{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:59:59Z","timestamp":1760144399645,"version":"build-2065373602"},"reference-count":39,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T00:00:00Z","timestamp":1713484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"ERAF","doi-asserted-by":"publisher","award":["1.1.1.2\/ VIAA\/4\/20\/626","ES RTD\/2018\/29"],"award-info":[{"award-number":["1.1.1.2\/ VIAA\/4\/20\/626","ES RTD\/2018\/29"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"name":"ERA-NET Neuron consortium","award":["1.1.1.2\/ VIAA\/4\/20\/626","ES RTD\/2018\/29"],"award-info":[{"award-number":["1.1.1.2\/ VIAA\/4\/20\/626","ES RTD\/2018\/29"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objectives. Neurogenesis is an integral process in post-stroke recovery, involving the recruitment of proliferating neuroblasts from neurogenic niches of the mammal brain. However, the role of neurogenesis in the long-term restoration following ischemic stroke is fragmented. Post-stroke motor dysfunction includes challenges in the proper, coordinated use of hands and is present in roughly two-thirds of human patients. In this study, we investigated chronic behavioral and biochemical alterations after transient cerebral ischemia in adult male mice. Materials and Methods: Twelve-week-old C57BL\/6N male mice were used, and fMCAo lasting 60 min was induced. At multiple timepoints after fMCAo induction, a single pellet reaching task was performed. Six months after the procedure, we immunohistochemically determined the number of proliferating neuroblasts (BrdU and DCX-positive) and the number of differentiated astrocytes (GFAP-positive) in both brain hemispheres. Results: The reaching ability of fMCAo mice was impaired from one month to six months after the induction of ischemia. Neuroblast proliferation was increased in the ipsilateral SVZ, whereas GFAP+ cell count was elevated in the hippocampal DG of both hemispheres of the fMCAo group mice. Conclusions: Our current report demonstrates the long-term effects of transient cerebral ischemia on mice functional parameters and neurogenesis progression. Our data demonstrate that transient cerebral ischemia promotes a long-lasting regenerative response in the ipsilateral brain hemisphere, specifically in the neurogenic SVZ and DG regions.<\/jats:p>","DOI":"10.3390\/medicina60040658","type":"journal-article","created":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T06:28:09Z","timestamp":1713508089000},"page":"658","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Long-Term Alterations in Motor Skills, Neurogenesis and Astrocyte Numbers following Transient Cerebral Ischemia in Mice"],"prefix":"10.3390","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7098-7483","authenticated-orcid":false,"given":"Vladimirs","family":"Pilipenko","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine, University of Latvia, Raina Blvd. 19, LV-1586 Riga, Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jolanta","family":"Upite","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine, University of Latvia, Raina Blvd. 19, LV-1586 Riga, Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatrise Luize","family":"Revina","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine, University of Latvia, Raina Blvd. 19, LV-1586 Riga, Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4062-3363","authenticated-orcid":false,"given":"Baiba","family":"Jansone","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine, University of Latvia, Raina Blvd. 19, LV-1586 Riga, Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1161\/STROKEAHA.115.011932","article-title":"Modeling Ischemic Stroke In Vitro: Status Quo and Future Perspectives","volume":"47","author":"Holloway","year":"2016","journal-title":"Stroke"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2200","DOI":"10.1111\/j.1582-4934.2010.01135.x","article-title":"Neuroprotection in acute ischemic stroke\u2014Current status","volume":"14","author":"Auriel","year":"2010","journal-title":"J. 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