{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T14:24:09Z","timestamp":1788877449597,"version":"build-2803163510"},"reference-count":42,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,6,25]],"date-time":"2022-06-25T00:00:00Z","timestamp":1656115200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"ISCIII","doi-asserted-by":"publisher","award":["PI18\/01467"],"award-info":[{"award-number":["PI18\/01467"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"ISCIII","doi-asserted-by":"publisher","award":["PI19\/00044"],"award-info":[{"award-number":["PI19\/00044"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"ISCIII","doi-asserted-by":"publisher","award":["RD16\/0006\/0015"],"award-info":[{"award-number":["RD16\/0006\/0015"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"ISCIII","doi-asserted-by":"publisher","award":["PCI2018-092930"],"award-info":[{"award-number":["PCI2018-092930"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FEDER \u201cInvesting in your future\u201d for the thematic network and co-operative research centers ARADyAL","award":["PI18\/01467"],"award-info":[{"award-number":["PI18\/01467"]}]},{"name":"FEDER \u201cInvesting in your future\u201d for the thematic network and co-operative research centers ARADyAL","award":["PI19\/00044"],"award-info":[{"award-number":["PI19\/00044"]}]},{"name":"FEDER \u201cInvesting in your future\u201d for the thematic network and co-operative research centers ARADyAL","award":["RD16\/0006\/0015"],"award-info":[{"award-number":["RD16\/0006\/0015"]}]},{"name":"FEDER \u201cInvesting in your future\u201d for the thematic network and co-operative research centers ARADyAL","award":["PCI2018-092930"],"award-info":[{"award-number":["PCI2018-092930"]}]},{"name":"Ministry of Science and Innovation in Spain","award":["PI18\/01467"],"award-info":[{"award-number":["PI18\/01467"]}]},{"name":"Ministry of Science and Innovation in Spain","award":["PI19\/00044"],"award-info":[{"award-number":["PI19\/00044"]}]},{"name":"Ministry of Science and Innovation in Spain","award":["RD16\/0006\/0015"],"award-info":[{"award-number":["RD16\/0006\/0015"]}]},{"name":"Ministry of Science and Innovation in Spain","award":["PCI2018-092930"],"award-info":[{"award-number":["PCI2018-092930"]}]},{"name":"European program ERA HDHL\u2014Nutrition and the Epigenome","award":["PI18\/01467"],"award-info":[{"award-number":["PI18\/01467"]}]},{"name":"European program ERA HDHL\u2014Nutrition and the Epigenome","award":["PI19\/00044"],"award-info":[{"award-number":["PI19\/00044"]}]},{"name":"European program ERA HDHL\u2014Nutrition and the Epigenome","award":["RD16\/0006\/0015"],"award-info":[{"award-number":["RD16\/0006\/0015"]}]},{"name":"European program ERA HDHL\u2014Nutrition and the Epigenome","award":["PCI2018-092930"],"award-info":[{"award-number":["PCI2018-092930"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>The transition from mild to severe allergic phenotypes is still poorly understood and there is an urgent need of incorporating new therapies, accompanied by personalized diagnosis approaches. This work presents the development of a novel targeted metabolomic methodology for the analysis of 36 metabolites related to allergic inflammation, including mostly sphingolipids, lysophospholipids, amino acids, and those of energy metabolism previously identified in non-targeted studies. The methodology consisted of two complementary chromatography methods, HILIC and reversed-phase. These were developed using liquid chromatography, coupled to triple quadrupole mass spectrometry (LC-QqQ-MS) in dynamic multiple reaction monitoring (dMRM) acquisition mode and were validated using ICH guidelines. Serum samples from two clinical models of allergic asthma patients were used for method application, which were as follows: (1) corticosteroid-controlled (ICS, n = 6) versus uncontrolled (UC, n = 4) patients, and immunotherapy-controlled (IT, n = 23) versus biologicals-controlled (BIO, n = 12) patients. The results showed significant differences mainly in lysophospholipids using univariate analyses in both models. Multivariate analysis for model 1 was able to distinguish both groups, while for model 2, the results showed the correct classification of all BIO samples within their group. Thus, this methodology can be of great importance for further understanding the role of these metabolites in allergic diseases as potential biomarkers for disease severity and for predicting patient treatment response.<\/jats:p>","DOI":"10.3390\/metabo12070592","type":"journal-article","created":{"date-parts":[[2022,6,26]],"date-time":"2022-06-26T21:01:41Z","timestamp":1656277301000},"page":"592","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Development of a Novel Targeted Metabolomic LC-QqQ-MS Method in Allergic Inflammation"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7875-7327","authenticated-orcid":false,"given":"David","family":"Obeso","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, Facultad de Farmacia, Centre for Metabolomics and Bioanalysis (CEMBIO), Universidad San Pablo CEU, CEU Universities, Urbanizaci\u00f3n Montepr\u00edncipe, 28003 Madrid, Spain"},{"name":"Department of Basic Medical Sciences, Facultad de Medicina, Institute of Applied Molecular Medicine (IMMA), Universidad San Pablo CEU, CEU Universities, Urbanizaci\u00f3n Montepr\u00edncipe, 28003 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4437-6758","authenticated-orcid":false,"given":"Nuria","family":"Contreras","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Facultad de Farmacia, Centre for Metabolomics and Bioanalysis (CEMBIO), Universidad San Pablo CEU, CEU Universities, Urbanizaci\u00f3n Montepr\u00edncipe, 28003 Madrid, Spain"},{"name":"Department of Basic Medical Sciences, Facultad de Medicina, Institute of Applied Molecular Medicine (IMMA), Universidad San Pablo CEU, CEU Universities, Urbanizaci\u00f3n Montepr\u00edncipe, 28003 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Dolores-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Facultad de Farmacia, Centre for Metabolomics and Bioanalysis (CEMBIO), Universidad San Pablo CEU, CEU Universities, Urbanizaci\u00f3n Montepr\u00edncipe, 28003 Madrid, Spain"},{"name":"Laboratory of Pharmaceutical Development Tests, Multidisciplinary Research Unit (UIM), Faculty of Higher Education Cuautitl\u00e1n, National Autonomous University of Mexico, Carr. Cuautitl\u00e1n-Teoloyucan Km 2.5, San Sebasti\u00e1n Xhala, Cuautitl\u00e1n Izcalli 54714, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Carrillo","sequence":"additional","affiliation":[{"name":"Servicio de Alergia, Hospital Universitario de Gran Canaria Doctor Negr\u00edn, 35010 Las Palmas de Gran Canaria, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4722-491X","authenticated-orcid":false,"given":"Coral","family":"Barbas","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Facultad de Farmacia, Centre for Metabolomics and Bioanalysis (CEMBIO), Universidad San Pablo CEU, CEU Universities, Urbanizaci\u00f3n Montepr\u00edncipe, 28003 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5057-5150","authenticated-orcid":false,"given":"Mar\u00eda M.","family":"Escribese","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, Facultad de Medicina, Institute of Applied Molecular Medicine (IMMA), Universidad San Pablo CEU, CEU Universities, Urbanizaci\u00f3n Montepr\u00edncipe, 28003 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6652-2739","authenticated-orcid":false,"given":"Alma","family":"Villase\u00f1or","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Facultad de Farmacia, Centre for Metabolomics and Bioanalysis (CEMBIO), Universidad San Pablo CEU, CEU Universities, Urbanizaci\u00f3n Montepr\u00edncipe, 28003 Madrid, Spain"},{"name":"Department of Basic Medical Sciences, Facultad de Medicina, Institute of Applied Molecular Medicine (IMMA), Universidad San Pablo CEU, CEU Universities, Urbanizaci\u00f3n Montepr\u00edncipe, 28003 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5488-5700","authenticated-orcid":false,"given":"Domingo","family":"Barber","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, Facultad de Medicina, Institute of Applied Molecular Medicine (IMMA), Universidad San Pablo CEU, CEU Universities, Urbanizaci\u00f3n Montepr\u00edncipe, 28003 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.jaci.2013.11.020","article-title":"Food allergy: Epidemiology, pathogenesis, diagnosis, and treatment","volume":"133","author":"Sicherer","year":"2014","journal-title":"J. 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