{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:06:38Z","timestamp":1790719598569,"version":"4.1.0"},"reference-count":40,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,6,25]],"date-time":"2021-06-25T00:00:00Z","timestamp":1624579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Membranes"],"abstract":"<jats:p>Brain-derived neurotrophic factor (BDNF) has recently been recognized as a cardiovascular regulator particularly in the diseased condition, including coronary artery disease, heart failure, cardiomyopathy, and hypertension. Here, we investigate the role of BDNF on the T-type Ca2+ channel, Cav3.1 and Cav3.2, in rat neonatal cardiomyocytes exposed to normoxia (21% O2) and acute hypoxia (1% O2) in vitro for up to 3 h. The exposure of cardiomyocytes to hypoxia (1 h, 3 h) caused a significant upregulation of the mRNAs for hypoxia-inducible factor 1\u03b1 (Hif1\u03b1), Cav3.1, Cav3.2 and Bdnf, but not tropomyosin-related kinase receptor B (TrkB). The upregulation of Cav3.1 and Cav3.2 caused by hypoxia was completely halted by small interfering RNA (siRNA) targeting Hif1a (Hif1a-siRNA) or Bdnf (Bdnf-siRNA). Immunocytochemical staining data revealed a distinct upregulation of Cav3.1- and Cav3.2-proteins caused by hypoxia in cardiomyocytes, which was markedly suppressed by Bdnf-siRNA. These results unveiled a novel regulatory action of BDNF on the T-type Ca2+ channels expression through the HIF-1\u03b1-dependent pathway in cardiomyocytes.<\/jats:p>","DOI":"10.3390\/membranes11070470","type":"journal-article","created":{"date-parts":[[2021,6,25]],"date-time":"2021-06-25T11:07:40Z","timestamp":1624619260000},"page":"470","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Enhanced BDNF Actions Following Acute Hypoxia Facilitate HIF-1\u03b1-Dependent Upregulation of Cav3-T-Type Ca2+ Channels in Rat Cardiomyocytes"],"prefix":"10.3390","volume":"11","author":[{"given":"Masaki","family":"Morishima","sequence":"first","affiliation":[{"name":"Department of Food and Nutrition, Kindai University Faculty of Agriculture, Nara 631-8505, Japan"},{"name":"Department of Pathophysiology, Oita University School of Medicine, Oita 879-5593, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takafumi","family":"Fujita","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Oita University School of Medicine, Oita 879-5593, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Osagawa","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Oita University School of Medicine, Oita 879-5593, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Kubota","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Oita University School of Medicine, Oita 879-5593, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsushige","family":"Ono","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Oita University School of Medicine, Oita 879-5593, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,25]]},"reference":[{"key":"ref_1","unstructured":"Libby, P., and Braunwald, E. 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