{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:07:54Z","timestamp":1792789674815,"version":"4.5.0"},"reference-count":53,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FEDER\u2014Fundo Europeu de Desenvolimento Regional through the COMPETE 2020\u2014Operational Programme for Competitiveness and Internationalization"},{"name":"Operational Programme for Competitiveness and Internationalization (POCI), Portugal 2020"},{"name":"Portuguese funds through FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","id":[{"id":"https:\/\/ror.org\/00snfqn58","id-type":"ROR","asserted-by":"crossref"}]},{"name":"FCT and FEDER","award":["IF\/00092\/2014\/CP1255\/CT0004"],"award-info":[{"award-number":["IF\/00092\/2014\/CP1255\/CT0004"]}]},{"name":"FCT and FEDER","award":["PRR-09\/C06-834I07\/2024.P11721"],"award-info":[{"award-number":["PRR-09\/C06-834I07\/2024.P11721"]}]},{"name":"FCT and FEDER","award":["2024.18026.PEX"],"award-info":[{"award-number":["2024.18026.PEX"]}]},{"name":"Chair in Onco-Innovation at the FMUP"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objectives: Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are among the most common sexually transmitted infections (STIs), with the highest incidence in individuals aged up to 25 years. However, data from Portugal remain scarce. This cross-sectional pilot study aimed to assess the prevalence of urogenital CT and NG infections in young adults in Portugal and to identify the major CT genotypes circulating in this population. Materials and Methods: A total of 152 young adults aged 18\u201325 years were recruited over a six-month period from universities, a sports club, and a coworking space. Urine samples were tested using the Cobas 4800 CT\/NG assay (Roche, Rotkreuz, Switzerland). CT-positive samples were further genotyped based on ompA gene diversity to identify major urogenital genotypes (D to K). All participants provided informed consent and completed a questionnaire on demographic characteristics and risk behaviors. Results: Of the 152 urine samples analyzed, five tested positive for CT, one for NG, and one sample showed co-infection with both pathogens. None of the participants with positive results reported symptoms. Genotyping of CT-positive samples identified ompA genotype E in three cases. Conclusions: Despite limitations such as small sample size and convenience sampling, this pilot study offers preliminary insights into the prevalence and genetic diversity of CT and NG infections among young adults in Portugal. The findings highlight the need for expanded screening programs, site-specific sample collection, and culture-based diagnostics to support evidence-based public health strategies targeting these infections.<\/jats:p>","DOI":"10.3390\/medicina61101749","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T12:05:13Z","timestamp":1758801913000},"page":"1749","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Community-Based Pilot Study of Chlamydia trachomatis and Neisseria gonorrhoeae Urogenital Infections Among Young Adults in the North and the Central Coast of Portugal"],"prefix":"10.3390","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6525-1551","authenticated-orcid":false,"given":"Rafaela","family":"Rodrigues","sequence":"first","affiliation":[{"name":"PerMed Research Group, Center for Health Technology and Services Research (CINTESIS), Rua Doutor Pl\u00e1cido da Costa, 4200-450 Porto, Portugal"},{"name":"CINTESIS@RISE, Faculty of Medicine, University of Porto, Alameda Professor Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal"},{"name":"Molecular Diagnostics Laboratory, Unilabs Portugal, Centro Empresarial Lionesa Porto, Rua Lionesa, 4465-671 Le\u00e7a do Balio, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"given":"S\u00f3nia","family":"Loureiro","sequence":"additional","affiliation":[{"name":"Molecular Diagnostics Laboratory, Unilabs Portugal, Centro Empresarial Lionesa Porto, Rua Lionesa, 4465-671 Le\u00e7a do Balio, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"In\u00eas","family":"Jo\u00e3o","sequence":"additional","affiliation":[{"name":"National Reference Laboratory (NRL) for Sexually Transmitted Infections (STI), Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge, 1649-016 Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"}]},{"given":"In\u00eas","family":"Jord\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences, University of Aveiro, 3810-193 Aveiro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8604-8800","authenticated-orcid":false,"given":"Maria Jos\u00e9","family":"Borrego","sequence":"additional","affiliation":[{"name":"National Reference Laboratory (NRL) for Sexually Transmitted Infections (STI), Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge, 1649-016 Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Carlos","family":"Catal\u00e3o","sequence":"additional","affiliation":[{"name":"Roche Sistemas de Diagn\u00f3sticos, Estrada Nacional 249-1, 2720-413 Amadora, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Ana Rita","family":"Silva","sequence":"additional","affiliation":[{"name":"Molecular Diagnostics Laboratory, Unilabs Portugal, Centro Empresarial Lionesa Porto, Rua Lionesa, 4465-671 Le\u00e7a do Balio, 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