{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T16:38:09Z","timestamp":1782232689292,"version":"3.54.5"},"reference-count":61,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T00:00:00Z","timestamp":1644278400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["JP18K07623"],"award-info":[{"award-number":["JP18K07623"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>The thrombopoietin receptor agonist romiplostim (RP) was recently approved by the US Food and Drug Administration for improving survival in patients acutely exposed to myelosuppressive doses of radiation. Our previous studies with mice have shown that RP administration after lethal irradiation not only completely rescues irradiated mice but also shows mitigative effects on their hematopoiesis and multiple organ injury, including that of the lung, bone marrow, small intestine, and liver. However, the mechanism by which RP functions as a radiomitigator remains unclear. In the present study, we applied a metabolomics approach, which has the ability to reflect the status of an organism directly and accurately, helping to elucidate the biology of treatment responses. Our results showed that the disruption of several metabolites and pathways in response to total body irradiation was partially corrected by RP administration. Notably, RP-corrected metabolites and pathways have been reported to be indicators of DNA damage and lung, bone marrow, small intestine, and liver injury. Taken together, the present findings suggested that the radiomitigative effect of RP is partially involved in the recovery of organ injury, and the identified metabolites may be a useful biomarker of the survival likelihood following radiation exposure.<\/jats:p>","DOI":"10.3390\/metabo12020161","type":"journal-article","created":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T23:37:48Z","timestamp":1644363468000},"page":"161","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["An Analysis of the Serum Metabolomic Profile for the Radiomitigative Effect of the Thrombopoietin Receptor Agonist Romiplostim in Lethally Whole-Body-Irradiated Mice"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3375-5189","authenticated-orcid":false,"given":"Yoshiaki","family":"Sato","sequence":"first","affiliation":[{"name":"Department of Radiation Science, Hirosaki University Graduate School of Health Sciences, Hirosaki 036-8564, Aomori, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaru","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Radiation Science, Hirosaki University Graduate School of Health Sciences, Hirosaki 036-8564, Aomori, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7399-1445","authenticated-orcid":false,"given":"Ikuo","family":"Kashiwakura","sequence":"additional","affiliation":[{"name":"Department of Radiation Science, Hirosaki University Graduate School of Health Sciences, Hirosaki 036-8564, Aomori, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1097\/HP.0000000000000350","article-title":"The Effect of Radiation Dose and Variation in Neupogen\u00ae Initiation Schedule on the Mitigation of Myelosuppression during the Concomitant GI-ARS and H-ARS in a Nonhuman Primate Model of High-Dose Exposure with Marrow Sparing","volume":"109","author":"MacVittie","year":"2015","journal-title":"Health Phys."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S32","DOI":"10.1001\/dmp.2011.17","article-title":"Radiation Injury after a Nuclear Detonation: Medical Consequences and the Need for Scarce Resources Allocation","volume":"5","author":"DiCarlo","year":"2011","journal-title":"Disaster Med. Public Health Prep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1080\/09553002.2017.1338782","article-title":"A Review of Radiation Countermeasures Focusing on Injury-Specific Medicinals and Regulatory Approval Status: Part II. Countermeasures for Limited Indications, Internalized Radionuclides, Emesis, Late Effects, and Agents Demonstrating Efficacy in Large Animals with or without FDA IND Status","volume":"93","author":"Singh","year":"2017","journal-title":"Int. J Radiat. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1080\/09553002.2017.1332440","article-title":"A Review of Radiation Countermeasures Focusing on Injury-Specific Medicinals and Regulatory Approval Status: Part III. Countermeasures under Early Stages of Development along with \u201cstandard of Care\u201d Medicinal and Procedures Not Requiring Regulatory Approval for Use","volume":"93","author":"Singh","year":"2017","journal-title":"Int. J. Radiat. Biol."},{"key":"ref_5","first-page":"1","article-title":"Radioprotective\/Mitigative Effects of Thrombopoietin Receptor Agonists","volume":"10","author":"Kashiwakura","year":"2021","journal-title":"Radiat. Environ. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2034","DOI":"10.1056\/NEJMra052706","article-title":"Lineage-Specific Hematopoietic Growth Factors","volume":"354","author":"Kaushansky","year":"2006","journal-title":"N. Engl. J. Med."},{"key":"ref_7","first-page":"216","article-title":"Thrombopoietin: Biology and Clinical Potentials","volume":"70","author":"Miyazaki","year":"1999","journal-title":"Int. J. Hematol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/S0037-1963(00)90050-7","article-title":"Clinical Experience with Recombinant Human Thrombopoietin in Chemotherapy-Induced Thrombocytopenia","volume":"37","year":"2000","journal-title":"Semin. Hematol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1111\/bjh.12772","article-title":"Thrombopoietin from Beginning to End","volume":"165","author":"Hitchcock","year":"2014","journal-title":"Br. J. Haematol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.beha.2014.10.012","article-title":"The New Thrombopoietic Agenda: Impact on Leukemias and MDS","volume":"27","author":"Bussel","year":"2014","journal-title":"Best Pract. Res. Clin. Haematol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.3324\/haematol.2016.161968","article-title":"Safety and Efficacy of Romiplostim in Splenectomized and Nonsplenectomized Patients with Primary Immune Thrombocytopenia","volume":"102","author":"Cines","year":"2017","journal-title":"Haematologica"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1080\/14656566.2020.1841748","article-title":"An Evaluation of Avatrombopag for the Treatment of Thrombocytopenia","volume":"22","author":"Virk","year":"2021","journal-title":"Expert Opin. Pharmacother."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.freeradbiomed.2019.03.023","article-title":"Diverse Functions of the Thrombopoietin Receptor Agonist Romiplostim Rescue Individuals Exposed to Lethal Radiation","volume":"136","author":"Yamaguchi","year":"2019","journal-title":"Free. Radic. Biol. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1876","DOI":"10.1248\/bpb.b20-00442","article-title":"Regulation of Antioxidant Stress-Responsive Transcription Factor Nrf2 Target Gene in the Reduction of Radiation Damage by the Thrombocytopenia Drug Romiplostim","volume":"43","author":"Chiba","year":"2020","journal-title":"Biol. Pharm. Bull."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1038\/nrd.2016.32","article-title":"Emerging Applications of Metabolomics in Drug Discovery and Precision Medicine","volume":"15","author":"Wishart","year":"2016","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1038\/ijo.2015.65","article-title":"The Metabolome Profiling and Pathway Analysis in Metabolic Healthy and Abnormal Obesity","volume":"39","author":"Chen","year":"2015","journal-title":"Int. J. Obes."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"84","DOI":"10.3389\/fmolb.2017.00084","article-title":"Combined Metabolomic Analysis of Plasma and Urine Reveals AHBA, Tryptophan and Serotonin Metabolism as Potential Risk Factors in Gestational Diabetes Mellitus (GDM)","volume":"4","author":"Leitner","year":"2017","journal-title":"Front. Mol. Biosci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"59189","DOI":"10.18632\/oncotarget.10830","article-title":"Prediction and Diagnosis of Renal Cell Carcinoma Using Nuclear Magnetic Resonance-Based Serum Metabolomics and Self-Organizing Maps","volume":"7","author":"Zheng","year":"2016","journal-title":"Oncotarget"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1080\/09553002.2016.1269218","article-title":"Metabolomic Applications in Radiation Biodosimetry: Exploring Radiation Effects through Small Molecules","volume":"93","author":"Pannkuk","year":"2017","journal-title":"Int. J. Radiat. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1080\/09553002.2017.1304590","article-title":"Systemic Effects of Ionizing Radiation at the Proteome and Metabolome Levels in the Blood of Cancer Patients Treated with Radiotherapy: The Influence of Inflammation and Radiation Toxicity","volume":"93","author":"Jelonek","year":"2017","journal-title":"Int. J. Radiat. Biol."},{"key":"ref_21","first-page":"190","article-title":"Mitigative Effects of a Combination of Multiple Pharmaceutical Drugs on the Survival of Mice Exposed to Lethal Ionizing Radiation","volume":"17","author":"Tokuhisa","year":"2016","journal-title":"Curr. Pharm. Biotechnol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"10659","DOI":"10.1038\/s41598-018-29013-5","article-title":"The Thrombopoietin Mimetic Romiplostim Leads to the Complete Rescue of Mice Exposed to Lethal Ionizing Radiation","volume":"8","author":"Yamaguchi","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1592\/phco.30.7.666","article-title":"Treatment Options for Chronic Refractory Idiopathic Thrombocytopenic Purpura in Adults: Focus on Romiplostim and Eltrombopag","volume":"30","author":"Chouhan","year":"2010","journal-title":"Pharmacother. J. Hum. Pharmacol. Drug Ther."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/978-1-61737-985-7_17","article-title":"Processing and Analysis of GC\/LC-MS-Based Metabolomics Data","volume":"708","author":"Want","year":"2011","journal-title":"Methods Mol. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Pannkuk, E.L., Laiakis, E.C., Girgis, M., Dowd, S.E., Dhungana, S., Nishita, D., Bujold, K., Bakke, J., Gahagen, J., and Authier, S. (2019). Temporal Effects on Radiation Responses in Nonhuman Primates: Identification of Biofluid Small Molecule Signatures by Gas Chromatography\u2013Mass Spectrometry Metabolomics. Metabolites, 9.","DOI":"10.1101\/620526"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.ijrobp.2014.10.023","article-title":"Intestinal Microbiota-Derived Metabolomic Blood Plasma Markers for Prior Radiation Injury","volume":"91","author":"Broin","year":"2015","journal-title":"Int. J. Radiat. Oncol. Biol. Phys."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Kurland, I.J., Broin, P.\u00d3., Golden, A., Su, G., Meng, F., Liu, L., Mohney, R., Kulkarni, S., and Guha, C. (2015). Integrative Metabolic Signatures for Hepatic Radiation Injury. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0124795"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Cheema, A.K., Suman, S., Kaur, P., Singh, R., Fornace, A.J., and Datta, K. (2014). Long-Term Differential Changes in Mouse Intestinal Metabolomics after \u03b3 and Heavy Ion Radiation Exposure. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0087079"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"eaay9097","DOI":"10.1126\/science.aay9097","article-title":"Multi-Omics Analyses of Radiation Survivors Identify Radioprotective Microbes and Metabolites","volume":"370","author":"Guo","year":"2020","journal-title":"Science"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"283","DOI":"10.3109\/09553000903564067","article-title":"Histamine Protects Bone Marrow against Cellular Damage Induced by Ionising Radiation","volume":"86","author":"Medina","year":"2010","journal-title":"Int. J. Radiat. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1080\/09553005914550051","article-title":"5-Hydroxytryptamine as a Radiation Protective Substance in Animals","volume":"1","author":"Langendorff","year":"1959","journal-title":"Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/jrr\/rru087","article-title":"Comparative Efficacy and the Window of Radioprotection for Adrenergic and Serotoninergic Agents and Aminothiols in Experiments with Small and Large Animals","volume":"56","author":"Vasin","year":"2015","journal-title":"J. Radiat. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1667\/RR1703.1","article-title":"Radiation Metabolomics. 2. Dose- and Time-Dependent Urinary Excretion of Deaminated Purines and Pyrimidines after Sublethal Gamma-Radiation Exposure in Mice","volume":"172","author":"Tyburski","year":"2009","journal-title":"Radiat. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1080\/09553000701570238","article-title":"Mechanisms Underlying the Radioprotective Effect of Histamine on Small Intestine","volume":"83","author":"Medina","year":"2007","journal-title":"Int. J. Radiat. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e48","DOI":"10.4081\/ejh.2012.e48","article-title":"Radioprotective Potential of Histamine on Rat Small Intestine and Uterus","volume":"56","author":"Carabajal","year":"2012","journal-title":"Eur. J. Histochem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"11018","DOI":"10.1111\/jcmm.15662","article-title":"Glucosamine Protects against Radiation-Induced Lung Injury via Inhibition of Epithelial-Mesenchymal Transition","volume":"24","author":"Lei","year":"2020","journal-title":"J. Cell. Mol. Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"189","DOI":"10.3181\/00379727-84-20586","article-title":"Comparative Protective Effect of Cysteine against Fat Neutron and Gamma Irradiation in Mice","volume":"84","author":"Patt","year":"1953","journal-title":"Proc. Soc. Exp. Biol. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1461","DOI":"10.1038\/sj.onc.1201310","article-title":"Resistance to Radiation-Induced Apoptosis in Bcl-2-Expressing Cells Is Reversed by Depleting Cellular Thiols","volume":"15","author":"Mirkovic","year":"1997","journal-title":"Oncogene"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1667\/RR2990.1","article-title":"Low-Dose Ionizing Radiation-Induced Blood Plasma Metabolic Response in a Diverse Genetic Mouse Population","volume":"178","author":"Lee","year":"2012","journal-title":"Radiat. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1182\/blood-2016-10-740241","article-title":"Eltrombopag: A Powerful Chelator of Cellular or Extracellular Iron (III) Alone or Combined with a Second Chelator","volume":"130","author":"Vlachodimitropoulou","year":"2017","journal-title":"Blood"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"25","DOI":"10.2174\/157339913804143225","article-title":"Regulation of Insulin Synthesis and Secretion and Pancreatic Beta-Cell Dysfunction in Diabetes","volume":"9","author":"Fu","year":"2013","journal-title":"Curr. Diabetes Rev."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1016\/j.cmet.2014.10.008","article-title":"DNA Damage Response and Metabolic Disease","volume":"20","author":"Shimizu","year":"2014","journal-title":"Cell Metab."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3573","DOI":"10.2337\/db16-0364","article-title":"Ionizing Radiation Potentiates High-Fat Diet-Induced Insulin Resistance and Reprograms Skeletal Muscle and Adipose Progenitor Cells","volume":"65","author":"Nylander","year":"2016","journal-title":"Diabetes"},{"key":"ref_44","first-page":"201","article-title":"Glutamine Utilization by the Small Intestine","volume":"53","author":"Windmueller","year":"1982","journal-title":"Adv. Enzymol. Relat. Areas Mol. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.3109\/0284186X.2011.584557","article-title":"Plasma Citrulline Levels Predict Intestinal Toxicity in Patients Treated with Pelvic Radiotherapy","volume":"50","author":"Onal","year":"2011","journal-title":"Acta Oncol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.ijrobp.2004.02.052","article-title":"Plasma Citrulline Concentration: A Surrogate End Point for Radiation-Induced Mucosal Atrophy of the Small Bowel. A Feasibility Study in 23 Patients","volume":"60","author":"Lutgens","year":"2004","journal-title":"Int. J. Radiat. Oncol. Biol. Phys."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1097\/HP.0000000000000346","article-title":"Citrulline as a Biomarker in the Murine Total-Body Irradiation Model: Correlation of Circulating and Tissue Citrulline to Small Intestine Epithelial Histopathology","volume":"109","author":"Jones","year":"2015","journal-title":"Health Phys."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1097\/HP.0000000000000347","article-title":"Citrulline as a Biomarker in the Non-Human Primate Total- and Partial-Body Irradiation Models: Correlation of Circulating Citrulline to Acute and Prolonged Gastrointestinal Injury","volume":"109","author":"Jones","year":"2015","journal-title":"Health Phys."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1093\/annonc\/mdp596","article-title":"Citrulline-Based Assessment Score: First Choice for Measuring and Monitoring Intestinal Failure after High-Dose Chemotherapy","volume":"21","author":"Herbers","year":"2010","journal-title":"Ann. Oncol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1002\/cncr.20733","article-title":"Monitoring Myeloablative Therapy-Induced Small Bowel Toxicity by Serum Citrulline Concentration: A Comparison with Sugar Permeability Tests","volume":"103","author":"Lutgens","year":"2005","journal-title":"Cancer"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1093\/annonc\/mdn579","article-title":"Loss of Enterocyte Mass Is Accompanied by Diminished Turnover of Enterocytes after Myeloablative Therapy in Haematopoietic Stem-Cell Transplant Recipients","volume":"20","author":"Derikx","year":"2009","journal-title":"Ann. Oncol."},{"key":"ref_52","first-page":"552","article-title":"Alterations of Methionine Metabolism in Hepatocarcinogenesis: The Emergent Role of Glycine N-Methyltransferase in Liver Injury","volume":"31","author":"Simile","year":"2018","journal-title":"Ann. Gastroenterol."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"LoBianco, F.V., Krager, K.J., Carter, G.S., Alam, S., Yuan, Y., Lavoie, E.G., Dranoff, J.A., and Aykin-Burns, N. (2020). The Role of Sirtuin 3 in Radiation-Induced Long-Term Persistent Liver Injury. Antioxidants, 9.","DOI":"10.3390\/antiox9050409"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1080\/09553002.2020.1787546","article-title":"Proteomic Changes by Radio-Mitigative Thrombopoietin Receptor Agonist Romiplostim in the Blood of Mice Exposed to Lethal Total-Body Irradiation","volume":"96","author":"Nishida","year":"2020","journal-title":"Int. J. Radiat. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1038\/369565a0","article-title":"Cloning and Expression of Murine Thrombopoietin CDNA and Stimulation of Platelet Production In Vivo","volume":"369","author":"Lok","year":"1994","journal-title":"Nature"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1139\/y02-090","article-title":"Thrombopoietin Protects Mice from Mortality and Myelosuppression Following High-Dose Irradiation: Importance of Time Scheduling","volume":"80","author":"Mouthon","year":"2002","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1038\/nature15530","article-title":"A Perisinusoidal Niche for Extramedullary Haematopoiesis in the Spleen","volume":"527","author":"Inra","year":"2015","journal-title":"Nature"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1039\/B714176A","article-title":"Depiction of Metabolome Changes in Histidine-Starved Escherichia Coli by CE-TOFMS","volume":"4","author":"Ohashi","year":"2008","journal-title":"Mol. Biosyst."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1039\/c0mb00141d","article-title":"Metabolomic Anatomy of an Animal Model Revealing Homeostatic Imbalances in Dyslipidaemia","volume":"7","author":"Ooga","year":"2011","journal-title":"Mol. Biosyst."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1007\/s11306-009-0178-y","article-title":"Capillary Electrophoresis Mass Spectrometry-Based Saliva Metabolomics Identified Oral, Breast and Pancreatic Cancer-Specific Profiles","volume":"6","author":"Sugimoto","year":"2010","journal-title":"Metabolomics"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"W388","DOI":"10.1093\/nar\/gkab382","article-title":"MetaboAnalyst 5.0: Narrowing the Gap between Raw Spectra and Functional Insights","volume":"49","author":"Pang","year":"2021","journal-title":"Nucleic Acids Res."}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/12\/2\/161\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:16:29Z","timestamp":1760134589000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/12\/2\/161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,8]]},"references-count":61,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["metabo12020161"],"URL":"https:\/\/doi.org\/10.3390\/metabo12020161","relation":{},"ISSN":["2218-1989"],"issn-type":[{"value":"2218-1989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,8]]}}}