{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T22:31:47Z","timestamp":1787783507214,"version":"build-2784847793"},"reference-count":38,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Introduction: Sepsis-induced cardiac dysfunction (SICD) is a transient cardiac disfunction, with variable described prevalence and uncertain prognostic. This study aimed to characterize the laboratory and microbiological findings in critically ill patients with sepsis who developed left ventricular (LV) or biventricular systolic dysfunction. Methods: Patients who required intensive care unit hospitalization for sepsis were screened retrospectively. Only patients with positive cultures and echocardiography performed within 24 h from admission were included. The exclusion criteria were infective endocarditis, acute coronary syndrome, history of cardiomyopathy, severe valve disease, end-stage organ or oncological disease. Cardiac function was appreciated on transthoracic echocardiography, using LV ejection fraction for the left ventricle and tricuspid annular plane systolic excursion (TAPSE) for the right ventricle. SICD was confirmed if the systolic dysfunction found upon admission was reversible within 7\u201310 days. Results: A total of 100 patients with positive cultures were included. The median age was 73 and 55% were male. SICD was diagnosed in 14% of patients. Patients with SICD were more likely to develop septic shock and had longer hospital and intensive care unit stay. In-hospital mortality was 44% with no significant difference between SICD and non-SICD patients. Laboratory markers upon hospital admission showed that SICD patients had significantly higher values of lactate and transaminases. Cardiac (troponin and NT-proBNP) and inflammation markers (leukocytes, neutrophils, NLR, C-reactive protein, procalcitonin) had higher values in patients with SICD but the difference did not reach statistical significance. Streptococcal infections and polymicrobial cultures were risk factors for developing SICD. Higher rates of infections with Enterobacterales were seen in the SICD group but the difference was not significant. Conclusions: SICD patients had higher lactate, inflammation, and cardiac biomarkers levels upon admission and significantly higher rates of streptococcal infections and polymicrobial cultures.<\/jats:p>","DOI":"10.3390\/medicina61101765","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T09:12:23Z","timestamp":1759223543000},"page":"1765","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Laboratory and Microbiological Considerations in Sepsis-Induced Cardiac Dysfunction"],"prefix":"10.3390","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4951-2221","authenticated-orcid":false,"given":"Catalina","family":"Paraschiv","sequence":"first","affiliation":[{"name":"Department of Cardiothoracic Pathology, University of Medicine and Pharmacy \u201cCarol Davila\u201d, 011461 Bucharest, Romania"},{"name":"Cardiology Unit, Elias Emergency University Hospital, University of Medicine and Pharmacy \u201cCarol Davila\u201d, 011461 Bucharest, Romania"},{"name":"Department of Cardiovascular Diseases, Mayo Clinic, Rochester, MN 55905, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denisa Oana","family":"Nicolaescu","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Pathology, University of Medicine and Pharmacy \u201cCarol Davila\u201d, 011461 Bucharest, Romania"},{"name":"Cardiology Unit, Elias Emergency University Hospital, University of Medicine and Pharmacy \u201cCarol Davila\u201d, 011461 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihaela Roxana","family":"Popescu","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Pathology, University of Medicine and Pharmacy \u201cCarol Davila\u201d, 011461 Bucharest, Romania"},{"name":"Cardiology Unit, Elias Emergency University Hospital, University of Medicine and Pharmacy \u201cCarol Davila\u201d, 011461 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9635-1177","authenticated-orcid":false,"given":"Carmen Cristina","family":"Vasile","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, National Institute for Infectious Diseases \u201cProf. Dr. Matei Bals\u201d, University of Medicine and Pharmacy \u201cCarol Davila\u201d, 021105 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuel","family":"Moisa","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia and Intensive Care Medicine, Elias Emergency University Hospital, University of Medicine and Pharmacy \u201cCarol Davila\u201d, 011461 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9384-6870","authenticated-orcid":false,"given":"Silvius Ioan","family":"Negoita","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia and Intensive Care Medicine, Elias Emergency University Hospital, University of Medicine and Pharmacy \u201cCarol Davila\u201d, 011461 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4949-3064","authenticated-orcid":false,"given":"Serban Mihai","family":"Balanescu","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Pathology, University of Medicine and Pharmacy \u201cCarol Davila\u201d, 011461 Bucharest, Romania"},{"name":"Cardiology Unit, Elias Emergency University Hospital, University of Medicine and Pharmacy \u201cCarol Davila\u201d, 011461 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1001\/jama.2016.0287","article-title":"The third international consensus definitions for sepsis and septic shock (sepsis-3)","volume":"315","author":"Singer","year":"2016","journal-title":"JAMA\u2014J. Am. Med. Assoc."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/S0140-6736(19)32989-7","article-title":"Global, regional, and national sepsis incidence and mortality, 1990\u20132017: Analysis for the Global Burden of Disease Study","volume":"395","author":"Rudd","year":"2020","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1552","DOI":"10.1007\/s00134-020-06151-x","article-title":"Incidence and mortality of hospital- and ICU-treated sepsis: Results from an updated and expanded systematic review and meta-analysis","volume":"46","author":"Mellhammar","year":"2020","journal-title":"Intensive Care Med."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Ljungstr\u00f6m, L., Andersson, R., and Jacobsson, G. (2019). Incidences of community onset severe sepsis, Sepsis-3 sepsis, and bacteremia in Sweden\u2014A prospective population-based study. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0225700"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2323","DOI":"10.1001\/jama.2009.1754","article-title":"International study of the prevalence and outcomes of infection in intensive care units","volume":"302","author":"Vincent","year":"2009","journal-title":"JAMA"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2495","DOI":"10.1001\/jama.2010.851","article-title":"Infection rate and acute organ dysfunction risk as explanations for racial differences in severe sepsis","volume":"303","author":"Mayr","year":"2010","journal-title":"JAMA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1186\/s13054-019-2431-8","article-title":"An overview of positive cultures and clinical outcomes in septic patients: A sub-analysis of the Prehospital Antibiotics Against Sepsis (PHANTASi) trial","volume":"23","author":"Panday","year":"2019","journal-title":"Crit. Care"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1016\/j.chest.2016.08.1460","article-title":"Culture-Negative Severe Sepsis: Nationwide Trends and Outcomes","volume":"150","author":"Gupta","year":"2016","journal-title":"Chest"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.1097\/CCM.0000000000005870","article-title":"Epidemiology, Resistance Profiles, and Outcomes of Bloodstream Infections in Community-Onset Sepsis in the United States","volume":"51","author":"Ohnuma","year":"2023","journal-title":"Crit. Care Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.chest.2018.08.1037","article-title":"The Septic Heart: Current Understanding of Molecular Mechanisms and Clinical Implications","volume":"155","author":"Martin","year":"2019","journal-title":"Chest"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1177\/08850666231180526","article-title":"Prevalence and Prognosis of Sepsis-Induced Cardiomyopathy: A Systematic Review and Meta-Analysis","volume":"38","author":"Hasegawa","year":"2023","journal-title":"J. Intensive Care Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"155250","DOI":"10.1016\/j.jcrc.2025.155250","article-title":"Current challenges in understanding, diagnosing and managing sepsis-induced cardiac dysfunction","volume":"91","author":"Paraschiv","year":"2026","journal-title":"J. Crit. Care"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Liu, Y.-C., Yu, M.-M., Shou, S.-T., and Chai, Y.-F. (2017). Sepsis-induced cardiomyopathy: Mechanisms and treatments. Front. Immunol., 8.","DOI":"10.3389\/fimmu.2017.01021"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1093\/infdis\/jiaa371","article-title":"Cardiac microlesions form during severe bacteremic enterococcus faecalis infection","volume":"223","author":"Brown","year":"2021","journal-title":"J. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Svennerholm, K., Park, K.-S., Wikstr\u00f6m, J., L\u00e4sser, C., Crescitelli, R., Shelke, G.V., Jang, S.C., Suzuki, S., Bandeira, E., and Olofsson, C.S. (2017). Escherichia coli outer membrane vesicles can contribute to sepsis induced cardiac dysfunction. Sci. Rep., 7.","DOI":"10.1038\/s41598-017-16363-9"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.cyto.2012.03.023","article-title":"Cardiac dysfunction in StrepTSS: Group A streptococcus disrupts the directional cardiomyocyte-to-macrophage crosstalk that maintains macrophage quiescence","volume":"59","author":"Li","year":"2012","journal-title":"Cytokine"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1097\/SHK.0000000000000266","article-title":"Cardiac myocyte dysfunction induced by streptolysin O is membrane pore and calcium dependent","volume":"43","author":"Bolz","year":"2015","journal-title":"Shock"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e5031","DOI":"10.1097\/MD.0000000000005031","article-title":"Prevalence and risk factors of sepsis-induced cardiomyopathy A retrospective cohort study","volume":"95","author":"Sato","year":"2016","journal-title":"Medicine"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e0028322","DOI":"10.1128\/iai.00283-22","article-title":"Sex and Gender Differences in Bacterial Infections","volume":"90","author":"Dias","year":"2022","journal-title":"Infect. Immun."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1177\/0885066612471621","article-title":"Plasma brain natriuretic peptide and troponin levels in severe sepsis and septic shock: Relationships with systolic myocardial dysfunction and intensive care unit mortality","volume":"29","author":"Klouche","year":"2014","journal-title":"J. Intensive Care Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"9452","DOI":"10.12998\/wjcc.v9.i31.9452","article-title":"Incidence, prognosis, and risk factors of sepsis-induced cardiomyopathy","volume":"9","author":"Liang","year":"2021","journal-title":"World J. Clin. Cases"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1097\/CCM.0000000000000742","article-title":"Lactate measurements in sepsis-induced tissue hypoperfusion: Results from the surviving sepsis campaign database","volume":"43","author":"Casserly","year":"2015","journal-title":"Crit. Care Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1093\/eurheartj\/ehr351","article-title":"Diastolic dysfunction and mortality in severe sepsis and septic shock","volume":"33","author":"Landesberg","year":"2012","journal-title":"Eur. Heart J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e1014","DOI":"10.1002\/iid3.1014","article-title":"Cardiac troponin as a prognosticator of mortality in patients with sepsis: A systematic review and meta-analysis","volume":"11","author":"Zheng","year":"2023","journal-title":"Immun. Inflamm. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/s13054-025-05249-2","article-title":"Early high-sensitivity troponin elevation and short-term mortality in sepsis: A systematic review with meta-analysis","volume":"29","author":"Gajardo","year":"2025","journal-title":"Crit. Care"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.mayocpiqo.2019.10.008","article-title":"Natriuretic Peptides to Predict Short-Term Mortality in Patients with Sepsis: A Systematic Review and Meta-analysis","volume":"4","author":"Wang","year":"2020","journal-title":"Mayo Clin. Proc. Innov. Qual. Outcomes"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1378\/chest.14-2259","article-title":"Myocardial dysfunction in severe sepsis and septic shock: No correlation with inflammatory cytokines in real-life clinical setting","volume":"148","author":"Landesberg","year":"2015","journal-title":"Chest"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"215","DOI":"10.4266\/acc.2021.00234","article-title":"Sepsis-induced cardiomyopathy is associated with higher mortality rates in patients with sepsis","volume":"36","author":"Hanumanthu","year":"2021","journal-title":"Acute Crit. Care"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S1473-3099(09)70066-0","article-title":"Gram-positive toxic shock syndromes","volume":"9","author":"Lappin","year":"2009","journal-title":"Lancet Infect. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Ruiz-Rodr\u00edguez, J.C., Chiscano-Cam\u00f3n, L., Maldonado, C., Ruiz-Sanmartin, A., Martin, L., Baja\u00f1a, I., Bastidas, J., Lopez-Martinez, R., Franco-Jarava, C., and Gonz\u00e1lez-L\u00f3pez, J.J. (2024). Catastrophic Streptococcus pyogenes Disease: A Personalized Approach Based on Phenotypes and Treatable Traits. Antibiotics, 13.","DOI":"10.3390\/antibiotics13020187"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Alhamdi, Y., Neill, D.R., Abrams, S.T., Malak, H.A., Yahya, R., Barrett-Jolley, R., Wang, G., Kadioglu, A., and Toh, C.-H. (2015). Circulating Pneumolysin Is a Potent Inducer of Cardiac Injury during Pneumococcal Infection. PLoS Pathog., 11.","DOI":"10.1371\/journal.ppat.1004836"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"H2316","DOI":"10.1152\/ajpheart.00763.2001","article-title":"Escherichia coli LPS-induced LV dysfunction: Role of toll-like receptor-4 in the adult heart","volume":"282","author":"Nemoto","year":"2002","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1056\/NEJM198908033210503","article-title":"The Cardiovascular Response of Normal Humans to the Administration of Endotoxin","volume":"321","author":"Suffredini","year":"1989","journal-title":"New Engl. J. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1002\/iju5.12540","article-title":"A case of sepsis-induced cardiomyopathy successfully treated with venoarterial extracorporeal membrane oxygenation","volume":"6","author":"Sato","year":"2023","journal-title":"IJU Case Rep."},{"key":"ref_35","first-page":"e76552","article-title":"Sepsis-Induced Cardiomyopathy Secondary to Escherichia coli Sepsis and Intestinal Perforation: A Diagnostic Challenge in a Paediatric Patient","volume":"16","author":"Kumar","year":"2024","journal-title":"Cureus"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1016\/j.molimm.2013.07.007","article-title":"Lipoteichoic acid from Staphylococcus aureus directly affects cardiomyocyte contractility and calcium transients","volume":"56","author":"Mutig","year":"2013","journal-title":"Mol. Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3693","DOI":"10.1161\/01.CIR.0000143081.13042.04","article-title":"Toll-like receptor 2 mediates Staphylococcus aureus-induced myocardial dysfunction and cytokine production in the heart","volume":"110","author":"Knuefermann","year":"2004","journal-title":"Circulation"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1161\/CIRCULATIONAHA.104.503938","article-title":"Mechanisms of cardiac depression caused by lipoteichoic acids from Staphylococcus aureus in isolated rat hearts","volume":"112","author":"Grandel","year":"2005","journal-title":"Circulation"}],"container-title":["Medicina"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1648-9144\/61\/10\/1765\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T11:32:23Z","timestamp":1766489543000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1648-9144\/61\/10\/1765"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,30]]},"references-count":38,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,10]]}},"alternative-id":["medicina61101765"],"URL":"https:\/\/doi.org\/10.3390\/medicina61101765","relation":{},"ISSN":["1648-9144"],"issn-type":[{"value":"1648-9144","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,30]]}}}