{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T04:45:56Z","timestamp":1769834756532,"version":"3.49.0"},"reference-count":57,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,8,10]],"date-time":"2021-08-10T00:00:00Z","timestamp":1628553600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Membranes"],"abstract":"<jats:p>Periodontitis is a prevalent disease characterized by the loss of periodontal supporting tissues, bone, periodontal ligament, and cementum. The application of a bone tissue engineering strategy with Decellularized Human Amniotic Membrane (DAM) with adipose-derived stromal cells (ASCs) has shown to be convenient and valuable. This study aims to investigate the treatments of a rat periodontal furcation defect model with DAM, ASCs, and a mineralized extracellular matrix (ECM). Rat ASCs were expanded, cultivated on DAM, and with a bone differentiation medium for four weeks, deposited ECM on DAM. Periodontal healing for four weeks was evaluated by micro-computed tomography and histological analysis after treatments with DAM, ASCs, and ECM and compared to untreated defects on five consecutive horizontal levels, from gingival to apical. The results demonstrate that DAM preserves its structure during cultivation and healing periods, supporting cell attachment, permeation, bone deposition on DAM, and periodontal regeneration. DAM and DAM+ASCs enhance bone healing compared to the control on the gingival level. In conclusion, DAM with ASC or without cells and the ECM ensures bone tissue healing. The membrane supported neovascularization and promoted osteoconduction.<\/jats:p>","DOI":"10.3390\/membranes11080606","type":"journal-article","created":{"date-parts":[[2021,8,10]],"date-time":"2021-08-10T02:15:00Z","timestamp":1628561700000},"page":"606","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Adipose-Derived Stromal Cells and Mineralized Extracellular Matrix Delivery by a Human Decellularized Amniotic Membrane in Periodontal Tissue Engineering"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9528-5189","authenticated-orcid":false,"given":"Dilcele Silva Moreira","family":"Dziedzic","sequence":"first","affiliation":[{"name":"Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, The Pel\u00e9 Pequeno Pr\u00edncipe Research Institute, Child and Adolescent Health Research & Pequeno Pr\u00edncipe Faculties, Curitiba, Paran\u00e1 80250-060, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1338-0693","authenticated-orcid":false,"given":"Bassam Felipe","family":"Mogharbel","sequence":"additional","affiliation":[{"name":"Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, The Pel\u00e9 Pequeno Pr\u00edncipe Research Institute, Child and Adolescent Health Research & Pequeno Pr\u00edncipe Faculties, Curitiba, Paran\u00e1 80250-060, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5236-4928","authenticated-orcid":false,"given":"Ana Carolina","family":"Irioda","sequence":"additional","affiliation":[{"name":"Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, The Pel\u00e9 Pequeno Pr\u00edncipe Research Institute, Child and Adolescent Health Research & Pequeno Pr\u00edncipe Faculties, Curitiba, Paran\u00e1 80250-060, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2582-137X","authenticated-orcid":false,"given":"Priscila Elias Ferreira","family":"Stricker","sequence":"additional","affiliation":[{"name":"Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, The Pel\u00e9 Pequeno Pr\u00edncipe Research Institute, Child and Adolescent Health Research & Pequeno Pr\u00edncipe Faculties, Curitiba, Paran\u00e1 80250-060, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2617-7181","authenticated-orcid":false,"given":"Maiara Carolina","family":"Perussolo","sequence":"additional","affiliation":[{"name":"Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, The Pel\u00e9 Pequeno Pr\u00edncipe Research Institute, Child and Adolescent Health Research & Pequeno Pr\u00edncipe Faculties, Curitiba, Paran\u00e1 80250-060, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2525-7788","authenticated-orcid":false,"given":"C\u00e9lia Regina Cavichiolo","family":"Franco","sequence":"additional","affiliation":[{"name":"Cell Biology Department, Federal University of Paran\u00e1, Curitiba, Paran\u00e1 81530-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6098-6858","authenticated-orcid":false,"given":"Hsueh-Wen","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2964-6927","authenticated-orcid":false,"given":"Eltyeb","family":"Abdelwahid","sequence":"additional","affiliation":[{"name":"Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4860-2855","authenticated-orcid":false,"given":"Katherine Athayde Teixeira","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, The Pel\u00e9 Pequeno Pr\u00edncipe Research Institute, Child and Adolescent Health Research & Pequeno Pr\u00edncipe Faculties, Curitiba, Paran\u00e1 80250-060, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1243\/09544119JEIM820","article-title":"Periodontal regeneration: A challenge for the tissue engineer?","volume":"224","author":"Hughes","year":"2010","journal-title":"Proc. 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