{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:01:38Z","timestamp":1790467298743,"version":"4.1.0"},"reference-count":58,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T00:00:00Z","timestamp":1655424000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Idex Sorbonne University Investments for the Future programs","award":["SU-19-3-EMRG-12"],"award-info":[{"award-number":["SU-19-3-EMRG-12"]}]},{"name":"Idex Sorbonne University Investments for the Future programs","award":["R01 DE14036"],"award-info":[{"award-number":["R01 DE14036"]}]},{"name":"National Institutes of Health","award":["SU-19-3-EMRG-12"],"award-info":[{"award-number":["SU-19-3-EMRG-12"]}]},{"name":"National Institutes of Health","award":["R01 DE14036"],"award-info":[{"award-number":["R01 DE14036"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>The transcription factor Kr\u00fcppel-like factor 10 (Klf10), also known as Tieg1 for TGF\u03b2 (Inducible Early Gene-1) is known to control numerous genes in many cell types that are involved in various key biological processes (differentiation, proliferation, apoptosis, inflammation), including cell metabolism and human disease. In skeletal muscle, particularly in the soleus, deletion of the Klf10 gene (Klf10 KO) resulted in ultrastructure fiber disorganization and mitochondrial metabolism deficiencies, characterized by muscular hypertrophy. To determine the metabolic profile related to loss of Klf10 expression, we analyzed blood and soleus tissue using UHPLC-Mass Spectrometry. Metabolomics analyses on both serum and soleus revealed profound differences between wild-type (WT) and KO animals. Klf10 deficient mice exhibited alterations in metabolites associated with energetic metabolism. Additionally, chemical classes of aromatic and amino-acid compounds were disrupted, together with Krebs cycle intermediates, lipids and phospholipids. From variable importance in projection (VIP) analyses, the Warburg effect, citric acid cycle, gluconeogenesis and transfer of acetyl groups into mitochondria appeared to be possible pathways involved in the metabolic alterations observed in Klf10 KO mice. These studies have revealed essential roles for Klf10 in regulating multiple metabolic pathways whose alterations may underlie the observed skeletal muscle defects as well as other diseases.<\/jats:p>","DOI":"10.3390\/metabo12060556","type":"journal-article","created":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T11:45:44Z","timestamp":1655466344000},"page":"556","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Serum and Soleus Metabolomics Signature of Klf10 Knockout Mice to Identify Potential Biomarkers"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2974-791X","authenticated-orcid":false,"given":"Nadine","family":"Baroukh","sequence":"first","affiliation":[{"name":"UMR 1253, iBrain, University of Tours, Inserm, 37044 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan","family":"Canteleux","sequence":"additional","affiliation":[{"name":"UMR 1253, iBrain, University of Tours, Inserm, 37044 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Lef\u00e8vre","sequence":"additional","affiliation":[{"name":"UMR 1253, iBrain, University of Tours, Inserm, 37044 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0063-4380","authenticated-orcid":false,"given":"Camille","family":"Dupuy","sequence":"additional","affiliation":[{"name":"UMR 1253, iBrain, University of Tours, Inserm, 37044 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Martias","sequence":"additional","affiliation":[{"name":"UMR 1253, iBrain, University of Tours, Inserm, 37044 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3483-7063","authenticated-orcid":false,"given":"Antoine","family":"Presset","sequence":"additional","affiliation":[{"name":"UMR 1253, iBrain, University of Tours, Inserm, 37044 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malayannan","family":"Subramaniam","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Hawse","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5324-2164","authenticated-orcid":false,"given":"Patrick","family":"Emond","sequence":"additional","affiliation":[{"name":"UMR 1253, iBrain, University of Tours, Inserm, 37044 Tours, France"},{"name":"CHRU Tours, Medical Biology Center, 37000 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9700-040X","authenticated-orcid":false,"given":"Philippe","family":"Pouletaut","sequence":"additional","affiliation":[{"name":"Biomechanics and Bioengineering Laboratory, CNRS UMR 7338, Universit\u00e9 de Technologie de Compi\u00e8gne, 60200 Compi\u00e8gne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Morandat","sequence":"additional","affiliation":[{"name":"Biomechanics and Bioengineering Laboratory, CNRS UMR 7338, Universit\u00e9 de Technologie de Compi\u00e8gne, 60200 Compi\u00e8gne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine F.","family":"Bensamoun","sequence":"additional","affiliation":[{"name":"Biomechanics and Bioengineering Laboratory, CNRS UMR 7338, Universit\u00e9 de Technologie de Compi\u00e8gne, 60200 Compi\u00e8gne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydie","family":"Nadal-Desbarats","sequence":"additional","affiliation":[{"name":"UMR 1253, iBrain, University of Tours, Inserm, 37044 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1002\/(SICI)1097-4644(19980201)68:2<226::AID-JCB9>3.0.CO;2-X","article-title":"Tissue, cell type, and breast cancer stage-specific expression of a TGF-beta inducible early transcription factor gene","volume":"68","author":"Subramaniam","year":"1998","journal-title":"J. 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