{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:14:31Z","timestamp":1785413671086,"version":"3.56.0"},"reference-count":43,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T00:00:00Z","timestamp":1785024000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004686","name":"King Faisal University","doi-asserted-by":"publisher","award":["KFU262523"],"award-info":[{"award-number":["KFU262523"]}],"id":[{"id":"10.13039\/501100004686","id-type":"DOI","asserted-by":"publisher"}]},{"award":["KFU262523"],"award-info":[{"award-number":["KFU262523"]}],"id":[{"id":"https:\/\/ror.org\/00dn43547","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objectives: Blood transfusion is a key component of sickle cell disease (SCD) management, and ABO and Rh compatibility is essential to minimize transfusion-related complications. This study investigated factors associated with blood transfusion history and examined the relationships of ABO and Rh blood groups with clinical and laboratory markers of disease severity. Materials and Methods: A cross-sectional study was conducted among 309 patients attending a hematology outpatient clinic in Saudi Arabia between October and December 2025. Clinical data were collected through patient interviews, and laboratory results were obtained from medical records. Results: Overall, 89.9% of patients had a history of blood transfusion. Older age, chronic disease, higher platelet count, MCV, MCH, and HbA2 levels were associated with blood transfusion history. In contrast, higher hemoglobin and HbF levels were associated with fewer transfusions. Patients with blood group O had lower odds of transfusion than those with non-O blood groups (OR = 0.340, 95% CI: 0.135\u20130.856; p = 0.022). Hydroxyurea use was associated with lower LDH levels, while G6PD deficiency and higher MCV were independently associated with higher LDH levels. ABO and Rh blood groups were not associated with acute chest syndrome or LDH levels. Conclusions: Blood transfusion history in SCD was associated with several clinical and hematological factors. These findings support further investigation of G6PD status and reinforce the known benefits of hydroxyurea. Further studies are needed to clarify the role of ABO and Rh blood groups in disease progression and transfusion practices.<\/jats:p>","DOI":"10.3390\/medicina62081449","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T01:26:23Z","timestamp":1785115583000},"page":"1449","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Blood Transfusion and ABO Blood Groups in Sickle Cell Disease"],"prefix":"10.3390","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3975-5550","authenticated-orcid":false,"given":"Mortadah","family":"Alsalman","sequence":"first","affiliation":[{"name":"Department of Medicine, College of Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muthana","family":"AlSahlawi","sequence":"additional","affiliation":[{"name":"Department of Medicine, College of Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naushad","family":"Abid","sequence":"additional","affiliation":[{"name":"Department of Medicine, College of Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4632-891X","authenticated-orcid":false,"given":"Khaled","family":"Elzorkany","sequence":"additional","affiliation":[{"name":"Department of Medicine, College of Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman","family":"Abdallah","sequence":"additional","affiliation":[{"name":"Department of Medicine, College of Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alaa","family":"AlQuraini","sequence":"additional","affiliation":[{"name":"College of Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hussain Abduljaleel","family":"Alkhalifa","sequence":"additional","affiliation":[{"name":"Department of Medicine, King Fahad Specialist Hospital, Dammam 32253, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4596-5159","authenticated-orcid":false,"given":"Marwa","family":"Shafey","sequence":"additional","affiliation":[{"name":"Department of Family and Community Medicine, College of Medicine, Imam Abdulrahman bin Faisal University, Dammam 31441, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaenb","family":"Alsalman","sequence":"additional","affiliation":[{"name":"Department of Family and Community Medicine, College of Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/S0140-6736(12)61229-X","article-title":"Global epidemiology of sickle haemoglobin in neonates: A contemporary geostatistical model-based map and population estimates","volume":"381","author":"Piel","year":"2013","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Alhawiti, N.M., Shubair, M.M., Alharthy, A., Al-Khateeb, B.F., Aldahash, R., Aleissa, B., Angawi, K., AlJumah, M., Almutairi, A., and Almutairi, M.N. (2025). The prevalence and predictors of sickle cell anemia in the Saudi Arabia general population: Findings from a cross-sectional study. Healthcare, 13.","DOI":"10.3390\/healthcare13101117"},{"key":"ref_3","first-page":"e65263","article-title":"Prevalence, risk factors, and complications of sickle cell disease in Saudi Arabia: A systematic review","volume":"16","author":"Hamed","year":"2024","journal-title":"Cureus"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1186\/s12955-015-0380-8","article-title":"Health-related quality of life in patients with sickle cell disease in Saudi Arabia","volume":"13","author":"Ahmed","year":"2015","journal-title":"Health Qual. Life Outcomes"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Da Guarda, C.C., Yahou\u00e9d\u00e9hou, S., Santiago, R.P., Neres, J.S.D.S., Fernandes, C.F.D.L., Aleluia, M.M., Figueiredo, C.V.B., Fiuza, L.M., Carvalho, S.P., and De Oliveira, R.M. (2020). Sickle cell disease: A distinction of two most frequent genotypes (HbSS and HbSC). PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0228399"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"671","DOI":"10.2147\/JBM.S435861","article-title":"Predictors of intensive care admission among adult patients with sickle cell disease in Eastern Province of Saudi Arabia","volume":"14","author":"Alsalman","year":"2023","journal-title":"J. Blood Med."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Zayed, A.M., Almohaimeed, S., Alotaibi, T., Aldosari, H., Alotaibi, T., Ahmed, B., Abdullah, K., Awadallah, Y., Ancheta, S.J., and Arulantham, Z.J. (2025). Splenic sequestration crisis in children with sickle cell disease in the Eastern region of Saudi Arabia. BMC Pediatr., 25.","DOI":"10.1186\/s12887-025-06020-w"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.1056\/NEJM200006223422502","article-title":"Causes and outcomes of the acute chest syndrome in sickle cell disease","volume":"342","author":"Vichinsky","year":"2000","journal-title":"N. Engl. J. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"63","DOI":"10.25122\/jml-2023-0397","article-title":"Laboratory predictors of sickle cell disease severity: A cross-sectional study","volume":"17","author":"Alsalman","year":"2024","journal-title":"J. Med. Life"},{"key":"ref_10","first-page":"4","article-title":"Differences in the clinical and genotypic presentation of sickle cell disease around the world","volume":"15","author":"Saraf","year":"2014","journal-title":"Paediatr. Respir. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1046\/j.1365-3148.2001.00315.x","article-title":"The relationship between ABO histo-blood group, factor VIII and von Willebrand factor","volume":"11","author":"Laffan","year":"2001","journal-title":"Transfus. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1515\/cclm-2013-0168","article-title":"ABO blood group: Old dogma, new perspectives","volume":"51","author":"Franchini","year":"2013","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2018","DOI":"10.1016\/S0140-6736(10)61029-X","article-title":"Sickle-cell disease","volume":"376","author":"Rees","year":"2010","journal-title":"Lancet"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"28","DOI":"10.4103\/ajts.AJTS_107_20","article-title":"Von Willebrand factor levels in healthy blood donors and their association with blood group: A tertiary care hospital-based study","volume":"17","author":"Agrawal","year":"2023","journal-title":"Asian J. Transfus. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1056\/NEJMra1510865","article-title":"Sickle cell disease","volume":"376","author":"Piel","year":"2017","journal-title":"N. Engl. J. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1182\/blood.2019000944","article-title":"Glucose-6-phosphate dehydrogenase deficiency","volume":"136","author":"Luzzatto","year":"2020","journal-title":"Blood"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1038\/nm1202-799","article-title":"Cell-free hemoglobin limits nitric oxide bioavailability in sickle-cell disease","volume":"8","author":"Reiter","year":"2002","journal-title":"Nat. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1111\/bjh.12590","article-title":"Impact of glucose-6-phosphate dehydrogenase deficiency on sickle cell anaemia expression in infancy and early childhood: A prospective study","volume":"163","author":"Benkerrou","year":"2013","journal-title":"Br. J. Haematol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1080\/16078454.2022.2074715","article-title":"Does glucose-6-phosphate dehydrogenase deficiency worsen the clinical features of sickle cell disease? A multi-hospital-based cross-sectional study","volume":"27","author":"Opara","year":"2022","journal-title":"Hematology"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Curtis, S.A., Danda, N., Etzion, Z., Cohen, H.W., and Billett, H.H. (2015). Elevated steady-state WBC and platelet counts are associated with frequent emergency room use in adults with sickle cell anemia. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0133116"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Piel, F.B., Hay, S.I., Gupta, S., Weatherall, D.J., and Williams, T.N. (2013). Global burden of sickle cell anaemia in children under five, 2010\u20132050: Modelling based on demographics, excess mortality, and interventions. PLoS Med., 10.","DOI":"10.1371\/journal.pmed.1001484"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1182\/bloodadvances.2024013693","article-title":"Is allogeneic transplantation for sickle cell disease still relevant in the era of gene therapy?","volume":"9","author":"Jones","year":"2025","journal-title":"Blood Adv."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"103764","DOI":"10.1016\/j.transci.2023.103764","article-title":"Global burden of transfusion in sickle cell disease","volume":"62","author":"Inusa","year":"2023","journal-title":"Transfus. Apher. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.1182\/blood.2023023648","article-title":"How I treat challenging transfusion cases in sickle cell disease","volume":"145","author":"Chou","year":"2025","journal-title":"Blood"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"AlHumaidan, H., Al Zayed, A., Al Bahrani, A., Alsughayir, A.H., Algadeeb, K.B., Al Mozain, N., Kashari, O., Bin Ali, T.A.M., and Malhan, H. (2025). Position paper on the management of sickle cell disease in Saudi Arabia: Understanding disease landscape, identifying challenges, and exploring opportunities in transfusion therapies. J. Clin. Med., 14.","DOI":"10.3390\/jcm14103494"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1111\/bjh.14383","article-title":"Guidelines on red cell transfusion in sickle cell disease. Part II: Indications for transfusion","volume":"176","author":"Davis","year":"2017","journal-title":"Br. J. Haematol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1182\/bloodadvances.2019001143","article-title":"American Society of Hematology 2020 guidelines for sickle cell disease: Transfusion support","volume":"4","author":"Chou","year":"2020","journal-title":"Blood Adv."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"625","DOI":"10.2147\/CEOR.S276121","article-title":"Development of a severity classification system for sickle cell disease","volume":"12","author":"Shah","year":"2020","journal-title":"Clin. Outcomes Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"303","DOI":"10.2147\/CEOR.S511996","article-title":"Clinical and economic burden of managing patients with sickle cell disease receiving frequent red blood cell transfusions in the United States","volume":"17","author":"Udeze","year":"2025","journal-title":"Clin. Outcomes Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1002\/ajh.23232","article-title":"Genetic modifiers of sickle cell disease","volume":"87","author":"Steinberg","year":"2012","journal-title":"Am. J. Hematol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"635670","DOI":"10.1155\/2015\/635670","article-title":"Clinical Applications of Hemolytic Markers in the Differential Diagnosis and Management of Hemolytic Anemia","volume":"2015","author":"Barcellini","year":"2015","journal-title":"Dis. Markers"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"321","DOI":"10.2147\/JBM.S493425","article-title":"Utilization of Fetal Hemoglobin Parameters in Predicting Clinical Severity of Sickle Cell Disease: A Retrospective Study from a Tanzanian Cohort","volume":"16","author":"Haji","year":"2025","journal-title":"J. Blood Med."},{"key":"ref_33","first-page":"3819457","article-title":"Sickle Cell Beta-Plus Thalassemia with Subcapsular Hematoma of the Spleen","volume":"2017","author":"Dahal","year":"2017","journal-title":"Case Rep. Hematol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1179\/1607845412Y.0000000036","article-title":"Splenic Enlargement in Adults with Homozygous Sickle Cell Disease: The Jamaican Experience","volume":"18","author":"Asnani","year":"2013","journal-title":"Hematology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1002\/jgf2.31","article-title":"Diagnosis and Treatment of Macrocytic Anemias in Adults","volume":"18","author":"Nagao","year":"2017","journal-title":"J. Gen. Fam. Med."},{"key":"ref_36","first-page":"3179173","article-title":"Comorbidity of Glucose-6-Phosphate Dehydrogenase Deficiency and Sickle Cell Disease Exerts Significant Effect on RBC Indices","volume":"2019","author":"Adjei","year":"2019","journal-title":"Anemia"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1002\/ajh.25051","article-title":"Glucose-6-Phosphate Dehydrogenase Deficient Red Blood Cell Units Are Associated with Decreased Post-Transfusion Red Blood Cell Survival in Children with Sickle Cell Disease","volume":"93","author":"Sagiv","year":"2018","journal-title":"Am. J. Hematol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2961","DOI":"10.1182\/blood-2025-2961","article-title":"Systematic Intensification of Red Cell Exchange with Hydroxyurea Decreased the Transfusion Burden in Patients with Sickle Cell Disease: A Single-Center Retrospective Cohort Study","volume":"146","author":"Nica","year":"2025","journal-title":"Blood"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1111\/trf.18073","article-title":"Adding Hydroxyurea to Chronic Transfusion Therapy for Sickle Cell Anemia Reduces Transfusion Burden","volume":"65","author":"Nickel","year":"2025","journal-title":"Transfusion"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.15537\/1658-3175.1545","article-title":"Frequency of ABO Blood Groups in the Eastern Region of Saudi Arabia","volume":"22","author":"Bashwari","year":"2001","journal-title":"Saudi Med. J."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"26","DOI":"10.3390\/hemato5010004","article-title":"Patterns of Blood Transfusion in Sickle Cell Disease Hospitalizations","volume":"5","author":"Sharma","year":"2024","journal-title":"Hemato"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"6274","DOI":"10.1182\/bloodadvances.2021006283","article-title":"ABO Blood Group Type and Risk of Venous Thromboembolism in Patients with Cancer","volume":"6","author":"Englisch","year":"2022","journal-title":"Blood Adv."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"68","DOI":"10.34297\/AJBSR.2021.13.001834","article-title":"ABO\/Rh Blood Groups and Chronic Diseases in Angolan Patients","volume":"13","author":"Sacomboio","year":"2021","journal-title":"Am. J. Biomed. Sci. Res."}],"container-title":["Medicina"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1648-9144\/62\/8\/1449\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T01:50:34Z","timestamp":1785117034000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1648-9144\/62\/8\/1449"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,26]]},"references-count":43,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["medicina62081449"],"URL":"https:\/\/doi.org\/10.3390\/medicina62081449","relation":{},"ISSN":["1648-9144"],"issn-type":[{"value":"1648-9144","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,26]]}}}