{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T11:00:09Z","timestamp":1780657209896,"version":"3.54.1"},"reference-count":36,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objectives: Migraine is a chronic, throbbing type of headache that affects large populations worldwide. This condition is associated with neuroinflammation. Materials and Methods: In this study, polymorphism analyses were performed by KASP PCR. Serum interleukin-6 (IL-6) and 8-hydroxy-2\u2032-deoxyguanosine (8-OHdG) levels were measured using kits based on the enzyme-linked immunosorbent assay (ELISA) principle. Results: In the APE1 Asp148Glu (rs1130409) gene polymorphism analysis, the frequency of the mutant G (Glu) allele was 93.1% and 48.0% in the control and migraine populations, respectively, while the frequency of the wild-type T (Asp) allele was 6.9% and 52.0% (p &lt; 0.001). The frequency of the T\/T (Asp\/Asp) genotype was high in the migraine group (p &lt; 0.001), while the frequency of the G\/G (Glu\/Glu) genotype was higher in the control group at 86.2%, compared to the migraine group (p &lt; 0.001). The total frequency of the T\/G+ G\/G (Asp\/Glu+Glu\/Glu) composite genotype was determined to be 65.9% in the control group and 34.1% in the migraine group (p &lt; 0.001). There was no statistical difference in allele and genotype frequency between the control and migraine groups for the XRCC1 Arg399Gln (rs25487) gene polymorphism. Serum 8-OHdG and IL-6 levels were comparable between the groups, with no statistically significant differences observed. Conclusions: Future studies with larger and more homogeneous populations are needed to further elucidate the potential interactions between inflammatory processes and DNA damage in migraine. Consideration of attack duration and environmental exposures may improve interpretation of biomarker variability. Expanding the analysis to additional DNA repair gene polymorphisms may also contribute to a better understanding of the molecular background of migraine and the evaluation of potential biomarkers.<\/jats:p>","DOI":"10.3390\/medicina62061099","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T08:43:04Z","timestamp":1780648984000},"page":"1099","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Determination of 8-OHdG and IL-6 Levels, and of APE1 and XRCC1 DNA Repair Gene Variants, in Patients with Migraine"],"prefix":"10.3390","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6003-5975","authenticated-orcid":false,"given":"Tuba","family":"Gul","sequence":"first","affiliation":[{"name":"Department of Neurology, School of Medicine, Ordu University, Ordu 52200, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9779-8239","authenticated-orcid":false,"given":"Sukran","family":"Kaygisiz","sequence":"additional","affiliation":[{"name":"Department of Neurology, School of Medicine, Ordu University, Ordu 52200, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5201-6352","authenticated-orcid":false,"given":"Gonca","family":"Gulbay","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, School of Medicine, Ordu University, Ordu 52200, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7360-8090","authenticated-orcid":false,"given":"Yasemin","family":"Kaya","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, School of Medicine, Ordu University, Ordu 52200, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e70033","DOI":"10.1096\/fba.2024-00188","article-title":"The Role of Inflammation in Migraine Headaches: A Review","volume":"7","author":"Morgan","year":"2025","journal-title":"FASEB Bioadv."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Dominguez, L.J., Veronese, N., Sabico, S., Al-Daghri, N.M., and Barbagallo, M. (2025). Magnesium and Migraine. Nutrients, 17.","DOI":"10.3390\/nu17040725"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1186\/s10194-024-01889-x","article-title":"Hallmarks of primary headache: Part 1\u2014Migraine","volume":"25","author":"Raggi","year":"2024","journal-title":"J. Headache Pain"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1186\/s10194-026-02372-5","article-title":"The mechanism of different microglial receptors mediating central inflammation in chronic migraine: A meta-analysis","volume":"27","author":"Lin","year":"2026","journal-title":"J. Headache Pain"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"G\u00f6bel, C.H., Kuhn, K.H., Heinze, A., Cirkel, A., and G\u00f6bel, H. (2025). The Impact of Biseasonal Time Changes on Migraine. Neurol. Int., 17.","DOI":"10.3390\/neurolint17030040"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"111557","DOI":"10.1016\/j.biopha.2021.111557","article-title":"Genetics, pathophysiology, diagnosis, treatment, management, and prevention of migraine","volume":"139","author":"Khan","year":"2021","journal-title":"Biomed. Pharmacother."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Dermitzakis, E.V., Kouroudi, A., Argyriou, A.A., Spingos, K.C., Bilias, K., and Vikelis, M. (2022). Results of a Web-Based Survey on 2105 Greek Migraine Patients\u2014Second Part: Efficacy of Acute and Prophylactic Migraine Treatments and Corresponding Patients\u2019 Reported Satisfaction. Medicina, 59.","DOI":"10.3390\/medicina59010031"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1186\/s10194-026-02357-4","article-title":"Mechanistic investigation of HMGB1 in an in vitro model of the trigeminovascular system under migraine-like conditions","volume":"27","author":"Song","year":"2026","journal-title":"J. Headache Pain"},{"key":"ref_9","first-page":"99","article-title":"Relationship of Vitamin B12 and Vitamin D with IL-4, IL-10, TNF Beta in Obese Patients","volume":"15","author":"Gulbay","year":"2023","journal-title":"Konuralp Med. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"910","DOI":"10.5455\/medscience.2023.07.115","article-title":"Effects of lipids on inflammation and DNA damage in diabetes mellitus patients","volume":"12","author":"Gulbay","year":"2023","journal-title":"Med. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s12964-023-01398-5","article-title":"Interplay of oxidative stress, cellular communication and signaling pathways in cancer","volume":"22","author":"Iqbal","year":"2024","journal-title":"Cell Commun. Signal."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.17826\/cumj.1102599","article-title":"DNA damage and inflammation in COVID-19 cases","volume":"47","author":"Savrun","year":"2022","journal-title":"Cukurova Med. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/s10194-024-01750-1","article-title":"Unveiling the therapeutic potential of Dl-3-n-butylphthalide in NTG-induced migraine mouse: Activating the Nrf2 pathway to alleviate oxidative stress and neuroinflammation","volume":"25","author":"Liu","year":"2024","journal-title":"J. Headache Pain"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1186\/s12967-025-07055-4","article-title":"Cardiovascular comorbidities are risk factors for increased oxidative stress and DNA damage in migraine patients: A prospective cohort study","volume":"23","author":"Gokce","year":"2025","journal-title":"J. Transl. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1186\/s10194-025-02136-7","article-title":"Distinct inflammatory profiles across migraine states: A systematic review and meta-analysis","volume":"26","author":"Pereira","year":"2025","journal-title":"J. Headache Pain"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1186\/s10194-024-01833-z","article-title":"The role of kynurenines in migraine-related neuroimmune pathways","volume":"25","year":"2024","journal-title":"J. Headache Pain"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1177\/03331024221118924","article-title":"The role of cytokines in migraine: A systematic review","volume":"42","author":"Thuraiaiyah","year":"2022","journal-title":"Cephalalgia"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s10194-023-01572-7","article-title":"Cytokines in primary headache disorders: A systematic review and meta-analysis","volume":"24","author":"Musubire","year":"2023","journal-title":"J. Headache Pain"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1146\/annurev-biochem-052621-091205","article-title":"Transcription-Coupled Nucleotide Excision Repair and the Transcriptional Response to UV-Induced DNA Damage","volume":"92","author":"Olthof","year":"2023","journal-title":"Annu. Rev. Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1146\/annurev.biochem.73.011303.073723","article-title":"Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints","volume":"73","author":"Sancar","year":"2004","journal-title":"Annu. Rev. Biochem."},{"key":"ref_21","first-page":"1219","article-title":"The Investigation of Polymorphisms in DNA Repair Genes (XRCC1. APE1 and XPD) in Women with Polycystic Ovary Syndrome","volume":"18","author":"Gulbay","year":"2017","journal-title":"Asian Pac. J. Cancer Prev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"103679","DOI":"10.1016\/j.dnarep.2024.103679","article-title":"DNA repair deficiencies and neurodegeneration","volume":"138","author":"Ropert","year":"2024","journal-title":"DNA Repair"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S0065-3233(04)69002-4","article-title":"Nucleotide excision repair in E. coli and Man","volume":"69","author":"Sancar","year":"2004","journal-title":"Adv. Protein Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1038\/ncpneuro0448","article-title":"Mechanisms of disease: DNA repair defects and neurological disease","volume":"3","year":"2007","journal-title":"Nat. Clin. Pract. Neurol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1177\/10738584221090836","article-title":"DNA Damage and Repair in Migraine: Oxidative Stress and Beyond","volume":"29","author":"Fila","year":"2023","journal-title":"Neuroscientist"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Altwayan, R., Tombuloglu, H., Alhusil, A., Awadh, T., Altwayan, M., Albaqawi, H., Aldossary, N., and Unver, T. (2023). KASP-PCR method to screen thrombophilia genetic risk factors. medRxiv.","DOI":"10.1101\/2023.10.25.23297518"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"367","DOI":"10.2147\/NDT.S156710","article-title":"Oxidative\/antioxidative status. lymphocyte DNA damage. and urotensin-2 receptor level in patients with migraine attacks","volume":"14","author":"Yigit","year":"2018","journal-title":"Neuropsychiatr. Dis. Treat."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"102208","DOI":"10.1016\/j.aprim.2021.102208","article-title":"Migraine Review for General Practice","volume":"54","year":"2022","journal-title":"Aten. Primaria"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.neulet.2017.02.062","article-title":"Mitochondrial transcription factor A (TFAM) rs1937 and AP endonuclease 1 (APE1) rs1130409 alleles are associated with reduced cognitive performance","volume":"645","author":"Lillenes","year":"2017","journal-title":"Neurosci. Lett."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.neulet.2008.07.047","article-title":"Single nucleotide polymorphisms in base-excision repair genes hOGG1, APE1 and XRCC1 do not alter risk of Alzheimer\u2019s disease","volume":"442","author":"Karpuzoglu","year":"2008","journal-title":"Neurosci. Lett."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"135203","DOI":"10.1016\/j.neulet.2020.135203","article-title":"The impacts of prominent gene polymorphisms in DNA repair enzymes on Parkinson\u2019s disease","volume":"735","author":"Aslan","year":"2020","journal-title":"Neurosci. Lett."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1089\/ars.2005.7.367","article-title":"The intracellular localization of APE1\/Ref-1: More than a passive phenomenon?","volume":"7","author":"Tell","year":"2005","journal-title":"Antioxid. Redox Signal."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1093\/carcin\/22.6.917","article-title":"Amino acid substitution variants of APE1 and XRCC1 genes associated with ionizing radiation sensitivity","volume":"22","author":"Hu","year":"2001","journal-title":"Carcinogenesis"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1177\/0960327112444476","article-title":"Impact of DNA polymorphisms in key DNA base excision repair proteins on cancer risk","volume":"31","author":"Karahalil","year":"2012","journal-title":"Hum. Exp. Toxicol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.arcmed.2011.04.005","article-title":"Genetic polymorphism of DNA base-excision repair genes (APE1, OGG1 and XRCC1) and their correlation with risk of lung cancer in a Chinese population","volume":"42","author":"Li","year":"2011","journal-title":"Arch. Med. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"8344","DOI":"10.1007\/s12035-024-04114-7","article-title":"Oxidative Stress and Migraine","volume":"61","year":"2024","journal-title":"Mol. Neurobiol."}],"container-title":["Medicina"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1648-9144\/62\/6\/1099\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T09:59:48Z","timestamp":1780653588000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1648-9144\/62\/6\/1099"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,5]]},"references-count":36,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["medicina62061099"],"URL":"https:\/\/doi.org\/10.3390\/medicina62061099","relation":{},"ISSN":["1648-9144"],"issn-type":[{"value":"1648-9144","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,5]]}}}