{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T07:43:26Z","timestamp":1776757406319,"version":"3.51.2"},"reference-count":80,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,8,9]],"date-time":"2020-08-09T00:00:00Z","timestamp":1596931200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Charles University Prague","award":["Progres Q40\/02"],"award-info":[{"award-number":["Progres Q40\/02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>In hyperammonemic states, such as liver cirrhosis, urea cycle disorders, and strenuous exercise, the catabolism of branched-chain amino acids (BCAAs; leucine, isoleucine, and valine) is activated and BCAA concentrations decrease. In these conditions, BCAAs are recommended to improve mental functions, protein balance, and muscle performance. However, clinical trials have not demonstrated significant benefits of BCAA-containing supplements. It is hypothesized that, under hyperammonemic conditions, enhanced glutamine availability and decreased BCAA levels facilitate the amination of branched-chain keto acids (BCKAs; \u03b1-ketoisocaproate, \u03b1-keto-\u03b2-methylvalerate, and \u03b1-ketoisovalerate) to the corresponding BCAAs, and that BCKA supplementation may offer advantages over BCAAs. Studies examining the effects of ketoanalogues of amino acids have provided proof that subjects with hyperammonemia can effectively synthesize BCAAs from BCKAs. Unfortunately, the benefits of BCKA administration have not been clearly confirmed. The shortcoming of most reports is the use of mixtures intended for patients with renal insufficiency, which might be detrimental for patients with liver injury. It is concluded that (i) BCKA administration may decrease ammonia production, attenuate cataplerosis, correct amino acid imbalance, and improve protein balance and (ii) studies specifically investigating the effects of BCKA, without the interference of other ketoanalogues, are needed to complete the information essential for decisions regarding their suitability in hyperammonemic conditions.<\/jats:p>","DOI":"10.3390\/metabo10080324","type":"journal-article","created":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T07:25:03Z","timestamp":1597044303000},"page":"324","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Branched-Chain Amino Acids and Branched-Chain Keto Acids in Hyperammonemic States: Metabolism and as Supplements"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0385-8881","authenticated-orcid":false,"given":"Milan","family":"Hole\u010dek","sequence":"first","affiliation":[{"name":"Department of Physiology, Charles University, Faculty of Medicine in Hradec Kr\u00e1lov\u00e9, 500 03 Hradec Kralove, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S129","DOI":"10.1055\/s-2007-1024864","article-title":"Exercise-induced hyperammonemia: Peripheral and central effects","volume":"11","author":"Banister","year":"1990","journal-title":"Int. J. Sports Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.pneurobio.2010.01.012","article-title":"Ammonia metabolism, the brain and fatigue; revisiting the link","volume":"91","author":"Wilkinson","year":"2010","journal-title":"Prog. Neurobiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1139\/y92-020","article-title":"Ammonia and amino acid metabolism in human skeletal muscle during exercise","volume":"70","author":"Graham","year":"1992","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1016\/S0168-8278(96)80249-2","article-title":"Hyperammonemia-induced depletion of glutamate and branched-chain amino acids in muscle and plasma","volume":"25","author":"Leweling","year":"1996","journal-title":"J. Hepatol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1053\/meta.2000.9531","article-title":"Effect of hyperammonemia on leucine and protein metabolism in rats","volume":"49","year":"2000","journal-title":"Metabolism"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1007\/s00726-010-0679-z","article-title":"Acute hyperammonemia activates branched-chain amino acid catabolism and decreases their extracellular concentrations: Different sensitivity of red and white muscle","volume":"40","author":"Holecek","year":"2011","journal-title":"Amino Acids"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1007\/s13105-018-0646-9","article-title":"Effects of branched-chain amino acids on muscles under hyperammonemic conditions","volume":"74","year":"2018","journal-title":"J. Physiol. Biochem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0140-6736(71)92048-4","article-title":"False neurotransmitters and hepatic failure","volume":"298","author":"Fischer","year":"1971","journal-title":"Lancet"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"545","DOI":"10.7326\/0003-4819-93-4-545","article-title":"The use of ornithine salts of branched-chain ketoacids in portal-systemic encephalopathy","volume":"93","author":"Herlong","year":"1980","journal-title":"Ann. Intern. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/BF00807325","article-title":"Plasma amino acids in four models of experimental liver injury in rats","volume":"10","year":"1996","journal-title":"Amino Acids"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1111\/iep.12299","article-title":"Muscle wasting and branched-chain amino acid, alpha-ketoglutarate, and ATP depletion in a rat model of liver cirrhosis","volume":"99","year":"2018","journal-title":"Int. J. Exp. Pathol."},{"key":"ref_12","first-page":"227","article-title":"Long-term management of a case of carbamyl phosphate synthetase deficiency using ketanalogues and hydroxyanalogues of essential amino acids","volume":"58","author":"Batshaw","year":"1976","journal-title":"Pediatrics"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/8904_2012_186","article-title":"Amino acid profiles in patients with urea cycle disorders at admission to hospital due to metabolic decompensation","volume":"9","author":"Rodney","year":"2013","journal-title":"JIMD Rep."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Als-Nielsen, B., Koretz, R.L., Kjaergard, L.L., and Gluud, C. (2003). Branched-chain amino acids for hepatic encephalopathy. Cochrane Database Syst. Rev.","DOI":"10.1002\/14651858.CD001939"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Gluud, L.L., Dam, G., Les, I., C\u00f3rdoba, J., Marchesini, G., Borre, M., Aagaard, N.K., and Vilstrup, H. (2015). Branched-chain amino acids for people with hepatic encephalopathy. Cochrane Database Syst. Rev.","DOI":"10.1002\/14651858.CD001939.pub3"},{"key":"ref_16","first-page":"458","article-title":"Isotopic analysis of leucine and urea metabolism in exercising humans","volume":"52","author":"Wolfe","year":"1982","journal-title":"J. Appl. Physiol. Respir. Environ. Exerc. Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1152\/jappl.1991.70.1.43","article-title":"Leucine metabolism during fasting and exercise","volume":"70","author":"Knapik","year":"1991","journal-title":"J. Appl. Physiol. (1985)"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1249\/MSS.0b013e3182797637","article-title":"Caffeine decreases systemic urea in elite soccer players during intermittent exercise","volume":"45","author":"Bassini","year":"2013","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1007\/BF00805860","article-title":"Branched-chain\u03b1-amino acid chronic treatment: Responses of plasma \u03b1-keto-related compounds and ammonia when used in physical exercise performance","volume":"10","author":"Gatti","year":"1996","journal-title":"Amino Acids"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2644","DOI":"10.1152\/jappl.1996.81.6.2644","article-title":"Effects of glucose, glucose plus branched-chain amino acids, or placebo on bike performance over 100 km","volume":"81","author":"Madsen","year":"1996","journal-title":"J. Appl. Physiol. (1985)"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1113\/jphysiol.1996.sp021433","article-title":"Stimulation of muscle ammonia production during exercise following branched-chain amino acid supplementation in humans","volume":"493","author":"MacLean","year":"1996","journal-title":"J. Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1007\/s00421-004-1206-2","article-title":"The effect of acute branched-chain amino acid supplementation on prolonged exercise capacity in a warm environment","volume":"93","author":"Watson","year":"2004","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.3390\/nu4111767","article-title":"Effects of diets supplemented with branched-chain amino acids on the performance and fatigue mechanisms of rats submitted to prolonged physical exercise","volume":"4","author":"Falavigna","year":"2012","journal-title":"Nutrients"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"S101","DOI":"10.1055\/s-2007-1024861","article-title":"Metabolism of branched-chain amino acids and ammonia during exercise: Clues from McArdle\u2019s disease","volume":"11","author":"Wagenmakers","year":"1990","journal-title":"Int. J. Sports Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/s11011-013-9428-9","article-title":"Evidence of a vicious cycle in glutamine synthesis and breakdown in pathogenesis of hepatic encephalopathy-therapeutic perspectives","volume":"29","author":"Holecek","year":"2014","journal-title":"Metab. Brain Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.nut.2017.04.003","article-title":"Branched-chain amino acid supplementation in treatment of liver cirrhosis: Updated views on how to attenuate their harmful effects on cataplerosis and ammonia formation","volume":"41","year":"2017","journal-title":"Nutrition"},{"key":"ref_27","first-page":"321","article-title":"Effect of a branched chain amino acid-enriched nutritional product on the pathophysiology of the liver and nutritional state of patients with liver cirrhosis","volume":"37","author":"Watanabe","year":"1983","journal-title":"Acta Med. Okayama"},{"key":"ref_28","first-page":"25","article-title":"Administration of a branched-chain amino acid preparation during hepatic failure: A study emphasizing ammonia metabolism","volume":"48","author":"Nishikawa","year":"1994","journal-title":"Acta Med. Okayama"},{"key":"ref_29","first-page":"G269","article-title":"Branched-chain amino acids increase arterial blood ammonia in spite of enhanced intrinsic muscle ammonia metabolism in patients with cirrhosis and healthy subjects","volume":"301","author":"Dam","year":"2011","journal-title":"Am. J. Physiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1023\/A:1020757730386","article-title":"Effects of a branched-chain amino acid-enriched diet on chronic hepatic encephalopathy in dogs","volume":"14","author":"Meyer","year":"1999","journal-title":"Metab. Brain Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1159\/000169557","article-title":"Amino acids and keto acids in the treatment of chronic renal failure","volume":"6","author":"Jungers","year":"1988","journal-title":"Blood Purif."},{"key":"ref_32","first-page":"S285","article-title":"Supplements containing amino acids and keto acids in the treatment of chronic uremia","volume":"16","author":"Walser","year":"1983","journal-title":"Kidney Int. Suppl."},{"key":"ref_33","first-page":"876","article-title":"Effect of a keto acid-amino acid supplement on the metabolism and renal elimination of branched-chain amino acids in patients with chronic renal insufficiency on a low protein diet","volume":"112","author":"Teplan","year":"2000","journal-title":"Wien. Klin. Wochenschr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1146\/annurev.nu.04.070184.002205","article-title":"Branched-chain amino acid metabolism","volume":"4","author":"Harper","year":"1984","journal-title":"Annu. Rev. Nutr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1042\/cs0750301","article-title":"Utilization for protein synthesis of 2-ketoisocaproate relative to utilization of leucine, as estimated from exhalation of labelled CO2","volume":"75","author":"Imura","year":"1988","journal-title":"Clin. Sci. (Lond.)"},{"key":"ref_36","first-page":"E633","article-title":"Nitrogen sparing by 2-ketoisocaproate in parenterally fed rats","volume":"259","author":"Yagi","year":"1990","journal-title":"Am. J. Physiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"25","DOI":"10.33549\/physiolres.930022","article-title":"Metabolism of branched-chain amino acids in starved rats: The role of hepatic tissue","volume":"50","author":"Holecek","year":"2001","journal-title":"Physiol. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/S0899-9007(00)00483-4","article-title":"The BCAA-BCKA cycle: Its relation to alanine and glutamine synthesis and protein balance","volume":"17","author":"Holecek","year":"2001","journal-title":"Nutrition"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1016\/S0026-0495(03)00107-0","article-title":"Acute effects of decreased glutamine supply on protein and amino acid metabolism in hepatic tissue: A study using isolated perfused rat liver","volume":"52","author":"Holecek","year":"2003","journal-title":"Metabolism"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1002\/hep.1840060325","article-title":"Effect of experimental liver disease on the utilization for protein synthesis of orally administered alpha-ketoisocaproate","volume":"6","author":"Walser","year":"1986","journal-title":"Hepatology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2865","DOI":"10.1172\/JCI107483","article-title":"Synthesis of essential amino acids from their alpha-keto analogues by perfused rat liver and muscle","volume":"52","author":"Walser","year":"1973","journal-title":"J. Clin. Invest."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/0304-4165(81)90153-7","article-title":"Metabolism of 2-oxoacid analogues of leucine, valine and phenylalanine by heart muscle, brain and kidney of the rat","volume":"677","author":"Brand","year":"1981","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1016\/S0026-0495(98)90030-0","article-title":"Leucine metabolism in rat liver after a bolus injection of endotoxin","volume":"47","author":"Holecek","year":"1998","journal-title":"Metabolism"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1177\/0148607106030006467","article-title":"Simultaneous infusion of glutamine and branched-chain amino acids (BCAA) to septic rats does not have more favorable effect on protein synthesis in muscle, liver, and small intestine than separate infusions","volume":"30","year":"2006","journal-title":"JPEN J. Parenter. Enter. Nutr."},{"key":"ref_45","first-page":"E123","article-title":"Disposal of alpha-ketoisocaproate: Roles of liver, gut, and kidneys","volume":"243","author":"Abumrad","year":"1982","journal-title":"Am. J. Physiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1172\/JCI108671","article-title":"Distribution of branched-chain alpha-keto acid dehydrogenases in primate tissues","volume":"59","author":"Khatra","year":"1977","journal-title":"J. Clin. Investig."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1093\/jn\/114.7.1235","article-title":"Effects of branched-chain alpha-keto acids on plasma amino acid concentrations in carbon tetrachloride-intoxicated rats","volume":"114","author":"Okita","year":"1984","journal-title":"J. Nutr."},{"key":"ref_48","first-page":"701","article-title":"Pharmacokinetic and metabolic interrelationships among branched-chain keto and amino acids in humans","volume":"106","author":"Schauder","year":"1985","journal-title":"J. Lab. Clin. Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1172\/JCI110066","article-title":"Nitrogen sparing induced by leucine compared with that induced by its keto analogue, alpha-ketoisocaproate, in fasting obese man","volume":"67","author":"Mitch","year":"1981","journal-title":"J. Clin. Investig."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1016\/S0021-9258(19)68081-6","article-title":"Does leucine, leucyl-tRNA, or some metabolite of leucine regulate protein synthesis and degradation in skeletal and cardiac muscle?","volume":"257","author":"Tischler","year":"1982","journal-title":"J. Biol. Chem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1002\/mus.880050304","article-title":"Branched-chain ketoacids reduce muscle protein degradation in Duchenne muscular dystrophy","volume":"5","author":"Stewart","year":"1982","journal-title":"Muscle Nerve"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1016\/S0140-6736(83)92643-0","article-title":"Effects of alpha-ketoisocaproate and of leucine on nitrogen metabolism in postoperative patients","volume":"1","author":"Sapir","year":"1983","journal-title":"Lancet"},{"key":"ref_53","first-page":"30","article-title":"The effect of ketone bodies and dietary carbohydrate intake on protein metabolism","volume":"507","author":"Sherwin","year":"1981","journal-title":"Acta Chir. Scand. Suppl."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1002\/jcsm.12208","article-title":"Beta-hydroxy-beta-methylbutyrate supplementation and skeletal muscle in healthy and muscle-wasting conditions","volume":"8","year":"2017","journal-title":"J. Cachexia Sarcopenia Muscle"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/S0016-5085(76)80185-0","article-title":"Effects of keto analogues of essential amino acids in portal-systemic encephalopathy","volume":"71","author":"Maddrey","year":"1976","journal-title":"Gastroenterology"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1042\/cs0620285","article-title":"Intravenous infusion of alpha-oxoisocaproate: Influence on amino acid and nitrogen metabolism in patients with liver cirrhosis","volume":"62","author":"Eriksson","year":"1982","journal-title":"Clin. Sci. (Lond.)"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1159\/000198784","article-title":"Oral keto analogs of branched-chain amino acids in hyperammonemia in patients with cirrhosis of the liver. A double-blind crossover study","volume":"24","author":"Walker","year":"1982","journal-title":"Digestion"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/s12986-016-0072-3","article-title":"Alterations in protein and amino acid metabolism in rats fed a branched-chain amino acid- or leucine-enriched diet during postprandial and postabsorptive states","volume":"13","author":"Holecek","year":"2016","journal-title":"Nutr. Metab. (Lond.)"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"S79","DOI":"10.1016\/j.ymgme.2003.11.017","article-title":"Effect of alternative pathway therapy on branched chain amino acid metabolism in urea cycle disorder patients","volume":"81","author":"Scaglia","year":"2004","journal-title":"Mol. Genet. Metab."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1093\/hmg\/ddq507","article-title":"Phenylbutyrate therapy for maple syrup urine disease","volume":"20","author":"Lanpher","year":"2011","journal-title":"Hum. Mol. Genet."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1111\/iep.12190","article-title":"Phenylbutyrate exerts adverse effects on liver regeneration and amino acid concentrations in partially hepatectomized rats","volume":"97","author":"Holecek","year":"2016","journal-title":"Int. J. Exp. Pathol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1056\/NEJM197505222922101","article-title":"Treatment of carbamyl phosphate synthetase deficiency with keto analogues of essential amino acids","volume":"292","author":"Batshaw","year":"1975","journal-title":"N. Engl. J. Med."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/S0022-3476(77)80633-1","article-title":"Neonatal citrllinemia: Treatment with keto-analogues of essential amino acids","volume":"90","author":"Thoene","year":"1977","journal-title":"J. Pediatr."},{"key":"ref_64","first-page":"173","article-title":"Nitrogen metabolism in neonatal citrullinaemia","volume":"53","author":"Walser","year":"1977","journal-title":"Clin. Sci. Mol. Med."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1542\/peds.62.1.30","article-title":"Studies of the cause and treatment of hyperammonemia in females with ornithine transcarbamylase deficiency","volume":"62","author":"Glasgow","year":"1978","journal-title":"Pediatrics"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/S0022-3476(78)81149-4","article-title":"Treatment of complete ornithine transcarbamylase deficiency with nitrogen-free analogues of essential amino acids","volume":"93","author":"McReynolds","year":"1978","journal-title":"J. Pediatr."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"347","DOI":"10.2165\/00007256-199927060-00001","article-title":"Leucine supplementation and intensive training","volume":"27","author":"Mero","year":"1999","journal-title":"Sports Med."},{"key":"ref_68","first-page":"E170","article-title":"Ammonia metabolism during intense dynamic exercise and recovery in humans","volume":"259","author":"Graham","year":"1990","journal-title":"Am. J. Physiol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1111\/j.1469-7793.1999.0761p.x","article-title":"The effect of muscle contraction on the regulation of adenosine formation in rat skeletal muscle cells","volume":"518","author":"Hellsten","year":"1999","journal-title":"J. Physiol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"S1583","DOI":"10.1093\/jn\/134.6.1583S","article-title":"Exercise promotes BCAA catabolism: Effects of BCAA supplementation on skeletal muscle during exercise","volume":"134","author":"Shimomura","year":"2004","journal-title":"J. Nutr."},{"key":"ref_71","first-page":"10","article-title":"Is branched-chain amino acids supplementation an efficient nutritional strategy to alleviate skeletal muscle damage? A systematic review","volume":"9","author":"Bendahan","year":"2017","journal-title":"Nutrients"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1519\/JSC.0b013e3181bf443a","article-title":"Branched-chain amino acid supplementation lowers perceived exertion but does not affect performance in untrained males","volume":"25","author":"Greer","year":"2011","journal-title":"J. Strength Cond. Res."},{"key":"ref_73","first-page":"347","article-title":"Branched-chain amino acid supplementation does not enhance athletic performance but affects muscle recovery and the immune system","volume":"48","author":"Negro","year":"2008","journal-title":"J. Sports Med. Phys. Fitness"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/s12970-016-0142-y","article-title":"Post-exercise branched chain amino acid supplementation does not affect recovery markers following three consecutive high intensity resistance training bouts compared to carbohydrate supplementation","volume":"13","author":"Kephart","year":"2016","journal-title":"J. Int. Soc. Sports Nutr."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1438","DOI":"10.1017\/S0007114510002321","article-title":"Acute supplementation with keto analogues and amino acids in rats during resistance exercise","volume":"104","author":"Prado","year":"2010","journal-title":"Br. J. Nutr."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1729","DOI":"10.1017\/S000711451000557X","article-title":"Keto analogue and amino acid supplementation affects the ammonaemia response during exercise under ketogenic conditions","volume":"105","author":"Prado","year":"2011","journal-title":"Br. J. Nutr."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/1550-2783-9-37","article-title":"Improved training tolerance by supplementation with \u03b1-Keto acids in untrained young adults: A randomized, double blind, placebo-controlled trial","volume":"9","author":"Liu","year":"2012","journal-title":"J. Int. Soc. Sports Nutr."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"2224","DOI":"10.1039\/C5FO00263J","article-title":"The supportive effect of supplementation with \u03b1-keto acids on physical training in type 2 diabetes mellitus","volume":"6","author":"Liu","year":"2015","journal-title":"Food Funct."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1039\/C5FO01054C","article-title":"Keto analogue and amino acid supplementation and its effects on ammonemia and performance under thermoneutral conditions","volume":"7","author":"Camerino","year":"2016","journal-title":"Food Funct."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1039\/C7FO00189D","article-title":"Keto analogues and amino acids supplementation induces a decrease of white blood cell counts and a reduction of muscle damage during intense exercise under thermoneutral conditions","volume":"8","author":"Lima","year":"2017","journal-title":"Food Funct."}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/10\/8\/324\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:58:20Z","timestamp":1760176700000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/10\/8\/324"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,9]]},"references-count":80,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2020,8]]}},"alternative-id":["metabo10080324"],"URL":"https:\/\/doi.org\/10.3390\/metabo10080324","relation":{},"ISSN":["2218-1989"],"issn-type":[{"value":"2218-1989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,9]]}}}