{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T13:00:38Z","timestamp":1788008438109,"version":"build-2784847793"},"reference-count":13,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,6,26]],"date-time":"2022-06-26T00:00:00Z","timestamp":1656201600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Japan Agency for Medical Research and Development (AMED)","award":["16hm0102042h0001"],"award-info":[{"award-number":["16hm0102042h0001"]}]},{"name":"Japan Agency for Medical Research and Development (AMED)","award":["17hm0102042h0002"],"award-info":[{"award-number":["17hm0102042h0002"]}]},{"name":"Japan Agency for Medical Research and Development (AMED)","award":["18hm0102042h0003"],"award-info":[{"award-number":["18hm0102042h0003"]}]},{"name":"Japan Agency for Medical Research and Development (AMED)","award":["19dk0310099h0001"],"award-info":[{"award-number":["19dk0310099h0001"]}]},{"name":"Japan Agency for Medical Research and Development (AMED)","award":["20dk0310099h0002"],"award-info":[{"award-number":["20dk0310099h0002"]}]},{"name":"Japan Agency for Medical Research and Development (AMED)","award":["21dk0310099h0003"],"award-info":[{"award-number":["21dk0310099h0003"]}]},{"name":"General Insurance Association of Japan","award":["16hm0102042h0001"],"award-info":[{"award-number":["16hm0102042h0001"]}]},{"name":"General Insurance Association of Japan","award":["17hm0102042h0002"],"award-info":[{"award-number":["17hm0102042h0002"]}]},{"name":"General Insurance Association of Japan","award":["18hm0102042h0003"],"award-info":[{"award-number":["18hm0102042h0003"]}]},{"name":"General Insurance Association of Japan","award":["19dk0310099h0001"],"award-info":[{"award-number":["19dk0310099h0001"]}]},{"name":"General Insurance Association of Japan","award":["20dk0310099h0002"],"award-info":[{"award-number":["20dk0310099h0002"]}]},{"name":"General Insurance Association of Japan","award":["21dk0310099h0003"],"award-info":[{"award-number":["21dk0310099h0003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Iron is an essential nutrient in the body. However, iron generates oxidative stress and hence needs to be bound to carrier proteins such as the glycoprotein transferrin (Tf) in body fluids. We previously reported that cerebrospinal fluid contains Tf glycan-isoforms that are derived from the brain, but their origins at the cellular level in the brain have not yet been elucidated. In the present report, we described the localization of Tf protein and mRNA in mouse and human brain tissue. In situ hybridization of mouse brain tissue revealed that Tf mRNA is expressed by different cell types such as epithelial cells in the choroid plexus, oligodendrocyte-like cells in the medulla, and neurons in the cortex, hippocampus, and basal ganglia. In contrast, Tf protein was barely detected by immunohistochemistry in hippocampal and some cortical neurons, but it was detected in other types of cells such as oligodendrocyte-like cells and choroid plexus epithelial cells. The results showed that Tf mRNA is expressed by neural cells, while Tf protein is expressed in different brain regions, though at very low levels in hippocampal neurons. Low Tf level in the hippocampus may increases susceptibility to iron-induced oxidative stress, and account for neuron death in neurodegenerative diseases.<\/jats:p>","DOI":"10.3390\/metabo12070594","type":"journal-article","created":{"date-parts":[[2022,6,26]],"date-time":"2022-06-26T21:01:41Z","timestamp":1656277301000},"page":"594","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Expression of Transferrin Protein and Messenger RNA in Neural Cells from Mouse and Human Brain Tissue"],"prefix":"10.3390","volume":"12","author":[{"given":"Eriko","family":"Abe","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Fukushima Medical University, Fukushima 960-1295, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi J.","family":"Fuwa","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Fukushima Medical University, Fukushima 960-1295, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoka","family":"Hoshi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Fukushima Medical University, Fukushima 960-1295, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Neurocognitive Science, Nagoya City University, Aichi 467-8601, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takenobu","family":"Murakami","sequence":"additional","affiliation":[{"name":"Division of Neurology, Department of Brain and Neurosciences, Faculty of Medicine, Tottori University, Tottori 683-8504, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masakazu","family":"Miyajima","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Juntendo University, Tokyo 113-8421, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norihiro","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, Fukushimura Hospital, Aichi 467-8601, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0089-6033","authenticated-orcid":false,"given":"Hiroyasu","family":"Akatsu","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, Fukushimura Hospital, Aichi 467-8601, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshio","family":"Hashizume","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, Fukushimura Hospital, Aichi 467-8601, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiro","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, Fukushima Medical University, Fukushima 960-1295, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Honda","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, Fukushima Medical University, Fukushima 960-1295, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0009-8981(90)90278-Z","article-title":"The biology of transferrin","volume":"190","year":"1990","journal-title":"Clin. Chim. Acta"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.spen.2006.08.002","article-title":"Brain iron metabolism","volume":"13","author":"Rouault","year":"2006","journal-title":"Semin. Pediatr. 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Aging"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Hoshi, K., Ito, H., Abe, E., Fuwa, T.J., Kanno, M., Murakami, Y., Abe, M., Murakami, T., Yoshihara, A., and Ugawa, Y. (2021). Transferrin biosynthesized in the brain is a novel biomarker for Alzheimer\u2019s disease. Metabolites, 11.","DOI":"10.3390\/metabo11090616"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1093\/jb\/mvy043","article-title":"Rapid increase of \u2018brain-type\u2019 transferrin in cerebrospinal fluid after shunt surgery for idiopathic normal pressure hydrocephalus: A prognosis marker for cognitive recovery","volume":"164","author":"Murakami","year":"2018","journal-title":"J. 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Adv."}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/12\/7\/594\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:38:40Z","timestamp":1760139520000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/12\/7\/594"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,26]]},"references-count":13,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["metabo12070594"],"URL":"https:\/\/doi.org\/10.3390\/metabo12070594","relation":{},"ISSN":["2218-1989"],"issn-type":[{"value":"2218-1989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,26]]}}}