{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:16:15Z","timestamp":1785428175724,"version":"3.56.0"},"reference-count":26,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T00:00:00Z","timestamp":1767139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Membranes"],"abstract":"<jats:p>Chronic and complex wounds require biomaterials that are both cytocompatible and antimicrobial. Herein, electrospun polycaprolactone (PCL) nanofiber membranes were coated with Type I collagen and functionalized with silver nanoparticles (AgNPs). The main objective was to assess fibroblast adhesion, proliferation, and cytotoxicity. Membrane morphology and surface characteristics were analyzed in a previous work by SEM, AFM, and wettability measurements, confirming the transformation from hydrophobic PCL to fully wettable collagen-coated surfaces. In this study, Murine 3T3 fibroblasts were cultured on PCL, PCL\u2013Collagen, PCL\u2013Collagen\u2013Citrate, and PCL\u2013Collagen\u2013AgNPs membranes. Cellular activity was quantified using Alamar Blue assays at 24, 48, and 72 h, while cytotoxicity was determined by LDH release. Cellular viability and adhesion were studied using confocal microscopy. All membrane types supported fibroblast growth, with collagen-coated samples exhibiting the highest metabolic activity. AgNPs-functionalized membranes sustained overall cell viability above 90%, with cytotoxicity values of approximately 10% at 24 h and 20% at 48 h. Antimicrobial evaluations demonstrated complete inhibition of Pseudomonas aeruginosa and vancomycin-resistant Enterococcus, and partial inhibition of Staphylococcus aureus. These results indicate that collagen-coated, AgNPs-functionalized electrospun PCL membranes exhibit both high cytocompatibility and significant antimicrobial activity, supporting their potential as advanced wound-dressing materials.<\/jats:p>","DOI":"10.3390\/membranes16010017","type":"journal-article","created":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T16:34:23Z","timestamp":1767198863000},"page":"17","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Cell Adhesion and Cytotoxicity Assessment of Collagen-Coated Electrospun PCL Membranes Functionalized with Silver Nanoparticles"],"prefix":"10.3390","volume":"16","author":[{"given":"Chiara","family":"Goisis","sequence":"first","affiliation":[{"name":"Plastic and Reconstructive Surgery Unit, Cattinara Hospital, Strada di Fiume, 447, 34149 Trieste, Italy"},{"name":"Clinical Department of Medical, Surgical and Health Sciences, University of Trieste, Strada di Fiume, 447, 34149 Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6437-7646","authenticated-orcid":false,"given":"Davide","family":"Porrelli","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Trieste, Via Alexander Fleming 31\/B, 34127 Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5699-2131","authenticated-orcid":false,"given":"Gianluca","family":"Turco","sequence":"additional","affiliation":[{"name":"Clinical Department of Medical, Surgical and Health Sciences, University of Trieste, Piazza Dell\u2019Ospitale 1, 34129 Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0038-1782","authenticated-orcid":false,"given":"Barbara","family":"Medagli","sequence":"additional","affiliation":[{"name":"Clinical Department of Medical, Surgical and Health Sciences, University of Trieste, Piazza Dell\u2019Ospitale 1, 34129 Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4676-2775","authenticated-orcid":false,"given":"Giovanni","family":"Papa","sequence":"additional","affiliation":[{"name":"Plastic and Reconstructive Surgery Unit, Cattinara Hospital, Strada di Fiume, 447, 34149 Trieste, Italy"},{"name":"Clinical Department of Medical, Surgical and Health Sciences, University of Trieste, Strada di Fiume, 447, 34149 Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4634-2582","authenticated-orcid":false,"given":"Martin","family":"Iurilli","sequence":"additional","affiliation":[{"name":"Plastic and Reconstructive Surgery Unit, Cattinara Hospital, Strada di Fiume, 447, 34149 Trieste, Italy"},{"name":"Clinical Department of Medical, Surgical and Health Sciences, University of Trieste, Strada di Fiume, 447, 34149 Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1111\/bjd.13954","article-title":"Cellular and molecular mechanisms of repair in acute and chronic wound healing","volume":"173","author":"Martin","year":"2015","journal-title":"Br. 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