{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T12:31:03Z","timestamp":1764937863642,"version":"build-2065373602"},"reference-count":66,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T00:00:00Z","timestamp":1742947200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objectives: Several micro- and macro-nutrient malnutrition states that are routinely assessed during clinical care of women in the antenatal period have been proposed as risk factors for preeclampsia. However, there is a paucity of data on the potential use of these biomarkers for detection of preeclampsia. The aim of this case-control study was to investigate the association of biomarkers from routine clinical tests, and those specific to micro- and macro-nutrient malnutrition, with the risk of preeclampsia. Materials and Methods: Venous blood samples of 250 participants with preeclampsia and 150 pregnant women without preeclampsia were collected and assayed immediately for the full blood count, urea and electrolytes, high-density cholesterol (HDL), total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL), oxidized low-density lipoprotein cholesterol (OxLDL), and selenium, in addition to urine iodine concentration (UIC). Results: The serum potassium\/magnesium ratio (K+\/Mg2+), UIC, fasting plasma glucose (FPG), thyroid stimulating hormone (TSH), lymphocyte percentage (L\/WBC%), and the oxidized LDL\/albumin ratio (OxLDL\/Alb) were identified as independent predictors of preeclampsia. Conclusions: Serum potassium\/magnesium ratio and other analytes essential for various biological processes, some of which are assayed during routine care, were significantly associated with preeclampsia, warranting further exploration as potential screening biomarkers in low-resource settings.<\/jats:p>","DOI":"10.3390\/medicina61040600","type":"journal-article","created":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T10:47:40Z","timestamp":1743072460000},"page":"600","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Circulating Potassium\/Magnesium Ratio, Thyroid Stimulating Hormone, Fasting Plasma Glucose, Oxidized LDL\/Albumin Ratio, and Urinary Iodine Concentration Are Possible Entities for Screening for Preeclampsia in Low-Resource Settings"],"prefix":"10.3390","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8393-1198","authenticated-orcid":false,"given":"Charles Bitamazire","family":"Businge","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Walter Sisulu University, Mthatha 5117, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin","family":"Longo-Mbenza","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of the Congo"},{"name":"Department of Public Health, Lomo University of Research, 652 Freesias, Kinshasa, Democratic Republic of the Congo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andre Pascal","family":"Kengne","sequence":"additional","affiliation":[{"name":"African Population Health Research Center (APHRC), Nairobi 00100, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1016\/j.jacc.2020.08.014","article-title":"Preeclampsia\u2014Pathophysiology and Clinical Presentations: JACC State-of-the-Art Review","volume":"76","author":"Ives","year":"2020","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S42","DOI":"10.1016\/j.placenta.2011.11.021","article-title":"Review: Biochemical markers to predict preeclampsia","volume":"33","author":"Anderson","year":"2012","journal-title":"Placenta"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.5114\/aoms.2020.97762","article-title":"Utility of biochemical tests in prediction, diagnostics and clinical management of preeclampsia: A review","volume":"16","author":"Kornacki","year":"2020","journal-title":"Arch. Med. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"S1071","DOI":"10.1016\/j.ajog.2020.07.020","article-title":"First trimester preeclampsia screening and prediction","volume":"226","author":"Chaemsaithong","year":"2022","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"89","DOI":"10.3892\/ijmm.2023.5292","article-title":"Predictive value of the sFlt-1\/PlGF ratio in women with suspected preeclampsia: An update (Review)","volume":"52","author":"Velegrakis","year":"2023","journal-title":"Int. J. Mol. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1002\/pd.2433","article-title":"Maternal plasma soluble fms-like tyrosine kinase-1 and free vascular endothelial growth factor at 11 to 13 weeks of gestation in preeclampsia","volume":"30","author":"Akolekar","year":"2010","journal-title":"Prenat. Diagn."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1002\/pd.4519","article-title":"First trimester screening for early and late preeclampsia based on maternal characteristics, biophysical parameters, and angiogenic factors","volume":"35","author":"Crovetto","year":"2015","journal-title":"Prenat. Diagn."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"102753","DOI":"10.1016\/j.jogc.2024.102753","article-title":"First-Trimester Soluble fms-like Tyrosine Kinase 1 (sFlt-1) for the Prediction of Preterm Preeclampsia","volume":"47","author":"Fillion","year":"2024","journal-title":"J. Obstet. Gynaecol. Can."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"608.e1\u2013608.e18","DOI":"10.1016\/j.ajog.2020.01.020","article-title":"A new model for screening for early-onset preeclampsia","volume":"222","author":"Serra","year":"2020","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_10","first-page":"215","article-title":"First trimester screening of serum soluble fms-like tyrosine kinase-1 and placental growth factor predicting hypertensive disorders of pregnancy","volume":"3","author":"Schneuer","year":"2013","journal-title":"Pregnancy Hypertens. Int. J. Women\u2019s Cardiovasc. Health"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"161.e1\u2013161.e11","DOI":"10.1016\/j.ajog.2009.09.016","article-title":"An automated method for the determination of the sFlt-1\/PIGF ratio in the assessment of preeclampsia","volume":"202","author":"Verlohren","year":"2010","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Nikuei, P., Rajaei, M., Roozbeh, N., Mohseni, F., Poordarvishi, F., Azad, M., and Haidari, S. (2020). Diagnostic accuracy of sFlt1\/PlGF ratio as a marker for preeclampsia. BMC Pregnancy Childbirth, 20.","DOI":"10.1186\/s12884-020-2744-2"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1111\/aogs.13540","article-title":"The role of angiogenic factors in the management of preeclampsia","volume":"98","author":"Flint","year":"2019","journal-title":"Acta Obstet. Gynecol. Scand."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Eze, S.C., Ododo, N.A., Ugwu, E.O., Enebe, J.T., Onyegbule, O.A., Eze, I.O., and Ezem, B.U. (2020). Serum selenium levels of pre-eclamptic and normal pregnant women in Nigeria: A comparative study. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0238263"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Abel, M.H., Caspersen, I.H., Sengpiel, V., Jacobsson, B., Meltzer, H.M., Magnus, P., Alexander, J., and Brants\u00e6ter, A.L. (2020). Insufficient maternal iodine intake is associated with subfecundity, reduced foetal growth, and adverse pregnancy outcomes in the Norwegian Mother, Father and Child Cohort Study. BMC Med., 18.","DOI":"10.1186\/s12916-020-01676-w"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Lopez-Jaramillo, P., Barajas, J., Rueda-Quijano, S.M., Lopez-Lopez, C., and Felix, C. (2018). Obesity and Preeclampsia: Common Pathophysiological Mechanisms. Front. Physiol., 9.","DOI":"10.3389\/fphys.2018.01838"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.preghy.2021.09.008","article-title":"The 2021 International Society for the Study of Hypertension in Pregnancy classification, diagnosis & management recommendations for international practice","volume":"27","author":"Magee","year":"2022","journal-title":"Pregnancy Hypertens."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e35","DOI":"10.1161\/HYP.0000000000000087","article-title":"Measurement of Blood Pressure in Humans: A Scientific Statement from the American Heart Association","volume":"73","author":"Muntner","year":"2019","journal-title":"Hypertension"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.preghy.2017.11.006","article-title":"Prediction of adverse maternal outcomes from pre-eclampsia and other hypertensive disorders of pregnancy: A systematic review","volume":"11","author":"Ukah","year":"2018","journal-title":"Pregnancy Hypertens."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1002\/uog.26032","article-title":"Clinical utility of sFlt-1 and PlGF in screening, prediction, diagnosis and monitoring of pre-eclampsia and fetal growth restriction","volume":"61","author":"Stepan","year":"2023","journal-title":"Ultrasound Obstet. Gynecol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"American College of Obstetricians and Gynecologists (2020). ACOG Gestational Hypertension and Preeclampsia: ACOG Practice Bulletin, Number 222. Obstet. Gynecol., 135, e237\u2013e260.","DOI":"10.1097\/AOG.0000000000003891"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"317","DOI":"10.2471\/BLT.07.050112","article-title":"Revisiting Wilson and Jungner in the genomic age: A review of screening criteria over the past 40 years","volume":"86","author":"Andermann","year":"2008","journal-title":"Bull. World Health Organ."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1177\/0004563213517628","article-title":"Contemporary view of the clinical relevance of magnesium homeostasis","volume":"51","author":"Ayuk","year":"2014","journal-title":"Ann. Clin. Biochem."},{"key":"ref_24","unstructured":"Institute of Medicine Standing Committee on the Scientific Evaluation of Dietary Reference Intakes and its Panel on Folate (1997). The National Academies Collection: Reports funded by National Institutes of Health. Dietary Reference Intakes for Calcium, Phosphorus, Magnesium, Vitamin D, and Fluoride, National Academies Press."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1080\/17843286.2018.1516173","article-title":"Hypomagnesemia and hypermagnesemia","volume":"74","year":"2019","journal-title":"Acta Clin. Belg."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3063","DOI":"10.1001\/jama.1990.03440220087036","article-title":"Frequency of hypomagnesemia and hypermagnesemia. Requested vs routine","volume":"263","author":"Whang","year":"1990","journal-title":"JAMA"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1042\/CS20110506","article-title":"Endothelial cells and magnesium: Implications in atherosclerosis","volume":"122","author":"Maier","year":"2012","journal-title":"Clin. Sci."},{"key":"ref_28","first-page":"572","article-title":"Serum electrolyte levels in preeclamptic women: A comparative study","volume":"3","author":"Manjareeka","year":"2012","journal-title":"Int. J. Pharma Bio Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e3151","DOI":"10.31661\/gmj.v12i.3151","article-title":"Serum Calcium and Magnesium Levels in Women Presenting with Pre-eclampsia: A Systematic Review and Meta-analysis Based on Observational Studies","volume":"12","author":"Eslamzadeh","year":"2023","journal-title":"Galen Med. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"108","DOI":"10.4236\/ojog.2020.1010010","article-title":"Serum Magnesium Levels in Second and Third Trimesters of Pregnancy in Patients That Developed Pre-Eclampsia and Feto-Maternal Outcome","volume":"10","author":"Atiba","year":"2020","journal-title":"Open J. Obstet. Gynecol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"561","DOI":"10.4236\/ojog.2014.49080","article-title":"Serum magnesium levels in healthy pregnant and pre-eclamptic patients\u2014A cross-section study","volume":"4","author":"Adekanle","year":"2014","journal-title":"Open J. Obstet. Gynecol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Ephraim, R.K.D., Osakunor, D.N.M., Denkyira, S.W., Eshun, H., Amoah, S., and Anto, E.O. (2014). Serum calcium and magnesium levels in women presenting with pre-eclampsia and pregnancy-induced hypertension: A case-control study in the Cape Coast metropolis, Ghana. BMC Pregnancy Childbirth, 14.","DOI":"10.1186\/s12884-014-0390-2"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1093\/inthealth\/ihad026","article-title":"Association of maternal serum magnesium with pre-eclampsia in African pregnant women: A systematic review and meta-analysis","volume":"16","author":"Tesfa","year":"2023","journal-title":"Int. Health"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Kostov, K., and Halacheva, L. (2018). Role of Magnesium Deficiency in Promoting Atherosclerosis, Endothelial Dysfunction, and Arterial Stiffening as Risk Factors for Hypertension. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19061724"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"AlShanableh, Z., and Ray, E.C. (2024). Magnesium in hypertension: Mechanisms and clinical implications. Front. Physiol., 15.","DOI":"10.3389\/fphys.2024.1363975"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.abb.2006.05.005","article-title":"Role of magnesium in hypertension","volume":"458","author":"Sontia","year":"2007","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1016\/j.bbadis.2010.06.016","article-title":"Magnesium deficiency promotes a pro-atherogenic phenotype in cultured human endothelial cells via activation of NFkB","volume":"1802","author":"Baldoli","year":"2010","journal-title":"Biochim. Biophys. Acta (BBA)\u2014Mol. Basis Dis."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Rodr\u00edguez-Ortiz, M.E., G\u00f3mez-Delgado, F., de Larriva, A.P.A., Canalejo, A., G\u00f3mez-Luna, P., Herencia, C., L\u00f3pez-Moreno, J., Rodr\u00edguez, M., L\u00f3pez-Miranda, J., and Almad\u00e9n, Y. (2019). Serum Magnesium is associated with Carotid Atherosclerosis in patients with high cardiovascular risk (CORDIOPREV Study). Sci. Rep., 9.","DOI":"10.1038\/s41598-019-44322-z"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0098-2997(02)00095-X","article-title":"Low magnesium and atherosclerosis: An evidence-based link","volume":"24","author":"Maier","year":"2003","journal-title":"Mol. Asp. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"118","DOI":"10.3923\/ajcn.2017.118.123","article-title":"Iodine deficiency in pregnancy as a predictor of Sub-clinical hypothyroidism, preeclampsia and future cardiovascular disease","volume":"9","author":"Businge","year":"2017","journal-title":"Asian J. Clin. Nutr."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"661","DOI":"10.20960\/nh.460","article-title":"Iodine levels are associated with oxidative stress and antioxidant status in pregnant women with hypertensive disease","volume":"34","year":"2017","journal-title":"Nutr. Hosp."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"154","DOI":"10.2478\/cipms-2021-0023","article-title":"Urinary iodine as an important indicator for preeclampsia: A Polish perspective","volume":"34","author":"Gajewska","year":"2021","journal-title":"Curr. Issues Pharm. Med. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"744","DOI":"10.3945\/jn.111.149393","article-title":"Global iodine status in 2011 and trends over the past decade","volume":"142","author":"Andersson","year":"2012","journal-title":"J. Nutr."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1089\/thy.2013.0128","article-title":"Global iodine nutrition: Where do we stand in 2013?","volume":"23","author":"Pearce","year":"2013","journal-title":"Thyroid"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.3945\/jn.111.144071","article-title":"Ten repeat collections for urinary iodine from spot samples or 24-hour samples are needed to reliably estimate individual iodine status in women","volume":"141","author":"Andersson","year":"2011","journal-title":"J. Nutr."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"6779094","DOI":"10.1155\/2023\/6779094","article-title":"Variations in the Urinary Iodine Concentration and Urinary Iodine\/Creatinine Ratio among Preschool Children","volume":"2023","author":"An","year":"2023","journal-title":"Int. J. Endocrinol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"WHO Secretariat, Andersson, M., De Benoist, B., Delange, F., and Zupan, J. (2007). Prevention and control of iodine deficiency in pregnant and lactating women and in children less than 2-years-old: Conclusions and recommendations of the Technical Consultation. Public Health Nutr., 10, 1606\u20131611.","DOI":"10.1017\/S1368980007361004"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1038\/sj.ejcn.1600762","article-title":"Day-to-day and within-day variation in urinary iodine excretion","volume":"53","author":"Rasmussen","year":"1999","journal-title":"Eur. J. Clin. Nutr."},{"key":"ref_49","unstructured":"Lazarus, J.H. (2016). Thyroid Regulation and Dysfunction in the Pregnant Patient. Otorhinolaryngology, Head and Neck Surgery, Springer."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s12011-014-9898-6","article-title":"Oxidative stress increased in pregnant women with iodine deficiency","volume":"157","author":"Vidal","year":"2014","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1053\/plac.2000.0560","article-title":"Placental debris, oxidative stress and pre-eclampsia","volume":"21","author":"Redman","year":"2000","journal-title":"Placenta"},{"key":"ref_52","first-page":"548","article-title":"Iodine\u2014A Potential Antioxidant and the Role of Iodine\/Iodide in Health and Disease","volume":"7","author":"Winkler","year":"2015","journal-title":"Nat. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1002\/(SICI)1099-0844(200006)18:2<143::AID-CBF857>3.0.CO;2-#","article-title":"Effect of iodide on total antioxidant status of human serum","volume":"18","author":"Winkler","year":"2000","journal-title":"Cell Biochem. Funct."},{"key":"ref_54","unstructured":"Feingold, K.R., Ahmed, S.F., and Anawalt, B. (2025, March 22). Thyroid Regulation and Dysfunction in the Pregnant Patient, Endotext [Internet], Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK279059\/."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1097\/AOG.0b013e318240de6a","article-title":"Subclinical thyroid disease and the incidence of hypertension in pregnancy","volume":"119","author":"Wilson","year":"2012","journal-title":"Obstet. Gynecol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/s40748-024-00186-4","article-title":"Maternal hypothyroidism and the risk of preeclampsia: A Danish national and regional study","volume":"10","author":"Lundgaard","year":"2024","journal-title":"Matern. Health Neonatol. Perinatol."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Hajifoghaha, M., Teshnizi, S.H., Forouhari, S., and Dabbaghmanesh, M.H. (2022). Association of thyroid function test abnormalities with preeclampsia: A systematic review and meta-analysis. BMC Endocr. Disord., 22.","DOI":"10.1186\/s12902-022-01154-9"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1055\/s-0029-1223285","article-title":"Increased neutrophil numbers account for leukocytosis in women with preeclampsia","volume":"26","author":"Canzoneri","year":"2009","journal-title":"Am. J. Perinatol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.preghy.2020.03.009","article-title":"Predictive role of neutrophil-to-lymphocyte ratio in preeclampsia: A meta-analysis including 3982 patients","volume":"20","author":"Kang","year":"2020","journal-title":"Pregnancy Hypertens."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.1161\/HYPERTENSIONAHA.106.086751","article-title":"Difference in neutrophil superoxide generation during pregnancy between preeclampsia and essential hypertension","volume":"49","author":"Tsukimori","year":"2007","journal-title":"Hypertension"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"491","DOI":"10.33549\/physiolres.930813","article-title":"Oxidized low-density lipoprotein (Oxidized LDL) and the risk of preeclampsia","volume":"55","author":"Qiu","year":"2006","journal-title":"Physiol. Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1186\/s12944-017-0480-z","article-title":"Oxidative profiles of LDL and HDL isolated from women with preeclampsia","volume":"16","author":"Hicks","year":"2017","journal-title":"Lipids Health Dis."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1297658","DOI":"10.1155\/2017\/1297658","article-title":"Selenium and Thyroid Disease: From Pathophysiology to Treatment","volume":"2017","author":"Ventura","year":"2017","journal-title":"Int. J. Endocrinol."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Wang, F., Li, C., Li, S., Cui, L., Zhao, J., and Liao, L. (2023). Selenium and thyroid diseases. Front. Endocrinol., 14.","DOI":"10.3389\/fendo.2023.1133000"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"203.e1\u2013203.e10","DOI":"10.1016\/j.ajog.2012.12.016","article-title":"Performance of a first-trimester screening of preeclampsia in a routine care low-risk setting","volume":"208","author":"Scazzocchio","year":"2013","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1159\/000341712","article-title":"Combined screening for preeclampsia and small for gestational age at 11\u201313 weeks","volume":"33","author":"Poon","year":"2013","journal-title":"Fetal Diagn. Ther."}],"container-title":["Medicina"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1648-9144\/61\/4\/600\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:00:33Z","timestamp":1760029233000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1648-9144\/61\/4\/600"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,26]]},"references-count":66,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["medicina61040600"],"URL":"https:\/\/doi.org\/10.3390\/medicina61040600","relation":{},"ISSN":["1648-9144"],"issn-type":[{"type":"electronic","value":"1648-9144"}],"subject":[],"published":{"date-parts":[[2025,3,26]]}}}