{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T22:39:18Z","timestamp":1791844758715,"version":"4.3.4"},"reference-count":55,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T00:00:00Z","timestamp":1677196800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100016047","name":"Science Fund of the Republic of Serbia","doi-asserted-by":"publisher","award":["7750295"],"award-info":[{"award-number":["7750295"]}],"id":[{"id":"10.13039\/501100016047","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Cows in the peripartal period undergo changes in thyroid hormones and are susceptible to lipomobilization and\/or oxidative stress. The addition of chestnut tannins as polyphenolic compounds in the diet may improve feed efficiency and prevent oxidative stress-related health disorders in transition cows. However, the relationship between chestnut tannin supplementation and thyroid function, which plays an important role in metabolic regulation, has not been investigated in dairy cows. This study was conducted to investigate the effects of chestnut tannin supplementation during the close-up period on thyroid status and to evaluate the interaction between thyroid hormones and oxidative stress biomarkers in prepartum dairy cows. Forty multiparous Holstein cows were fed either a diet containing chestnut tannins (CNTs, n = 20, 1.96 g chestnut tannins\/kg feed, dry matter) or a non-supplemented diet (CON, n = 20) during the last 25 \u00b1 2 days of gestation. Blood samples were collected on the first day of study (before chestnut tannin supplementation) and d 5 before parturition to measure hormonal and oxidative stress indices. Serum concentrations of T3 (p = 0.04) and T4 (p = 0.05) were higher in CNT cows than in the CON group on day 5 before parturition. Thyroid status of CNT cows was associated with higher serum total antioxidant capacity (T-AOC, p &lt; 0.01), activities of superoxide dismutase (SOD, p = 0.03) and glutathione peroxidase (GPx, p = 0.01), and reduced glutathione concentration (GSH, p = 0.05). Serum thiobarbituric acid reactive substances (TBARS) were lower (p = 0.04) which was associated with lower aspartate aminotransferase (AST, p = 0.02), and lactate dehydrogenase (LDH, p = 0.01) activities in the CNT than in the CON group. Estradiol and progesterone did not differ between CNT and CON cows. Chestnut tannin supplementation improves antioxidant protection, prevents oxidation-reduction processes, reduces the degree of liver cell membrane damage, and protects thyroid tissue from damage, allowing higher T3 and T4 synthesis. Considering the importance of the thyroid hormone status before parturition, mechanisms of thyroid hormone regulation in CNT-supplemented dairy cows require more detailed investigations.<\/jats:p>","DOI":"10.3390\/metabo13030334","type":"journal-article","created":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T02:03:26Z","timestamp":1677204206000},"page":"334","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Dietary Supplementation of Chestnut Tannins in Prepartum Dairy Cows Improves Antioxidant Defense Mechanisms Interacting with Thyroid Status"],"prefix":"10.3390","volume":"13","author":[{"given":"Radi\u0161a","family":"Prodanovi\u0107","sequence":"first","affiliation":[{"name":"Department of Ruminants and Swine Diseases, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9246-212X","authenticated-orcid":false,"given":"Sreten","family":"Nedi\u0107","sequence":"additional","affiliation":[{"name":"Department of Ruminants and Swine Diseases, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Vujanac","sequence":"additional","affiliation":[{"name":"Department of Ruminants and Swine Diseases, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jovan","family":"Bojkovski","sequence":"additional","affiliation":[{"name":"Department of Ruminants and Swine Diseases, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana","family":"Nedi\u0107","sequence":"additional","affiliation":[{"name":"Department of Reproduction, Fertility and Artificial Insemination, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ljubomir","family":"Jovanovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danijela","family":"Kirovski","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6404-397X","authenticated-orcid":false,"given":"Sun\u010dica","family":"Borozan","sequence":"additional","affiliation":[{"name":"Department of General Education, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1740","DOI":"10.3168\/jds.2006-445","article-title":"Plasma Paraoxonase, Health, Inflammatory Conditions, and Liver Function in Transition Dairy Cows","volume":"90","author":"Bionaz","year":"2007","journal-title":"J. Dairy Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1071\/AN14503","article-title":"The Nexus between Nutrient Metabolism, Oxidative Stress and Inflammation in Transition Cows","volume":"54","author":"Sordillo","year":"2014","journal-title":"Anim. Prod. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1292\/jvms.20-0024","article-title":"Changes in Oxidative Stress Parameters in Healthy and Diseased Holstein Cows during the Transition Period in Yamagata Prefecture, Japan","volume":"82","author":"Tsuchiya","year":"2020","journal-title":"J. Vet. Med. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2017","DOI":"10.3168\/jds.S0022-0302(05)72878-2","article-title":"Influence of Body Condition Score on Relationships Between Metabolic Status and Oxidative Stress in Periparturient Dairy Cows","volume":"88","author":"Bernabucci","year":"2005","journal-title":"J. Dairy Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1111\/jpn.12273","article-title":"The Importance of the Oxidative Status of Dairy Cattle in the Periparturient Period: Revisiting Antioxidant Supplementation","volume":"99","author":"Abuelo","year":"2015","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Surai, P.F., Kochish, I.I., Fisinin, V.I., and Juniper, D.T. (2019). Revisiting Oxidative Stress and the Use of Organic Selenium in Dairy Cow Nutrition. Animals, 9.","DOI":"10.3390\/ani9070462"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"5901","DOI":"10.3168\/jds.2013-6904","article-title":"Effects of Chestnut Tannins on Performance and Antioxidative Status of Transition Dairy Cows","volume":"96","author":"Liu","year":"2013","journal-title":"J. Dairy Sci."},{"key":"ref_8","first-page":"137","article-title":"Potential and Challenges of Tannins as an Alternative to In-Feed Antibiotics for Farm Animal Production","volume":"4","author":"Huang","year":"2018","journal-title":"Anim. Nutr.\/Zhongguo Xu Mu Shou Yi Xue Hui"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"609","DOI":"10.2478\/aoas-2020-0077","article-title":"Effects of Chestnut Tannins Supplementation of Prepartum Moderate Yielding Dairy Cows on Metabolic Health, Antioxidant and Colostrum Indices","volume":"21","author":"Borozan","year":"2021","journal-title":"Ann. Anim. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1017\/S1751731107000298","article-title":"Bioactivity of Phytochemicals in Some Lesser-Known Plants and Their Effects and Potential Applications in Livestock and Aquaculture Production Systems","volume":"1","author":"Makkar","year":"2007","journal-title":"Animal"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.anifeedsci.2008.10.011","article-title":"Tannins Determined by Various Methods as Predictors of Methane Production Reduction Potential of Plants by an in Vitro Rumen Fermentation System","volume":"150","author":"Jayanegara","year":"2009","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.anifeedsci.2007.09.013","article-title":"Beneficial and Detrimental Effects of Dietary Condensed Tannins for Sustainable Sheep and Goat Production-Progress and Challenges","volume":"147","author":"Waghorn","year":"2008","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2010","DOI":"10.1002\/jsfa.2577","article-title":"Unravelling the Conundrum of Tannins in Animal Nutrition and Health","volume":"86","year":"2006","journal-title":"J. Sci. Food Agric."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"13","DOI":"10.2135\/cropsci2014.07.0489","article-title":"Condensed Tannins in Sainfoin: Composition, Concentration, and Effects on Nutritive and Feeding Value of Sainfoin Forage","volume":"55","author":"Wang","year":"2015","journal-title":"Crop Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.anifeedsci.2015.08.002","article-title":"Divergence between Purified Hydrolysable and Condensed Tannin Effects on Methane Emission, Rumen Fermentation and Microbial Population in Vitro","volume":"209","author":"Jayanegara","year":"2015","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"61","DOI":"10.17221\/155\/2009-CJFS","article-title":"Scavenging Capacity of Superoxide Radical and Screening of Antimicrobial Activity of Castanea Sativa Mill. Extracts","volume":"28","author":"Vidovic","year":"2010","journal-title":"Czech J. Food Sci."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Kochman, J., Jakubczyk, K., Bargiel, P., and Janda-Milczarek, K. (2021). The Influence of Oxidative Stress on Thyroid Diseases. Antioxidants, 10.","DOI":"10.3390\/antiox10091442"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1515\/CCLM.2010.309","article-title":"Association between Thyroid Hormones, Lipids and Oxidative Stress Biomarkers in Overt Hypothyroidism","volume":"48","author":"Santi","year":"2010","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2016\/6757154","article-title":"Thyroid Hormones, Oxidative Stress, and Inflammation","volume":"2016","author":"Mancini","year":"2016","journal-title":"Mediat. Inflamm."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"R189","DOI":"10.1152\/ajpregu.00395.2016","article-title":"Thyroid Hormone-Stimulated Increases in PGC-1\u03b1 and UCP2 Promote Life History-Specific Endocrine Changes and Maintain a Lipid-Based Metabolism","volume":"312","author":"Martinez","year":"2017","journal-title":"Am. J. Physiol. Integr. Comp. Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1515\/jbcpp-2015-0113","article-title":"Effects of Altered Thyroid States on Oxidative Stress Parameters in Rats","volume":"28","author":"Oktay","year":"2017","journal-title":"J. Basic Clin. Physiol. Pharmacol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1530\/JOE-17-0066","article-title":"Hepatic Thyroid Signaling of Heat-Stressed Late Pregnant and Early Lactating Cows","volume":"234","author":"Weitzel","year":"2017","journal-title":"J. Endocrinol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1530\/eje.1.02192","article-title":"The Effects of Sex-Steroid Administration on the Pituitary-Thyroid Axis in Transsexuals","volume":"155","author":"Bisschop","year":"2006","journal-title":"Eur. J. Endocrinol."},{"key":"ref_24","first-page":"897602","article-title":"Thyroid Hormones Concentrations during the Mid-Dry Period: An Early Indicator of Fatty Liver in Holstein-Friesian Dairy Cows","volume":"2010","author":"Kirovski","year":"2010","journal-title":"J. Thyroid Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1515\/acve-2017-0026","article-title":"Changes in Thyroid Hormones Levels and Metabolism in Dairy Cows around Calving","volume":"67","author":"Fiore","year":"2017","journal-title":"Acta Vet. Brno."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Guan, R., Wang, D., Wang, B., Jiang, L., and Liu, J. (2020). Prognostic Potential of Pre-Partum Blood Biochemical and Immune Variables for Postpartum Mastitis Risk in Dairy Cows. BMC Vet. Res., 16.","DOI":"10.1186\/s12917-020-02314-6"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"307","DOI":"10.5264\/eiyogakuzashi.44.307","article-title":"Studies on Products of Browning Reaction. Antioxidative Activities of Products of Browning Reaction Prepared from Glucosamine","volume":"44","author":"Oyaizu","year":"1986","journal-title":"Jpn. J. Nutr. Diet."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/0003-2697(78)90010-6","article-title":"An Improved Spectrophotometric Assay for Superoxide Dismutase Based on Epinephrine Autoxidation","volume":"90","author":"Sun","year":"1978","journal-title":"Anal. Biochem."},{"key":"ref_29","first-page":"444","article-title":"An Improved Coupled Test Procedure for Glutathione Peroxidase (EC 1-11-1-9-) in Blood","volume":"12","author":"Kremers","year":"1974","journal-title":"Z. Klin. Chem. Klin. Biochem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1093\/clinchem\/41.12.1819","article-title":"Lipid Peroxidation and Antioxidants as Biomarkers of Tissue Damage","volume":"41","author":"Gutteridge","year":"1995","journal-title":"Clin. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"B890","DOI":"10.1093\/gerona\/59.9.B890","article-title":"Malondialdehyde, a Lipoperoxidation-Derived Aldehyde, Can Bring About Secondary Oxidative Damage To Proteins","volume":"59","author":"Traverso","year":"2004","journal-title":"J. Gerontol. Ser. A Biol. Sci. Med. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1660","DOI":"10.1038\/nprot.2013.095","article-title":"Analysis of GSH and GSSG after Derivatization with N-Ethylmaleimide","volume":"8","author":"Giustarini","year":"2013","journal-title":"Nat. Protoc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"113","DOI":"10.4103\/japtr.JAPTR_298_18","article-title":"Levothyroxine Improves Paraoxonase (PON-1) Serum Levels in Patients with Primary Hypothyroidism: Case\u2013Control Study","volume":"9","author":"Hussien","year":"2018","journal-title":"J. Adv. Pharm. Technol. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1055\/s-2007-981457","article-title":"Oxidative Stress and Enzymatic Antioxidant Status in Patients with Hypothyroidism before and after Treatment","volume":"115","author":"Baskol","year":"2007","journal-title":"Exp. Clin. Endocrinol. Diabetes"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Soldado, D., Bessa, R.J.B., and Jer\u00f3nimo, E. (2021). Condensed Tannins as Antioxidants in Ruminants\u2014Effectiveness and Action Mechanisms to Improve Animal Antioxidant Status and Oxidative Stability of Products. Animals, 11.","DOI":"10.3390\/ani11113243"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0377-8401(01)00232-2","article-title":"Microbial Interactions with Tannins: Nutritional Consequences for Ruminants","volume":"91","author":"McSweeney","year":"2001","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"5276","DOI":"10.1093\/jas\/sky404.406","article-title":"Effects of Hydrolyzable Tannin with or without Condensed Tannin on Methane Emissions, Nitrogen Use, and Performance of Beef Cattle Fed a High-Forage Diet","volume":"96","author":"Aboagye","year":"2018","journal-title":"J. Anim. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/jn\/136.1.11","article-title":"Induction of Hepatic Antioxidant Enzymes by Phenolic Acids in Rats Is Accompanied by Increased Levels of Multidrug Resistance-Associated Protein 3 MRNA Expression","volume":"136","author":"Yeh","year":"2006","journal-title":"J. Nutr."},{"key":"ref_39","unstructured":"Haslam, E. (1989). Chemistry and Pharmacology of Natural Products, Cambridge University Press."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1016\/j.toxrep.2018.06.005","article-title":"Toxic Effects of Lambda-Cyhalothrin, on the Rat Thyroid: Involvement of Oxidative Stress and Ameliorative Effect of Ginger Extract","volume":"5","year":"2018","journal-title":"Toxicol. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Be\u0161lo, D., Do\u0161li\u0107, G., Agi\u0107, D., Rastija, V., \u0160peranda, M., Gantner, V., and Lu\u010di\u0107, B. (2022). Polyphenols in Ruminant Nutrition and Their Effects on Reproduction. Antioxidants, 11.","DOI":"10.3390\/antiox11050970"},{"key":"ref_42","first-page":"141","article-title":"Pesticides and Oxidative Stress: A Review","volume":"10","author":"Abdollahi","year":"2004","journal-title":"Med. Sci. Monit."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2016\/8354745","article-title":"NADPH Oxidase Inhibitor Apocynin Attenuates PCB153-Induced Thyroid Injury in Rats","volume":"2016","author":"Abliz","year":"2016","journal-title":"Int. J. Endocrinol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1248\/bpb.35.539","article-title":"Gallotannin Suppresses Calcium Oxalate Crystal Binding and Oxalate-Induced Oxidative Stress in Renal Epithelial Cells","volume":"35","author":"Lee","year":"2012","journal-title":"Biol. Pharm. Bull."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.freeradbiomed.2020.08.019","article-title":"Tannic Acid, a Promising Anti-Photoaging Agent: Evidences of Its Antioxidant and Anti-Wrinkle Potentials, and Its Ability to Prevent Photodamage and MMP-1 Expression in L929 Fibroblasts Exposed to UVB","volume":"160","author":"Nakamura","year":"2020","journal-title":"Free Radic. Biol. Med."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/cbf.1119","article-title":"Oxidative Stress and Serum Paraoxonase Activity in Experimental Hypothyroidism: Effect of Vitamin E Supplementation","volume":"23","author":"Dirican","year":"2005","journal-title":"Cell Biochem. Funct."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.tox.2008.09.026","article-title":"Fipronil-Induced Disruption of Thyroid Function in Rats Is Mediated by Increased Total and Free Thyroxine Clearances Concomitantly to Increased Activity of Hepatic Enzymes","volume":"255","author":"Leghait","year":"2009","journal-title":"Toxicology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1016\/j.fct.2007.09.073","article-title":"The Inhibitory Effect of Tannic Acid on Cytochrome P450 Enzymes and NADPH-CYP Reductase in Rat and Human Liver Microsomes","volume":"46","author":"Yao","year":"2008","journal-title":"Food Chem. Toxicol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1677\/jme.0.0310291","article-title":"Hepatic Gene Expression Patterns in Thyroid Hormone-Treated Hypothyroid Rats","volume":"31","author":"Weitzel","year":"2003","journal-title":"J. Mol. Endocrinol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/0009-8981(82)90360-6","article-title":"Superoxide Dismutase in Extracellular Fluids","volume":"126","author":"Marklund","year":"1982","journal-title":"Clin. Chim. Acta."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/j.freeradbiomed.2017.08.013","article-title":"Extracellular Superoxide Dismutase and Its Role in Cancer","volume":"112","author":"Griess","year":"2017","journal-title":"Free Radic. Biol. Med."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.rvsc.2019.07.016","article-title":"Paraoxonase 1 in Bovine Milk and Blood as Marker of Subclinical Mastitis Caused by Staphylococcus Aureus","volume":"125","author":"Vakanjac","year":"2019","journal-title":"Res. Vet. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.cbi.2015.10.002","article-title":"CaNa2EDTA Chelation Attenuates Cell Damage in Workers Exposed to Lead--A Pilot Study","volume":"242","author":"Borozan","year":"2015","journal-title":"Chem. Biol. Interact."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1186\/s13028-016-0231-6","article-title":"Peripheral Thyroid Hormone Levels and Hepatic Thyroid Hormone Deiodinase Gene Expression in Dairy Heifers on the Day of Ovulation and during the Early Peri-Implantation Period","volume":"58","author":"Meyerholz","year":"2016","journal-title":"Acta Vet. Scand."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/j.nut.2012.11.012","article-title":"Selenium: Significance, and Outlook for Supplementation","volume":"29","author":"Kieliszek","year":"2013","journal-title":"Nutrition"}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/13\/3\/334\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:41:04Z","timestamp":1760121664000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/13\/3\/334"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,24]]},"references-count":55,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["metabo13030334"],"URL":"https:\/\/doi.org\/10.3390\/metabo13030334","relation":{},"ISSN":["2218-1989"],"issn-type":[{"value":"2218-1989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,24]]}}}