{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T17:36:24Z","timestamp":1778261784273,"version":"3.51.4"},"reference-count":27,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,1,16]],"date-time":"2020-01-16T00:00:00Z","timestamp":1579132800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1078134; APP1103689"],"award-info":[{"award-number":["APP1078134; APP1103689"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medical Sciences"],"abstract":"<jats:p>Here we investigate whether the presence of germinal vesicle-stage oocytes (GV\u2212 oocytes) reflects poor oocyte developmental competence (or quality). This was a prospective, non-randomised, cohort pilot-study involving 60 patients undergoing in vitro fertilization\/ intracytoplasmic sperm injection for whom complete pregnancy outcome data were available. Patients in whom GV\u2212 oocytes were retrieved (GV+) at transvaginal oocyte retrieval (TVOR) were compared with those from whom no GVs were retrieved (GV\u2212). We found that GV+ (n = 29) and GV\u2212 (n = 31) patients were similarly aged (35.4 vs. 36.4 years; p = 0.446). GV+ patients had a mean of 2.41 \u00b1 2.03 GVs and comparable yields of MII oocytes to GV\u2212 patients (11 \u00b1 6.88 vs. 8.26 \u00b1 4.84; p = 0.077). Compared with GV\u2212 patients, GV+ patients had markedly lower implantation rates (11.8% vs. 30.2%; p = 0.022) as well as oocyte utilisation rates for clinical pregnancy (2.3% vs. 6.8%; p = 0.018) and live-birth (1.9% vs. 5.7%; p = 0.029). DNA damage levels measured using \u03b3H2AX immunostaining were not different in oocytes from women &lt;36 years versus those \u226536 years (p = 0.606). Thus, patients who have GV\u2212 stage oocytes at TVOR exhibit poor oocyte quality reflected in reduced per-oocyte pregnancy success rates and uniformly high levels of oocyte DNA damage.<\/jats:p>","DOI":"10.3390\/medsci8010004","type":"journal-article","created":{"date-parts":[[2020,1,17]],"date-time":"2020-01-17T04:14:41Z","timestamp":1579234481000},"page":"4","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The Presence of Immature GV\u2212 Stage Oocytes during IVF\/ICSI Is a Marker of Poor Oocyte Quality: A Pilot Study"],"prefix":"10.3390","volume":"8","author":[{"given":"Pia","family":"Astbury","sequence":"first","affiliation":[{"name":"Christopher Chen Oocyte Biology Research Laboratory, UQ Centre for Clinical Research, The University of Queensland, Herston 4029, Queensland, Australia"},{"name":"Queensland Fertility Group, Brisbane 4000, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2835-8464","authenticated-orcid":false,"given":"Goutham N.","family":"Subramanian","sequence":"additional","affiliation":[{"name":"Christopher Chen Oocyte Biology Research Laboratory, UQ Centre for Clinical Research, The University of Queensland, Herston 4029, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0130-8694","authenticated-orcid":false,"given":"Jessica","family":"Greaney","sequence":"additional","affiliation":[{"name":"Christopher Chen Oocyte Biology Research Laboratory, UQ Centre for Clinical Research, The University of Queensland, Herston 4029, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris","family":"Roling","sequence":"additional","affiliation":[{"name":"Queensland Fertility Group, Brisbane 4000, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqui","family":"Irving","sequence":"additional","affiliation":[{"name":"Queensland Fertility Group, Brisbane 4000, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayden A.","family":"Homer","sequence":"additional","affiliation":[{"name":"Christopher Chen Oocyte Biology Research Laboratory, UQ Centre for Clinical Research, The University of Queensland, Herston 4029, Queensland, Australia"},{"name":"Queensland Fertility Group, Brisbane 4000, Queensland, Australia"},{"name":"Reproductive Endocrinology &amp; Infertility Clinic, Royal Brisbane &amp; Women\u2019s Hospital, Brisbane 4029, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1126\/science.273.5271.59","article-title":"Oxidative stress, caloric restriction, and aging","volume":"273","author":"Sohal","year":"1996","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"172ra121","DOI":"10.1126\/scitranslmed.3004925","article-title":"Impairment of BRCA1-related DNA double-strand break repair leads to ovarian aging in mice and humans","volume":"5","author":"Titus","year":"2013","journal-title":"Sci. Transl. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4015874","DOI":"10.1155\/2017\/4015874","article-title":"Molecular Mechanisms Responsible for Increased Vulnerability of the Ageing Oocyte to Oxidative Damage","volume":"2017","author":"Mihalas","year":"2017","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1093\/humupd\/dmy002","article-title":"The importance of DNA repair for maintaining oocyte quality in response to anti-cancer treatments, environmental toxins and maternal ageing","volume":"24","author":"Winship","year":"2018","journal-title":"Hum. Reprod. Update"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1093\/humupd\/dmw028","article-title":"Ovarian ageing: The role of mitochondria in oocytes and follicles","volume":"22","author":"Boucret","year":"2016","journal-title":"Hum. Reprod. Update"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1093\/molehr\/gan064","article-title":"Revisiting oocyte-somatic cell interactions: In search of novel intrafollicular predictors and regulators of oocyte developmental competence","volume":"14","author":"Li","year":"2008","journal-title":"Mol. Hum. Reprod."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1017\/S0967199413000233","article-title":"Developmental potential of in vitro or in vivo matured oocytes","volume":"23","author":"Alcoba","year":"2015","journal-title":"Zygote"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1007\/s10815-012-9799-6","article-title":"The effect of immature oocytes quantity on the rates of oocytes maturity and morphology, fertilization, and embryo development in ICSI cycles","volume":"29","author":"Halvaei","year":"2012","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1016\/S0015-0282(00)01617-4","article-title":"In vitro maturation, fertilization, and development of human germinal vesicle oocytes collected from stimulated cycles","volume":"74","author":"Kim","year":"2000","journal-title":"Fertil. Steril."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1093\/humrep\/14.7.1859","article-title":"In-vitro matured metaphase-I oocytes have a lower fertilization rate but similar embryo quality as mature metaphase-II oocytes after intracytoplasmic sperm injection","volume":"14","author":"Joris","year":"1999","journal-title":"Hum. Reprod."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.fertnstert.2016.03.015","article-title":"Nondominant small follicles are a promising source of mature oocytes in modified natural cycle in vitro fertilization and embryo transfer","volume":"106","author":"Teramoto","year":"2016","journal-title":"Fertil. Steril."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1016\/j.fertnstert.2005.09.035","article-title":"A high number of oocytes obtained after ovarian hyperstimulation for in vitro fertilization or intracytoplasmic sperm injection is not associated with decreased pregnancy outcome","volume":"85","author":"Kok","year":"2006","journal-title":"Fertil. Steril."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1023\/A:1023670010031","article-title":"Oocyte and embryo quality in patients with excessive ovarian response during in vitro fertilization treatment","volume":"20","author":"Ng","year":"2003","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1007\/s10815-015-0547-6","article-title":"Double-strand DNA breaks and repair response in human immature oocytes and their relevance to meiotic resumption","volume":"32","author":"Coticchio","year":"2015","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1016\/j.fertnstert.2008.01.003","article-title":"From oocyte to baby: A clinical evaluation of the biological efficiency of in vitro fertilization","volume":"91","author":"Patrizio","year":"2009","journal-title":"Fertil. Steril."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2064","DOI":"10.1016\/j.fertnstert.2010.02.004","article-title":"Live babies born per oocyte retrieved in a subpopulation of oocyte donors with repetitive reproductive success","volume":"94","author":"Martin","year":"2010","journal-title":"Fertil. Steril."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/978-1-4939-8603-3_9","article-title":"Immunofluorescence Staining of K-Fibers in Mouse Oocytes Using Cold Fixation","volume":"1818","author":"Greaney","year":"2018","journal-title":"Methods Mol. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.1242\/dev.078352","article-title":"Spindle assembly checkpoint signalling is uncoupled from chromosomal position in mouse oocytes","volume":"139","author":"Gui","year":"2012","journal-title":"Development"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.devcel.2013.02.008","article-title":"Hec1-dependent cyclin B2 stabilization regulates the G2-M transition and early prometaphase in mouse oocytes","volume":"25","author":"Gui","year":"2013","journal-title":"Dev. Cell"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1126\/science.1175326","article-title":"A spindle assembly checkpoint protein functions in prophase I arrest and prometaphase progression","volume":"326","author":"Homer","year":"2009","journal-title":"Science"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/978-1-62703-191-2_12","article-title":"Immunofluorescence staining of spindles, chromosomes, and kinetochores in human oocytes","volume":"957","author":"Riris","year":"2013","journal-title":"Methods Mol. Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"4029","DOI":"10.1038\/s41467-018-06510-9","article-title":"Cdk1 inactivation induces post-anaphase-onset spindle migration and membrane protrusion required for extreme asymmetry in mouse oocytes","volume":"9","author":"Wei","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"5858","DOI":"10.1074\/jbc.273.10.5858","article-title":"DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139","volume":"273","author":"Rogakou","year":"1998","journal-title":"J. Biol. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1083\/jcb.146.5.905","article-title":"Megabase chromatin domains involved in DNA double-strand breaks in vivo","volume":"146","author":"Rogakou","year":"1999","journal-title":"J. Cell. Biol."},{"key":"ref_25","unstructured":"National Institute for Health and Care Excellence (2020, January 02). Fertility Problems: Assessment and Treatment (NICE Guideline 156) 2013. Available online: https:\/\/www.nice.org.uk\/guidance\/conditions-and-diseases\/fertility-pregnancy-and-childbirth\/fertility."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1093\/humupd\/dmx035","article-title":"Regulation of chromosome segregation in oocytes and the cellular basis for female meiotic errors","volume":"24","author":"Greaney","year":"2018","journal-title":"Hum. Reprod. Update"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1016\/j.fertnstert.2013.11.004","article-title":"The nature of aneuploidy with increasing age of the female partner: A review of 15,169 consecutive trophectoderm biopsies evaluated with comprehensive chromosomal screening","volume":"101","author":"Franasiak","year":"2014","journal-title":"Fertil. Steril."}],"container-title":["Medical Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3271\/8\/1\/4\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:43:09Z","timestamp":1760362989000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3271\/8\/1\/4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,16]]},"references-count":27,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,3]]}},"alternative-id":["medsci8010004"],"URL":"https:\/\/doi.org\/10.3390\/medsci8010004","relation":{},"ISSN":["2076-3271"],"issn-type":[{"value":"2076-3271","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,16]]}}}