{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:41:17Z","timestamp":1778632877493,"version":"3.51.4"},"reference-count":21,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2019,4,23]],"date-time":"2019-04-23T00:00:00Z","timestamp":1555977600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medical Sciences"],"abstract":"<jats:p>This study reports (for the first time) the staining ability of vital (0.4% trypan blue and 1% neutral red) and fluorescent (Hoechst 33258) dyes to differentiate between live and dead Schistosoma haematobium (S. haematobium) eggs in human urine samples. Since S. haematobium egg is important in disease pathology, diagnosis, transmission, and drug development research, it is essential to be able to easily distinguish live eggs from dead ones. Staining is considered a way of enhancing the identification of live and dead eggs. Urine samples from school children were examined for the presence of S. haematobium eggs. Vital and fluorescent dyes were used to stain the samples that contained S. haematobium eggs, after which they were observed using light and fluorescent microscopes, respectively. The Hoechst 33258 provided a good staining outcome for differentiation between live and dead eggs, followed by 0.4% Trypan blue. Regarding the 1% neutral red stain, even though it provided some evidence of which egg was alive or dead, the distinction was not very clear; therefore, it could be useful when used in combination with other stains for egg viability determination. The benefits of this study will include assessing the effect of drugs on S. haematobium eggs in Schistosomiasis research.<\/jats:p>","DOI":"10.3390\/medsci7040064","type":"journal-article","created":{"date-parts":[[2019,4,24]],"date-time":"2019-04-24T03:14:28Z","timestamp":1556075668000},"page":"64","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Ability of Vital and Fluorescent Staining in the Differentiation of Schistosoma haematobium Live and Dead Eggs"],"prefix":"10.3390","volume":"7","author":[{"given":"Peter O.","family":"Forson","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra 00233, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3344-4869","authenticated-orcid":false,"given":"Patience B.","family":"Tetteh-Quarcoo","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra 00233, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Ahenkorah","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra 00233, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3643-4217","authenticated-orcid":false,"given":"Robert","family":"Aryee","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra 00233, Ghana"},{"name":"Department of Physiology, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra 00233, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esther N.","family":"Okine","sequence":"additional","affiliation":[{"name":"Central Laboratory Services, Korle-Bu Teaching Hospital, Korle-Bu, Accra 00233, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel","family":"Afutu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra 00233, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8387-5571","authenticated-orcid":false,"given":"Georgina I.","family":"Djameh","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Accra 00233, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey","family":"Agyapong","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Accra 00233, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abraham K.","family":"Anang","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Accra 00233, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick F.","family":"Ayeh-Kumi","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra 00233, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,4,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.1016\/S0140-6736(13)61949-2","article-title":"Human schistosomiasis","volume":"383","author":"Colley","year":"2014","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Sady, H., Al-Mekhlafi, H.M., Mahdy, M.A., Lim, Y.A., Mahmud, R., and Surin, J. (2013). Prevalence and associated factors of Schistosomiasis among children in Yemen: Implications for an effective control programme. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002377"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0001-706X(03)00029-9","article-title":"Quantification of clinical morbidity associated with schistosome infection in sub-Saharan Africa","volume":"86","author":"Brooker","year":"2003","journal-title":"Acta. Trop."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Mahmoud, A.A.F. (2001). The schistosomes and their intermediate hosts. Schistosomiasis, Imperial College Press.","DOI":"10.1142\/p126"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1016\/S0140-6736(06)69440-3","article-title":"Human schistosomiasis","volume":"368","author":"Gryseels","year":"2006","journal-title":"Lancet"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"725","DOI":"10.4269\/ajtmh.2002.66.725","article-title":"Randomized comparison of low-dose versus standard-dose praziquantel therapy in treatment of urinary tract morbidity due to Schistosoma haematobium infection","volume":"66","author":"King","year":"2002","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"22","DOI":"10.17554\/j.issn.2410-0579.2015.01.4","article-title":"Bladder cancer and urinary Schistosomiasis in Angola","volume":"1","author":"Botelho","year":"2015","journal-title":"J. Nephrol. Urol. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1111\/j.1365-3024.2009.01098.x","article-title":"Immunopathogenesis of human schistosomiasis","volume":"31","author":"Burke","year":"2009","journal-title":"Parasite Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e238","DOI":"10.3346\/jkms.2018.33.e238","article-title":"Comparison of ELISA and Urine Microscopy for Diagnosis of Schistosoma haematobium Infection","volume":"33","author":"Song","year":"2018","journal-title":"J. Korean Med. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1590\/S0074-02762006000900045","article-title":"Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni","volume":"101","author":"Sarvel","year":"2006","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.9734\/IJTDH\/2017\/29532","article-title":"Urinary Schistosomiasis and Its Related Anaemia among Children in a High-Risk Community in Ghana","volume":"22","author":"Gadri","year":"2017","journal-title":"Int. J. Trop. Dis. Health."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"151","DOI":"10.4236\/ojmm.2013.33023","article-title":"Urinary Schistosomiasis in Children\u2014Still a Concern in Part of the Ghanaian Capital City","volume":"3","author":"Attah","year":"2013","journal-title":"Open J. Med. Microbiol."},{"key":"ref_13","first-page":"7","article-title":"Urinary Schistosomiasis among Children in Murbai and Surbai Communities of Ardo-Kola Local Government Area, Taraba State, Nigeria","volume":"9831265","author":"Houmsou","year":"2016","journal-title":"J. Trop. Med."},{"key":"ref_14","unstructured":"Omnes, M.H., Dorange, G., Suquet, M., and Normant, Y. (, January 4\u20139). Application of staining techniques to improve the viability assessment of turbot (Psetta maxima) ova. Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway. Available online: https:\/\/archimer.ifremer.fr\/doc\/00002\/11323\/."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1017\/S0031182005009169","article-title":"Responses of the surface membrane and excretory system of Schistosoma mansoni to damage to treatment with praziquantel and other biomolecules","volume":"132","author":"Oliveira","year":"2006","journal-title":"Parasitology"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.ab.2015.09.010","article-title":"Application of a non-hazardous vital dye for cell counting with automated cell counters","volume":"492","author":"Kim","year":"2016","journal-title":"Anal. Biochem."},{"key":"ref_17","first-page":"9","article-title":"Validation of three viable-cell counting methods: Manual, semi-automated, and automated","volume":"7","author":"Perez","year":"2015","journal-title":"Biotechnology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2244","DOI":"10.1182\/blood.V87.6.2244.bloodjournal8762244","article-title":"The use of 7-aminoactinomycin D in identifying apoptosis: Simplicity of use and broad spectrum of application compared with other techniques","volume":"87","author":"Philpott","year":"1996","journal-title":"Blood"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Bucevi\u010dius, J., Lukinavi\u010dius, G., and Gerasimait\u0117, R. (2018). The Use of Hoechst Dyes for DNA Staining and Beyond. Chemosensors, 6.","DOI":"10.3390\/chemosensors6020018"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S1383-5769(00)00044-1","article-title":"Urinary schistosomiasis in southern Ghana: 1. Prevalence and morbidity assessment in three (defined) rural areas drained by the Densu river","volume":"49","author":"Aryeetey","year":"2000","journal-title":"Parasitol. Int."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.bjid.2014.11.004","article-title":"Impact of human schistosomiasis in sub-Saharan Africa","volume":"19","author":"Adenowo","year":"2015","journal-title":"Braz. J. Infect. Dis."}],"container-title":["Medical Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3271\/7\/4\/64\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:46:37Z","timestamp":1760186797000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3271\/7\/4\/64"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,23]]},"references-count":21,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2019,4]]}},"alternative-id":["medsci7040064"],"URL":"https:\/\/doi.org\/10.3390\/medsci7040064","relation":{},"ISSN":["2076-3271"],"issn-type":[{"value":"2076-3271","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,23]]}}}