{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:55:57Z","timestamp":1787028957866,"version":"build-2736575974"},"reference-count":68,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,12,3]],"date-time":"2021-12-03T00:00:00Z","timestamp":1638489600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010198","name":"Ministry of Economy, Industry and Competitiveness","doi-asserted-by":"publisher","award":["BFU2016-78951-R"],"award-info":[{"award-number":["BFU2016-78951-R"]}],"id":[{"id":"10.13039\/501100010198","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010198","name":"Ministry of Economy, Industry and Competitiveness","doi-asserted-by":"publisher","award":["BFU2017-90578-REDT"],"award-info":[{"award-number":["BFU2017-90578-REDT"]}],"id":[{"id":"10.13039\/501100010198","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012818","name":"Comunidad de Madrid","doi-asserted-by":"publisher","award":["B2017\/BMD-3684"],"award-info":[{"award-number":["B2017\/BMD-3684"]}],"id":[{"id":"10.13039\/100012818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PID2020-116875RB-I00"],"award-info":[{"award-number":["PID2020-116875RB-I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["MICINN RTI2018-095166-B-I00"],"award-info":[{"award-number":["MICINN RTI2018-095166-B-I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007885","name":"European Association for the Study of Diabetes","doi-asserted-by":"publisher","award":["Albert Renold Travel Fellowship Program"],"award-info":[{"award-number":["Albert Renold Travel Fellowship Program"]}],"id":[{"id":"10.13039\/501100007885","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Severe obesity is a major risk for chronic kidney disease (CKD). Early detection and careful monitoring of renal function are critical for the prevention of CKD during obesity, since biopsies are not performed in patients with CKD and diagnosis is dependent on the assessment of clinical parameters. To explore whether distinct lipid and metabolic signatures in obesity may signify early stages of pathogenesis toward CKD, liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-high resolution accurate mass-mass spectrometry (GC-HRAM-MS) analyses were performed in the serum and the urine of severely obese patients with and without CKD. Moreover, the impact of bariatric surgery (BS) in lipid and metabolic signature was also studied, through LC-MS and GC-HRAM-MS analyses in the serum and urine of patients with severe obesity and CKD before and after undergoing BS. Regarding patients with severe obesity and CKD compared to severely obese patients without CKD, serum lipidome analysis revealed significant differences in lipid signature. Furthermore, serum metabolomics profile revealed significant changes in specific amino acids, with isoleucine and tyrosine, increased in CKD patients compared with patients without CKD. LC-MS and GC-HRAM-MS analysis in serum of patients with severe obesity and CKD after BS showed downregulation of levels of triglycerides (TGs) and diglycerides (DGs) as well as a decrease in branched-chain amino acid (BCAA), lysine, threonine, proline, and serine. In addition, BS removed most of the correlations in CKD patients against biochemical parameters related to kidney dysfunction. Concerning urine analysis, hippuric acid, valine and glutamine were significantly decreased in urine from CKD patients after surgery. Interestingly, bariatric surgery did not restore all the lipid species, some of them decreased, hence drawing attention to them as potential targets for early diagnosis or therapeutic intervention. Results obtained in this study would justify the use of comprehensive mass spectrometry-based lipidomics to measure other lipids aside from conventional lipid profiles and to validate possible early markers of risk of CKD in patients with severe obesity.<\/jats:p>","DOI":"10.3390\/metabo11120836","type":"journal-article","created":{"date-parts":[[2021,12,5]],"date-time":"2021-12-05T21:01:45Z","timestamp":1638738105000},"page":"836","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Lipidomic and Metabolomic Signature of Progression of Chronic Kidney Disease in Patients with Severe Obesity"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9907-4496","authenticated-orcid":false,"given":"Borja","family":"Lanzon","sequence":"first","affiliation":[{"name":"LIPOBETA Group, Department Basic Sciences of Health, Faculty of Sciences of Health, Universidad Rey Juan Carlos, 28922 Alcorc\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9057-4494","authenticated-orcid":false,"given":"Marina","family":"Martin-Taboada","sequence":"additional","affiliation":[{"name":"LIPOBETA Group, Department Basic Sciences of Health, Faculty of Sciences of Health, Universidad Rey Juan Carlos, 28922 Alcorc\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9535-6821","authenticated-orcid":false,"given":"Victor","family":"Castro-Alves","sequence":"additional","affiliation":[{"name":"School of Science and Technology, \u00d6rebro University, 702 81 \u00d6rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0973-5156","authenticated-orcid":false,"given":"Rocio","family":"Vila-Bedmar","sequence":"additional","affiliation":[{"name":"LIPOBETA Group, Department Basic Sciences of Health, Faculty of Sciences of Health, Universidad Rey Juan Carlos, 28922 Alcorc\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Gonz\u00e1lez de Pablos","sequence":"additional","affiliation":[{"name":"Department of Nephrology, University Hospital 12 de Octubre, 28041 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Duberg","sequence":"additional","affiliation":[{"name":"School of Science and Technology, \u00d6rebro University, 702 81 \u00d6rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilar","family":"Gomez","sequence":"additional","affiliation":[{"name":"Department of Surgery, Hospital Universitario 12 de Octubre, 28041 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elias","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Department of Surgery, Hospital Universitario 12 de Octubre, 28041 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2856-9165","authenticated-orcid":false,"given":"Matej","family":"Ore\u0161i\u010d","sequence":"additional","affiliation":[{"name":"School of Medical Sciences, \u00d6rebro University, 702 81 \u00d6rebro, Sweden"},{"name":"Turku Bioscience, University of Turku and \u00c5bo Akademi University, 20520 Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1389-8302","authenticated-orcid":false,"given":"Tuulia","family":"Hy\u00f6tyl\u00e4inen","sequence":"additional","affiliation":[{"name":"School of Science and Technology, \u00d6rebro University, 702 81 \u00d6rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrique","family":"Morales","sequence":"additional","affiliation":[{"name":"Department of Nephrology, University Hospital 12 de Octubre, 28041 Madrid, Spain"},{"name":"Research Institute of University 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