{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T12:30:44Z","timestamp":1786537844199,"version":"build-2736575974"},"reference-count":51,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,7,27]],"date-time":"2022-07-27T00:00:00Z","timestamp":1658880000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences","award":["2020JBFR03"],"award-info":[{"award-number":["2020JBFR03"]}]},{"name":"National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences","award":["2021JBFM13"],"award-info":[{"award-number":["2021JBFM13"]}]},{"name":"National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences","award":["2020JBMF02"],"award-info":[{"award-number":["2020JBMF02"]}]},{"name":"National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences","award":["CX(20)2025"],"award-info":[{"award-number":["CX(20)2025"]}]},{"name":"Jiangsu Agriculture Science and Technology Innovation Fund","award":["2020JBFR03"],"award-info":[{"award-number":["2020JBFR03"]}]},{"name":"Jiangsu Agriculture Science and Technology Innovation Fund","award":["2021JBFM13"],"award-info":[{"award-number":["2021JBFM13"]}]},{"name":"Jiangsu Agriculture Science and Technology Innovation Fund","award":["2020JBMF02"],"award-info":[{"award-number":["2020JBMF02"]}]},{"name":"Jiangsu Agriculture Science and Technology Innovation Fund","award":["CX(20)2025"],"award-info":[{"award-number":["CX(20)2025"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Our previous study demonstrated that low temperature could induce hepatic inflammation and suppress the immune and oxidation resistance of freshwater drum. However, the metabolism, especially the glucose and lipid metabolism involved, is poorly studied. To further explore the chronic hypothermia response of freshwater drum, an 8-day hypothermia experiment was conducted at 10 \u00b0C to investigate the effect of chronic hypothermia on glucose and lipid metabolism via biochemical and physiological indexes, and metabolic enzyme activities, miRNAs and mRNA-miRNA integrate analysis in the liver. Plasma and hepatic biochemical parameters reveal chronic hypothermia-promoted energy expenditure. Metabolic enzyme levels uncover that glycolysis was enhanced but lipid metabolism was suppressed. Differentially expressed miRNAs induced by hypothermia were mainly involved in glucose and lipid metabolism, programmed cell death, disease, and cancerization. Specifically, KEGG enrichment indicates that AMPK signaling was dysregulated. mRNA-miRNA integrated analysis manifests miR-1 and AMPK, which were actively co-related in the regulatory network. Furthermore, transcriptional expression of key genes demonstrates hypothermia-activated AMPK signaling by miR-1 and subsequently inhibited the downstream glucogenic and glycogenic gene expression and gene expression of fatty acid synthesis. However, glycogenesis was alleviated to the control level while fatty acid synthesis was still suppressed at 8 d. Meanwhile, the gene expressions of glycolysis and fatty acid oxidation were augmented under hypothermia. In conclusion, these results suggest that miR-1\/AMPK is an important target for chronic hypothermia control. It provides a theoretical basis for hypothermia resistance on freshwater drum.<\/jats:p>","DOI":"10.3390\/metabo12080697","type":"journal-article","created":{"date-parts":[[2022,7,28]],"date-time":"2022-07-28T03:21:16Z","timestamp":1658978476000},"page":"697","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["miR-1\/AMPK-Mediated Glucose and Lipid Metabolism under Chronic Hypothermia in the Liver of Freshwater Drum, Aplodinotus grunniens"],"prefix":"10.3390","volume":"12","author":[{"given":"Jianxiang","family":"Chen","sequence":"first","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"},{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9424-3331","authenticated-orcid":false,"given":"Changyou","family":"Song","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"},{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibo","family":"Wen","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"},{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangxiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningyuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxia","family":"Li","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"},{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaomiao","family":"Xue","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pao","family":"Xu","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"},{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.fsi.2017.12.046","article-title":"HSP60 and HSP90\u03b2 from blunt snout bream, Megalobrama amblycephala: Molecular cloning, characterization, and comparative response to intermittent thermal stress and Aeromonas hydrophila infection","volume":"74","author":"Song","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"736458","DOI":"10.1016\/j.aquaculture.2021.736458","article-title":"Effects of temperature on growth performance and metabolism of juvenile sea bass (Dicentrarchus labrax)","volume":"537","author":"Zhou","year":"2021","journal-title":"Aquaculture"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Abram, Q.H., Dixon, B., and Katzenback, B.A. (2017). 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