{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T08:37:10Z","timestamp":1787733430409,"version":"build-2784847793"},"reference-count":60,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Membranes"],"abstract":"<jats:p>Interrelations between the plasma membrane and cytoskeleton are of crucial importance for essential cellular processes such as endocytosis, formation of intercellular junctions, cell morphology, etc. Many studies validate the beneficial effects of polyphenols as antioxidant and protective agents, but a molecular mechanism of their interaction and transition through the plasma membranes of different cell lines is still missing. In this study, we examined the affinity of fractions enriched in flavonoid glycosides (FGs) and caffeoylquinic acids (CQAs), obtained from the methanol extract of the medicinal plant Inula oculus-christi L., to reorganize the plasma membrane structure and actin cytoskeleton by using confocal microscopy. Assessment of the degree of membrane ordering aiming to distinguish the ordered from disordered regions of the cellular membranes was performed using the fluorescent dye Di-4-ANEPPDHQ, and visualization of F-actin was by TRITC-phalloidin. Two epithelial cell lines with clear differences in their origin and plasma membrane organization were chosen: the non-malignant MDCK II and the cancerous A549. Our results showed that flavonoid glycosides exhibited an ordering effect on plasma membranes of cancerous cells and fluidized one on non-malignant cells. Different patterns of actin reorganization were observed for both cell lines after treatment. Our results indicate the potential of plant-derived polyphenols as modulators of the membrane\u2019s structural organization, offering valuable insights for the development of membrane-targeted therapeutic strategies.<\/jats:p>","DOI":"10.3390\/membranes15120357","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T11:50:13Z","timestamp":1764244213000},"page":"357","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Polyphenols from Inula oculus-christi L. Induced Cell-Specific Membrane and Cytoskeleton Reorganization"],"prefix":"10.3390","volume":"15","author":[{"given":"Ralitsa","family":"Veleva","sequence":"first","affiliation":[{"name":"Faculty of Biology, Sofia University \u2018St. Kliment Ohridski\u2019, 8 Dragan Tzankov Blvd., 1164 Sofia, Bulgaria"},{"name":"Centre of Competence \u201cSustainable Utilization of Bio-resources and Waste of Medicinal and Aromatic Plants for Innovative Bioactive Products\u201d (BIORESOURCES BG), 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8097-3608","authenticated-orcid":false,"given":"Aneliya","family":"Kostadinova","sequence":"additional","affiliation":[{"name":"Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5273-176X","authenticated-orcid":false,"given":"Antoaneta","family":"Trendafilova","sequence":"additional","affiliation":[{"name":"Centre of Competence \u201cSustainable Utilization of Bio-resources and Waste of Medicinal and Aromatic Plants for Innovative Bioactive Products\u201d (BIORESOURCES BG), 1000 Sofia, Bulgaria"},{"name":"Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9056-0333","authenticated-orcid":false,"given":"Viktoria","family":"Ivanova","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5316-5835","authenticated-orcid":false,"given":"Veselina","family":"Moskova-Doumanova","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Sofia University \u2018St. Kliment Ohridski\u2019, 8 Dragan Tzankov Blvd., 1164 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirilka","family":"Mladenova","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Sofia University \u2018St. Kliment Ohridski\u2019, 8 Dragan Tzankov Blvd., 1164 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan","family":"Doumanov","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Sofia University \u2018St. Kliment Ohridski\u2019, 8 Dragan Tzankov Blvd., 1164 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayana","family":"Benkova","sequence":"additional","affiliation":[{"name":"Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. 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Ethnopharmacol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"114125","DOI":"10.1016\/j.jep.2021.114125","article-title":"A review of the botany, traditional uses, phytochemistry, and pharmacology of the Flos Inulae","volume":"276","author":"Yang","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Spiegel, M., Kapusta, K., Ko\u0142odziejczyk, W., Saloni, J., \u017bbikowska, B., Hill, G.A., and Sroka, Z. (2020). Antioxidant activity of selected phenolic acids\u2014Ferric reducing antioxidant power assay and QSAR analysis of the structural features. Molecules, 25.","DOI":"10.3390\/molecules25133088"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Rogozinska, M., Lisiecki, K., Czarnocki, Z., and Biesaga, M. (2023). Antioxidant activity of sulfate metabolites of chlorogenic acid. Appl. Sci., 13.","DOI":"10.3390\/app13042192"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s44372-025-00222-3","article-title":"Evaluation of total phenolic, flavonoid contents, antioxidant and antibacterial activity of leaf extracts from Rhus vulgaris","volume":"2","author":"Belew","year":"2025","journal-title":"Discov. Plants"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Xie, L., Deng, Z., Zhang, J., Dong, H., Wang, W., Xing, B., and Liu, X. (2022). Comparison of flavonoid O-glycoside, C-glycoside and their aglycones on antioxidant capacity and metabolism during in vitro digestion and in vivo. Foods, 11.","DOI":"10.3390\/foods11060882"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.3390\/microbiolres15040140","article-title":"Antibacterial and antioxidant activities of flavonoids, phenolic and flavonoid glycosides from Gouania longispicata leaves","volume":"15","author":"Gumisiriza","year":"2024","journal-title":"Microbiol. Res."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Ghozzi, I., Fontaine, J.-X., Molini\u00e9, R., Elboutachfaiti, R., Akkouche, L., Sebei, K., Mathiron, D., Hano, C., Garros, L., and Choque, E. (2024). Relationship between the structure of the flavone C-glycosides of linseed (Linum usitatissimum L.) and their antioxidant activity. Molecules, 29.","DOI":"10.3390\/molecules29245829"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Xie, J., Xiong, S., Li, Y., Xia, B., Li, M., Zhang, Z., Shi, Z., Peng, Q., Li, C., and Lin, L. (2024). Phenolic acids from medicinal and edible homologous plants: A potential anti-inflammatory agent for inflammatory diseases. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1345002"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"43243","DOI":"10.1021\/acsomega.3c06450","article-title":"Potential utilization of phenolic acid compounds as anti-inflammatory agents through TNF-\u03b1 convertase inhibition mechanisms: A network pharmacology, docking, and molecular dynamics approach","volume":"8","author":"Ekowati","year":"2023","journal-title":"ACS Omega"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"106812","DOI":"10.1016\/j.phrs.2023.106812","article-title":"Phenolic metabolites as therapeutic in inflammation and neoplasms: Molecular pathways explaining their efficacy","volume":"193","author":"Liu","year":"2023","journal-title":"Pharmacol. Res."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Wu, J., Huang, H., Gong, L., Tian, X., Peng, Z., Zhu, Y., and Wang, W. (2024). A flavonoid glycoside compound from Siraitia grosvenorii with anti-inflammatory and hepatoprotective effects in vitro. Biomolecules, 14.","DOI":"10.3390\/biom14040450"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"125124","DOI":"10.1016\/j.foodchem.2019.125124","article-title":"Anti-inflammatory effects of flavonoids","volume":"299","author":"Maleki","year":"2019","journal-title":"Food Chem."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Zhukov, A., and Vereshchagin, M. (2024). Polar glycerolipids and membrane lipid rafts. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25158325"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"27115","DOI":"10.1039\/D1RA02692E","article-title":"Role of polyunsaturated phospholipids in liquid-ordered and liquid-disordered phases","volume":"11","author":"Yang","year":"2021","journal-title":"RSC Adv."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1194\/jlr.E600002-JLR200","article-title":"Rafts defined: A report on the Keystone Symposium on lipid rafts and cell function","volume":"47","author":"Pike","year":"2006","journal-title":"J. Lipid Res."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Kusumi, A., Tsunoyama, T.A., Tang, B., Hirosawa, K.M., Morone, N., Fujiwara, T.K., and Suzuki, K.G.N. (2023). Cholesterol- and actin-centered view of the plasma membrane: Updating the Singer\u2013Nicolson fluid mosaic model to commemorate its 50th anniversary. Mol. Biol. Cell, 34.","DOI":"10.1091\/mbc.E20-12-0809"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"17221","DOI":"10.1074\/jbc.R000005200","article-title":"Structure and function of sphingolipid- and cholesterol-rich membrane rafts","volume":"275","author":"Brown","year":"2000","journal-title":"J. Biol. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/S0092-8674(04)00167-9","article-title":"Nanoscale organization of multiple GPI-anchored proteins in living cell membranes","volume":"116","author":"Sharma","year":"2004","journal-title":"Cell"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Trybus, M., Hryniewicz-Jankowska, A., W\u00f3jtowicz, K., Trombik, T., Czogalla, A., and Sikorski, A.F. (2023). EFR3A: A new raft domain organizing protein?. Cell. Mol. Biol. Lett., 28.","DOI":"10.1186\/s11658-023-00497-y"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"RP89306","DOI":"10.7554\/eLife.89306.3","article-title":"Partitioning to ordered membrane domains regulates the kinetics of secretory traffic","volume":"12","author":"Heberle","year":"2024","journal-title":"eLife"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Lietha, D., and Izard, T. (2020). Roles of membrane domains in integrin-mediated cell adhesion. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21155531"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e2302320120","DOI":"10.1073\/pnas.2302320120","article-title":"Lipid rafts, Rab GTPases, and a late endosomal checkpoint for plasma membrane recycling","volume":"120","author":"Daumann","year":"2023","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2319","DOI":"10.1007\/s00018-009-0022-6","article-title":"Cytoskeleton-membrane interactions in membrane raft structure","volume":"66","author":"Chichili","year":"2009","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4064","DOI":"10.1529\/biophysj.106.090852","article-title":"Actin polymerization serves as a membrane domain switch in model lipid bilayers","volume":"91","author":"Liu","year":"2006","journal-title":"Biophys. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1016\/j.bbamem.2013.07.018","article-title":"Interaction of membrane\/lipid rafts with the cytoskeleton: Impact on signaling and function: Membrane\/lipid rafts, mediators of cytoskeletal arrangement and cell signaling","volume":"1838","author":"Head","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1186\/s12944-025-02563-0","article-title":"Lipid rafts: Novel therapeutic targets for metabolic, neurodegenerative, oncological, and cardiovascular diseases","volume":"24","author":"Warda","year":"2025","journal-title":"Lipids Health Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1738","DOI":"10.1016\/j.cell.2019.04.037","article-title":"Integrin mechano-chemical signaling generates plasma membrane nanodomains that promote cell spreading","volume":"177","author":"Kalappurakkal","year":"2019","journal-title":"Cell"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Veiko, A.G., Sekowski, S., Lapshina, E.A., Wilczewska, A.Z., Markiewicz, K.H., Zamaraeva, M., Zhao, H., and Zavodnik, I.B. (2020). Flavonoids modulate liposomal membrane structure, regulate mitochondrial membrane permeability and prevent erythrocyte oxidative damage. Biochim. Biophys. Acta Biomembr., 1862.","DOI":"10.1016\/j.bbamem.2020.183442"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1016\/j.bbamem.2014.01.021","article-title":"Flavonoid\u2013Membrane Interactions: Involvement of Flavonoid\u2013Metal Complexes in Raft Signaling","volume":"1838","author":"Tarahovsky","year":"2014","journal-title":"Biochim. Biophys. Acta Biomembr."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3872","DOI":"10.1021\/acs.jafc.2c00444","article-title":"Targeting lipid rafts as a rapid screening strategy for potential antiadipogenic polyphenols along with the structure-activity relationship and mechanism elucidation","volume":"70","author":"Wang","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.bmcl.2016.12.051","article-title":"Inhibitory potential of flavonoids on PtdIns(3,4,5)P3 binding with the phosphoinositide-dependent kinase 1 pleckstrin homology domain","volume":"27","author":"Kanga","year":"2017","journal-title":"Bioorganic Med. Chem. Lett."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"19845","DOI":"10.1021\/acs.jafc.3c06747","article-title":"Insights into the surface binding and structural interference of polyphenols with the membrane raft domains in relation to their distinctive ability to inhibit preadipocyte differentiation in 3T3-L1 cells","volume":"71","author":"Wang","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.jare.2023.04.017","article-title":"Antimicrobial activities of lavandulylated flavonoids in Sophora flavences against methicillin-resistant Staphylococcus aureus via membrane disruption","volume":"57","author":"Weng","year":"2024","journal-title":"J. Adv. Res."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Veleva, R., Topouzova-Hristova, T., Kostadinova, A., Benkova, D., Trendafilova, A., Ivanova, V., Moskova-Doumanova, V., Mladenova, K., Doumanov, J., and Yordanova, V. (2025). Flavonoid Glycosides and Phenolic Acids from Inula oculus-christi Modulate Membrane Organization and Provide Antioxidant Protection. Molecules, 30.","DOI":"10.3390\/molecules30132740"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Trendafilova, A., Ivanova, V., Rangelov, M., Todorova, M., Ozek, G., Yur, S., Ozek, T., Aneva, I., Veleva, R., and Moskova-Doumanova, V. (2020). Caffeoylquinic acids, cytotoxic, antioxidant, acetylcholinesterase and tyrosinase enzyme inhibitory activities of six Inula species from Bulgaria. Chem Biodivers., 17.","DOI":"10.1002\/cbdv.202000051"},{"key":"ref_37","first-page":"176","article-title":"Phenolic constituents and antioxidant capacity of Inula oculus-christi from Bulgaria","volume":"49","author":"Trendafilova","year":"2017","journal-title":"Bulg. Chem. Commun."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Kostadinova, A., Staneva, G., Topouzova-Hristova, T., Moyankova, D., Yordanova, V., Veleva, R., Nikolova, B., Momchilova, A., Djilianov, D., and Hazarosova, R. (2022). Myconoside Affects the Viability of Polarized Epithelial MDCKII Cell Line by Interacting with the Plasma Membrane and the Apical Junctional Complexes. Separations, 9.","DOI":"10.3390\/separations9090239"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/nprot.2011.419","article-title":"Quantitative imaging of membrane lipid order in cells and organisms","volume":"7","author":"Owen","year":"2011","journal-title":"Nat Protoc."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1016\/j.biochi.2011.12.015","article-title":"Alkyl esters of hydroxycinnamic acids with improved antioxidant activity and lipophilicity protect PC12 cells against oxidative stress","volume":"94","author":"Garrido","year":"2012","journal-title":"Biochimie"},{"key":"ref_41","first-page":"1","article-title":"Computational study of natural phenolic acid solubility and their interactions with chitosan","volume":"2","author":"Delmondes","year":"2017","journal-title":"MOL2NET"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.tifs.2018.07.006","article-title":"New insights on bioactivities and biosynthesis of flavonoid glycosides","volume":"79","author":"Yang","year":"2018","journal-title":"Trends Food Sci. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.abb.2007.02.003","article-title":"Structure\u2013activity relationships of flavonoid-induced cytotoxicity on human leukemia cells","volume":"460","author":"Plochmann","year":"2007","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Kalinowska, M., \u015awis\u0142ocka, R., Wo\u0142ejko, E., Jab\u0142o\u0144ska-Trypu\u0107, A., Wydro, U., Koz\u0142owski, M., Koronkiewicz, K., Piekut, J., and Lewandowski, W. (2024). Structural characterization and evaluation of antimicrobial and cytotoxic activity of six plant phenolic acids. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0299372"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Chang, C.-J., Hung, Y.-L., Chen, T.-C., Li, H.-J., Lo, Y.-H., Wu, N.-L., Chang, D.-C., and Hung, C.-F. (2021). Anti-proliferative and anti-migratory activities of hispidulin on human melanoma A2058 cells. Biomolecules, 11.","DOI":"10.3390\/biom11071039"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"717","DOI":"10.3892\/or.2020.7891","article-title":"Chlorogenic acid induces apoptosis, inhibits metastasis and improves antitumor immunity in breast cancer via the NF-\u03baB signaling pathway","volume":"45","author":"Zeng","year":"2021","journal-title":"Oncol. Rep."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Yeager, A.N., Weber, P.K., and Kraft, M.L. (2016). Three-dimensional imaging of cholesterol and sphingolipids within a Madin\u2013Darby canine kidney cell. Biointerphases, 11.","DOI":"10.1116\/1.4939681"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1158\/0008-5472.CAN-14-3216","article-title":"Cell membrane fluid\u2013mosaic structure and cancer metastasis","volume":"75","author":"Nicolson","year":"2015","journal-title":"Cancer Res."},{"key":"ref_49","first-page":"50","article-title":"Disturbing cytoskeleton by engineered nanomaterials for enhanced cancer therapeutics","volume":"29","author":"Xu","year":"2023","journal-title":"Bioact. Mater."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Nurmagambetova, A., Mustyatsa, V., Saidova, A., and Vorobjev, I. (2023). Morphological and cytoskeleton changes in cells after EMT. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-48279-y"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/s40495-020-00242-5","article-title":"Are compounds membrane-associated or present in the cytosol? A study using polyphenols in a colon carcinoma cell line model","volume":"6","author":"Deusser","year":"2020","journal-title":"Curr. Pharmacol. Rep."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.plipres.2014.11.002","article-title":"Influence of membrane lipid composition on flavonoid\u2013membrane interactions: Implications on their biological activity","volume":"58","author":"Selvaraj","year":"2015","journal-title":"Prog. Lipid Res."},{"key":"ref_53","first-page":"19","article-title":"Flavonoid\u2013membrane interactions: A protective role of flavonoids at the membrane surface?","volume":"12","author":"Oteiza","year":"2005","journal-title":"Clin. Dev. Immunol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"46","DOI":"10.3109\/09687688.2015.1031833","article-title":"Molecular mechanism of action of chlorogenic acid on erythrocyte and lipid membranes","volume":"32","year":"2015","journal-title":"Mol. Membr. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.freeradbiomed.2017.12.002","article-title":"Differential targeting of membrane lipid domains by caffeic acid and its ester derivatives","volume":"115","author":"Filipe","year":"2018","journal-title":"Free Radic. Biol. Med."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Carrano, R., Grande, M., Leti Maggio, E., Zucca, C., Bei, R., Palumbo, C., Focaccetti, C., Nardozi, D., Lucarini, V., and Angiolini, V. (2024). Dietary polyphenols effects on focal adhesion plaques and metalloproteinases in cancer invasiveness. Biomedicines, 12.","DOI":"10.3390\/biomedicines12030482"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Wang, R., Peng, J., Shi, X., Cao, S., Xu, Y., Xiao, G., and Li, C. (2022). Change in membrane fluidity induced by polyphenols is highly dependent on the position and number of galloyl groups. Biochim. Biophys. Acta Biomembr., 1864.","DOI":"10.1016\/j.bbamem.2022.184015"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Zhou, A., Xue, B., Zhong, J., Liu, J., Peng, R., Wang, F., Zhou, Y., Tang, J., Yang, Q., and Chen, X. (2025). Cholesterol-rich lipid rafts mediate endocytosis as a common pathway for respiratory syncytial virus entry into different host cells. Microbiol. Spectr., 13.","DOI":"10.1128\/spectrum.01192-25"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41392-022-01259-6","article-title":"Targeting integrin pathways: Mechanisms and advances in therapy","volume":"8","author":"Pang","year":"2023","journal-title":"Signal Transduct. Target Ther."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Hanna, S.J., McCoy-Simandle, K., Miskolci, V., Guo, P., Cammaer, M., Hodgson, L., and Cox, D. (2017). The role of Rho-GTPases and actin polymerization during macrophage tunneling nanotube biogenesis. Sci. Rep., 7.","DOI":"10.1038\/s41598-017-08950-7"}],"container-title":["Membranes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0375\/15\/12\/357\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T09:05:29Z","timestamp":1764752729000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0375\/15\/12\/357"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,26]]},"references-count":60,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["membranes15120357"],"URL":"https:\/\/doi.org\/10.3390\/membranes15120357","relation":{},"ISSN":["2077-0375"],"issn-type":[{"value":"2077-0375","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,26]]}}}