{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:22:31Z","timestamp":1785403351321,"version":"3.56.0"},"reference-count":55,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T00:00:00Z","timestamp":1619654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Phagnalon saxatile subsp. saxatile is a wild species widespread in Algeria which is utilized for medicinal purposes as analgesic and anticholesterolemic. However, information is still scarce regarding its phytochemical content. The objective of this study was to identify and quantify the phenolic compounds from different extracts of its leafy stems. For this purpose, the effects of four extracting solvents were investigated on the content of phenolic compounds and the antioxidant activity of this plant. The extracts prepared with polar solvents (methanol and water) contained higher amounts of phenolic compounds and showed better antioxidant activity than the extracts with apolar solvents (hexane, dichloromethane). The methanolic extract, richest in total phenolic and total flavonoid, had significant antioxidant activity as regarded by DPPH\u00b0 scavenging capacity (IC50 of 5.5 \u00b5g\/mL), ABTS+\u00b0 scavenging capacity (IC50 of 63.8 \u00b5g\/mL) and inhibition of oxidation of linoleic acid (IC50 of 22.7 \u00b5g\/mL), when compared to synthetic antioxidants. Chlorogenic acids and several flavonoids were identified and quantified by UPLC-DAD-MSn. The di-O-caffeoylquinic acids isomers were the most concentrated phenolics (25.4 mg\/g DW) in the methanolic extract.<\/jats:p>","DOI":"10.3390\/metabo11050280","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T22:29:07Z","timestamp":1619648947000},"page":"280","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Leafy Stems of Phagnalon saxatile subsp. saxatile from Algeriaas a Source of Chlorogenic Acids and Flavonoids with Antioxidant Activity: Characterization and Quantification Using UPLC-DAD-ESI-MSn"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1204-3337","authenticated-orcid":false,"given":"Farah","family":"Haddouchi","sequence":"first","affiliation":[{"name":"Natural Products Laboratory, Department of Biology, Abou Bekr Belkaid University, B.P 119, Tlemcen 13000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarik Mohammed","family":"Chaouche","sequence":"additional","affiliation":[{"name":"Natural Products Laboratory, Department of Biology, Abou Bekr Belkaid University, B.P 119, Tlemcen 13000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riadh","family":"Ksouri","sequence":"additional","affiliation":[{"name":"Laboratory of Aromatic and Medicinal Plants, Biotechnology Center of Borj-C\u00e9dria, B.P 901, Hammam-Lif 2050, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9060-5040","authenticated-orcid":false,"given":"Romain","family":"Larbat","sequence":"additional","affiliation":[{"name":"Laboratoire Agronomie et Environnement, Universit\u00e9 de Lorraine, INRAE, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,29]]},"reference":[{"key":"ref_1","first-page":"473","article-title":"Cypsela morphology of some genera in the tribe Gnaphalieae (Asteraceae) from Pakistan","volume":"40","author":"Abid","year":"2008","journal-title":"Pak. 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