{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T23:30:00Z","timestamp":1790983800009,"version":"4.1.0"},"reference-count":35,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T00:00:00Z","timestamp":1676505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EMORI program","award":["MOBIDOC N\u00b0 2018\/376"],"award-info":[{"award-number":["MOBIDOC N\u00b0 2018\/376"]}]},{"name":"Tunisian Higher Education and Scientific Research Ministry","award":["2018-BALT-1127"],"award-info":[{"award-number":["2018-BALT-1127"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Rosmarinus officinalis L. is a dietary source that produces polyphenols as secondary metabolites. These natural compounds with potent antioxidant abilities are increasingly recommended as a supplement to inhibit oxidative stress. In the current work, we evaluated the impact of the extraction method on the chemical composition of R. officinalis extract, especially on the content of carnosic (CA) and rosmarinic (RA) acids using UPLC-MS-DAD as well as on their antioxidant potency. Four extracts of Tunisian rosemary were obtained from non-conventional extraction techniques:ultrasound-assisted extraction (UAE),supercritical extraction (SFE) and UAE and SFE combined ((UAE-SFE(I), UAE-SFE(II)). The UAE exhibited the best total phenolic compounds (i.e., 85.27 mg GAEg\u22121), the highest content of CAand RA and the strongest antioxidant abilities (i.e., IC50 = 0.13 mg\/mL and EC50 = 0.93 mg\/mL for DPPH scavenging test and iron reducing power ability assay). The evaluation of antioxidant activity of UAE inhuman skin fibroblast (HS-68) cell line was carried out after the induction of oxidative stress. The results determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed a strong protective effect against H2O2oxidative stress induction in cells pretreated with UAE. The obtained results allow us to give new insight about the effect of the extraction method on the chemical composition and biological activities of the extract and the importance of the choice of the most appropriate processing technique to prepare rosemary extract with a high antioxidant potency and protective effect against oxidative stress.<\/jats:p>","DOI":"10.3390\/metabo13020290","type":"journal-article","created":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T04:51:46Z","timestamp":1676523106000},"page":"290","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Impact of the Extraction Method on the Chemical Composition and Antioxidant Potency of Rosmarinus officinalis L. Extracts"],"prefix":"10.3390","volume":"13","author":[{"given":"Nedra","family":"Dhouibi","sequence":"first","affiliation":[{"name":"D\u00e9partement de Chimie, Facult\u00e9 des Sciences, Universit\u00e9 de Monastir, LR21ES04-Chimie de l\u2019Environnement & des Proc\u00e9d\u00e9s Propres, Bvd de l\u2019Environnement, Monastir 5000, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9542-2833","authenticated-orcid":false,"given":"Simona","family":"Manuguerra","sequence":"additional","affiliation":[{"name":"Laboratorio di Biochimica Marina ed Ecotossicologia, Dipartimento di Scienze della Terra e del Mare DiSTeM, Universit\u00e0 degli Studi di Palermo, Via G. Barlotta 4, 91100 Trapani, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1192-6285","authenticated-orcid":false,"given":"Rosaria","family":"Arena","sequence":"additional","affiliation":[{"name":"Laboratorio di Biochimica Marina ed Ecotossicologia, Dipartimento di Scienze della Terra e del Mare DiSTeM, Universit\u00e0 degli Studi di Palermo, Via G. Barlotta 4, 91100 Trapani, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3190-9420","authenticated-orcid":false,"given":"Concetta Maria","family":"Messina","sequence":"additional","affiliation":[{"name":"Laboratorio di Biochimica Marina ed Ecotossicologia, Dipartimento di Scienze della Terra e del Mare DiSTeM, Universit\u00e0 degli Studi di Palermo, Via G. Barlotta 4, 91100 Trapani, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8089-5470","authenticated-orcid":false,"given":"Andrea","family":"Santulli","sequence":"additional","affiliation":[{"name":"Laboratorio di Biochimica Marina ed Ecotossicologia, Dipartimento di Scienze della Terra e del Mare DiSTeM, Universit\u00e0 degli Studi di Palermo, Via G. Barlotta 4, 91100 Trapani, Italy"},{"name":"Istituto di Biologia Marina, Consorzio Universitario della Provincia di Trapani, Via G. Barlotta 4, 91100 Trapani, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seifeddine","family":"Kacem","sequence":"additional","affiliation":[{"name":"AgriLand, Avenue Habib Bourguiba, Tunis 2000, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5833-0518","authenticated-orcid":false,"given":"Hatem","family":"Dhaouadi","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Chimie, Facult\u00e9 des Sciences, Universit\u00e9 de Monastir, LR21ES04-Chimie de l\u2019Environnement & des Proc\u00e9d\u00e9s Propres, Bvd de l\u2019Environnement, Monastir 5000, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0840-1485","authenticated-orcid":false,"given":"Abdelkarim","family":"Mahdhi","sequence":"additional","affiliation":[{"name":"Laboratory of Analysis, Treatment and Valorization of Pollutants of the Environment and Products, Faculty of Pharmacy, University of Monastir, Monastir 5000, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/s00425-019-03327-8","article-title":"Characterization of Sicilian rosemary (Rosmarinus officinalis L.) germplasm through a multidisciplinary approach","volume":"251","author":"Carrubba","year":"2020","journal-title":"Planta"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"323","DOI":"10.22270\/jddt.v9i1.2218","article-title":"Rosmarinus officinalis L.: An update review of its phytochemistry and biological activity","volume":"9","author":"Kompelly","year":"2019","journal-title":"J. Drug Deliv. Ther."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1039\/C7FO01508A","article-title":"Protective effect of rosmarinic acid and carnosic acid against streptozotocin-induced oxidation, glycation, inflammation and microbiota imbalance in diabetic rats","volume":"9","author":"Ou","year":"2018","journal-title":"Food Funct."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Mena, P., Cirlini, M., Tassotti, M., Herrlinger, K.A., Dall\u2019Asta, C., and Del Rio, D. (2016). Phytochemical Profiling of Flavonoids, Phenolic Acids, Terpenoids, and Volatile Fraction of a Rosemary (Rosmarinus officinalis L.) Extract. Molecules, 21.","DOI":"10.3390\/molecules21111576"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.supflu.2012.07.006","article-title":"Supercritical rosemary extracts, their antioxidant activity and effect on hepatic tumor progression","volume":"79","author":"Vicente","year":"2013","journal-title":"J. Supercrit. Fluids"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.ifset.2009.08.013","article-title":"Supercritical carbon dioxide extraction of antioxidant fractions from selected Lamiaceae herbs and their antioxidant capacity","volume":"11","author":"Babovic","year":"2010","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.jfoodeng.2016.12.022","article-title":"CO2-supercritical extraction, hydrodistillation and steam distillation of essential oil of rosemary (Rosmarinus officinalis)","volume":"200","author":"Trejo","year":"2017","journal-title":"J. Food Eng."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.ctcp.2017.11.004","article-title":"Effects of Rosmarinus officinalis L. on memory performance, anxiety, depression, and sleep quality in university students: A randomized clinical trial","volume":"30","author":"Nematolahi","year":"2018","journal-title":"Complement. Ther. Clin. Pract."},{"key":"ref_9","first-page":"107","article-title":"Chemical Composition, Antimicrobial and Analgesic Properties of Rosmarinus officinalis L. from North of Iran","volume":"9","author":"Vahdat","year":"2020","journal-title":"J. Med. Plants By-Prod."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.jff.2015.01.015","article-title":"Anti-inflammatory activity of the basolateral fraction of Caco-2 cells exposed to a rosemary supercritical extract","volume":"13","author":"Arranz","year":"2015","journal-title":"J. Funct. Foods"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.tifs.2015.07.015","article-title":"A novel insight on an ancient aromatic plant: The rosemary (Rosmarinus officinalis L.)","volume":"45","author":"Cavaleiro","year":"2015","journal-title":"Trends Food Sci. Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.jff.2014.10.014","article-title":"Two-step sequential supercritical fluid extracts from rosemary with enhanced anti-proliferative activity","volume":"11","author":"Sullini","year":"2014","journal-title":"J. Funct. Foods"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1016\/j.foodchem.2011.11.138","article-title":"Abietanediterpenoids of Rosmarinus officinalis and their diacylglycerolacyltransferase-inhibitory activity","volume":"132","author":"Cui","year":"2012","journal-title":"Food Chem."},{"key":"ref_14","first-page":"1100","article-title":"Therapeutic effects of rosemary (Rosmarinus officinalis L.) and its active constituents on nervous system disorders","volume":"23","author":"Rahbardar","year":"2020","journal-title":"Iran. J. Basic Med. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1080\/19440049.2019.1678771","article-title":"Antifungal and antiaflatoxigenic activity of rosemary essential oil (Rosmarinus officinalis L.) against Aspergillusflavus","volume":"37","author":"Kohiyama","year":"2020","journal-title":"Food Addit. Contam. Part A"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/j.indcrop.2012.07.069","article-title":"Chemical composition and antibacterial and antioxidant properties of commercial essential oils","volume":"43","author":"Teixeira","year":"2013","journal-title":"Ind. Crops Prod."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.phytochem.2014.12.026","article-title":"Carnosic acid","volume":"115","author":"Dussort","year":"2015","journal-title":"Phytochemistry"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2973","DOI":"10.1016\/j.bbamem.2011.08.011","article-title":"The natural antioxidant rosmarinic acid spontaneously penetrates membranes to inhibit lipid peroxidation in situ","volume":"1808","author":"Fadel","year":"2011","journal-title":"Biochim. Biophys. Acta Biomembr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.fbp.2017.07.011","article-title":"Efficiency of the optimized microwave assisted extractions on the yield, chemical composition and biological activities of Tunisian Rosmarinus officinalis L. essential oil","volume":"105","author":"Farhat","year":"2017","journal-title":"Food Bioprod. Process."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1007\/s12161-012-9536-x","article-title":"Optimization of Focused Ultrasound Extraction (FUSE) and Supercritical Fluid Extraction (SFE) of Citrus Peel Volatile Oils and Antioxidants","volume":"6","author":"Omar","year":"2013","journal-title":"Food Anal. Methods"},{"key":"ref_21","first-page":"98","article-title":"The combined extraction of polyphenols from grape marc: Ultrasound assisted extraction followed by supercritical CO2 extraction of ultrasound-raffinate","volume":"61","author":"Natolino","year":"2014","journal-title":"LWT Food Sci. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1016\/j.indcrop.2017.11.056","article-title":"Supercritical CO2 extraction of Rosmarinus eriocalyx growing in Algeria: Chemical composition and antioxidant activity of extracts and their solid plant materials","volume":"111","author":"Bendif","year":"2018","journal-title":"Ind. Crops Prod."},{"key":"ref_23","first-page":"89","article-title":"Antioksidativna aktivnost ekstrakata Galium mollugo L. dobijenih razli\u010ditim tehnikama ekstrakcije","volume":"67","year":"2013","journal-title":"Hem. Ind."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1590\/1678-457x.27816","article-title":"Sonication enhances quality and antioxidant activity of blueberry juice","volume":"37","author":"Zou","year":"2017","journal-title":"Food Sci. Technol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Dhouibi, N., Manuguerra, S., Arena, R., Mahdhi, A., Messina, C.M., Santulli, A., and Dhaouadi, H. (2020). Screening of antioxidant potentials and bioactive properties of the extracts obtained from two Centaurea L. Species (C. kroumirensis Coss. and C. sicula L. subsp. sicula). Appl. Sci., 10.","DOI":"10.3390\/app10072267"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Messina, C.M., Troia, A., Arena, R., Manuguerra, S., Ioannou, T., Curcuraci, E., Giuseppe, R., Hellio, C., and Santulli, A. (2019). Species-Specific Antioxidant Power and Bioactive Properties of the Extracts Obtained from Wild Mediterranean Calendula Spp. (Asteraceae). Appl. Sci., 9.","DOI":"10.3390\/app9214627"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1080\/14786419.2018.1517121","article-title":"The antioxidant power of horseradish, Armoracia rusticana, underlies antimicrobial and antiradical effects, exerted in vitro","volume":"34","author":"Manuguerra","year":"2020","journal-title":"Nat. Prod. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.lwt.2017.10.055","article-title":"Effects of a natural antioxidant, polyphenol-rich rosemary (Rosmarinus officinalis L.) extract, on lipid stability of plant-derived omega-3 fatty-acid rich oil","volume":"89","author":"Wang","year":"2018","journal-title":"LWT Food Sci. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"112049","DOI":"10.1016\/j.indcrop.2019.112049","article-title":"Deep eutectic solvents applied in the extraction and stabilization of rosemary (Rosmarinus officinalis L.) phenolic compounds","volume":"144","author":"Barbieri","year":"2020","journal-title":"Ind. Crops Prod."},{"key":"ref_30","first-page":"8441","article-title":"Effects of extraction technique and solvent on phytochemicals, antioxidant, and antimicrobial activities of cultivated and wild rosemary (Rosmarinus officinalis L.) from Taounate region","volume":"12","author":"Zeroua","year":"2020","journal-title":"Biointerface Res. Appl. Chem."},{"key":"ref_31","first-page":"59","article-title":"In vitro screening for acetylcholinesterase inhibition and antioxidant potential in different extracts of sage (Salvia officinalis L.) and rosemary (Rosmarinus officinalis L.)","volume":"10","author":"Sharma","year":"2020","journal-title":"J. Biol. Act. Prod. Nat."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.foodchem.2008.01.058","article-title":"Antioxidant activities of rosemary (Rosmarinus officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol","volume":"110","author":"Erkan","year":"2008","journal-title":"Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"de Macedo, L.M., Santos, \u00c9.M.D., Milit\u00e3o, L., Tundisi, L.L., Ataide, J.A., Souto, E.B., and Mazzola, P.G. (2020). Rosemary (Rosmarinus officinalis L., syn Salvia rosmarinus Spenn.) and its topical applications: A review. Plants, 9.","DOI":"10.3390\/plants9050651"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.jfoodeng.2011.09.029","article-title":"Assisted extraction of rosemary antioxidants with green solvents","volume":"109","author":"Visentin","year":"2012","journal-title":"J. Food Eng."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.ultsonch.2004.01.015","article-title":"Potential for the use of ultrasound in the extraction of antioxidants from Rosmarinus officinalis for the food and pharmaceutical industry","volume":"11","author":"Albu","year":"2004","journal-title":"Ultrason. Sonochem"}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/13\/2\/290\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:37:59Z","timestamp":1760121479000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/13\/2\/290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,16]]},"references-count":35,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["metabo13020290"],"URL":"https:\/\/doi.org\/10.3390\/metabo13020290","relation":{},"ISSN":["2218-1989"],"issn-type":[{"value":"2218-1989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,16]]}}}