{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T16:48:30Z","timestamp":1790959710382,"version":"4.1.0"},"reference-count":41,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,7]],"date-time":"2022-11-07T00:00:00Z","timestamp":1667779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Nature and Science Foundation of Zhejiang Province","award":["LQ20H120004"],"award-info":[{"award-number":["LQ20H120004"]}]},{"name":"Nature and Science Foundation of Zhejiang Province","award":["82101158"],"award-info":[{"award-number":["82101158"]}]},{"DOI":"10.13039\/501100001809","name":"The National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["LQ20H120004"],"award-info":[{"award-number":["LQ20H120004"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"The National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101158"],"award-info":[{"award-number":["82101158"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Retinal detachment is a serious ocular disease leading to photoreceptor degeneration and vision loss. However, the mechanism of photoreceptor degeneration remains unclear. The aim of this study was to investigate the altered metabolism pathway and physiological changes after retinal detachment. Eight-week-old male SD rats were fed, and the model of retinal detachment was established by injecting hyaluronic acid into the retinal space. The rats were euthanized 3 days after RD, and the retinal tissues were sectioned for analysis. Untargeted lipid chromatography-mass spectrometry lipidomic was performed to analyze the metabolite changes. A total of 90 significant metabolites (34 in anionic and 56 in cationic models) were detected after retinal detachment. The main pathways were (1) histidine metabolism; (2) phenylalanine, tyrosine, and tryptophan biosynthesis; and (3) glycine, serine, and threonine metabolism. The key genes corresponding to each metabolic pathway were verified from the Gene Expression Omnibus (GEO) database of human retinal samples. The results indicated that the production of histamine by histidine decarboxylase from histidine reduced after RD (p &lt; 0.05). Xanthine, hypoxanthine, guanine, and guanosine decreased after RD (p &lt; 0.05). Decreased xanthine and hypoxanthine may reduce the antioxidant ability. The decreased guanosine could not provide enough sources for inosine monophosphate production. Tyrosine is an important neurotransmitter and was significantly reduced after RD (p &lt; 0.05). Citrate was significantly reduced with the increase of ATP-citrate lyase enzyme (ACLY) (p &lt; 0.05). We inferred that lipid oxidation might increase rather than lipid biogenesis. Thus, this study highlighted the main changes of metabolite and physiological process after RD. The results may provide important information for photoreceptor degeneration.<\/jats:p>","DOI":"10.3390\/metabo12111077","type":"journal-article","created":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T12:45:47Z","timestamp":1667911547000},"page":"1077","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Metabolomic Study of a Rat Model of Retinal Detachment"],"prefix":"10.3390","volume":"12","author":[{"given":"Xiangjun","family":"She","sequence":"first","affiliation":[{"name":"School of Ophthalmology, Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0980-7129","authenticated-orcid":false,"given":"Yifan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Putuo Poeple\u2019s Hospital, Tongji University, Shanghai 200070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Liang","sequence":"additional","affiliation":[{"name":"School of Ophthalmology, Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Shanghai General Hospital, National Clinical Research Center for Eye Diseases, Shanghai 200080, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bintao","family":"Xie","sequence":"additional","affiliation":[{"name":"School of Ophthalmology, Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China"},{"name":"State Key Laboratory of Optometry, Ophthalmology, and Vision Science, Wenzhou 325027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Ophthalmology, Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Shen","sequence":"additional","affiliation":[{"name":"School of Ophthalmology, Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China"},{"name":"State Key Laboratory of Optometry, Ophthalmology, and Vision Science, Wenzhou 325027, China"},{"name":"Zhejiang Provincial People\u2019s Hospital, Affiliated People\u2019s Hospital of Hangzhou Medical College, Shangtang Road 158#, Gongshu District, Hangzhou 310014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,7]]},"reference":[{"key":"ref_1","first-page":"0710","article-title":"Retinal detachment","volume":"2014","author":"Steel","year":"2014","journal-title":"BMJ Clin. 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