{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T07:32:33Z","timestamp":1789025553045,"version":"build-2803163510"},"reference-count":26,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research University Grant, Faculty of Dentistry, University of Malaya","award":["GPF008E-2019"],"award-info":[{"award-number":["GPF008E-2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objectives: Mucin has been implicated via various mechanisms in the development and growth of tumour cells. However, mucin expression studies in salivary gland tumours are limited, especially with samples from minor salivary glands. This study aims to investigate and compare mucin expression in benign and malignant salivary gland tumours of minor and major salivary gland origins. Materials and Methods: Special stains were used to stain neutral mucin (Periodic acid Schiff), sialomucin (Alcian Blue) and sulfomucin (Aldehyde Fuschin) within tissues from six normal salivary glands and 73 salivary gland tumours including 31 pleomorphic adenomas, 27 mucoepidermoid carcinomas, and 15 adenoid cystic carcinomas. A semi-quantitative approach was used to evaluate mucin expression within ductal lumens. Sialomucin was the most expressed mucin in all salivary gland tumours, regardless of origin. Results: A significant difference was observed in the mucin expression between benign and malignant salivary gland tumours, as pleomorphic adenoma showed three times significantly higher expression of sialomucin compared to mucoepidermoid carcinoma and adenoid cystic carcinoma (p = 0.028). Pleomorphic adenomas of major glands showed 42 times significantly higher expression of sialomucin compared to those of minor glands (p = 0.000). Conclusions: Sialomucin content in pleomorphic adenomas of major glands was vastly increased compared to that in minor glands. Differential sialomucin expression in benign and malignant salivary gland tumours suggests a role in diagnosing of borderline salivary gland tumours.<\/jats:p>","DOI":"10.3390\/medicina60060920","type":"journal-article","created":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T12:19:46Z","timestamp":1717417186000},"page":"920","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Differential Expression of Mucin in Salivary Gland Tumours"],"prefix":"10.3390","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7424-5938","authenticated-orcid":false,"given":"Nurul Inaas","family":"Mahamad Apandi","sequence":"first","affiliation":[{"name":"Fakulti Pergigian, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9889-3526","authenticated-orcid":false,"given":"Siew Wui","family":"Chan","sequence":"additional","affiliation":[{"name":"Fakulti Pergigian, Universiti Malaya, Kuala Lumpur 50603, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yen Fa","family":"Toh","sequence":"additional","affiliation":[{"name":"Jabatan Patologi, Pusat Perubatan Universiti Malaya, Kuala Lumpur 50603, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"46","DOI":"10.4103\/0973-029X.203762","article-title":"Histopathological study of salivary gland tumors","volume":"21","author":"Bobati","year":"2017","journal-title":"J. Oral. Maxillofac. Pathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"13079","DOI":"10.14260\/jemds\/2014\/3739","article-title":"Histochemical Study of Mucosubstances of Normal Human Parotid Salivary Gland","volume":"3","author":"Rohini","year":"2014","journal-title":"J. Evol. Med. Dent. Sci."},{"key":"ref_3","first-page":"3450","article-title":"Histochemical Study of Salivary Mucins in Normal and Neoplastic Salivary Glands","volume":"4","author":"Naag","year":"2010","journal-title":"J. Clin. Diagn. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/s10555-018-09775-0","article-title":"Cancer-associated Mucins: Role in Immune Modulation and Metastasis","volume":"38","author":"Bhatia","year":"2019","journal-title":"Cancer Metastasis Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.trsl.2015.03.004","article-title":"Mitogen-activated Protein Kinase Inhibition Reduces Mucin 2 Production and Mucinous Tumor Growth","volume":"166","author":"Ashok","year":"2015","journal-title":"Transl. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1159\/000313703","article-title":"Introduction to Salivary Glands: Structure, Function and Embryonic Development. Salivary Glands. Development, Adaptations and Disease","volume":"14","author":"Miletich","year":"2010","journal-title":"Front. Oral. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Rekthman, N., and Bishop, J.A. (2011). Quick Refernce Handbook for Surgical Pathologists, Springer.","DOI":"10.1007\/978-3-642-20086-1"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1136\/jcp.46.11.1011","article-title":"Histochemistry of Mucin Secreting Components in Mucoepidermoid and Adenosquamous Carcinoma of Oesophagus","volume":"46","author":"Lam","year":"1993","journal-title":"J. Clin. Pathol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1007\/BF01004186","article-title":"Histochemical Studies on Pseudocyst in Adenoid Cystic Carcinoma of the Human Salivary Gland","volume":"17","author":"Toida","year":"1985","journal-title":"Histochem. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"221","DOI":"10.4103\/0970-9371.103938","article-title":"The Utility Periodic Acid Schiff with Diastase and Alcian Blue Stains on Fine Needle Aspirates of Breast and Salivary Gland Neoplasms","volume":"29","author":"Panicker","year":"2012","journal-title":"J. Cytol."},{"key":"ref_11","first-page":"407","article-title":"Alcian Blue Technics for the Histochemical Study of Acid Carbohydrates","volume":"4","author":"Mowry","year":"1956","journal-title":"J. Histochem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1093\/ajcp\/33.5_ts.453","article-title":"Histochemical Differentiation of Acid Mucopolysaccharides By Means of Combined Aldehyde Fuchsin-Alcian Blue Staining","volume":"33","author":"Spicer","year":"1960","journal-title":"Am. J. Clin. Pathol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1002\/1097-0142(197408)34:2<282::AID-CNCR2820340211>3.0.CO;2-W","article-title":"Abnormal Patterns of Mucus Secretion in Apparently Normal Mucosa of Large Intestine with Carcinoma","volume":"34","author":"Filipe","year":"1974","journal-title":"Cancer"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1136\/jcp.2003.014043","article-title":"Mucin Expression in Pleomorphic Adenoma of Salivary Gland: A Potential Role for MUC1 as a Marker to Predict Recurrence","volume":"57","author":"Hamada","year":"2004","journal-title":"J. Clin. Pathol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1067\/mhn.2001.112575","article-title":"MUC4 (Sialomucin Complex) Expression in Salivary Gland Tumors and Squamous Cell Carcinoma of the Upper Aerodigestive Tract","volume":"124","author":"Weed","year":"2016","journal-title":"Otolaryngol.\u2014Head. Neck Surg."},{"key":"ref_16","unstructured":"(1981). Histochemical Study of Salivary Gland Tumors. J. Pathol. Transl. Med., 15, 53\u201361."},{"key":"ref_17","unstructured":"Barnes, L. (2001). Diseases of salivary glands. Surgical Pathology of the Head and Neck, Marcel Dekker Inc."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"404","DOI":"10.4103\/0976-9668.136207","article-title":"Diagnostic and Prognostic Significance of Different Mucin Expression, Preoperative CEA, and CA-125 in Colorectal Carcinoma: A Clinicopathological Study","volume":"5","author":"Jain","year":"2014","journal-title":"J. Nat. Sci. Biol. Med."},{"key":"ref_19","first-page":"161","article-title":"Pathophysiology of GIT mucins","volume":"29","author":"Gad","year":"1982","journal-title":"Adv. Physio Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1097\/01.pas.0000155856.84553.c9","article-title":"Expression of Membrane-Bound Mucins (MUC1 and MUC4) and Secreted Mucins (MUC2, MUC5AC, MUC5B, MUC6 and MUC7) in Mucoepidermoid Carcinomas of Salivary Glands","volume":"29","author":"Alos","year":"2005","journal-title":"Am. J. Surg. Pathol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1038\/nm0198-043","article-title":"Cancer-associated MUCl Mucin Inhibits Human T-cell Proliferation, Which is Reversible by IL-2","volume":"4","author":"Agrawal","year":"1998","journal-title":"Nat. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"379","DOI":"10.4103\/0973-029X.151319","article-title":"Epithelial and Stromal Patterns of Pleomorphic Adenoma of Minor Salivary Glands: A Histopathological and Histochemical study","volume":"18","author":"Satpathy","year":"2014","journal-title":"J. Oral Maxillofac. Pathol. JOMFP"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1007\/s12105-013-0473-9","article-title":"Schwannoma-like Pleomorphic Adenoma: A Case Report with Review of the Literature","volume":"8","author":"Lombardi","year":"2014","journal-title":"Head Neck Pathol."},{"key":"ref_24","first-page":"159","article-title":"Mucin Secretion Activity of Gastric Cancer as a Prognostic Factor: A Clinicopathological Analysis","volume":"16","author":"Huszno","year":"2012","journal-title":"Contemp. Oncol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.otc.2021.02.003","article-title":"Incorporating Sialendoscopy into the Otolaryngology Clinic","volume":"54","author":"Badger","year":"2021","journal-title":"Otolaryngol. Clin. N. Am."},{"key":"ref_26","first-page":"144","article-title":"Different Types of Papillae and Their Treatment","volume":"Volume 1","author":"Marchal","year":"2015","journal-title":"Sialendoscopy. The Hands-On Book"}],"container-title":["Medicina"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1648-9144\/60\/6\/920\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:52:01Z","timestamp":1760107921000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1648-9144\/60\/6\/920"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,31]]},"references-count":26,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,6]]}},"alternative-id":["medicina60060920"],"URL":"https:\/\/doi.org\/10.3390\/medicina60060920","relation":{},"ISSN":["1648-9144"],"issn-type":[{"value":"1648-9144","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,31]]}}}