{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T12:05:19Z","timestamp":1767873919140,"version":"3.49.0"},"reference-count":43,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T00:00:00Z","timestamp":1767484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Financial support Centro Ciencia & Vida","award":["FB210008"],"award-info":[{"award-number":["FB210008"]}]},{"name":"Financiamiento Basal para Centros Cient\u00edficos y Tecnol\u00f3gicos de Excelencia de ANID"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objectives: Non-coding RNAs (ncRNAs) are genetic transcripts that do not produce proteins but are increasingly recognised for their roles in cellular processes and disease. Specifically, ncRNAs are implicated in the landscape activation of molecular triggers for different diseases, including cancer and viral infections. The function of Sense non-coding mitochondrial RNA-2 (SncmtRNA-2) is currently unknown. This study aims to investigate the roles of SncmtRNA-2 and hsa-miR-620 in Ras-induced cellular transformation. Materials and Methods: The study utilized isoforms V, K, and H of the Ras oncogene and analysed the expression of SncmtRNA-2 and hsa-miR-620 in response to Ras activity. Additionally, both in silico and in vitro analyses were performed to assess whether PML mRNA is a putative target of hsa-miR-620 although direct binding to the PML 3\u2032UTR was not experimentally tested. Results: The research demonstrated that transformation induced by Ras isoforms V, K, and H resulted in decreased expression of both SncmtRNA-2 and hsa-miR-620. Further investigation revealed that hsa-miR-620 is produced by the processing of SncmtRNA-2. It was also shown that Ras increases the expression of Promyelocytic Leukemia Protein (PML). In silico prediction combined with miR-620 gain and loss of function experiments supports PML as a putative hsa-miR-620 target. Conclusions: Ras promotes cellular transformation by decreasing the expression of SncmtRNA-2 and hsa-miR-620, which may contribute to increased PML expression, suggesting but not demonstrating a possible regulatory relationship among these molecules in HPV-immortalised cells. These results highlight a potential SncmtRNA-2\/miR-620\/PML axis that requires further validation through direct interaction assays and functional necessity\/sufficiency experiments.<\/jats:p>","DOI":"10.3390\/medicina62010110","type":"journal-article","created":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T08:40:53Z","timestamp":1767602453000},"page":"110","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["HPV-18-Immortalised Cells Require the Downregulation of the SncmtRNA-2\/Hsa-miR-620 Axis During Cell Transformation"],"prefix":"10.3390","volume":"62","author":[{"given":"Emanuel","family":"Jeldes","sequence":"first","affiliation":[{"name":"Centro Cient\u00edfico y Tecnol\u00f3gico de Excelencia Ciencia y Vida, Santiago 8340148, Chile"},{"name":"Centre for Inflammation Research, Institute for Regeneration and Repair, University of Edinburgh, Edinburgh EH166 4UU, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5857-4793","authenticated-orcid":false,"given":"Manuel","family":"Varas-Godoy","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Celular y Biomedicina (CEBICEM), Facultad de Ciencias, Universidad San Sebasti\u00e1n, Santiago 8420524, Chile"},{"name":"Centro Ciencia & Vida, Fundaci\u00f3n Ciencia & Vida, Santiago 8320000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulina","family":"Gonz\u00e1lez-Chac\u00f3n","sequence":"additional","affiliation":[{"name":"Escuela de bioqu\u00edmica, Facultad de Ciencias de la Vida, Universidad Andr\u00e9s Bello, Santiago 8370000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Am\u00e9rica V.","family":"Campos","sequence":"additional","affiliation":[{"name":"Cancer Research UK Scotland Institute, Glasgow G61 1BD, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6147-3325","authenticated-orcid":false,"given":"Alberto J. M.","family":"Mart\u00edn","sequence":"additional","affiliation":[{"name":"Escuela de Ingenier\u00eda, Facultad de Ingeniera, Arquitectura y Dise\u00f1o, Universidad San Sebasti\u00e1n, Santiago 8420524, Chile"},{"name":"Centers of Research Excellence in Science and Technology, Science & Life, Santiago 8580702, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camilo","family":"Villaman","sequence":"additional","affiliation":[{"name":"Centers of Research Excellence in Science and Technology, Science & Life, Santiago 8580702, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1ngel","family":"Roco-Videla","sequence":"additional","affiliation":[{"name":"Facultad de Ingenier\u00eda, Universidad Cat\u00f3lica de la Sant\u00edsima Concepci\u00f3n, Concepci\u00f3n 4090541, Chile"},{"name":"Direcci\u00f3n de Desarrollo y Postgrados, Universidad Aut\u00f3noma de Chile, Galvarino Gallardo 1983, Santiago 7500138, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9556-4655","authenticated-orcid":false,"given":"Jaime","family":"Villegas Olavarr\u00eda","sequence":"additional","affiliation":[{"name":"Escuela de Medicina Veterinaria, Faculty de Ciencias de la Vida, Universidad Andr\u00e9s Bello, Santiago 8370000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6233-8563","authenticated-orcid":false,"given":"Claudio","family":"Villota Arcos","sequence":"additional","affiliation":[{"name":"Escuela de Nutrici\u00f3n y Diet\u00e9tica, Facultad de Ciencias de la Salud, Universidad Bernardo O\u2019Higgins, Santiago 8370993, Chile"},{"name":"Escuela de Medicina, Facultad de Ciencias M\u00e9dicas, Universidad Bernardo O\u2019Higgins, Santiago 8370993, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.radonc.2013.06.004","article-title":"Human papillomaviruses and cancer","volume":"108","author":"Haedicke","year":"2013","journal-title":"Radiother. 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