{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T02:27:54Z","timestamp":1769480874338,"version":"3.49.0"},"reference-count":33,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T00:00:00Z","timestamp":1769385600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objectives: Endoscopic biopsy of brain lesions plays an important role in the management of intra- and periventricular lesions. While the diagnostic yield of this technique has been reported with varying success across studies, its outcome is likely influenced by specific technical nuances of the procedure. However, the relationship between these technical factors and diagnostic accuracy remains understudied in the current literature. We aim to describe the procedural rationale, key anatomical considerations, and technical nuances of the endoscopic biopsy of intra- and paraventricular brain lesions, comparing standard tissue forceps with a side-cutting biopsy needle technique. Materials and Methods: We conducted a ten-year single-center, retrospective study of patients who underwent endoscopic biopsy for intra- and paraventricular brain lesions between January 2014 and December 2024. Patients were divided based on the biopsy technique used: the first group of 11 patients was treated using a side-cutting biopsy needle from the center of the lesion, while the second group of five patients underwent tissue sampling with standard endoscopic tissue cup forceps. The study evaluates and compares both approaches in terms of safety and diagnostic accuracy. Results: Endoscopic visualization enabled direct assessment of the biopsy site in both groups. Histopathological diagnoses were successfully obtained in all cases with a side-cutting biopsy needle (11\/11, 100.0%), and in almost all cases with the cup forceps technique (4\/5, 80.0%). In patients with obstructive hydrocephalus, an endoscopic third ventriculostomy (ETV) was performed as the first and therapeutic step in all procedures and two patients required a shunt procedure. Conclusions: Endoscopic biopsies utilizing a side-cutting biopsy needle strategy offer a promising adjunctive approach for selected intra- and paraventricular brain lesions. This method allows for direct visualization of the intraventricular surface, while the use of a needle biopsy can enhance the likelihood of obtaining diagnostically representative tissue with a high degree of reliability.<\/jats:p>","DOI":"10.3390\/medicina62020260","type":"journal-article","created":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T15:48:30Z","timestamp":1769442510000},"page":"260","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Endoscopic Biopsy of Intra- and Paraventricular Brain Lesions: Practical Advantages and Clinical Experience"],"prefix":"10.3390","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3243-3196","authenticated-orcid":false,"given":"Bojan","family":"Jelaca","sequence":"first","affiliation":[{"name":"Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia"},{"name":"Clinic of Neurosurgery, University Clinical Center of Vojvodina, 21000 Novi Sad, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nebojsa","family":"Lasica","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia"},{"name":"Clinic of Neurosurgery, University Clinical Center of Vojvodina, 21000 Novi Sad, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2100-4316","authenticated-orcid":false,"given":"Milica","family":"Gledja","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia"},{"name":"Clinic of Neurosurgery, University Clinical Center of Vojvodina, 21000 Novi Sad, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1875-3592","authenticated-orcid":false,"given":"Veljko","family":"Pantelic","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia"},{"name":"Clinic of Neurosurgery, University Clinical Center of Vojvodina, 21000 Novi Sad, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5524-246X","authenticated-orcid":false,"given":"Jagos","family":"Golubovic","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia"},{"name":"Clinic of Neurosurgery, University Clinical Center of Vojvodina, 21000 Novi Sad, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3131-7855","authenticated-orcid":false,"given":"Djula","family":"Djilvesi","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia"},{"name":"Clinic of Neurosurgery, University Clinical Center of Vojvodina, 21000 Novi Sad, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1227\/00006123-197803000-00006","article-title":"Endoscopic biopsy of intraventricular tumors with the use of a ventriculofiberscope","volume":"2","author":"Fukushima","year":"1978","journal-title":"Neurosurgery"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1227\/01.neu.0000316262.74843.dd","article-title":"Application of neuroendoscopy to intraventricular lesions","volume":"62","author":"Cappabianca","year":"2008","journal-title":"Neurosurgery"},{"key":"ref_3","first-page":"286","article-title":"Endoscopic surgery of third ventricle lesions","volume":"46","author":"Decq","year":"2000","journal-title":"Neurochirurgie"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.wneu.2015.09.011","article-title":"Diagnostic yield, morbidity, and mortality of intraventricular neuroendoscopic biopsy: Systematic review and meta-analysis","volume":"85","author":"Somji","year":"2016","journal-title":"World Neurosurg."},{"key":"ref_5","first-page":"107","article-title":"Endoscopic biopsy of intra- and paraventricular brain tumors","volume":"14","author":"Stachura","year":"2019","journal-title":"Wideochir. Inne Tech. Maloinwazyjne"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/S1042-3680(03)00060-3","article-title":"Epidemiology and pathology of intraventricular tumors","volume":"14","author":"Waldron","year":"2003","journal-title":"Neurosurg. Clin. N. Am."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2367","DOI":"10.1007\/s00381-022-05654-w","article-title":"Endoscopic biopsy of pineal tumors: Two burr hole trans-foramen of Monro approach and endonasal trans-tuber cinereum approach","volume":"39","author":"Kim","year":"2023","journal-title":"Childs Nerv. Syst."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1055\/s-0035-1549304","article-title":"Endoscopic approaches to intraventricular lesions","volume":"76","author":"Russo","year":"2015","journal-title":"J. Neurol. Surg. A Cent. Eur. Neurosurg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"E4","DOI":"10.3171\/2011.1.FOCUS10295","article-title":"Efficacy of simultaneous single-trajectory endoscopic tumor biopsy and endoscopic cerebrospinal fluid diversion procedures in intra- and paraventricular tumors","volume":"30","author":"Mohanty","year":"2011","journal-title":"Neurosurg. Focus"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"34","DOI":"10.3171\/2014.9.JNS132648","article-title":"Neuroendoscopic biopsy of brain lesions: Accuracy and complications","volume":"122","author":"Giannetti","year":"2015","journal-title":"J. Neurosurg."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1007\/s00701-005-0495-5","article-title":"Haemorrhagic complications and the incidence of asymptomatic bleeding associated with stereotactic brain biopsies","volume":"147","author":"Grossman","year":"2005","journal-title":"Acta Neurochir."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/s00701-007-1473-x","article-title":"Safety and efficacy of frameless and frame-based intracranial biopsy techniques","volume":"150","author":"Dammers","year":"2008","journal-title":"Acta Neurochir."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e208","DOI":"10.1016\/j.wneu.2019.02.012","article-title":"Visual-controlled endoscopic biopsy of paraventricular intraparenchymal tumors","volume":"126","author":"Oertel","year":"2019","journal-title":"World Neurosurg."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"704","DOI":"10.3171\/2013.3.PEDS12416","article-title":"Safety and diagnostic accuracy of neuroendoscopic biopsies: An international multicenter study","volume":"11","author":"Constantini","year":"2013","journal-title":"J. Neurosurg. Pediatr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/j.wneu.2015.10.041","article-title":"Intraoperative hemorrhage in ventriculoscopic surgery: Experience of a single Chinese neurosurgery center","volume":"88","author":"Li","year":"2016","journal-title":"World Neurosurg."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1080\/0268869021000030276","article-title":"The role of neuroendoscopy in the management of brain tumours","volume":"16","author":"Macarthur","year":"2002","journal-title":"Br. J. Neurosurg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1007\/s00701-014-1995-y","article-title":"Narrow band imaging-guided endoscopic biopsy for intraventricular and paraventricular brain tumors: Clinical experience with 14 cases","volume":"156","author":"Sasagawa","year":"2014","journal-title":"Acta Neurochir."},{"key":"ref_18","first-page":"171369","article-title":"From the idea to its realization: The evolution of minimally invasive techniques in neurosurgery","volume":"2013","author":"Grunert","year":"2013","journal-title":"Minim. Invasive Surg."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"S14","DOI":"10.1016\/j.wneu.2012.02.032","article-title":"Instrumentation: Endoscopes and equipment","volume":"79","author":"Gaab","year":"2013","journal-title":"World Neurosurg."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1080\/01616412.1999.11740895","article-title":"Application of new electrosurgical devices and probes in endoscopic neurosurgery","volume":"21","author":"Hellwig","year":"1999","journal-title":"Neurol. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"897","DOI":"10.3171\/jns.2005.102.5.0897","article-title":"Independent predictors of morbidity after image-guided stereotactic brain biopsy: A risk assessment of 270 cases","volume":"102","author":"McGirt","year":"2005","journal-title":"J. Neurosurg."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1002\/(SICI)1097-0142(19980501)82:9<1756::AID-CNCR23>3.0.CO;2-2","article-title":"The safety and efficacy of stereotactic biopsy for intracranial lesions","volume":"82","author":"Wa","year":"1998","journal-title":"Cancer"},{"key":"ref_23","first-page":"419","article-title":"Neuroendoscopic biopsy: Experience in 31 patients and literature review","volume":"22","year":"2011","journal-title":"Neurocirug\u00eda"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.3171\/2011.7.JNS101976","article-title":"Nationwide investigation of the current status of therapeutic neuroendoscopy for ventricular and paraventricular tumors in Japan","volume":"115","author":"Hayashi","year":"2011","journal-title":"J. Neurosurg."},{"key":"ref_25","first-page":"177","article-title":"Hemorrhage after stereotactic biopsy from intra-axial brain lesions: Incidence and avoidance","volume":"75","author":"Shakal","year":"2014","journal-title":"J. Neurol. Surg. A Cent. Eur. Neurosurg."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1159\/000492057","article-title":"Safe stereotactic biopsy for basal ganglia lesions: Avoiding injury to the basal perforating arteries","volume":"96","author":"Sato","year":"2018","journal-title":"Stereotact. Funct. Neurosurg."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1007\/s10143-025-03471-8","article-title":"The impact of neuroendoscopic drainage in intraventricular hemorrhage: An updated meta-analysis","volume":"48","author":"Lindner","year":"2025","journal-title":"Neurosurg. Rev."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"E8","DOI":"10.3171\/foc.2005.19.6.9","article-title":"Endoscopic third ventriculostomy for tumor-related hydrocephalus in a pediatric population","volume":"19","author":"Ray","year":"2005","journal-title":"Neurosurg. Focus"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1055\/s-0043-1774308","article-title":"Endoscopic third ventriculostomy versus ventriculoperitoneal shunt in patients with obstructive hydrocephalus: An updated systematic review and meta-analysis","volume":"18","author":"Pasqualotto","year":"2023","journal-title":"Asian J. Neurosurg."},{"key":"ref_30","first-page":"e25136","article-title":"Failure of endoscopic third ventriculostomy","volume":"14","author":"Lane","year":"2022","journal-title":"Cureus"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"399","DOI":"10.3171\/2014.10.PEDS14250","article-title":"Reopening of an obstructed third ventriculostomy: Long-term success and factors affecting outcome in 215 infants","volume":"15","author":"Marano","year":"2015","journal-title":"J. Neurosurg. Pediatr."},{"key":"ref_32","first-page":"43","article-title":"Mechanisms of failure after endoscopic third ventriculostomy in young infants","volume":"103","author":"Wagner","year":"2005","journal-title":"J. Neurosurg."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1007\/s00381-011-1405-1","article-title":"Single burr hole endoscopic biopsy with third ventriculostomy: Measurements and computer-assisted planning","volume":"27","author":"Knaus","year":"2011","journal-title":"Childs Nerv. 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