{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T01:45:17Z","timestamp":1770687917988,"version":"3.49.0"},"reference-count":48,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,7,29]],"date-time":"2023-07-29T00:00:00Z","timestamp":1690588800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002597","name":"Kyung Hee University","doi-asserted-by":"publisher","award":["KHU-20201096"],"award-info":[{"award-number":["KHU-20201096"]}],"id":[{"id":"10.13039\/501100002597","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Membranes"],"abstract":"<jats:p>A cell filtration platform that affords accurate size separation and minimizes fouling was developed. The platform features an ultra-thin porous membrane (UTM) filter, a pumping head filtration with backflush (PHF), and cell size measurement (CSM) software. The UTM chip is an ultrathin free-standing membrane with a large window area of 0.68 mm2, a pore diameter of 5 to 9 \u03bcm, and a thickness of less than 0.9 \u03bcm. The PHF prevents filter fouling. The CSM software analyzes the size distributions of the supernatants and subnatants of isolated cells and presents the data visually. The D99 particle size of cells of the chronic myeloid leukemia (CML) line K562 decreased from 22.2 to 17.5 \u03bcm after passage through a 5-\u03bcm filter. K562 cells could be separated by careful selection of the pore size; the recovery rate attained 91.3%. The method was compared to conventional blocking models by evaluating the mean square errors (MSEs) between the measured and calculated filtering volumes. The filtering rate was fitted by a linear regression model with a significance that exceeded 0.99 based on the R2 value. The platform can be used to separate various soft biomaterials and afford excellent stability during filtration.<\/jats:p>","DOI":"10.3390\/membranes13080707","type":"journal-article","created":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T03:30:02Z","timestamp":1690774202000},"page":"707","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Precise Filtration of Chronic Myeloid Leukemia Cells by an Ultrathin Microporous Membrane with Backflushing to Minimize Fouling"],"prefix":"10.3390","volume":"13","author":[{"given":"Jaehyuk","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering, Kyung Hee University, Yongin 17104, Republic of Korea"},{"name":"R&D Center, Metapore Co., Ltd., Advanced Institutes of Convergence Technology 8F, Suwon 16229, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeongpyo","family":"Hong","sequence":"additional","affiliation":[{"name":"R&D Center, Metapore Co., Ltd., Advanced Institutes of Convergence Technology 8F, Suwon 16229, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jungwon","family":"Lee","sequence":"additional","affiliation":[{"name":"R&D Center, Metapore Co., Ltd., Advanced Institutes of Convergence Technology 8F, Suwon 16229, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changgyu","family":"Lee","sequence":"additional","affiliation":[{"name":"R&D Center, Metapore Co., Ltd., Advanced Institutes of Convergence Technology 8F, Suwon 16229, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tony","family":"Kim","sequence":"additional","affiliation":[{"name":"R&D Center, Metapore Co., Ltd., Advanced Institutes of Convergence Technology 8F, Suwon 16229, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young","family":"Jeong","sequence":"additional","affiliation":[{"name":"R&D Center, Metapore Co., Ltd., Advanced Institutes of Convergence Technology 8F, Suwon 16229, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwanghee","family":"Kim","sequence":"additional","affiliation":[{"name":"National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inhwa","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, Kyung Hee University, Yongin 17104, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Horade, M., Tsai, C.H.D., Ito, H., and Kaneko, M. (2017). Red Blood Cell Responses during a Long-Standing Load in a Microfluidic Constriction. Micromachines, 8.","DOI":"10.3390\/mi8040100"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"6999","DOI":"10.1038\/ncomms7999","article-title":"Chip-based analysis of exosomal mRNA mediating drug resistance in glioblastoma","volume":"6","author":"Shao","year":"2015","journal-title":"Nat. Commun."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"11227","DOI":"10.1021\/nn405016y","article-title":"Magnetic Nanosensor for Detection and Profiling of Erythrocyte-Derived Microvesicles","volume":"7","author":"Rho","year":"2013","journal-title":"Acs Nano"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.bios.2019.01.067","article-title":"Oscillatory flow-assisted efficient target enrichment with small volumes of sample by using a particle-based microarray device","volume":"131","author":"Lee","year":"2019","journal-title":"Biosens. Bioelectron."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"5282","DOI":"10.1039\/c2lc40065k","article-title":"SSA-MOA: A novel CTC isolation platform using selective size amplification (SSA) and a multi-obstacle architecture (MOA) filter","volume":"12","author":"Kim","year":"2012","journal-title":"Lab. Chip."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"18392","DOI":"10.1073\/pnas.1012539107","article-title":"Isolation of circulating tumor cells using a microvortex-generating herringbone-chip","volume":"107","author":"Stott","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"4895","DOI":"10.1002\/smll.201400719","article-title":"A Radial Flow Microfluidic Device for Ultra-High-Throughput Affinity-Based Isolation of Circulating Tumor Cells","volume":"10","author":"Murlidhar","year":"2014","journal-title":"Small"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"14280","DOI":"10.1039\/C8NR02871K","article-title":"Immuno-modified superparamagnetic nanoparticles via host-guest interactions for high-purity capture and mild release of exosomes","volume":"10","author":"Cai","year":"2018","journal-title":"Nanoscale"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4598","DOI":"10.1039\/C5LC01014D","article-title":"Separation of cancer cells from white blood cells by pinched flow fractionation","volume":"15","author":"Podenphant","year":"2015","journal-title":"Lab. Chip."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"9907","DOI":"10.1038\/s41598-017-08826-w","article-title":"Separation of extracellular nanovesicles and apoptotic bodies from cancer cell culture broth using tunable microfluidic systems","volume":"7","author":"Shin","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2321","DOI":"10.1021\/nn506538f","article-title":"Acoustic Purification of Extracellular Microvesicles","volume":"9","author":"Lee","year":"2015","journal-title":"Acs Nano"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4970","DOI":"10.1073\/pnas.1504484112","article-title":"Acoustic separation of circulating tumor cells","volume":"112","author":"Li","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"46224","DOI":"10.1038\/srep46224","article-title":"An integrated double-filtration microfluidic device for isolation, enrichment and quantification of urinary extracellular vesicles for detection of bladder cancer","volume":"7","author":"Liang","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.1021\/acsnano.6b06131","article-title":"Exodisc for Rapid, Size-Selective, and Efficient Isolation and Analysis of Nanoscale Extracellular Vesicles from Biological Samples","volume":"11","author":"Woo","year":"2017","journal-title":"ACS Nano"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"37673","DOI":"10.1038\/srep37673","article-title":"Nano sand filter with functionalized nanoparticles embedded in anodic aluminum oxide templates","volume":"6","author":"Phuong","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s10544-018-0319-z","article-title":"Clogging-free continuous operation with whole blood in a radial pillar device (RAPID)","volume":"20","author":"Mehendale","year":"2018","journal-title":"Biomed. Microdevices"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1038\/nnano.2016.134","article-title":"Nanoscale lateral displacement arrays for the separation of exosomes and colloids down to 20 nm","volume":"11","author":"Wunsch","year":"2016","journal-title":"Nat. Nanotechnol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"15167","DOI":"10.1038\/srep15167","article-title":"On-chip Extraction of Intracellular Molecules in White Blood Cells from Whole Blood","volume":"5","author":"Choi","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6138","DOI":"10.1038\/s41598-020-62937-5","article-title":"Nanofabrication of Isoporous Membranes for Cell Fractionation","volume":"10","author":"Sabirova","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4427","DOI":"10.1039\/D0NA00142B","article-title":"Use of nanosphere self-assembly to pattern nanoporous membranes for the study of extracellular vesicles","volume":"2","author":"Mireles","year":"2020","journal-title":"Nanoscale Adv."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1007\/s12195-014-0354-7","article-title":"Porous Membranes Promote Endothelial Differentiation of Adipose-Derived Stem Cells and Perivascular Interactions","volume":"7","author":"Mazzocchi","year":"2014","journal-title":"Cell. Mol. Bioeng."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.cherd.2020.07.027","article-title":"Dead-end and crossflow ultrafiltration process modelling: Application on chemical mechanical polishing wastewaters (vol 158, pg 164, 2020)","volume":"162","author":"Ohanessian","year":"2020","journal-title":"Chem. Eng. Res. Des."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1038\/s41598-020-57649-9","article-title":"Optimizing Flux Capacity of Dead-end Filtration Membranes by Controlling Flow with Pulse Width Modulated Periodic Backflush","volume":"10","author":"Enten","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1038\/nprot.2014.044","article-title":"Microfluidic, marker-free isolation of circulating tumor cells from blood samples","volume":"9","author":"Karabacak","year":"2014","journal-title":"Nat. Protoc."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"118101","DOI":"10.1103\/PhysRevLett.113.118101","article-title":"Volumetric Deformation of Live Cells Induced by Pressure-Activated Cross-Membrane Ion Transport","volume":"113","author":"Hui","year":"2014","journal-title":"Phys. Rev. Lett."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"179","DOI":"10.14356\/kona.2016024","article-title":"Developments of Blocking Filtration Model in Membrane Filtration","volume":"33","author":"Iritani","year":"2016","journal-title":"Kona Powder Part J."},{"key":"ref_27","first-page":"36","article-title":"Image Processing with ImageJ","volume":"11","author":"Ram","year":"2004","journal-title":"Biophotonics Int."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.memsci.2010.07.017","article-title":"Corn syrup clarification by microfiltration with ceramic membranes","volume":"363","author":"Almandoz","year":"2010","journal-title":"J. Membrane Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1007\/s11427-012-4399-3","article-title":"Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells","volume":"55","author":"Li","year":"2012","journal-title":"Sci. China Life Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1039\/C4LC01150C","article-title":"Microfluidic cellular enrichment and separation through differences in viscoelastic deformation","volume":"15","author":"Wang","year":"2015","journal-title":"Lab. Chip."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s10856-016-5775-5","article-title":"Elastic and viscoelastic mechanical properties of brain tissues on the implanting trajectory of sub-thalamic nucleus stimulation","volume":"27","author":"Li","year":"2016","journal-title":"J. Mater. Sci.-Mater. Med."},{"key":"ref_32","unstructured":"(2023, April 05). Available online: https:\/\/www.memsnet.org\/material\/siliconnitridesi3n4film\/."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"104263","DOI":"10.1016\/j.mechmat.2022.104263","article-title":"Finite element simulation of a viscoelastic cell entering a cylindrical channel: Effects of frictional contact","volume":"167","author":"Sun","year":"2022","journal-title":"Mech. Mater."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1021\/ie50306a024","article-title":"Studies in Filtration III. Derivation of General Filtration Equations","volume":"27","author":"Ruth","year":"1935","journal-title":"Ind. Eng. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1021\/ie50438a010","article-title":"Correlating Filtration Theory with Industrial Practice","volume":"38","author":"Ruth","year":"1946","journal-title":"Ind. Eng. Chem."},{"key":"ref_36","first-page":"1","article-title":"Principles of the mathematical treatment of constant-pressure filtration","volume":"55","author":"Hermans","year":"1936","journal-title":"J. Soc. Chem. Ind."},{"key":"ref_37","first-page":"303","article-title":"Resistance and Compressibility of Filter Cake. Chemical Engineering Progress","volume":"49","author":"Grace","year":"1953","journal-title":"Chem. Eng. Prog."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1002\/aic.690020308","article-title":"Structure and performance of filter media. II. Performance of filter media in liquid service","volume":"2","author":"Grace","year":"1956","journal-title":"AIChE J."},{"key":"ref_39","first-page":"183","article-title":"Constant pressure blocking filtration laws\u2014Application to power-law non-newtonian fluids","volume":"60","author":"Hermia","year":"1982","journal-title":"Trans. Inst. Chem. Eng."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1149\/1.2133542","article-title":"The loading effect in plasma etching","volume":"124","author":"Mogab","year":"1977","journal-title":"J. Electrochem. Soc."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1021\/nl052370x","article-title":"Suspended nanoporous membranes as interfaces for neuronal biohybrid systems","volume":"6","author":"Wolfrum","year":"2006","journal-title":"Nano Lett."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"107550","DOI":"10.1016\/j.foodcont.2020.107550","article-title":"Particle size analysis of pristine food-grade titanium dioxide and E 171 in confectionery products: Interlaboratory testing of a single-particle inductively coupled plasma mass spectrometry screening method and confirmation with transmission electron microscopy","volume":"120","author":"Geiss","year":"2021","journal-title":"Food Control"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3365","DOI":"10.1007\/s13201-016-0483-3","article-title":"Towards sustainable membrane filtration of palm oil mill effluent: Analysis of fouling phenomena from a hybrid PAC-UF process","volume":"7","author":"Amosa","year":"2017","journal-title":"Appl. Water Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1163\/156855206775992292","article-title":"Effect of particle size on the performance of cross-flow microfiltration","volume":"17","author":"Hwang","year":"2006","journal-title":"Adv. Powder Technol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1038\/nm0596-561","article-title":"Effects of a selective inhibitor of the Abl tyrosine kinase on the growth of Bcr-Abl positive cells","volume":"2","author":"Druker","year":"1996","journal-title":"Nat. Med."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1145\/3065386","article-title":"ImageNet Classification with Deep Convolutional Neural Networks","volume":"60","author":"Krizhevsky","year":"2017","journal-title":"Commun. ACM"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2278","DOI":"10.1109\/5.726791","article-title":"Gradient-based learning applied to document recognition","volume":"86","author":"Lecun","year":"1998","journal-title":"Proc. IEEE"},{"key":"ref_48","unstructured":"(ABAQUS Analysis User\u2019s Manual, 2006). ABAQUS Analysis User\u2019s Manual, Version 6.6."}],"container-title":["Membranes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0375\/13\/8\/707\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:22:05Z","timestamp":1760127725000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0375\/13\/8\/707"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,29]]},"references-count":48,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["membranes13080707"],"URL":"https:\/\/doi.org\/10.3390\/membranes13080707","relation":{},"ISSN":["2077-0375"],"issn-type":[{"value":"2077-0375","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,29]]}}}