{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T03:24:11Z","timestamp":1789269851960,"version":"build-2803163510"},"reference-count":42,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T00:00:00Z","timestamp":1646352000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research","doi-asserted-by":"publisher","award":["BT\/PR24882\/NER\/95\/890\/2017)"],"award-info":[{"award-number":["BT\/PR24882\/NER\/95\/890\/2017)"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medical Sciences"],"abstract":"<jats:p>Anogeissus acuminata is used to treat wounds, diarrhoea, dysentery, and skin ailments. However, its hepatoprotective effect against ethanol-induced liver damage is yet to be reported. The phenolic-enriched ethyl acetate fraction of Anogeissus acuminata (AAE) was evaluated for hepatoprotective activity against ethanol-induced liver toxicity in rats. The intoxicated animals were treated with a phenolic-rich fraction of Anogeissus acuminata (AAE) (100 and 200 mg\/kg) and silymarin (100 mg\/kg). The antioxidant activity of AAE was analysed. Biochemical markers (ALT, AST, ALP, GGT, and TBL) for liver injury in ethanol-administered animals resulted in higher levels of key serum biochemical injury markers, as evidenced by increased levels of ALT (127.24 \u00b1 3.95), AST (189.54 \u00b1 7.56), ALP (263.88 \u00b1 12.96), GGT (91.65 \u00b1 3.96), and TBL (2.85 \u00b1 0.12) compared to Group I ALT (38.67 \u00b1 3.84), AST (64.45 \u00b1 5.97), GGT (38.67 \u00b1 3.84), and TBL (0.53 \u00b1 064) (p &lt; 0.05). AAE administration decreased serum biochemical liver injury markers as manifested in Group III animals\u2019 ALT (79.56 \u00b1 5.16), AST (151.76 \u00b1 6.16), ALP (184.67 \u00b1 10.12), GGT (68.24 \u00b1 4.05), TBL (1.66 \u00b1 0.082) (p &lt; 0.05), and Group IV ALT (55.54 \u00b1 4.35), AST (78.79 \u00b1 4.88), ALP (81.96 \u00b1 9.43), GGT (47.32 \u00b1 2.95), TBL (0.74 \u00b1 0.075) (p &lt; 0.05). Group IV exhibited the most significant reduction in serum biochemical markers as compared to Group III (p &lt; 0.05) and close to silymarin-treated Group V ALT (44.42 \u00b1 3.15), AST (74.45 \u00b1 5.75), ALP (67.32 \u00b1 9.14), GGT (42.43 \u00b1 2.54), TBL (0.634 \u00b1 0.077). Gene expression indices and histoarchitecture were evaluated to demonstrate the potential of AAE. The bioactive fraction of Anogeissus acuminata was rich in phenolics and flavonoid content. GC\u2013MS analysis identified gallic acid, palmitic acid, cis-10-heptadecenoic acid, 9-octadecenoic acid, epigallocatechin, 2,5-dihydroxyacetophenone, and catechin. Oral administration of AAE (100 and 200 mg\/kg) lowered the elevated levels of the biochemical markers and interleukin, and enhanced the level of enzymatic antioxidant. It also downregulated the expression level of proapoptotic genes and upregulated the expression level of the antiapoptotic gene along with improved liver histopathology.<\/jats:p>","DOI":"10.3390\/medsci10010017","type":"journal-article","created":{"date-parts":[[2022,3,6]],"date-time":"2022-03-06T20:40:02Z","timestamp":1646599202000},"page":"17","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Hepatoprotective and Antioxidant Potential of Phenolics-Enriched Fraction of Anogeissus acuminata Leaf against Alcohol-Induced Hepatotoxicity in Rats"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6076-0595","authenticated-orcid":false,"given":"Lal Chand","family":"Pal","sequence":"first","affiliation":[{"name":"Pharmacology Division, CSIR-National Botanical Research Institute, Lucknow 226001, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1584-691X","authenticated-orcid":false,"given":"Shivankar","family":"Agrawal","sequence":"additional","affiliation":[{"name":"Department of Phytochemistry, National Institute of Traditional Medicine, Indian Council of Medical Research (ICMR), Nehru Nagar, Belagavi 590010, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4787-2004","authenticated-orcid":false,"given":"Arti","family":"Gautam","sequence":"additional","affiliation":[{"name":"Pharmacology Division, CSIR-National Botanical Research Institute, Lucknow 226001, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayhind Kumar","family":"Chauhan","sequence":"additional","affiliation":[{"name":"Department of Zoology, MahilaMahavidyalaya, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0820-0560","authenticated-orcid":false,"given":"Chandana Venkateswara","family":"Rao","sequence":"additional","affiliation":[{"name":"Pharmacology Division, CSIR-National Botanical Research Institute, Lucknow 226001, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Nagappan, A., Kim, J.-H., Jung, D.Y., and Jung, M.H. 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