{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T03:09:44Z","timestamp":1780456184401,"version":"3.54.1"},"reference-count":46,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,10,20]],"date-time":"2024-10-20T00:00:00Z","timestamp":1729382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007071","name":"Harran University","doi-asserted-by":"publisher","award":["18146"],"award-info":[{"award-number":["18146"]}],"id":[{"id":"10.13039\/501100007071","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objectives: Atherosclerosis, driven by dyslipidaemia and oxidative stress, is a leading cause of cardiovascular morbidity and mortality. This study evaluates the effects of vigorous-intensity bodybuilding exercise (VIBBE) on atherosclerosis biomarkers\u2014including paraoxonase-1 (PON1) and arylesterase (ARE) activities\u2014and lipid profiles in male bodybuilders who do not use anabolic-androgenic steroids. Comparisons were made with individuals engaged in moderate-intensity aerobic exercise (MIAE), as well as overweight\/obese sedentary (OOS) and normal-weight sedentary (NWS) individuals. Materials and Methods: A cross-sectional study was conducted involving 122 healthy male participants aged 18\u201345 years, divided into four groups: VIBBE (n = 31), OOS (n = 30), MIAE (n = 32), and NWS (n = 29). Anthropometric assessments were performed, and fasting blood samples were collected for biochemical analyses, including lipid profiles and PON1 and ARE activities. Statistical analyses compared the groups and evaluated correlations between adiposity measures and atherosclerosis biomarkers. Results: The VIBBE group exhibited significantly lower levels of low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), and logarithm of the TG to high-density lipoprotein cholesterol (HDL-C) ratio [log(TG\/HDL-C)] compared to the OOS group (p &lt; 0.05 for all), indicating improved lipid profiles. However, these improvements were not significant when compared to the NWS group (p &gt; 0.05), suggesting that VIBBE may not provide additional lipid profile benefits beyond those associated with normal weight status. PON1 and ARE activities were significantly lower in the VIBBE group compared to the MIAE group (p &lt; 0.05 for both), suggesting that VIBBE may not effectively enhance antioxidant defences. Correlation analyses revealed significant inverse relationships between PON1 and ARE activities and adiposity measures, including body mass index (BMI), waist-to-hip ratio (WHR), waist-to-height ratio (WHtR), body fat percentage (BFP), fat mass index (FMI), and obesity degree (OD) (p &lt; 0.05 for all). Positive correlations were observed between oxLDL and log(TG\/HDL-C) and adiposity measures (p &lt; 0.05 for all). Conclusions: Vigorous-intensity bodybuilding exercise improves certain lipid parameters compared to sedentary obese individuals but does not significantly enhance antioxidant enzyme activities or further improve lipid profiles beyond those observed in normal-weight sedentary men. Conversely, moderate-intensity aerobic exercise significantly enhances PON1 and ARE activities and improves lipid profiles, offering superior cardiovascular benefits. These findings underscore the importance of incorporating moderate-intensity aerobic exercise into physical activity guidelines to optimize cardiovascular health by balancing improvements in lipid metabolism with enhanced antioxidant defences.<\/jats:p>","DOI":"10.3390\/medicina60101717","type":"journal-article","created":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T03:57:09Z","timestamp":1729483029000},"page":"1717","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Assessment of Paraoxonase 1 and Arylesterase Activities and Lipid Profile in Bodybuilders: A Comparative Study of Physical Activity and Anthropometry on Atherosclerosis"],"prefix":"10.3390","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7565-3394","authenticated-orcid":false,"given":"Hakim","family":"Celik","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Harran University, Sanliurfa 63290, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2650-5475","authenticated-orcid":false,"given":"Mehmed Zahid","family":"Tuysuz","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Faculty of Medicine, Harran University, Sanliurfa 63290, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0147-9223","authenticated-orcid":false,"given":"Yakup","family":"Aktas","sequence":"additional","affiliation":[{"name":"Department Coaching Education, School of Physical Education and Sports, Harran University, Sanliurfa 63290, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet Ali","family":"Eren","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Faculty of Medicine, Harran University, Sanliurfa 63290, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7831-2715","authenticated-orcid":false,"given":"Recep","family":"Demirbag","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Faculty of Medicine, Harran University, Sanliurfa 63290, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zhu, G., Gao, Y., Qian, J., Lai, Y., Lin, H., Liu, C., Chen, F., and Liu, X. (2023). Comprehensive analysis of atherosclerotic plaques reveals crucial genes and molecular mechanisms associated with plaque progression and rupture. Front. Cardiovasc. Med., 10.","DOI":"10.3389\/fcvm.2023.951242"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.addr.2020.07.019","article-title":"Lipoproteins and lipids in cardiovascular disease: From mechanistic insights to therapeutic targeting","volume":"159","author":"Soppert","year":"2020","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.medici.2014.11.009","article-title":"Atherogenic index as a predictor of atherosclerosis in subjects with familial Mediterranean fever","volume":"50","author":"Acay","year":"2014","journal-title":"Medicina"},{"key":"ref_4","first-page":"40","article-title":"Pathogenesis of atherosclerosis: A multifactorial process","volume":"7","author":"Singh","year":"2002","journal-title":"Exp Clin Cardiol"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Vekic, J., Stromsnes, K., Mazzalai, S., Zeljkovic, A., Rizzo, M., and Gambini, J. (2023). Oxidative Stress, Atherogenic Dyslipidemia, and Cardiovascular Risk. Biomedicines, 11.","DOI":"10.3390\/biomedicines11112897"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1186\/s12944-021-01575-w","article-title":"Low-\/high-density lipoprotein cholesterol ratio and carotid plaques in patients with coronary heart disease: A Chinese cohort study","volume":"20","author":"Li","year":"2021","journal-title":"Lipids Health Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"5245308","DOI":"10.1155\/2020\/5245308","article-title":"Mechanistic Insights into the Oxidized Low-Density Lipoprotein-Induced Atherosclerosis","volume":"2020","author":"Khatana","year":"2020","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4264314","DOI":"10.1155\/2022\/4264314","article-title":"Paraoxonase-1 (PON-1) Arylesterase Activity Levels in Patients with Coronary Artery Disease: A Meta-Analysis","volume":"2022","author":"Zuin","year":"2022","journal-title":"Dis. Markers"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Erre, G.L., Bassu, S., Giordo, R., Mangoni, A.A., Carru, C., Pintus, G., and Zinellu, A. (2022). Association between Paraoxonase\/Arylesterase Activity of Serum PON-1 Enzyme and Rheumatoid Arthritis: A Systematic Review and Meta-Analysis. Antioxidants, 11.","DOI":"10.3390\/antiox11122317"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Otocka-Kmiecik, A., Or\u0142owska-Majdak, M., Stawski, R., Szkudlarek, U., Padula, G., Ga\u0142czy\u0144ski, S., and Nowak, D. (2023). Effect of Exercise Repetitions on Arylesterase Activity of PON1 in Plasma of Average-Trained Men\u2014The Dissociation between Activity and Concentration. Antioxidants, 12.","DOI":"10.3390\/antiox12061296"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Alves, R.C., Prestes, J., Enes, A., de Moraes, W.M.A., Trindade, T.B., de Salles, B.F., Aragon, A.A., and Souza-Junior, T.P. (2020). Training Programs Designed for Muscle Hypertrophy in Bodybuilders: A Narrative Review. Sports, 8.","DOI":"10.3390\/sports8110149"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.9734\/jalsi\/2020\/v23i330148","article-title":"Effect of Circuit Training on the Cardiovascular Endurance and Quality of Life: Findings from an Apparently Healthy Female Adult Population","volume":"23","author":"Ikenna","year":"2020","journal-title":"J. Appl. Life Sci. Int."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"18","DOI":"10.5114\/hm.2020.94199","article-title":"The impact of resistance training volume on muscle size and lean body mass: To infinity and beyond?","volume":"21","author":"Souza","year":"2020","journal-title":"Hum. Mov."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1249\/01.mss.0000191417.48710.11","article-title":"Exercise-induced changes in insulin action and glycogen metabolism in elderly adults","volume":"38","author":"Coker","year":"2006","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Santos, L.d.R., de Araujo, S.S., Vieira, E.F.d.S., Estevam, C.d.S., dos Santos, J.L., Wichi, R.B., Lima, F.B., Carvalho, C.R.O., Aidar, F.J., and Mar\u00e7al, A.C. (2020). Effects of 12 weeks of resistance training on cardiovascular risk factors in school adolescents. Medicina, 56.","DOI":"10.3390\/medicina56050220"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"E539","DOI":"10.1152\/ajpendo.00276.2014","article-title":"Metabolic and hormonal responses to isoenergetic high-intensity interval exercise and continuous moderate-intensity exercise","volume":"307","author":"Peake","year":"2014","journal-title":"Ame J Physiol. Endocrinol Metab."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1161\/CIRCRESAHA.118.312669","article-title":"Sedentary Behavior, Exercise, and Cardiovascular Health","volume":"124","author":"Lavie","year":"2019","journal-title":"Circ. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"111360","DOI":"10.1016\/j.exger.2021.111360","article-title":"A moderate intensity exercise program improves physical function and oxidative damage in older women with and without sarcopenic obesity","volume":"150","year":"2021","journal-title":"Exp. Gerontol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Bai, X., Soh, K.G., Dev, R.D.O., Talib, O., Xiao, W., and Cai, H. (2022). Effect of Brisk Walking on Health-Related Physical Fitness Balance and Life Satisfaction Among the Elderly: A Systematic Review. Front. Public Health, 9.","DOI":"10.3389\/fpubh.2021.829367"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e023386","DOI":"10.1161\/JAHA.121.023386","article-title":"Moderate- and High-Intensity Exercise Improves Lipoprotein Profile and Cholesterol Efflux Capacity in Healthy Young Men","volume":"11","author":"Stanton","year":"2022","journal-title":"J. Am. Heart Assoc."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e46131","DOI":"10.5812\/semj.46131","article-title":"The effect of exercise training type on paraoxonase-1 and lipid profile in rats","volume":"18","author":"TaheriChadorneshin","year":"2017","journal-title":"Shiraz E-Medical J."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Reichert, C.O., Levy, D., and Bydlowski, S.P. (2021). Paraoxonase role in human neurodegenerative diseases. Antioxidants, 10.","DOI":"10.3390\/antiox10010011"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"108067","DOI":"10.1016\/j.diabres.2020.108067","article-title":"Importance of paraoxonase 1 (PON1) as an antioxidant and antiatherogenic enzyme in the cardiovascular complications of type 2 diabetes: Genotypic and phenotypic evaluation","volume":"161","author":"Shokri","year":"2020","journal-title":"Diabetes Res. Clin. Pract."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Casta\u00f1\u00e9, H., Jim\u00e9nez-Franco, A., Mart\u00ednez-Navidad, C., Placed-Gallego, C., Cambra-Cort\u00e9s, V., Perta, A.-M., Par\u00eds, M., del Castillo, D., Arenas, M., and Camps, J. (2023). Serum Arylesterase, Paraoxonase, and Lactonase Activities and Paraoxonase-1 Concentrations in Morbidly Obese Patients and Their Relationship with Non-Alcoholic Steatohepatitis. Antioxidants, 12.","DOI":"10.3390\/antiox12122038"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Watanabe, J., Kotani, K., and Gugliucci, A. (2023). Paraoxonase 1 and Chronic Kidney Disease: A Meta-Analysis. J. Clin. Med., 12.","DOI":"10.3390\/jcm12031199"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.beem.2013.01.004","article-title":"Impact of obesity on cardiovascular health","volume":"27","author":"Chrostowska","year":"2013","journal-title":"Best Pract. Res. Clin. Endocrinol. Metab."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3803\/EnM.2022.101","article-title":"The Influence of Obesity and Metabolic Health on Vascular Health","volume":"37","author":"Rhee","year":"2022","journal-title":"Endocrinol. Metab."},{"key":"ref_28","first-page":"241","article-title":"Carotid intima media-thickness is increased in obese children metabolically healthy, metabolically unhealthy, and with metabolic syndrome, compared to the non-obese controls","volume":"25","author":"Farello","year":"2021","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1080\/08830185.2019.1638921","article-title":"Inflammatory processes in obesity: Focus on endothelial dysfunction and the role of adipokines as inflammatory mediators","volume":"38","author":"Singh","year":"2019","journal-title":"Int. Rev. Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.amepre.2011.09.023","article-title":"Sedentary behavior and adiposity-associated inflammation: The multi-ethnic study of atherosclerosis","volume":"42","author":"Allison","year":"2012","journal-title":"Am. J. Prev. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/j.metabol.2005.11.004","article-title":"Body surface area in normal-weight, overweight, and obese adults. A comparison study","volume":"55","author":"Verbraecken","year":"2006","journal-title":"Metabolism"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Merchant, R.A., Seetharaman, S., Au, L., Wong, M.W.K., Wong, B.L.L., Tan, L.F., Chen, M.Z., Ng, S.E., Soong, J.T.Y., and Hui, R.J.Y. (2021). Relationship of Fat Mass Index and Fat Free Mass Index With Body Mass Index and Association With Function, Cognition and Sarcopenia in Pre-Frail Older Adults. Front. Endocrinol., 12.","DOI":"10.3389\/fendo.2021.765415"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Brugnara, L., Murillo, S., Novials, A., Rojo-Mart\u00ednez, G., Soriguer, F., Goday, A., Calle-Pascual, A., Casta\u00f1o, L., Gaztambide, S., and Vald\u00e9s, S. (2016). Low physical activity and its association with diabetes and other cardiovascular risk factors: A nationwide, population-based study. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0160959"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1136\/bjsports-2020-102955","article-title":"World Health Organization 2020 guidelines on physical activity and sedentary behaviour","volume":"54","author":"Bull","year":"2020","journal-title":"Br. J. Sports Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3547","DOI":"10.1093\/eurheartj\/ehz564","article-title":"Mortality reduction with physical activity in patients with and without cardiovascular disease","volume":"40","author":"Jeong","year":"2019","journal-title":"Eur. Heart J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.ygyno.2008.10.031","article-title":"Serum paraoxonase and arylesterase activities in patients with epithelial ovarian cancer","volume":"112","author":"Camuzcuoglu","year":"2009","journal-title":"Gynecol. Oncol."},{"key":"ref_37","first-page":"2517","article-title":"Serum paraoxonase, arylesterase activities and oxidative status in patients with insomnia","volume":"17","author":"Liang","year":"2013","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"146","DOI":"10.2478\/enr-2018-0018","article-title":"Resistance training alone or combined with leucine supplementation improves the serum lipid profile of diabetic rats, whereas leucine alone does not","volume":"52","author":"Martins","year":"2018","journal-title":"Endocr. Regul."},{"key":"ref_39","first-page":"1","article-title":"Raising the Bar for Public Health: Resistance Training and Health Benefits","volume":"3","author":"Tyler","year":"2023","journal-title":"Int. J. Strength Cond."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1139\/apnm-2018-0430","article-title":"Oxidative stress, inflammation, psychological status, and severity of respiratory infections are negatively affected during the pre-contest period in amateur bodybuilders","volume":"44","author":"Schoenfeld","year":"2019","journal-title":"Appl. Physiol. Nutr. Metab."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1097\/WNQ.0b013e3181a36481","article-title":"Effect of regular exercise on serum paraoxonase and arylesterase related to age increment in the male rat","volume":"19","author":"Asghari","year":"2009","journal-title":"Neurosurg. Q."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1007\/s00421-006-0369-4","article-title":"Mild exercise training, cardioprotection and stress genes profile","volume":"99","author":"Marini","year":"2007","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1249\/MSS.0000000000000499","article-title":"Discrete features of sedentary behavior impact cardiometabolic risk factors","volume":"47","author":"Lyden","year":"2015","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3404","DOI":"10.1093\/eurheartj\/ehab518","article-title":"Obesity and cardiovascular health: The size of the problem","volume":"42","author":"Rosengren","year":"2021","journal-title":"Eur. Heart J."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.jacl.2016.10.014","article-title":"Influence of sedentary behavior, physical activity, and cardiorespiratory fitness on the atherogenic index of plasma","volume":"11","author":"Edwards","year":"2017","journal-title":"J. Clin. Lipidol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.bbrc.2010.05.120","article-title":"Estradiol enhances cell-associated paraoxonase 1 (PON1) activity in vitro without altering PON1 expression","volume":"397","author":"Ahmad","year":"2010","journal-title":"Biochem. Biophys. Res. Commun."}],"container-title":["Medicina"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1648-9144\/60\/10\/1717\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:17:05Z","timestamp":1760113025000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1648-9144\/60\/10\/1717"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,20]]},"references-count":46,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2024,10]]}},"alternative-id":["medicina60101717"],"URL":"https:\/\/doi.org\/10.3390\/medicina60101717","relation":{},"ISSN":["1648-9144"],"issn-type":[{"value":"1648-9144","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,20]]}}}