{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T19:59:07Z","timestamp":1778011147733,"version":"3.51.4"},"reference-count":63,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T00:00:00Z","timestamp":1679529600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 Research and Innovation Program","award":["812915"],"award-info":[{"award-number":["812915"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Membranes"],"abstract":"<jats:p>Chlamydia trachomatis is the bacterial pathogen that causes most cases of sexually transmitted diseases annually. To combat the global spread of asymptomatic infection, development of effective (mucosal) vaccines that offer both systemic and local immune responses is considered a high priority. In this study, we explored the expression of C. trachomatis full-length (FL) PmpD, as well as truncated PmpD passenger constructs fused to a \u201cdisplay\u201d autotransporter (AT) hemoglobin protease (HbpD) and studied their inclusion into outer membrane vesicles (OMVs) of Escherichia coli and Salmonella Typhimurium. OMVs are considered safe vaccine vectors well-suited for mucosal delivery. By using E. coli AT HbpD-fusions of chimeric constructs we improved surface display and successfully generated Salmonella OMVs decorated with a secreted and immunogenic PmpD passenger fragment (aa68-629) to 13% of the total protein content. Next, we investigated whether a similar chimeric surface display strategy could be applied to other AT antigens, i.e., secreted fragments of Prn (aa35-350) of Bordetella pertussis and VacA (aa65-377) of Helicobacter pylori. The data provided information on the complexity of heterologous expression of AT antigens at the OMV surface and suggested that optimal expression strategies should be developed on an antigen-to-antigen basis.<\/jats:p>","DOI":"10.3390\/membranes13040366","type":"journal-article","created":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T04:37:28Z","timestamp":1679546248000},"page":"366","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Heterologous Display of Chlamydia trachomatis PmpD Passenger at the Surface of Salmonella OMVs"],"prefix":"10.3390","volume":"13","author":[{"given":"Dung T.","family":"Huynh","sequence":"first","affiliation":[{"name":"Abera Bioscience AB, 750 26 Uppsala, Sweden"},{"name":"Department of Molecular Microbiology, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Vrije Universiteit, 1081 HV Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wouter S. P.","family":"Jong","sequence":"additional","affiliation":[{"name":"Abera Bioscience AB, 750 26 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manon A. H.","family":"Oudejans","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Vrije Universiteit, 1081 HV Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. Bart van den","family":"Berg van Saparoea","sequence":"additional","affiliation":[{"name":"Abera Bioscience AB, 750 26 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joen","family":"Luirink","sequence":"additional","affiliation":[{"name":"Abera Bioscience AB, 750 26 Uppsala, Sweden"},{"name":"Department of Molecular Microbiology, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Vrije Universiteit, 1081 HV Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2164-1611","authenticated-orcid":false,"given":"Peter van","family":"Ulsen","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Vrije Universiteit, 1081 HV Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3101","DOI":"10.3389\/fmicb.2018.03101","article-title":"Chlamydia Persistence: A Survival Strategy to Evade Antimicrobial Effects in-Vitro and in-Vivo","volume":"9","author":"Panzetta","year":"2018","journal-title":"Front. 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