{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T00:06:02Z","timestamp":1768521962065,"version":"3.49.0"},"reference-count":52,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,6,4]],"date-time":"2019-06-04T00:00:00Z","timestamp":1559606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicines"],"abstract":"<jats:p>Background: In this study, column chromatography was applied to separate active fractions from the ethyl acetate extract of Celastrus hindsii, a medicinal plant widely used in Southern China, Northern Vietnam, Myanmar, and Malaysia. Methods: Fourteen fractions from different dilutions of chloroform and methanol were separated by column chromatography and examined for biological activities. Results: It was found that a dilution of 50\u201370% methanol in chloroform yielded the highest total phenolics, flavonoids, and antioxidant activities (1,1-dipheny1-2-picrylhydrazyl (DPPH), 2,2-azinobis (3-ehtylbenzothiazoline-6-sulfonic acid), diammonium salt (ABTS) radical scavenging activity, and \u03b2-carotene bleaching method measured by lipid peroxidation inhibition). In addition, by gas chromatography-mass spectrometry (GC-MS) and electrospray ionization-mass spectrometry (ESI-MS) analyses, fifteen principal compounds from bioactive fractions belonging to fatty acids, amides, flavonoids, sterols, terpenes, and phenols were identified. Of these compounds, \u03b1-amyrin, \u03b2-amyrin, hydrazine carboxamide, hexadecanoic acid, fucosterol, (3\u03b2)-D:C-friedours-7-en-3-ol, rutin, and 2-hydroxy-1-ethyl ester accounted for maximal quantities, whilst concentrations of other constituents were &lt;5%. Conclusions: It is suggested that these identified compounds may greatly contribute to the antioxidant capacity of C. hindsii as well as its potential pharmaceutical properties.<\/jats:p>","DOI":"10.3390\/medicines6020064","type":"journal-article","created":{"date-parts":[[2019,6,5]],"date-time":"2019-06-05T09:37:58Z","timestamp":1559727478000},"page":"64","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Comprehensive Fractionation of Antioxidants and GC-MS and ESI-MS Fingerprints of Celastrus hindsii Leaves"],"prefix":"10.3390","volume":"6","author":[{"given":"Tran Duc","family":"Viet","sequence":"first","affiliation":[{"name":"Graduate School for International Development Cooperation (IDEC), Hiroshima University, Hiroshima 739-8529, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7103-5698","authenticated-orcid":false,"given":"Tran Dang","family":"Xuan","sequence":"additional","affiliation":[{"name":"Graduate School for International Development Cooperation (IDEC), Hiroshima University, Hiroshima 739-8529, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Truong Mai","family":"Van","sequence":"additional","affiliation":[{"name":"Graduate School for International Development Cooperation (IDEC), Hiroshima University, Hiroshima 739-8529, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6052-301X","authenticated-orcid":false,"given":"Yusuf","family":"Andriana","sequence":"additional","affiliation":[{"name":"Graduate School for International Development Cooperation (IDEC), Hiroshima University, Hiroshima 739-8529, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6610-4505","authenticated-orcid":false,"given":"Ramin","family":"Rayee","sequence":"additional","affiliation":[{"name":"Graduate School for International Development Cooperation (IDEC), Hiroshima University, Hiroshima 739-8529, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3563-2887","authenticated-orcid":false,"given":"Hoang-Dung","family":"Tran","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology, Nguyen Tat Thanh University, 298A-300A Nguyen Tat Thanh Street, Ward 13, District 4, Ho Chi Minh City 72820, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,6,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1039\/b000678p","article-title":"Celastraceae sesquiterpenoids: Biological activity and synthesis","volume":"31","author":"Spivey","year":"2002","journal-title":"Chem. 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