{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T12:15:25Z","timestamp":1783426525597,"version":"3.54.6"},"reference-count":31,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medical Sciences"],"abstract":"<jats:p>Background: Elastin-rich vessels such as the aorta pose a particular challenge for bipolar tissue sealing due to their high elasticity and low compressibility. It is unclear under which conditions a mechanically resilient seal can be achieved in such tissues. The aim of this experimental study was to investigate the sealability of elastin-rich aortic wall strips from pigs. The influence of compression pressure and impedance control on the resulting peel forces is analyzed. Methods: Porcine aortas were harvested immediately postmortem, segmented, and histologically characterized (hematoxylin\u2013eosin (HE) and elastin\u2013Van Gieson (EVG). Mechanical compressibility was determined uniaxially on full-wall disks (\u00d8 10 mm) and compared with carotid arteries \u00d8 &lt; 5 mm. Aortic strips (5 \u00d7 25 mm) were sealed bipolarly, first with a commercially available instrument. Further seals were performed employing a specially developed device that allowed defined compression pressures (2.5\u201310 kg) and variable final impedance settings (250 vs. 500 \u03a9). Sealing quality was quantified via peel force measurements. Statistical analysis was performed by Mann\u2013Whitney U tests. Results: The aortic wall showed a high elastin content (42.8 \u00b1 3.4%) and low compressibility (only 44.9% at 490 N), significantly lower than in carotid arteries (92.8% at 490 N). The standard instrument produced no stable seals at all. However, defined compression in the special setup led to significant improvements: mean peel force increased from 0.09 N (2.5 kg) to 0.71 N (10 kg; +68.8%). Increasing the interval between the initial and final impedance setting (500 \u03a9) further increased the peel force by 25.5% (0.32 \u00b1 0.05 N) at 2.5 kg compression. Combining compression of 5 kg and 500 \u03a9 final impedance interval achieved the highest stability (0.91 \u00b1 0.2 N; p &lt; 0.001). Histologically, vessel wall structures were not destroyed by the process; they remained largely intact. Coagulation zones were limited to the contact areas. Conclusions: Resilient bipolar sealing of elastin-rich aortic wall tissue is fundamentally possible but requires defined mechanical compression and adapted impedance control. As this study relied exclusively on ex vivo peel-force measurements, the findings should be interpreted as mechanistic rather than clinically validated. Functional assessments, such as burst pressure testing and evaluation in intact vessel segments, will be required in future work.<\/jats:p>","DOI":"10.3390\/medsci14030378","type":"journal-article","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T09:53:46Z","timestamp":1783418026000},"page":"378","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Experimental Investigation into Bipolar Sealability of Elastin-Rich Tissue"],"prefix":"10.3390","volume":"14","author":[{"given":"Andreas","family":"Kirschbaum","sequence":"first","affiliation":[{"name":"Department of Visceral, Thoracic, and Vascular Surgery, University Hospital Giessen and Marburg (UKGM), Marburg Site, D-35047 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gesche","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Department Human Medicine, Philipps University of Marburg, D-35047 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moritz","family":"Jesinghaus","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital Giessen and Marburg (UKGM), Marburg Site, D-35047 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3875-6367","authenticated-orcid":false,"given":"Nikolas","family":"Mirow","sequence":"additional","affiliation":[{"name":"Department Human Medicine, Philipps University of Marburg, D-35047 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8175","DOI":"10.1038\/s41598-025-93044-y","article-title":"Use of the vessel sealer in paraaortic lymphadenectomy in the robotic assisted approach in endometrial cancer","volume":"15","author":"Olszewski","year":"2025","journal-title":"Sci. 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