{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T15:44:21Z","timestamp":1766159061763,"version":"build-2065373602"},"reference-count":42,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T00:00:00Z","timestamp":1668038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Metabolic alterations occurring in cancer cells have been seen to also occur in other tissues than cancerous tissue. For instance, cachexia, peripheral insulin resistance, or both are commonly seen in patients with cancer. We explored differences in substrate use in myotubes conditioned with the medium from a pancreatic cancer cell line, PANC-1, or primary human pancreatic cells, hPECs. Protein turnover was assessed using scintillation proximity assay, glucose and oleic acid handling were analyzed by substrate oxidation assay. We performed qPCR to study gene expression and immunoblotting and proteomic analyses to study protein expression. PANC-1-conditioned myotubes had an imbalance in protein turnover with decreased accumulation, increased decay, and decreased MYH2 gene expression. Glucose uptake decreased despite increased insulin-stimulated Akt phosphorylation. Fatty acid uptake increased, whereas fatty acid oxidation was unchanged, leading to accumulation of intracellular lipids (TAG) in PANC-1-conditioned myotubes. Secretome analyses revealed increased release of growth factors and growth factor receptor from PANC-1 cells, potentially affecting muscle cell metabolism. Myotubes exposed to pancreatic cancer cell medium displayed altered energy metabolism with increased protein\/leucine turnover and lipid accumulation, while glucose uptake and oxidation reduced. This indicates production and release of substances from pancreatic cancer cells affecting skeletal muscle.<\/jats:p>","DOI":"10.3390\/metabo12111095","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T19:16:23Z","timestamp":1668107783000},"page":"1095","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Pancreatic Cancer Cell-Conditioned, Human-Derived Primary Myotubes Display Increased Leucine Turnover, Increased Lipid Accumulation, and Reduced Glucose Uptake"],"prefix":"10.3390","volume":"12","author":[{"given":"Solveig A.","family":"Krapf","sequence":"first","affiliation":[{"name":"Section for Pharmacology and Pharmaceutical Biosciences, Department of Pharmacy, University of Oslo, NO-0316 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0744-8268","authenticated-orcid":false,"given":"Jenny","family":"Lund","sequence":"additional","affiliation":[{"name":"Section for Pharmacology and Pharmaceutical Biosciences, Department of Pharmacy, University of Oslo, NO-0316 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Awais Ur Rehman","family":"Saqib","sequence":"additional","affiliation":[{"name":"Section for Pharmacology and Pharmaceutical Biosciences, Department of Pharmacy, University of Oslo, NO-0316 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hege G.","family":"Bakke","sequence":"additional","affiliation":[{"name":"Section for Pharmacology and Pharmaceutical Biosciences, Department of Pharmacy, University of Oslo, NO-0316 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arild C.","family":"Rustan","sequence":"additional","affiliation":[{"name":"Section for Pharmacology and Pharmaceutical Biosciences, Department of Pharmacy, University of Oslo, NO-0316 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. Hege","family":"Thoresen","sequence":"additional","affiliation":[{"name":"Section for Pharmacology and Pharmaceutical Biosciences, Department of Pharmacy, University of Oslo, NO-0316 Oslo, Norway"},{"name":"Department of Immunology, Institute of Clinical Medicine, University of Oslo, NO-0318 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eili T.","family":"Kase","sequence":"additional","affiliation":[{"name":"Section for Pharmacology and Pharmaceutical Biosciences, Department of Pharmacy, University of Oslo, NO-0316 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Adamska, A., Domenichini, A., and Falasca, M. (2017). Pancreatic Ductal Adenocarcinoma: Current and Evolving Therapies. Int. J. Mol. 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