{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T19:08:18Z","timestamp":1763665698123,"version":"build-2065373602"},"reference-count":42,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T00:00:00Z","timestamp":1740700800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background\/Objectives: In the genus Allium, it has been shown that some organosulfate compounds of common garlic and onion have an antiplatelet effect. Still, little is known about the anticoagulant activity of the species, Allium ampeloprasum L., specifically Snow mountain garlic; due to its growth site at 6000 m above sea level at temperatures of \u221210 \u00b0C, it could contain different organosulfate compounds. Methods: This study evaluated the anticoagulant effect of a lyophilized aqueous extract of S. mountain garlic in vivo, toxicity in male Wistar rats for 90 days, and the organosulfate compounds present. Results: There was a significant increase (p &lt; 0.05) in the area under the curve for bleeding time at doses of 500 and 1000 mg\/kg\/d of lyophilized aqueous extract of Snow mountain garlic and 100 mg\/kg\/d of acetylsalicylic acid. The ED50 was 366 \u00b1 2.43 mg\/kg. For chronic toxicity in vivo, no histopathological alterations were found at doses below 500 mg\/kg. On the other hand, organosulfate compounds were found in the lyophilized aqueous extract of S. mountain garlic that had not been documented to have an anticoagulant effect. Conclusions: We conclude that S. mountain garlic contains anticoagulant compounds which may be candidates for the treatment of thrombosis.<\/jats:p>","DOI":"10.3390\/medicina61030429","type":"journal-article","created":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T06:45:42Z","timestamp":1740725142000},"page":"429","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Preclinical Evaluation of the Anticoagulant Effect of an Aqueous Extract of Snow Mountain Garlic"],"prefix":"10.3390","volume":"61","author":[{"given":"Isabel","family":"Clark-Montoya","sequence":"first","affiliation":[{"name":"Faculty of Chemical Sciences, Autonomous University of San Luis Potosi, Dr. Manuel Nava Mart\u00ednez Avenue #6, University Zone, San Luis Potosi 78210, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa del Carmen","family":"Mil\u00e1n-Segovia","sequence":"additional","affiliation":[{"name":"Faculty of Chemical Sciences, Autonomous University of San Luis Potosi, Dr. Manuel Nava Mart\u00ednez Avenue #6, University Zone, San Luis Potosi 78210, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Obed","family":"Lemus-Rojero","sequence":"additional","affiliation":[{"name":"Histology and Histopathology Laboratory, Academic Unit of Dentistry, Autonomous University of Zacatecas, Zacatecas 98160, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5325-3760","authenticated-orcid":false,"given":"Osmar Antonio","family":"Jaramillo-Morales","sequence":"additional","affiliation":[{"name":"Division of Life Sciences, Nursing and Obstetrics Department, Campus Irapuato-Salamanca, Guanajuato University, Guanajuato 36612, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bertha","family":"J\u00faarez-Flores","sequence":"additional","affiliation":[{"name":"Desert Areas Research Institute, Autonomous University of San Luis Potosi, San Luis Potosi 78290, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6118-6393","authenticated-orcid":false,"given":"Yolanda","family":"Ter\u00e1n-Figueroa","sequence":"additional","affiliation":[{"name":"Faculty of Nursing and Nutrition, Autonomous University of San Luis Potosi, Ni\u00f1o Artillero Avenue #130, University Zone, San Luis Potosi 78240, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Heestermans, M., Poenou, G., Hamzeh-Cognasse, H., Cognasse, F., and Bertoletti, L. (2022). Anticoagulants: A Short History, Their Mechanism of Action, Pharmacology, and Indications. Cells, 11.","DOI":"10.3390\/cells11203214"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1111\/j.0960-135X.2005.01452.x","article-title":"From heparins to factor Xa inhibitors and beyond","volume":"35","author":"Alban","year":"2005","journal-title":"Eur. J. Clin. Investig."},{"key":"ref_3","first-page":"434","article-title":"Heparin an anticoagulant","volume":"63","author":"Howell","year":"1992","journal-title":"Am. J. Physiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1161\/01.CIR.19.1.97","article-title":"The Discovery of Dicumarol and Its Sequels","volume":"19","author":"Link","year":"1959","journal-title":"Circulation"},{"key":"ref_5","first-page":"531","article-title":"Choosing Non-Vitamin K Antagonist Oral Anticoagulants: Practical Considerations We Need to Know","volume":"16","author":"Amin","year":"2016","journal-title":"Ochsner J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/S1138-3593(13)74376-4","article-title":"Nuevos anticoagulantes frente a anticoagulantes cl\u00e1sicos: Ventajas e inconvenientes","volume":"39","year":"2013","journal-title":"Semergen"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Willers, A., Arens, J., Mariani, S., Pels, H., Maessen, J.G., Hackeng, T.M., Lorusso, R., and Swol, J. (2021). New Trends, Advantages and Disadvantages in Anticoagulation and Coating Methods Used in Extracorporeal Life Support Devices. Membranes, 11.","DOI":"10.3390\/membranes11080617"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.7326\/M22-0511","article-title":"Comparative Effectiveness and Safety Between Apixaban, Dabigatran, Edoxaban, and Rivaroxaban Among Patients with Atrial Fibrillation","volume":"175","author":"Lau","year":"2022","journal-title":"Ann. Intern. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1056\/NEJMoa1009638","article-title":"Investigators. Rivaroxaban versus warfarin in nonvalvular atrial brillation","volume":"365","author":"Patel","year":"2011","journal-title":"N. Engl. J. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1182\/blood-2023-184457","article-title":"A Comparison of Bleeding Events Among Patients on Apixaban, Rivaroxaban, and Warfarin for Atrial Fibrillation and\/or Venous Thromboembolism","volume":"142","author":"Schaefer","year":"2023","journal-title":"Blood"},{"key":"ref_11","unstructured":"World Health Organization (2024, July 23). WHO Strategy on Traditional Medicine 2014\u20132023. Available online: https:\/\/www.who.int\/es\/publications\/i\/item\/9789241506096."},{"key":"ref_12","first-page":"101","article-title":"World Health Organization Strategies in Traditional Medicine and Recognition of Traditional Medicine Systems","volume":"13","year":"2020","journal-title":"Rev. Cuerpo M\u00e9dico. HNAAA"},{"key":"ref_13","first-page":"386","article-title":"Pharmacological effect of Allium sativum on coagulation, blood pressure, diabetic nephropathy, neurological disorders, spermatogenesis, antibacterial effects","volume":"4","author":"Abbas","year":"2019","journal-title":"Agric. Food"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1021\/jf062587s","article-title":"Effect of Cooking on Garlic (Allium sativum L.) Antiplatelet Activity and Thiosulfinates Content","volume":"55","author":"Cavagnaro","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1002\/mnfr.200700058","article-title":"Effects of garlic on platelet biochemistry and physiology","volume":"51","author":"Rahman","year":"2007","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_16","first-page":"128","article-title":"Effect of ingestion of raw garlic on serum cholesterol level, clotting time and fibrinolytic activity in normal subjects","volume":"37","author":"Gadkari","year":"1991","journal-title":"J. Postgrad. Med."},{"key":"ref_17","first-page":"1","article-title":"Effect of Allium sativum (Garlic) Extract on Blood Coagulation and Fibrinolysis","volume":"2","author":"Narsimha","year":"2017","journal-title":"Adv. Pharmacol. Clin. Trials"},{"key":"ref_18","first-page":"301","article-title":"Antihaemostatic effect of combination of Allium sativum L., ethanol extract and warfarin in Wistar rats","volume":"20","author":"Musubika","year":"2015","journal-title":"Rev. Cuba. De Plantas Med."},{"key":"ref_19","first-page":"1256","article-title":"In Vitro anticoagulant activity of Allium sativum plant extract","volume":"6","author":"Vaijayanthimala","year":"2017","journal-title":"World J. Pharm. Pharm. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/j.fct.2006.10.005","article-title":"Effect of diallyl trisulfide-rich garlic oil on blood coagulation and plasma activity of anticoagulation factors in rats","volume":"45","author":"Chan","year":"2007","journal-title":"Food Chem. Toxicol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1271\/bbb.60380","article-title":"Antithrombotic Effects of Odorless Garlic Powder Both in Vitro and in Vivo","volume":"71","author":"Fukao","year":"2007","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10540-006-9004-1","article-title":"Anticoagulant Modulation of Blood Cells and Platelet Reactivity by Garlic Oil in Experimental Diabetes Mellitus","volume":"26","author":"Ohaeri","year":"2006","journal-title":"Biosci. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1055\/s-2006-961378","article-title":"Comparative Pharmacological Investigations of Allium ursinum and Allium sativum","volume":"58","author":"Sendl","year":"1992","journal-title":"Planta Medica"},{"key":"ref_24","first-page":"107","article-title":"Pharmacological Properties of Allium cepa, Preclinical and Clinical Evidences; A Review","volume":"20","author":"Kianian","year":"2021","journal-title":"Iran J. Pharm. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1111\/jam.15537","article-title":"A comparative study of in-vitro and in-silico anti-candidal activity and GC\u2013MS profiles of snow mountain garlic vs. normal garlic","volume":"133","author":"Kaur","year":"2022","journal-title":"J. Appl. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"115939","DOI":"10.1016\/j.jep.2022.115939","article-title":"Validation of traditional claims of anti- arthritic efficacy of trans-Himalayan Snow mountain garlic (Allium ampeloprasum L.) extract using adjuvant-induced arthritis rat model: A comparative evaluation with normal garlic (Allium sativum L.) and dexamethasone","volume":"303","author":"Kaur","year":"2023","journal-title":"J. Ethnopharmacol."},{"key":"ref_27","first-page":"11","article-title":"Local antinociceptive effect of \u201cSnow mountain garlic\u201d in an experimental formalin model","volume":"71","year":"2023","journal-title":"Farm. J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1007\/978-1-4939-3332-7_23","article-title":"In Vitro and Cryopreservation Techniques for Conservation of Snow mountain Garlic","volume":"1391","author":"Mahajan","year":"2016","journal-title":"Methods Mol. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.ygeno.2019.07.014","article-title":"Transcriptome analysis of Snow Mountain Garlic for unraveling the organosulfur metabolic pathway","volume":"112","author":"Mehra","year":"2020","journal-title":"Genomics"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Shang, A., Cao, S.-Y., Xu, X.-Y., Gan, R.-Y., Tang, G.-Y., Corke, H., Mavumengwana, V., and Li, H.-B. (2019). Bioactive Compounds and Biological Functions of Garlic (Allium sativum L.). Foods, 8.","DOI":"10.3390\/foods8070246"},{"key":"ref_31","first-page":"39","article-title":"Therapeutic Effects of Garlic (Allium Sativum)","volume":"3","year":"2016","journal-title":"Salud Y Adm."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Uchechi, H., Atangwho, I., Kufre, E., and Ifeyinwa, O. (2016). Haemostatic property of Chromolaena odorata leaf extracts: In vitro and in vivo evaluation in wistar rats. J. Biol. Res., 89, Available online: https:\/\/api.semanticscholar.org\/CorpusID:55843032.","DOI":"10.4081\/jbr.2016.6211"},{"key":"ref_33","unstructured":"(2001). Technical Specifications for the Production, Care and Use of Laboratory Animals (Standard No. NOM-062-ZOO-1999). Available online: http:\/\/dof.gob.mx\/nota_detalle.php?codigo=764738&fecha=18\/06\/2001."},{"key":"ref_34","first-page":"189","article-title":"Hermatological study in the Wistar rat","volume":"32","author":"Orta","year":"1989","journal-title":"Rev. Fac. Med. UNAM"},{"key":"ref_35","first-page":"51","article-title":"Preclinical Evaluation of Hepatoprotective Activity of Ocimum basilicum L. and Allium sativum L","volume":"12","author":"Boffill","year":"2015","journal-title":"MediSur"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"71","DOI":"10.2147\/IBPC.S51434","article-title":"Potential of garlic (Allium sativum) in lowering high blood pressure: Mechanisms of action and clinical relevance","volume":"7","author":"Ried","year":"2014","journal-title":"Integr. Blood Press. Control"},{"key":"ref_37","first-page":"6825","article-title":"Induction of apoptosis by the garlic-derived compound S-allylmercaptocysteine (SAMC) is associated with microtubule depolymerization and c-Jun NH(2)-terminal kinase 1 activation","volume":"63","author":"Xiao","year":"2003","journal-title":"Cancer Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1002\/em.20474","article-title":"Biological properties of garlic and garlic-derived organosulfur compounds","volume":"50","author":"Iciek","year":"2009","journal-title":"Environ. Mol. Mutagen."},{"key":"ref_39","first-page":"335","article-title":"Resolution of left ventricular thrombus with apixaban in a patient with hypertrophic cardiomyopathy","volume":"44","author":"Hayiroglu","year":"2016","journal-title":"Turk. Kardiyol. Dern. Ars."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s12185-020-03029-y","article-title":"COVID-19-associated coagulopathy and disseminated intravascular coagulation","volume":"113","author":"Asakura","year":"2021","journal-title":"Int. J. Hematol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Clark-Montoya, I., Ter\u00e1n-Figueroa, Y., de Loera, D., Gayt\u00e1n-Hern\u00e1ndez, D., Alegr\u00eda-Torres, J.A., and Mil\u00e1n-Segovia, R.d.C. (2024). Anticoagulant Effect of Snow mountain garlic: In Vitro Evaluation of Aqueous Extract. Molecules, 29.","DOI":"10.3390\/molecules29204958"},{"key":"ref_42","unstructured":"Genetic Alliance, and The New York-Mid-Atlantic Consortium for Genetic and Newborn Screening Services (2024, July 27). Appendix M: Pharmacogenomics and Pharmacogenetics, Understanding Genetics: A Guide for Patients and Medical Professionals in the New York and Mid-Atlantic Region, Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK132199\/."}],"container-title":["Medicina"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1648-9144\/61\/3\/429\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:44:20Z","timestamp":1760028260000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1648-9144\/61\/3\/429"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,28]]},"references-count":42,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["medicina61030429"],"URL":"https:\/\/doi.org\/10.3390\/medicina61030429","relation":{},"ISSN":["1648-9144"],"issn-type":[{"type":"electronic","value":"1648-9144"}],"subject":[],"published":{"date-parts":[[2025,2,28]]}}}