{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T11:06:15Z","timestamp":1779879975297,"version":"3.53.1"},"reference-count":34,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objectives: This study evaluated the effect of two wound closure methods\u2014polypropylene sutures and a butyl-2-cyanoacrylate tissue adhesive\u2014on the rate of soft tissue regeneration following palatal donor site harvesting. A bovine collagen sponge, used as a secondary-intention dressing, was evaluated descriptively. Materials and Methods: Data from 300 patients (n = 100\/group) with palatal donor sites were analyzed. Primary analysis compared suture vs. adhesive using Early Wound Healing Score (EHS) at days 7 and 14. Secondary outcomes were granulation tissue (day 7) and complications. Statistical methods: Mann\u2013Whitney U test for between-group comparison (suture vs. adhesive); Kruskal\u2013Wallis with Dunn\u2019s post hoc for granulation across all three groups; Spearman\u2019s correlation and logistic regression for the relationship between granulation tissue and EHS within primary healing groups. Results: At day 7, median EHS was similar between suture and adhesive groups (7.0 [interquartile range (IQR) 5.0\u20139.0] vs. 7.0 [IQR 7.0\u20139.0]; p = 0.31). By day 14, both groups achieved excellent healing (median 10.0, IQR 9.0\u201310.0 in both; p = 0.82). The collagen sponge group showed slower healing (median EHS day 7 = 4.0 [IQR 3.0\u20135.0], day 14 = 6.0 [IQR 5.0\u20137.0]), reported descriptively as expected for secondary intention. Granulation tissue on day 7 was highest in the adhesive group (p &lt; 0.001 vs. collagen; p = 0.024 vs. suture). A strong positive correlation between day-7 granulation tissue and day-14 EHS was found in the primary-healing groups (\u03c1 = 0.78, p &lt; 0.001). Receiver operating characteristic (ROC) analysis established a granulation score \u2265 2 as the optimal cut-off for predicting successful healing (EHS \u2265 9) by day 14 (sensitivity 89.4%, specificity 76.0%, area under the curve (AUC) = 0.80), pending external validation. Conclusions: Surgical adhesive may be considered a viable alternative to sutures for palatal donor sites closed by primary intention, offering comparable healing by day 14. Collagen sponges result in slower healing and should be considered only when secondary intention is specifically desired. Early assessment of granulation tissue may serve as a simple prognostic indicator, but external validation is needed before clinical application.<\/jats:p>","DOI":"10.3390\/medicina62050997","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T10:19:52Z","timestamp":1779877192000},"page":"997","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Evaluation of Soft Tissue Regeneration Rate Using Different Wound Closure Methods After Palatal Donor Site Harvesting: A Retrospective Cohort Study"],"prefix":"10.3390","volume":"62","author":[{"given":"Timofei","family":"Ryko","sequence":"first","affiliation":[{"name":"Information Technologies, Mechanics and Optics University\u2014ITMO University, 9 Lomonosov St., Saint Petersburg 191002, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7685-1800","authenticated-orcid":false,"given":"Anton","family":"Timoshin","sequence":"additional","affiliation":[{"name":"Borovskiy Institute of Dentistry, FSAEI HE I.M. Sechenov First MSMU of MOH of Russia\u2014Sechenovskiy University, 8\/2 Trubetskaya St., Moscow 119991, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5298-5767","authenticated-orcid":false,"given":"Alla","family":"Shakaryants","sequence":"additional","affiliation":[{"name":"Borovskiy Institute of Dentistry, FSAEI HE I.M. Sechenov First MSMU of MOH of Russia\u2014Sechenovskiy University, 8\/2 Trubetskaya St., Moscow 119991, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6233-0775","authenticated-orcid":false,"given":"Vitaly","family":"Borisov","sequence":"additional","affiliation":[{"name":"Borovskiy Institute of Dentistry, FSAEI HE I.M. Sechenov First MSMU of MOH of Russia\u2014Sechenovskiy University, 8\/2 Trubetskaya St., Moscow 119991, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4547-2821","authenticated-orcid":false,"given":"Kirill","family":"Ershov","sequence":"additional","affiliation":[{"name":"Borovskiy Institute of Dentistry, FSAEI HE I.M. Sechenov First MSMU of MOH of Russia\u2014Sechenovskiy University, 8\/2 Trubetskaya St., Moscow 119991, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3673-3209","authenticated-orcid":false,"given":"Maria","family":"Timoshina","sequence":"additional","affiliation":[{"name":"Borovskiy Institute of Dentistry, FSAEI HE I.M. Sechenov First MSMU of MOH of Russia\u2014Sechenovskiy University, 8\/2 Trubetskaya St., Moscow 119991, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3963-488X","authenticated-orcid":false,"given":"Elena","family":"Emelina","sequence":"additional","affiliation":[{"name":"Borovskiy Institute of Dentistry, FSAEI HE I.M. Sechenov First MSMU of MOH of Russia\u2014Sechenovskiy University, 8\/2 Trubetskaya St., Moscow 119991, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6481-6017","authenticated-orcid":false,"given":"Aglaya","family":"Kazumova","sequence":"additional","affiliation":[{"name":"Borovskiy Institute of Dentistry, FSAEI HE I.M. Sechenov First MSMU of MOH of Russia\u2014Sechenovskiy University, 8\/2 Trubetskaya St., Moscow 119991, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1111\/prd.12415","article-title":"Management and prevention of soft tissue complications in implant dentistry","volume":"88","author":"Thoma","year":"2022","journal-title":"Periodontology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1111\/prd.12466","article-title":"Wound healing dynamics, morbidity, and complications of palatal soft-tissue harvesting","volume":"92","author":"Tavelli","year":"2023","journal-title":"Periodontology"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Chong, W.J., Razali, M., Baharin, B., and Nik Azis, N.M. (2025). Postoperative pain and morbidity at the donor site after palatal soft tissue harvesting: A systematic review. BMC Oral Health, 25.","DOI":"10.1186\/s12903-025-07198-2"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.ajoms.2020.11.007","article-title":"Topical hemostatic agents from an oral-surgery perspective","volume":"33","author":"Ruangchainicom","year":"2021","journal-title":"J. Oral Maxillofac. Surg. Med. Pathol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e1-21","DOI":"10.1097\/TA.0000000000002508","article-title":"A comprehensive review of topical hemostatic agents: The good, the bad, and the novel","volume":"88","author":"Tompeck","year":"2020","journal-title":"J. Trauma Acute Care Surg."},{"key":"ref_6","first-page":"96","article-title":"Various suturing material and wound healing process after oral surgery procedure-A review paper","volume":"30","author":"Dimova","year":"2020","journal-title":"J. Hyg. Eng. Des."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.identj.2022.02.005","article-title":"Characteristics of suture materials used in oral surgery: Systematic review","volume":"72","author":"Faris","year":"2022","journal-title":"Int. Dent. J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.actbio.2023.07.041","article-title":"Advances, challenges, and prospects for surgical suture materials","volume":"168","author":"Li","year":"2023","journal-title":"Acta Biomater."},{"key":"ref_9","first-page":"9","article-title":"Efficacy of cyanoacrylate in intraoral surgical wound closure","volume":"34","author":"Mansoor","year":"2022","journal-title":"Age (Years)"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"587","DOI":"10.5005\/jp-journals-10015-2091","article-title":"Tissue adhesive or suture for wound closure following surgical removal of an impacted mandibular third molar: A randomized comparative study","volume":"13","author":"Thoniyottupurayil","year":"2022","journal-title":"World J. Dent."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e70047","DOI":"10.1111\/iwj.70047","article-title":"Tissue adhesives for closure of intraoral surgical incisions: A systematic review and meta-analysis","volume":"21","author":"Medeiros","year":"2024","journal-title":"Int. Wound J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3609","DOI":"10.1007\/s00784-020-03683-w","article-title":"Effect of cyanoacrylate tissue adhesive in postoperative palatal pain management: A systematic review","volume":"25","author":"Escobar","year":"2021","journal-title":"Clin. Oral Investig."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1016\/j.jds.2024.11.013","article-title":"Bioactive materials for post-operative healing and pain relief following palatal epithelialized graft harvesting: A meta-analysis of randomized clinical trials","volume":"20","author":"Li","year":"2025","journal-title":"J. Dent. Sci."},{"key":"ref_14","first-page":"31","article-title":"Design of Collagen-Based Sponge Device for Use in Oral Surgery","volume":"10","author":"Kuroyanagi","year":"2021","journal-title":"Open J. Regen. Med."},{"key":"ref_15","first-page":"9804","article-title":"Comparative analysis of the use of domestic bioresorbable collagen membranes at the closure of postoperative defects of the oral mucosa in an experiment in vivo","volume":"11","author":"Blagushina","year":"2021","journal-title":"Biointerface Res. Appl. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1016\/j.ijom.2023.11.009","article-title":"Posterior open wound healing in immediate implant placement using reactive soft tissue versus absorbable collagen sponge: A retrospective cohort study","volume":"53","author":"Deng","year":"2024","journal-title":"Int. J. Oral Maxillofac. Surg."},{"key":"ref_17","first-page":"e551","article-title":"Effect of fibrin-rich plasma and collagen sponge on healing of the palatal mucosa","volume":"15","author":"Guerrero","year":"2023","journal-title":"J. Clin. Exp. Dent."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1007\/s00784-020-03254-z","article-title":"Alvogyl and absorbable gelatin sponge as palatal wound dressings following epithelialized free gingival graft harvest: A randomized clinical trial","volume":"24","author":"Ehab","year":"2020","journal-title":"Clin. Oral Investig."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1002\/JPER.20-0645","article-title":"Healing of donor sites of connective tissue grafts harvested by the single incision technique: A randomized clinical trial evaluating the use of collagen hemostatic sponge with or without sutures","volume":"92","author":"Schinini","year":"2021","journal-title":"J. Periodontol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1002\/JPER.18-0475","article-title":"A randomized clinical trial of cyanoacrylate tissue adhesives in donor site of connective tissue grafts","volume":"90","author":"Stavropoulou","year":"2019","journal-title":"J. Periodontol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"56","DOI":"10.51847\/kFJklR9Vtt","article-title":"Wound Healing of Cyanoacrylate vs Collagen Among Patients Undergoing Oral Surgery; A Systematic Review","volume":"11","author":"Ansari","year":"2023","journal-title":"Ann. Dent. Spec."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"274","DOI":"10.5051\/jpis.2018.48.5.274","article-title":"Early Wound Healing Score: A system to evaluate the early healing of periodontal soft tissue wounds","volume":"48","author":"Marini","year":"2018","journal-title":"J. Periodontal Implant. Sci."},{"key":"ref_23","first-page":"209","article-title":"Task Force on Design and Analysis in Oral Health Research. The OHStat guidelines for reporting observational studies and clinical trials in oral health research: Explanation and elaboration","volume":"10","author":"Best","year":"2025","journal-title":"JDR Clin. Transl. Res."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Castro-Gaspar, C., Olmedo-Gaya, M.V., Romero-Olid, M.N., Lisbona-Gonzalez, M.J., Vallecillo-Rivas, M., and Reyes-Botella, C. (2021). Comparison between tissue adhesive cyanoacrylate and suture for palatal fibromucosa healing: A randomized controlled study. Materials, 14.","DOI":"10.3390\/ma14227009"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.bjps.2021.08.015","article-title":"Use of cyanoacrylate tissue adhesives for wound closure in the head and neck region: A systematic review","volume":"75","author":"Raj","year":"2022","journal-title":"J. Plast. Reconstr. Aesthet. Surg."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1111\/j.1834-7819.1997.tb00130.x","article-title":"Clinical and electron microscope comparison of silk sutures and n-butyl-2-cyanoacrylate in human mucosa","volume":"42","author":"Giray","year":"1997","journal-title":"Aust. Dent. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.joms.2012.08.032","article-title":"Healing of intraoral wounds closed using silk sutures and isoamyl 2-cyanoacrylate glue: A comparative clinical and histologic study","volume":"71","author":"Vastani","year":"2013","journal-title":"J. Oral Maxillofac. Surg."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"588","DOI":"10.21608\/adjc.2023.188851.1250","article-title":"Histological and immunohistochemical study on the effect of cyanoacrylate topical adhesive on healing of skin wound versus vicryl suture among rabbits","volume":"5","author":"Selim","year":"2023","journal-title":"Adv. Dent. J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"42","DOI":"10.4103\/ams.ams_192_20","article-title":"Efficacy of collagen membrane graft in intraoral surgery-an evaluative study","volume":"11","author":"Movaniya","year":"2021","journal-title":"Ann. Maxillofac. Surg."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1051\/mbcb\/2023041","article-title":"Comparative evaluation of healing after surgical excision of oral mucosal lesions using PRF and collagen membrane","volume":"29","author":"Poddar","year":"2023","journal-title":"J. Oral Med. Oral Surg."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"8217602","DOI":"10.1155\/2019\/8217602","article-title":"Oral applications of cyanoacrylate adhesives: A literature review","volume":"2019","author":"Borie","year":"2019","journal-title":"BioMed Res. Int."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"71","DOI":"10.61186\/jrdms.9.2.71","article-title":"Effect of Cyanoacrylate Adhesive on Palatal Wound Healing Following Free Gingival Grafting: A Clinical Trial","volume":"9","author":"Karimi","year":"2024","journal-title":"J. Res. Dent. Maxillofac. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1111\/j.1600-051X.2011.01823.x","article-title":"Impact of a collagen matrix on early healing, aesthetics and patient morbidity in oral mucosal wounds\u2013A randomized study in humans","volume":"39","author":"Thoma","year":"2012","journal-title":"J. Clin. Periodontol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4499","DOI":"10.1007\/s00784-022-04414-z","article-title":"Biomechanical evaluation of cyanoacrylate-based tissue adhesive for intraoral wound closure","volume":"26","author":"Becker","year":"2022","journal-title":"Clin. Oral Investig."}],"container-title":["Medicina"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1648-9144\/62\/5\/997\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T10:33:43Z","timestamp":1779878023000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1648-9144\/62\/5\/997"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,20]]},"references-count":34,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["medicina62050997"],"URL":"https:\/\/doi.org\/10.3390\/medicina62050997","relation":{},"ISSN":["1648-9144"],"issn-type":[{"value":"1648-9144","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,20]]}}}