthermore, KXZY significantly increased the expression of the antiapoptotic proteins Bcl-2 and Bcl-xl and decreased the expression of the proapoptotic protein BAD. Conclusions: In conclusion, the network pharmacological and experimental evidence suggests that KXZY manifests anti-cardiac dysfunction behavior by alleviating cardiomyocyte apoptosis via the AKT pathway in MI and, thus, holds promising therapeutic potential.<\/jats:p>","DOI":"10.3390\/medicina59101740","type":"journal-article","created":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T05:55:54Z","timestamp":1695880554000},"page":"1740","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Utilizing a Combination of Network Pharmacology and Experimental Validation to Unravel the Mechanism by Which Kuanxiongzhuyu Decoction Ameliorates Myocardial Infarction Damage"],"prefix":"10.3390","volume":"59","author":[{"given":"Yihao","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Cardiology, Affiliated Hangzhou First People\u2019s Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaofu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Affiliated Hangzhou First People\u2019s Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liuying","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Affiliated Hangzhou First People\u2019s Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linhao","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Affiliated Hangzhou First People\u2019s Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China"},{"name":"Translational Medicine Research Center, Affiliated Hangzhou First People\u2019s Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yizhou","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Affiliated Hangzhou First People\u2019s Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yigang","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Affiliated Hangzhou First People\u2019s Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2122","DOI":"10.1016\/j.jacc.2020.02.059","article-title":"Triglyceride-Rich Lipoprotein Cholesterol, Small Dense LDL Cholesterol, and Incident Cardiovascular Disease","volume":"75","author":"Duran","year":"2020","journal-title":"J. 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