{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T09:37:41Z","timestamp":1792143461037,"version":"4.4.0"},"reference-count":44,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,8,25]],"date-time":"2021-08-25T00:00:00Z","timestamp":1629849600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Vismione H (VH) is a fluorescent prenylated anthranoid produced by plants from the Hypericaceae family, with antiprotozoal activities against malaria and leishmaniosis. Little is known about its biosynthesis and metabolism in plants or its mode of action against parasites. When VH is isolated from Psorospermum glaberrimum, it is rapidly converted into madagascine anthrone and anthraquinone, which are characterized by markedly different fluorescent properties. To locate the fluorescence of VH in living plant cells and discriminate it from that of the other metabolites, an original strategy combining spectral imaging (SImaging), confocal microscopy, and non-targeted metabolomics using mass spectrometry, was developed. Besides VH, structurally related molecules including madagascine (Mad), emodin (Emo), quinizarin (Qui), as well as lapachol (Lap) and fraxetin (Fra) were analyzed. This strategy readily allowed a spatiotemporal characterization and discrimination of spectral fingerprints from anthranoid-derived metabolites and related complexes with cations and proteins. In addition, our study validates the ability of plant cells to metabolize VH into madagascine anthrone, anthraquinones and unexpected metabolites. These results pave the way for new hypotheses on anthranoid metabolism in plants.<\/jats:p>","DOI":"10.3390\/metabo11090571","type":"journal-article","created":{"date-parts":[[2021,8,25]],"date-time":"2021-08-25T23:25:50Z","timestamp":1629933950000},"page":"571","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Unravelling the Puzzle of Anthranoid Metabolism in Living Plant Cells Using Spectral Imaging Coupled to Mass Spectrometry"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9231-7459","authenticated-orcid":false,"given":"Quentin","family":"Chevalier","sequence":"first","affiliation":[{"name":"Centre National de la Recherche Scientifique, Laboratoire d\u2019Innovation Th\u00e9rapeutique, Universit\u00e9 de Strasbourg, CEDEX, F-67401 Illkirch, France"},{"name":"Institut de Biologie Mol\u00e9culaire des Plantes, Centre National de la Recherche Scientifique, Universit\u00e9 de Strasbourg, CEDEX, F-67084 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Baptiste","family":"Gall\u00e9","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique, Laboratoire d\u2019Innovation Th\u00e9rapeutique, Universit\u00e9 de Strasbourg, CEDEX, F-67401 Illkirch, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Wasser","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique, Laboratoire d\u2019Innovation Th\u00e9rapeutique, Universit\u00e9 de Strasbourg, CEDEX, F-67401 Illkirch, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9230-4404","authenticated-orcid":false,"given":"Val\u00e9rie","family":"Mazan","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique, Laboratoire d\u2019Innovation Mol\u00e9culaire et Applications, Universit\u00e9 de Strasbourg-Universit\u00e9 de Haute Alsace, CEDEX, F-67087 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2564-5529","authenticated-orcid":false,"given":"Claire","family":"Villette","sequence":"additional","affiliation":[{"name":"Institut de Biologie Mol\u00e9culaire des Plantes, Centre National de la Recherche Scientifique, Universit\u00e9 de Strasbourg, CEDEX, F-67084 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Mutterer","sequence":"additional","affiliation":[{"name":"Institut de Biologie Mol\u00e9culaire des Plantes, Centre National de la Recherche Scientifique, Universit\u00e9 de Strasbourg, CEDEX, F-67084 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Mercedes","family":"Elustondo","sequence":"additional","affiliation":[{"name":"SUPINFO \u00c9cole des Experts des M\u00e9tiers de l\u2019Informatique, CEDEX, F-67004 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4610-9872","authenticated-orcid":false,"given":"Nicolas","family":"Girard","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique, Laboratoire d\u2019Innovation Th\u00e9rapeutique, Universit\u00e9 de Strasbourg, CEDEX, F-67401 Illkirch, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mourad","family":"Elhabiri","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique, Laboratoire d\u2019Innovation Mol\u00e9culaire et Applications, Universit\u00e9 de Strasbourg-Universit\u00e9 de Haute Alsace, CEDEX, F-67087 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4099-4447","authenticated-orcid":false,"given":"Hubert","family":"Schaller","sequence":"additional","affiliation":[{"name":"Institut de Biologie Mol\u00e9culaire des Plantes, Centre National de la Recherche Scientifique, Universit\u00e9 de Strasbourg, CEDEX, F-67084 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3383-7355","authenticated-orcid":false,"given":"Andr\u00e9a","family":"Hemmerlin","sequence":"additional","affiliation":[{"name":"Institut de Biologie Mol\u00e9culaire des Plantes, Centre National de la Recherche Scientifique, Universit\u00e9 de Strasbourg, CEDEX, F-67084 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Vonthron-S\u00e9n\u00e9cheau","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique, Laboratoire 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