{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T03:27:01Z","timestamp":1783654021861,"version":"3.55.0"},"reference-count":44,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,7,30]],"date-time":"2022-07-30T00:00:00Z","timestamp":1659139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"the European Union\u2019s Horizon 2020 research and innovation programme","doi-asserted-by":"publisher","award":["860303"],"award-info":[{"award-number":["860303"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"the European Union\u2019s Horizon 2020 research and innovation programme","doi-asserted-by":"publisher","award":["RTI2018-094969-B-I00"],"award-info":[{"award-number":["RTI2018-094969-B-I00"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"the Spanish Ministry of Economy and Competitiveness MINECO","doi-asserted-by":"publisher","award":["860303"],"award-info":[{"award-number":["860303"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"the Spanish Ministry of Economy and Competitiveness MINECO","doi-asserted-by":"publisher","award":["RTI2018-094969-B-I00"],"award-info":[{"award-number":["RTI2018-094969-B-I00"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Steroid hormones play a vital role in the regulation of cellular processes, and dysregulation of these metabolites can provoke or aggravate pathological issues, such as autoimmune diseases and cancer. Regulation of steroid hormones involves different organs and biological compartments. Therefore, it is important to accurately determine their levels in tissues and biofluids to monitor changes after challenge or during disease. In this work, we have developed and optimized the extraction and quantification of 11 key members of the different steroid classes, including androgens, estrogens, progestogens and corticoids. The assay consists of a liquid\/liquid extraction step and subsequent quantification by high-resolution liquid chromatography coupled time-of-flight mass spectrometry. The recoveries range between 74.2 to 126.9% and 54.9 to 110.7%, using a cell culture or urine as matrix, respectively. In general, the signal intensity loss due to matrix effect is no more than 30%. The method has been tested in relevant steroidogenic tissues in rat models and it has also been tested in human urine samples. Overall, this assay measures 11 analytes simultaneously in 6 min runtime and it has been applied in adrenal gland, testis, prostate, brain and serum from rats, and urine and extracellular vesicles from humans.<\/jats:p>","DOI":"10.3390\/metabo12080714","type":"journal-article","created":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T02:06:42Z","timestamp":1659319602000},"page":"714","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Simultaneous Quantification of Steroid Hormones Using hrLC-MS in Endocrine Tissues of Male Rats and Human Samples"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6562-9331","authenticated-orcid":false,"given":"Guillermo","family":"Bordanaba-Florit","sequence":"first","affiliation":[{"name":"Exosomes Laboratory, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastiaan van","family":"Liempd","sequence":"additional","affiliation":[{"name":"Metabolomics Platform, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Cabrera","sequence":"additional","affiliation":[{"name":"Metabolomics Platform, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e9lix","family":"Royo","sequence":"additional","affiliation":[{"name":"Exosomes Laboratory, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (Ciberehd), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3133-0670","authenticated-orcid":false,"given":"Juan Manuel","family":"Falc\u00f3n-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Exosomes Laboratory, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Spain"},{"name":"Metabolomics Platform, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (Ciberehd), 28029 Madrid, Spain"},{"name":"Ikerbasque, Basque Foundation for Science, 48011 Bilbao, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1210\/er.2010-0013","article-title":"The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders","volume":"32","author":"Miller","year":"2011","journal-title":"Endocr. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.3389\/fimmu.2018.01332","article-title":"Glucocorticoids, Sex Hormones, and Immunity","volume":"9","author":"Bereshchenko","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.jpba.2017.02.033","article-title":"Simultaneous Quantification of Estrogens, Their Precursors and Conjugated Metabolites in Human Breast Cancer Cells by LC\u2013HRMS without Derivatization","volume":"138","author":"Poschner","year":"2017","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1002\/med.21766","article-title":"Revisiting Prostate Cancer Metabolism: From Metabolites to Disease and Therapy","volume":"41","author":"Cardoso","year":"2021","journal-title":"Med. Res. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1470442","DOI":"10.1080\/20013078.2018.1470442","article-title":"Metabolic Alterations in Urine Extracellular Vesicles Are Associated to Prostate Cancer Pathogenesis and Progression","volume":"7","author":"Torrano","year":"2018","journal-title":"J. Extracell. Vesicles"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"20","DOI":"10.4103\/1477-3163.83937","article-title":"Androgen Receptor Signaling in Prostate Cancer Development and Progression","volume":"10","author":"Lonergan","year":"2011","journal-title":"J. Carcinog."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: Kyoto Encyclopedia of Genes and Genomes","volume":"28","author":"Kanehisa","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"D545","DOI":"10.1093\/nar\/gkaa970","article-title":"KEGG: Integrating Viruses and Cellular Organisms","volume":"49","author":"Kanehisa","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1210\/er.2015-1036","article-title":"The Regulation of Steroid Action by Sulfation and Desulfation","volume":"36","author":"Mueller","year":"2015","journal-title":"Endocr. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"T271","DOI":"10.1530\/JME-18-0086","article-title":"Insights into Steroid Sulfation and Desulfation Pathways","volume":"61","author":"Foster","year":"2018","journal-title":"J. Mol. Endocrinol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.jgg.2016.08.001","article-title":"Genetics and Pathophysiology of Mammalian Sulfate Biology","volume":"44","author":"Langford","year":"2017","journal-title":"J. Genet. Genom."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3163","DOI":"10.1038\/s41596-021-00551-z","article-title":"Using Single-Vesicle Technologies to Unravel the Heterogeneity of Extracellular Vesicles","volume":"16","author":"Royo","year":"2021","journal-title":"Nat. Protoc."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"27066","DOI":"10.3402\/jev.v4.27066","article-title":"Biological Properties of Extracellular Vesicles and Their Physiological Functions","volume":"4","author":"Siljander","year":"2015","journal-title":"J. Extracell. Vesicles"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1371\/journal.pbio.1001450","article-title":"Vesiclepedia: A Compendium for Extracellular Vesicles with Continuous Community Annotation","volume":"10","author":"Kalra","year":"2012","journal-title":"PLoS Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"D516","DOI":"10.1093\/nar\/gky1029","article-title":"Vesiclepedia 2019: A Compendium of RNA, Proteins, Lipids and Metabolites in Extracellular Vesicles","volume":"47","author":"Pathan","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.jchromb.2016.07.024","article-title":"High-Throughput Analysis of 19 Endogenous Androgenic Steroids by Ultra-Performance Convergence Chromatography Tandem Mass Spectrometry","volume":"1031","author":"Quanson","year":"2016","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"121765","DOI":"10.1016\/j.jchromb.2019.121765","article-title":"Simultaneous UHPLC-MS\/MS Method of Estradiol Metabolites to Support the Evaluation of Phase-2 Metabolic Activity of Induced Pluripotent Stem Cell Derived Hepatocytes","volume":"1126\u20131127","author":"Indapurkar","year":"2019","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.jchromb.2019.01.022","article-title":"Simultaneous Determination of Androgens and Prostaglandins in Human Urine Using Ultra-High-Performance Liquid Chromatography\u2013Tandem Mass Spectrometry","volume":"1109","author":"Im","year":"2019","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3404","DOI":"10.1021\/ac702613k","article-title":"Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry Determination of Estrogen Conjugates in Human Urine","volume":"80","author":"Qin","year":"2008","journal-title":"Anal. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Lee, Y.R., Im, E., Kim, H., Lew, B.L., Sim, W.Y., Lee, J., Oh, H.B., Paeng, K.J., Hong, J., and Chung, B.C. (2020). Untargeted Metabolomics and Steroid Signatures in Urine of Male Pattern Baldness Patients after Finasteride Treatment for a Year. Metabolites, 10.","DOI":"10.3390\/metabo10040131"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1093\/jb\/mvs121","article-title":"Accurate Determination of Tissue Steroid Hormones, Precursors and Conjugates in Adult Male Rat","volume":"153","author":"Maeda","year":"2013","journal-title":"J. Biochem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.aquatox.2010.08.003","article-title":"Does Exposure to Testosterone Significantly Alter Endogenous Metabolism in the Marine Mussel Mytilus Galloprovincialis?","volume":"100","author":"Fernandes","year":"2010","journal-title":"Aquat. Toxicol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Li, Y., Yan, Z., Li, X., Yin, X., and Li, K. (2021). Uplc\u2013Tof\u2013Ms Method for Simultaneous Quantification of Steroid Hormones in Tissue Homogenates of Zebrafish with Solid-Phase Extraction. Molecules, 26.","DOI":"10.3390\/molecules26206213"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.cca.2008.11.022","article-title":"Rapid Quantification of Steroid Patterns in Human Serum by On-Line Solid Phase Extraction Combined with Liquid Chromatography-Triple Quadrupole Linear Ion Trap Mass Spectrometry","volume":"401","author":"Ceglarek","year":"2009","journal-title":"Clin. Chim. Acta"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.jsbmb.2011.12.001","article-title":"Standardized LC-MS\/MS Based Steroid Hormone Profile-Analysis","volume":"129","author":"Koal","year":"2012","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1194\/jlr.D119000591","article-title":"An LC\/MS\/MS Method for Analyzing the Steroid Metabolome with High Accuracy and from Small Serum Samples","volume":"61","author":"Yuan","year":"2020","journal-title":"J. Lipid Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"4751","DOI":"10.1007\/s00216-017-0419-2","article-title":"A UHPLC-MS\/MS Method for Profiling Multifunctional Steroids in Human Hair","volume":"409","author":"Dong","year":"2017","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S0021-9673(02)01851-4","article-title":"Determination of Estrogens and Their Conjugates in Water Using Solid-Phase Extraction Followed by Liquid Chromatography-Tandem Mass Spectrometry","volume":"984","author":"Isobe","year":"2003","journal-title":"J. Chromatogr. A"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"7032","DOI":"10.1021\/ac050699x","article-title":"Analysis of Steroid Conjugates in Sewage Influent and Effluent by Liquid Chromatography-Tandem Mass Spectrometry","volume":"77","author":"Reddy","year":"2005","journal-title":"Anal. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"5301","DOI":"10.1007\/s00216-013-6883-4","article-title":"A Fast Method for the Quantitation of Key Metabolites of the Methionine Pathway in Liver Tissue by High-Resolution Mass Spectrometry and Hydrophilic Interaction Ultra-Performance Liquid Chromatography","volume":"405","author":"Cabrera","year":"2013","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S1570-0232(04)00598-7","article-title":"Wide Concentration Range Investigation of Recovery, Precision and Error Structure in Liquid Chromatography","volume":"810","author":"Schepers","year":"2004","journal-title":"J. Chromatogr. B"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Bordanaba-Florit, G. (2022, June 27). Simultaneous Quantification of Steroid Hormones Using hrLC-MS in Endocrine Tissues of Male Rats and Human Samples (Dataset). Available online: https:\/\/figshare.com\/articles\/dataset\/_\/20231493.","DOI":"10.3390\/metabo12080714"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.4155\/bio.11.181","article-title":"Evaluation of Homogenization Techniques for the Preparation of Mouse Tissue Samples to Support Drug Discovery","volume":"3","author":"Liang","year":"2011","journal-title":"Bioanalysis"},{"key":"ref_34","unstructured":"(2022, June 27). Method Validation and Quality Control Procedures for Pesticide Residues Analysis in Food and Feed. Available online: https:\/\/www.eurl-pesticides.eu\/library\/docs\/allcrl\/AqcGuidance_Sanco_2011_12495.pdf."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.jchromb.2012.03.012","article-title":"Influence of Ionization Source Design on Matrix Effects during LC-ESI-MS\/MS Analysis","volume":"893\u2013894","author":"Ghosh","year":"2012","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1839","DOI":"10.4155\/bio-2017-0214","article-title":"Matrix Effects and Application of Matrix Effect Factor","volume":"9","author":"Zhou","year":"2017","journal-title":"Bioanalysis"},{"key":"ref_37","first-page":"18397","article-title":"Diverse Subpopulations of Vesicles Secreted by Different Intracellular Mechanisms Are Present in Exosome Preparations Obtained by Differential Ultracentrifugation","volume":"1","author":"Bobrie","year":"2012","journal-title":"J. Ayurveda Integr. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2401","DOI":"10.1002\/rcm.1641","article-title":"Applications of a Travelling Wave-Based Radio-Frequency-Only Stacked Ring Ion Guide","volume":"18","author":"Giles","year":"2004","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1016\/j.jasms.2010.02.017","article-title":"Scanwave: A New Approach to Enhancing Spectral Data on a Tandem Quadrupole Mass Spectrometer","volume":"21","author":"Kenny","year":"2010","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.clinbiochem.2004.11.007","article-title":"Matrix Effects: The Achilles Heel of Quantitative High-Performance Liquid Chromatography-Electrospray-Tandem Mass Spectrometry","volume":"38","author":"Taylor","year":"2005","journal-title":"Clin. Biochem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.mpaic.2008.07.013","article-title":"Adrenocortical Hormones","volume":"9","author":"Campbell","year":"2008","journal-title":"Anaesth. Intensive Care Med."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1080\/019262301301418847","article-title":"Adrenal Gland: Structure, Function, and Mechanisms of Toxicity","volume":"29","author":"Rosol","year":"2016","journal-title":"Toxicol. Pathol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.jsbmb.2007.09.015","article-title":"Regulation of the Adrenal Androgen Biosynthesis","volume":"108","author":"Rainey","year":"2008","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3824","DOI":"10.7150\/thno.19890","article-title":"Metabolomic Profiling of Extracellular Vesicles and Alternative Normalization Methods Reveal Enriched Metabolites and Strategies to Study Prostate Cancer-Related Changes","volume":"7","author":"Puhka","year":"2017","journal-title":"Theranostics"}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/12\/8\/714\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:00:10Z","timestamp":1760140810000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/12\/8\/714"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,30]]},"references-count":44,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["metabo12080714"],"URL":"https:\/\/doi.org\/10.3390\/metabo12080714","relation":{},"ISSN":["2218-1989"],"issn-type":[{"value":"2218-1989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,30]]}}}