{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:55:06Z","timestamp":1790790906471,"version":"4.1.0"},"reference-count":56,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T00:00:00Z","timestamp":1673222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Michael J. Fox Foundation (USA)","award":["16486"],"award-info":[{"award-number":["16486"]}]},{"name":"Michael J. Fox Foundation (USA)","award":["BB\/P028195\/1"],"award-info":[{"award-number":["BB\/P028195\/1"]}]},{"name":"Michael J. Fox Foundation (USA)","award":["146281"],"award-info":[{"award-number":["146281"]}]},{"name":"Biotechnology and Biological Sciences Research Council","award":["16486"],"award-info":[{"award-number":["16486"]}]},{"name":"Biotechnology and Biological Sciences Research Council","award":["BB\/P028195\/1"],"award-info":[{"award-number":["BB\/P028195\/1"]}]},{"name":"Biotechnology and Biological Sciences Research Council","award":["146281"],"award-info":[{"award-number":["146281"]}]},{"name":"National Institute of Health Research Cambridge Biomedical Research Centre","award":["16486"],"award-info":[{"award-number":["16486"]}]},{"name":"National Institute of Health Research Cambridge Biomedical Research Centre","award":["BB\/P028195\/1"],"award-info":[{"award-number":["BB\/P028195\/1"]}]},{"name":"National Institute of Health Research Cambridge Biomedical Research Centre","award":["146281"],"award-info":[{"award-number":["146281"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>The metabolic basis of Parkinson\u2019s disease pathology is poorly understood. However, the involvement of mitochondrial and endoplasmic reticulum stress in dopamine neurons in disease aetiology is well established. We looked at the effect of rotenone- and tunicamycin-induced mitochondrial and ER stress on the metabolism of wild type and microtubule-associated protein tau mutant dopamine neurons. Dopamine neurons derived from human isolated iPSCs were subjected to mitochondrial and ER stress using RT and TM, respectively. Comprehensive metabolite profiles were generated using a split phase extraction analysed by reversed phase lipidomics whilst the aqueous phase was measured using HILIC metabolomics. Mitochondrial and ER stress were both shown to cause significant dysregulation of metabolism with RT-induced stress producing a larger shift in the metabolic profile of both wild type and MAPT neurons. Detailed analysis showed that accumulation of triglycerides was a significant driver of metabolic dysregulation in response to both stresses in both genotypes. Whilst the consequence is similar, the mechanisms by which triglyceride accumulation occurs in dopamine neurons in response to mitochondrial and ER stress are very different. Thus, improving our understanding of how these mechanisms drive the observed triglyceride accumulation can potentially open up new therapeutic avenues.<\/jats:p>","DOI":"10.3390\/metabo13010112","type":"journal-article","created":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T06:38:27Z","timestamp":1673246307000},"page":"112","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Mitochondrial and Endoplasmic Reticulum Stress Trigger Triglyceride Accumulation in Models of Parkinson\u2019s Disease Independent of Mutations in MAPT"],"prefix":"10.3390","volume":"13","author":[{"given":"Hugo J. R.","family":"Fernandes","sequence":"first","affiliation":[{"name":"Department of Clinical Neurosciences, UK Dementia Research Institute, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0AH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josh P.","family":"Kent","sequence":"additional","affiliation":[{"name":"Department of Clinical Neurosciences, UK Dementia Research Institute, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0AH, UK"},{"name":"Core Metabolomics and Lipidomics Laboratory, Institute of Metabolic Science, University of Cambridge, Level 4 Pathology, Cambridge Biomedical Campus, Cambridge CB2 0QQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Bruntraeger","sequence":"additional","affiliation":[{"name":"Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton CB10 1SA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew R.","family":"Bassett","sequence":"additional","affiliation":[{"name":"Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton CB10 1SA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9998-051X","authenticated-orcid":false,"given":"Albert","family":"Koulman","sequence":"additional","affiliation":[{"name":"Core Metabolomics and Lipidomics Laboratory, Institute of Metabolic Science, University of Cambridge, Level 4 Pathology, Cambridge Biomedical Campus, Cambridge CB2 0QQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanouil","family":"Metzakopian","sequence":"additional","affiliation":[{"name":"Department of Clinical Neurosciences, UK Dementia Research Institute, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0AH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart G.","family":"Snowden","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Royal Holloway University of London, Egham, London TW20 0EX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,9]]},"reference":[{"key":"ref_1","unstructured":"(2021, January 27). Parkinson\u2019s Disease UK. The Incidence and Prevalence of Parkinson\u2019s in the UK: Summary of Findings. Available online: https:\/\/www.parkinsons.org.uk\/professionals\/resources\/incidence-and-prevalence-parkinsons-uk-report."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1016\/S0140-6736(14)61393-3","article-title":"Parkinson\u2019s disease","volume":"386","author":"Kalia","year":"2015","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"S16","DOI":"10.1002\/ana.10487","article-title":"Genetic and environmental factors in the cause of Pakinson\u2019s disease","volume":"53","author":"Warner","year":"2003","journal-title":"Ann. Neurol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1007\/s00439-008-0466-z","article-title":"Exploring gene-environment interactions in Parkinson\u2019s disease","volume":"123","author":"McCulloch","year":"2008","journal-title":"Hum. Genet."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1111\/jnc.13249","article-title":"Structure, function and toxicity of alpha-synuclein: The Bermuda triangle in synucleinopathies","volume":"139","author":"Outeiro","year":"2016","journal-title":"J. Neurochem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.1093\/hmg\/ddz038","article-title":"Cellular \u03b1-synuclein pathology is associated with bioenergetic dysfunction in Parkinson\u2019s iPSC-derived dopamine neurons","volume":"28","author":"Zambon","year":"2019","journal-title":"Hum. Mol. Genet."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.stemcr.2016.01.013","article-title":"ER stress and autophagic perturbations lead to elevated extracellular \u03b1-synuclein in GBA-N370S Parkinson\u2019s iPSC-derived dopamine neurons","volume":"6","author":"Fernandes","year":"2016","journal-title":"Stem Cell Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1007\/s10863-009-9249-z","article-title":"Parkinson\u2019s disease and mitochondrial complex 1: A perspective on the Ndi1therapy","volume":"41","author":"Marella","year":"2009","journal-title":"J. Bioenerg. Biomembr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1111\/j.1471-4159.1990.tb02325.x","article-title":"Mitochondrial complex 1 deficiency in Parkinson\u2019s disease","volume":"54","author":"Schapira","year":"1990","journal-title":"J. Neurochem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"560","DOI":"10.3389\/fnins.2019.00560","article-title":"Linking the endoplasmic reticulum to Parkinson\u2019s disease and alpha-synucleinopathy","volume":"13","author":"Colla","year":"2019","journal-title":"Front. Neurosci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1850","DOI":"10.1091\/mbc.e09-09-0801","article-title":"Alpha-synuclein delays endoplasmic reticulum (ER)-to-Golgi transport in mammalian cells by antagonizing ER\/Golgi SNAREs","volume":"21","author":"Thayanidhi","year":"2010","journal-title":"Mol. Biol. Cell"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1002\/ana.23659","article-title":"Neuropathologic substrates of Parkinson disease dementia","volume":"72","author":"Irwin","year":"2012","journal-title":"Ann. Neurol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s40478-020-00914-9","article-title":"Neuropathological correlates of parkinsonian disorders in large Dutch autopsy series","volume":"8","author":"Geut","year":"2020","journal-title":"Acta Neuropath. Commun."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2958","DOI":"10.1093\/brain\/awp245","article-title":"The distinct cognitive syndromes of Parkinson\u2019s disease: 5 year follow-up of the CamPaIGN cohort","volume":"132","author":"Evans","year":"2009","journal-title":"Brain"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1002\/ana.21192","article-title":"Tau and alpha-synuclein in susceptibility to, and dementia in, Parkinson\u2019s disease","volume":"62","author":"Goris","year":"2007","journal-title":"Ann. Neurol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1136\/jnnp-2013-305277","article-title":"2013. The CamPaIGN study of Parkinson\u2019s disease: 10-year outlook in an incident population-based cohort","volume":"84","author":"Mason","year":"2013","journal-title":"J. Neurol. Neurosurg. Psychiatry"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1038\/81834","article-title":"Chronic systemic pesticide exposure reproduces features of Parkinsons disease","volume":"3","author":"Betarbet","year":"2000","journal-title":"Nat. Neurosci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"10116","DOI":"10.1016\/j.neuro.2014.12.002","article-title":"An update on the rotenone models of Parkinson\u2019s disease: Their ability to reproduce the features of clinical disease and model gene-environment interactions","volume":"46","author":"Johnson","year":"2015","journal-title":"Neurotoxicology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.bbrc.2007.01.043","article-title":"Activation of the unfolded protein response in Parkinson\u2019s disease. Biochem","volume":"354","author":"Hoozemans","year":"2007","journal-title":"Biophys. Res. Commun."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"10690","DOI":"10.1523\/JNEUROSCI.22-24-10690.2002","article-title":"Endoplasmic reticulum stress and the unfolded protein response in cellular models of Parkinson\u2019s disease","volume":"22","author":"Ryu","year":"2002","journal-title":"J. Neurosci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"19367","DOI":"10.1074\/jbc.M211821200","article-title":"Parkinsonian mimetics induce aspects of unfolded protein response in death of dopaminergic neurons","volume":"278","author":"Holtz","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1126\/science.1129462","article-title":"Alpha-synuclein blocks ER-Golgi traffic and Rab1 rescues neuron loss in Parkinson\u2019s models","volume":"313","author":"Cooper","year":"2006","journal-title":"Science"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3306","DOI":"10.1523\/JNEUROSCI.5367-11.2012","article-title":"Endoplasmic reticulum stress is important for the manifestations of \u03b1-synucleinopathy in vivo","volume":"32","author":"Colla","year":"2012","journal-title":"J. Neurosci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1038\/s41467-019-09494-2","article-title":"Felodipine induces autophagy in mouse brains with pharmacokinetics amenable to repurposing","volume":"10","author":"Siddiqi","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1038\/nature10648","article-title":"Dopamine Neurons Derived From Human ES Cells Efficiently Engraft in Animal Models of Parkinson\u2019s Disease","volume":"480","author":"Kriks","year":"2011","journal-title":"Nature"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"108263","DOI":"10.1016\/j.celrep.2020.108263","article-title":"Single cell transcriptomics of Parkinson\u2019s disease human in vitro models reveals Dopamine neuron-specific stress response","volume":"33","author":"Fernandes","year":"2020","journal-title":"Cell Rep."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/978-1-4939-9170-9_11","article-title":"Editing the genome of human induced pluripotent stem cells using CRISPR\/Cas9 ribonucleoprotein complex. CRISPR Gene Editing","volume":"1961","author":"Bruntraeger","year":"2019","journal-title":"Methods Mol. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ebshiana, A.A., Snowden, S.G., Thambisetty, M., Parsons, R., Hye, A., and Legido-Quigley, C. (2015). Metabolomic Method: UPLC-q-ToF Polar and NonPolar Metabolites in the Healthy Rat Cerebellum Using an In-Vial Dual Extraction. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0122883"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1038\/nbt.2377","article-title":"A cross-platform toolkit for mass spectrometry and proteomics","volume":"30","author":"Chambers","year":"2012","journal-title":"Nat. Biotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1021\/ac202450g","article-title":"CAMERA: An integrated strategy for compound spectra extraction and annotation of liquid chromatography\/mass spectrometry datasets","volume":"84","author":"Kuhl","year":"2012","journal-title":"Anal. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1002\/hep.25601","article-title":"Absence of adipose triglyceride lipase protects from hepatic endoplasmic reticulum stress in mice","volume":"56","author":"Fuchs","year":"2012","journal-title":"Hepatology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2749","DOI":"10.1016\/j.biocel.2013.09.011","article-title":"Rotenone induces reductive stress and triacylglycerol deposition in C2C12 cells","volume":"45","author":"He","year":"2013","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.1016\/j.bbalip.2014.09.012","article-title":"Sequestration of fatty acids in triglycerides prevents endoplasmic reticulum stress in an in vitro model of cardiomyocyte lipotoxicity","volume":"1841","author":"Bosma","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.1242\/jcs.02848","article-title":"CREB activation induced by mitochondrial dysfunction triggers triglyceride accumulation in 3T3-L1 preadipocytes","volume":"119","author":"Vankoningsloo","year":"2006","journal-title":"J. Cell Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1194\/jlr.M400464-JLR200","article-title":"Mitochondrial dysfunction induces triglyceride accumulation in 3T3-L1 cells: Role of fatty acid beta-oxidation and glucose","volume":"46","author":"Vankoningsloo","year":"2005","journal-title":"J. Lipid Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.tox.2017.11.010","article-title":"The high-production volume fungicide pyroclostrobin induces triglyceride accumulation associated with mitochondrial dysfunction, and promotes adipocyte differentiation independent of PPAR\u03b3 activation, in 3T3-L1 cells","volume":"15","author":"Luz","year":"2018","journal-title":"Toxicology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1038\/jcbfm.2011.175","article-title":"Brain lactate metabolism: The discoveries and the controversies","volume":"32","author":"Dienel","year":"2012","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1038\/jcbfm.2011.149","article-title":"Sweet sixteen for ANLS","volume":"32","author":"Pellerin","year":"2012","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1038\/jcbfm.2013.128","article-title":"Why does brain metabolism not favour burning of fatty acids to provide energy?\u2014Reflections on disadvantages of the use of free fatty acids as fuel for brain","volume":"33","author":"Schonfeld","year":"2013","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/S0163-7827(03)00051-1","article-title":"Enzymes of triacylglycerol synthesis and their regulation","volume":"43","author":"Coleman","year":"2004","journal-title":"Prog. Lipid Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1038\/nature09968","article-title":"Aberrant lipid metabolism disrupts calcium homeostasis causing liver endoplasmic reticulum stress in obesity","volume":"473","author":"Fu","year":"2011","journal-title":"Nature"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1002\/biof.135","article-title":"Fatty acids and the endoplasmic reticulum in nonalcoholic fatty liver disease","volume":"37","author":"Gentile","year":"2011","journal-title":"BioFactors"},{"key":"ref_43","first-page":"E576","article-title":"Palmitate modulates intracellular signaling, induces endoplasmic reticulum 12 Biochemistry Research International stress, and causes apoptosis in mouse 3T3-L1 and rat primary preadipocytes","volume":"293","author":"Guo","year":"2007","journal-title":"Am. J. Physiol."},{"key":"ref_44","first-page":"E275","article-title":"Saturated fatty acids induce endoplasmic reticulum stress and apoptosis independently of ceramide in liver cells","volume":"291","author":"Wei","year":"2006","journal-title":"Am. J. Physiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"101703","DOI":"10.1016\/j.isci.2020.101703","article-title":"Development and application of high-throughput single cell lipid profiling: A study of SNCA-A53T human dopamine neurons","volume":"23","author":"Snowden","year":"2020","journal-title":"iScience"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"36312","DOI":"10.1074\/jbc.M109.049817","article-title":"DGAT1 expression increases heart triglyceride content but ameliorates lipotoxicity","volume":"284","author":"Liu","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1007\/s00395-013-0369-6","article-title":"Age-dependent effects of high fat-diet on murine left ventricles: Role of palmitate","volume":"108","author":"Aurich","year":"2013","journal-title":"Basic Res. Cardiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2844","DOI":"10.1161\/CIRCULATIONAHA.111.060889","article-title":"Ventricular assist device implantation corrects myocardial lipotoxicity, reverses insulin resistance, and normalizes cardiac metabolism in patients with advanced heart failure","volume":"125","author":"Chokshi","year":"2012","journal-title":"Circulation"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"6240","DOI":"10.1074\/jbc.M111.299115","article-title":"Lipolysis response to endoplasmic reticulum stress in adipose cells","volume":"287","author":"Deng","year":"2012","journal-title":"J. Biol. Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.envpol.2017.05.046","article-title":"Endoplasmic reticulum (ER) stress and cAMP\/PKA pathway mediated Zn-induced hepatic lipolysis","volume":"228","author":"Song","year":"2017","journal-title":"Environ. Pollut."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e52291","DOI":"10.7554\/eLife.52291","article-title":"Misfloded proteins bind and activate death receptor 5 to trigger apoptosis during unresolved endoplasmic reticulum stress","volume":"9","author":"Lam","year":"2020","journal-title":"eLife"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2896","DOI":"10.1523\/JNEUROSCI.4674-06.2007","article-title":"Missorting of tau in neurons causes degeneration of synapses that can be rescued by the kinase MARK2\/Par-1","volume":"27","author":"Thies","year":"2007","journal-title":"J. Neurosci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/s13024-015-0064-1","article-title":"Tau missorting and spastin-induced microtubule disruption in neurodegeneration: Alzheimer disease and hereditary spastic paraplegia","volume":"10","author":"Zempel","year":"2015","journal-title":"Mol. Neurodegener."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"17356","DOI":"10.18632\/oncotarget.7861","article-title":"Tau accumulation impairs mitophagy via increasing mitochondrial membrane potential and reducing mitochondrial Parkin","volume":"7","author":"Hu","year":"2016","journal-title":"Oncotarget"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1038\/s41593-018-0332-9","article-title":"Mitophagy inhibits amyloid-beta and tau pathology and reverses cognitive deficits in models of Alzheimer\u2019s disease","volume":"22","author":"Fang","year":"2019","journal-title":"Nat. Neurosci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e99360","DOI":"10.15252\/embj.201899360","article-title":"Disease-associated tau impairs mitophagy by inhibiting Parkin translocation to mitochondria","volume":"38","author":"Cummins","year":"2019","journal-title":"EMBO J."}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/13\/1\/112\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:04:27Z","timestamp":1760119467000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/13\/1\/112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,9]]},"references-count":56,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,1]]}},"alternative-id":["metabo13010112"],"URL":"https:\/\/doi.org\/10.3390\/metabo13010112","relation":{},"ISSN":["2218-1989"],"issn-type":[{"value":"2218-1989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,9]]}}}