{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T06:26:12Z","timestamp":1789021572133,"version":"build-2803163510"},"reference-count":35,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2018,6,21]],"date-time":"2018-06-21T00:00:00Z","timestamp":1529539200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/AMB\/50017-POCI-01-0145-FEDER-007638"],"award-info":[{"award-number":["UID\/AMB\/50017-POCI-01-0145-FEDER-007638"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/AMB\/50017-POCI-01-0145-FEDER-007638, UID\/BIA\/04325\/2013 \u2013 MEDTBIO, and UID\/BIM\/04773\/2013 CBMR"],"award-info":[{"award-number":["UID\/AMB\/50017-POCI-01-0145-FEDER-007638, UID\/BIA\/04325\/2013 \u2013 MEDTBIO, and UID\/BIM\/04773\/2013 CBMR"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicines"],"abstract":"<jats:p>Background: Essential oils (EOs) have shown antimicrobial, antioxidant, and antiproliferative activity, which may, alone or in combination with other substances, potentially be used for the development of new drugs. However, their chemical variability, depending on the species, varieties, or geographical origin (among other factors) determines different bioactivities that need to be evaluated. Methods: The antioxidant activity of Corymbia citriodora and eight Eucalyptus species EOs was determined using two different methods: the scavenging ability of 2,2\u2032-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS+\u2022) and peroxyl free radicals or oxygen radical absorbance capacity (ORAC). Antibacterial activity was evaluated using the microorganisms Streptococcus pneumoniae (strains D39 and TIGR4), and Haemophilus influenza (strain DSM 9999). The essential oils\u2019 minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was assessed using a microdilution method. The antiproliferative activity was determined using the THP-1 cell line (human acute monocytic leukaemia) with methylthiazolyldiphenyl-tetrazolium bromide assay (MTT). Results:Corymbia citriodora and Eucalyptus viminalis EOs showed the highest ABTS and peroxyl free radical scavenging capacity. Eucalyptus globulus EO showed a high potential to treat Streptococcus pneumoniae infections. Haemophilus influenzae was the respiratory pathogen that showed the highest resistance to all EOs, including tea tree EO. After 96 h of incubation, at 25 \u03bcg\/mL, Eucalyptus radiata and Eucalyptus viminalis EOs showed highest cytotoxic activity against the THP-1 cell line. Conclusions: Despite their specific bioactivities, no single EO showed simultaneously good antioxidant, antimicrobial, and antiproliferative activity.<\/jats:p>","DOI":"10.3390\/medicines5030061","type":"journal-article","created":{"date-parts":[[2018,6,22]],"date-time":"2018-06-22T02:46:21Z","timestamp":1529635581000},"page":"61","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Antibacterial, Antioxidant, and Antiproliferative Activities of Corymbia citriodora and the Essential Oils of Eight Eucalyptus Species"],"prefix":"10.3390","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2507-4228","authenticated-orcid":false,"given":"Maria Gra\u00e7a","family":"Miguel","sequence":"first","affiliation":[{"name":"Departamento de Qu\u00edmica e Farm\u00e1cia, Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, MeditBio, Campus de Gambelas, 8005-139 Faro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cust\u00f3dia","family":"Gago","sequence":"additional","affiliation":[{"name":"MeditBio, Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8913-6136","authenticated-orcid":false,"given":"Maria Dulce","family":"Antunes","sequence":"additional","affiliation":[{"name":"CEOT\/MeditBio Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Potugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soraia","family":"Lagoas","sequence":"additional","affiliation":[{"name":"Centre for Biomedical Research (CBMR), Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Potugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3878-6948","authenticated-orcid":false,"given":"Maria Leonor","family":"Faleiro","sequence":"additional","affiliation":[{"name":"Centre for Biomedical Research (CBMR), Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Potugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Meg\u00edas","sequence":"additional","affiliation":[{"name":"Instituto de la Grasa (C.S.I.C.), Universidad Pablo de Olavide, Edificio 46, Carretera de Utrera, km 1, 41013 Sevilla, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Cort\u00e9s-Giraldo","sequence":"additional","affiliation":[{"name":"Instituto de la Grasa (C.S.I.C.), Universidad Pablo de Olavide, Edificio 46, Carretera de Utrera, km 1, 41013 Sevilla, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0109-0584","authenticated-orcid":false,"given":"Javier","family":"Vioque","sequence":"additional","affiliation":[{"name":"Instituto de la Grasa (C.S.I.C.), Universidad Pablo de Olavide, Edificio 46, Carretera de Utrera, km 1, 41013 Sevilla, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3239-3190","authenticated-orcid":false,"given":"Ana Cristina","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Centro de Estudos do Ambiente e do Mar (CESAM Lisboa), Faculdade de Ci\u00eancias da Universidade de Lisboa, Centro de Biotecnologia Vegetal (CBV), DBV, C2, Campo Grande, 1749-016 Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,6,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Mulu, W., Yizengaw, E., Alemu, M., Mekonnen, D., Hailu, D., Ketemaw, K., Abera, B., and Kibret, M. (2018). Pharyngeal colonization and drug resistance profiles of Morraxella catarrhalis, Streptococcus pneumoniae, Staphylococcus aureus, and Haemophilus influenzae among HIV infected children attending ART clinic of felegehiwot referral hospital, Ethiopia. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0196722"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1055\/s-2005-925523","article-title":"Streptococcus pneumoniae: Epidemology, risk factors, and clinical features","volume":"26","author":"Hedlund","year":"2005","journal-title":"Semin. Respir. Crit. Care Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e459","DOI":"10.1002\/mbo3.459","article-title":"Antibacterial, antifungal, and antiviral effects of three essential oil blends","volume":"6","author":"Brochot","year":"2017","journal-title":"MicrobiologyOpen"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1002\/ffj.3252","article-title":"Essential oils in the treatment of respiratory tract diseases highlighting their role in bacterial infections and their anti-inflammatory action: A review","volume":"30","year":"2015","journal-title":"Flavour Fragr. J."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Chouhan, S., Sharma, K., and Guleria, S. (2017). Antimicrobial activity of some essential oils\u2014Present status and future perspectives. Medicines, 4.","DOI":"10.3390\/medicines4030058"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1111\/1574-6968.12440","article-title":"No induction of antimicrobial resistance in Staphylococcus aureus and Listeria monocytogenes during continuous exposure to eugenol and citral","volume":"354","author":"Faleiro","year":"2014","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2865","DOI":"10.1042\/CS20171246","article-title":"Oxidative stress and reactive oxygen species: A review of their role in ocular disease","volume":"131","author":"Ung","year":"2017","journal-title":"Clin. Sci."},{"key":"ref_8","first-page":"308re8","article-title":"Antioxidants can increase melanoma metastasis in mice","volume":"7","author":"Ibrahim","year":"2015","journal-title":"Sci. Transl. Med."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Blowman, K., Magalh\u00e3es, M., Lemos, M.F.L., Cabral, C., and Pires, I.M. (2018). Anticancer properties of essential oils and other natural products. Evid. Based Complement. Altern. Med., 3149362.","DOI":"10.1155\/2018\/3149362"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"185","DOI":"10.7751\/telopea19953017","article-title":"Systematic studies in the eucalypts. 7. A revision of the bloodwoods, genus Corymbia (Myrtaceae)","volume":"6","author":"Hill","year":"1995","journal-title":"Telopea"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0305-1978(97)00105-1","article-title":"The essential oils of the yellow bloodwood eucalypts (Corymbia, section Ochraria, Myrtaceae)","volume":"26","author":"Brophy","year":"1998","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Barbosa, L.C.A., Filomeno, C.A., and Teixeira, R.R. (2016). Chemical variability and biological activities of Eucalyptus spp. essential oils. Molecules, 21.","DOI":"10.3390\/molecules21121671"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/j.foodchem.2009.07.021","article-title":"Chemical composition and antimicrobial properties of essential oils of three Australian Eucalyptus species","volume":"119","author":"Gilles","year":"2010","journal-title":"Food Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.3390\/ph4121535","article-title":"Essential oils from different plant parts of Eucalyptus cinerea F. Muell. Ex Benth. (Myrtaceae) as a source of 1,8-cineole and their bioactivities","volume":"4","author":"Silva","year":"2011","journal-title":"Pharmaceuticals"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1002\/jsfa.8600","article-title":"Biological, medicinal and toxicological significance of Eucalyptus leaf essential oil: A review","volume":"98","author":"Dhakad","year":"2018","journal-title":"J. Sci. Food Agric."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.phytochem.2013.06.005","article-title":"Bioactivity against Bursaphelenchus xylophilus: Nematotoxics from essential oils, essential oils fractions and decoction waters","volume":"94","author":"Faria","year":"2013","journal-title":"Phytochemistry"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"12312","DOI":"10.3390\/molecules171012312","article-title":"Bioassays against Pinewood nematode: Assessment of a suitable dilution agent and screening for bioactive essential oils","volume":"17","author":"Barbosa","year":"2012","journal-title":"Molecules"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"61","DOI":"10.17660\/ActaHortic.2011.925.7","article-title":"Eucalyptus from Mata Experimental do Escaroupim (Portugal): Evaluation of the essential oil composition from sixteen species","volume":"925","author":"Faria","year":"2011","journal-title":"Acta Hortic."},{"key":"ref_19","unstructured":"Council of Europe (COE), and European Directorate for the Quality of Medicines (2007). European Pharmacopoeia, Council of Europe (COE). [6th ed.]."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0891-5849(98)00315-3","article-title":"Antioxidant activity applying an improved ABTS radical cation decolorization assay","volume":"26","author":"Re","year":"1999","journal-title":"Free Rad. Biol. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"6173","DOI":"10.1021\/jf904540m","article-title":"Effects of postharvest application of 1-MCP and postcutting dip treatment on the quality and nutritional properties of fresh-cut kiwifruit","volume":"58","author":"Antunes","year":"2010","journal-title":"J. Agric. Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"4619","DOI":"10.1021\/jf010586o","article-title":"Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe","volume":"49","author":"Ou","year":"2001","journal-title":"J. Agric. Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4437","DOI":"10.1021\/jf0201529","article-title":"High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format","volume":"50","author":"Huang","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"851721","DOI":"10.1155\/2015\/851721","article-title":"Antioxidant and antiproliferative activities of the essential oils from Thymbra capitata and Thymus species grown in Portugal","volume":"2015","author":"Miguel","year":"2015","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"8162","DOI":"10.1021\/jf0510079","article-title":"Antibacterial and antioxidant activities of essential oils isolated from Thymbra capitata L. (Cav.) and Origanum vulgare L.","volume":"53","author":"Faleiro","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"ref_27","unstructured":"Sena, I., Faria, J.M.S., Sanches, J., Trindade, H., Pedro, L.G., Barroso, J.G., and Figueiredo, A.C. (2012, January 5\u20138). Essential oil composition from twenty-six Eucalyptus taxa from Mata Experimental do Escaroupim (Portugal). Proceedings of the 43rd International Symposium on Essential Oils, Lisboa, Portugal."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Luis, A., Duarte, A.P., Pereira, L., and Domingues, F. (2017). Chemical profiling and evaluation of antioxidant and anti-microbial properties of selected commercial essential oils: A comparative study. Medicines, 4.","DOI":"10.3390\/medicines4020036"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1002\/ffj.3330","article-title":"Antioxidant synergism and antagonism between selected monoterpenes using the 2,2-diphenyl-1-picrylhydrazyl method","volume":"31","author":"Ciesla","year":"2016","journal-title":"Flavour Fragr. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"9088","DOI":"10.1021\/jf502857s","article-title":"Model studies on the antioxidant activity of common terpenoid constituents of essential oils by means of the 2,2-diphenyl-1-picrylhydrazyl method","volume":"62","author":"Wojtunik","year":"2014","journal-title":"J. Agric. Food Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"7672","DOI":"10.3390\/molecules16097672","article-title":"Antioxidant and antiacetylcholinesterase activities of some commercial essential oils and their major compounds","volume":"16","author":"Aazza","year":"2011","journal-title":"Molecules"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3943","DOI":"10.1021\/jf803358g","article-title":"Unusual antioxidant behavior of \u03b1- and \u03b3-terpinene in protecting methyl linoleate, DNA, and erythrocyte","volume":"57","author":"Li","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1007\/s12272-017-0933-y","article-title":"Pneumonia and Streptococcus pneumoniae vaccine","volume":"40","author":"Kim","year":"2017","journal-title":"Arch. Pharm. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1093\/jac\/dkp362","article-title":"Antimicrobial efficacy of eucalyptus oil and 1,8-cineole alone and in combination with chlorhexidine digluconate against microorganisms grown in planktonic and biofilm cultures","volume":"64","author":"Hendry","year":"2009","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_35","first-page":"587","article-title":"Anti-oxidant, anti-inflammatory and anti-proliferative activities of Moroccan commercial essential oils","volume":"9","author":"Aazza","year":"2014","journal-title":"Nat. Prod. Commun."}],"container-title":["Medicines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2305-6320\/5\/3\/61\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:09:32Z","timestamp":1760195372000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2305-6320\/5\/3\/61"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,21]]},"references-count":35,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2018,9]]}},"alternative-id":["medicines5030061"],"URL":"https:\/\/doi.org\/10.3390\/medicines5030061","relation":{},"ISSN":["2305-6320"],"issn-type":[{"value":"2305-6320","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,21]]}}}