{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T19:40:26Z","timestamp":1785872426723,"version":"3.56.0"},"reference-count":69,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,16]],"date-time":"2022-08-16T00:00:00Z","timestamp":1660608000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)","award":["#2020\/04730-7"],"award-info":[{"award-number":["#2020\/04730-7"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)","award":["#2017\/12725-0"],"award-info":[{"award-number":["#2017\/12725-0"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>The aim of this study was to carry out a systematic investigation and analysis of different drug extraction methods, specifically non-steroidal anti-inflammatory drugs in biological fluid samples, for Liquid Chromatography in Mass Spectrometry assays (LC-MS\/MS). A search was carried out in the main databases between 1999 and 2021, following the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) checklist. Data were obtained through PubMed, Lilacs, Embase, Scopus, and Web of Science databases using the Boolean operators AND and OR. Studies were pre-selected by title and abstract by two independent reviewers. The selected texts were read in full, and only those that were complete and compatible with the inclusion and exclusion criteria were eligible for this research. A total of 248 references were obtained in the databases. After removing the duplicates and analyzing the titles and abstracts, 79 references were evaluated and passed to the next phase, which comprised the complete reading of the article. A total of 39 publications were eligible for this study. In 52% of the studies, the authors used the liquid\u2013liquid extraction method (LLE), while in 41%, the solid-phase extraction method (SPE) was used. A total of 5% used microextraction methods and 2% used less-conventional techniques. The literature on the main methods used, the LLE and SPE methods, is extensive and consolidated; however, we found other studies that reported modifications of these traditional techniques, which were equally validated for use in LC-MS\/MS. From this review, it is concluded that the diversity of techniques, reliability, and practical information about each analytical method used in this study can be adapted to advances in LC-MS\/MS techniques; however, more ecological, economic, and sustainable approaches should be explored in the future.<\/jats:p>","DOI":"10.3390\/metabo12080751","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T03:15:27Z","timestamp":1660706127000},"page":"751","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Analysis of Different Methods of Extracting NSAIDs in Biological Fluid Samples for LC-MS\/MS Assays: Scoping Review"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2634-8481","authenticated-orcid":false,"given":"Viviane Silva","family":"Siqueira Sandrin","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0990-7106","authenticated-orcid":false,"given":"Gabriela Moraes","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovana Maria","family":"Weckwerth","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nelson Leonel Del Hierro","family":"Polanco","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1vio Augusto Cardoso","family":"Faria","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0405-3500","authenticated-orcid":false,"given":"Carlos Ferreira","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2876-0955","authenticated-orcid":false,"given":"Adriana Maria","family":"Calvo","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"326","DOI":"10.2174\/1389200215666140202214454","article-title":"Prediction by pharmacogenetics of safety and efficacy of non-steroidal anti- inflammatory drugs: A review","volume":"15","author":"Rollason","year":"2014","journal-title":"Curr. 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