{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T15:16:25Z","timestamp":1793114185631,"version":"4.5.0"},"reference-count":81,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,6,25]],"date-time":"2022-06-25T00:00:00Z","timestamp":1656115200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004329","name":"Slovenian Research Agency (ARRS)","doi-asserted-by":"publisher","award":["P1-0207"],"award-info":[{"award-number":["P1-0207"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research Agency (ARRS)","doi-asserted-by":"publisher","award":["J3-1747"],"award-info":[{"award-number":["J3-1747"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Membranes"],"abstract":"<jats:p>Periodontal disease is a chronic oral inflammatory disorder initiated by pathobiontic bacteria found in dental plaques\u2014complex biofilms on the tooth surface. The disease begins as an acute local inflammation of the gingival tissue (gingivitis) and can progress to periodontitis, which eventually leads to the formation of periodontal pockets and ultimately results in tooth loss. The main problem in periodontology is that the diagnosis is based on the assessment of the already obvious tissue damage. Therefore, it is necessary to improve the current diagnostics used to assess periodontal disease. Using lipidomic analyses, we show that both crucial periodontal pathogens, Porphyromonas gingivalis and Tannerella forsythia, synthesize ceramide phosphoethanolamine (CPE) species, membrane sphingolipids not typically found in vertebrates. Previously, it was shown that this particular lipid can be specifically detected by an aegerolysin protein, erylysin A (EryA). Here, we show that EryA can specifically bind to CPE species from the total lipid extract from P. gingivalis. Furthermore, using a fluorescently labelled EryA-mCherry, we were able to detect CPE species in clinical samples of dental plaque from periodontal patients. These results demonstrate the potential of specific periodontal pathogen-derived lipids as biomarkers for periodontal disease and other chronic inflammatory diseases.<\/jats:p>","DOI":"10.3390\/membranes12070655","type":"journal-article","created":{"date-parts":[[2022,6,26]],"date-time":"2022-06-26T21:01:41Z","timestamp":1656277301000},"page":"655","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Ceramide Phosphoethanolamine as a Possible Marker of Periodontal Disease"],"prefix":"10.3390","volume":"12","author":[{"given":"Maja","family":"Grundner","sequence":"first","affiliation":[{"name":"Department of Biology, Biotechnical Faculty, University of Ljubljana, Ve\u010dna pot 111, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haris","family":"Munjakovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Biology, Biotechnical Faculty, University of Ljubljana, Ve\u010dna pot 111, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tilen","family":"Tori","sequence":"additional","affiliation":[{"name":"Department of Biology, Biotechnical Faculty, University of Ljubljana, Ve\u010dna pot 111, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4023-5379","authenticated-orcid":false,"given":"Kristina","family":"Sep\u010di\u0107","sequence":"additional","affiliation":[{"name":"Department of Biology, Biotechnical Faculty, University of Ljubljana, Ve\u010dna pot 111, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3765-7378","authenticated-orcid":false,"given":"Rok","family":"Ga\u0161per\u0161i\u010d","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u010cedomir","family":"Oblak","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4126-7644","authenticated-orcid":false,"given":"Katja","family":"Seme","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1799-0819","authenticated-orcid":false,"given":"Graziano","family":"Guella","sequence":"additional","affiliation":[{"name":"Bioorganic Chemistry Laboratory, Department of Physics, University of Trento, Via Sommarive 14, 38123 Trento, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Trenti","sequence":"additional","affiliation":[{"name":"Bioorganic Chemistry Laboratory, Department of Physics, University of Trento, Via Sommarive 14, 38123 Trento, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matej","family":"Sko\u010daj","sequence":"additional","affiliation":[{"name":"Department of Biology, Biotechnical Faculty, University of Ljubljana, Ve\u010dna pot 111, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1111\/odi.12428","article-title":"Global burden of oral diseases: Emerging concepts, management and interplay with systemic health","volume":"22","author":"Jin","year":"2016","journal-title":"Oral Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1016\/S0140-6736(17)32154-2","article-title":"Global, regional, and national incidence, prevalence, and years lived with disability for 328 diseases and injuries for 195 countries, 1990\u20132016: A systematic analysis for the Global Burden of Disease Study 2016","volume":"390","author":"Vos","year":"2017","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1177\/0022034517693566","article-title":"Global, Regional, and National Prevalence, Incidence, and Disability-Adjusted Life Years for Oral Conditions for 195 Countries, 1990\u20132015: A Systematic Analysis for the Global Burden of Diseases, Injuries, and Risk Factors","volume":"96","author":"Kassebaum","year":"2017","journal-title":"J. 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