{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T09:51:22Z","timestamp":1762249882691,"version":"build-2065373602"},"reference-count":56,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,3,29]],"date-time":"2023-03-29T00:00:00Z","timestamp":1680048000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Innovation Project of Chinese Academy of Agricultural Sciences","award":["CAAS-FRI-06","2021YFD1800700"],"award-info":[{"award-number":["CAAS-FRI-06","2021YFD1800700"]}]},{"name":"National Key Research and Development Project","award":["CAAS-FRI-06","2021YFD1800700"],"award-info":[{"award-number":["CAAS-FRI-06","2021YFD1800700"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>As set in the maximum residue limit regulations of the European Commission, this study aimed to obtain the residual parameters in milk with optimized UPLC-MS\/MS conditions and to determine the conclusive drug withdrawal period to ensure food safety. In this research, an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS\/MS) method was developed to study cefquinome sulfate\u2019s residue elimination in milk and to calculate cefquinome\u2019s withdrawal period. Twelve healthy cows free of endometritis were selected for the experiment. Before using the drug, the vaginal orifice and perineum of each cow was disinfected. One dose of intrauterine perfusion was used for each cow, followed by an additional dose after 72 h. Before administration and 12 h, 18 h, 24 h, 36 h, 42 h, 48 h, 60 h, 66 h, 72 h, 84 h, 90 h, and 96 h after the last dose, milk (10 mL) was gathered from each cow\u2019s teat and pooled. For the measurement of cefquinome concentrations in milk, UPLC-MS\/MS was performed. A calibration curve was generated using linear regression as follows: Y = 250.86X \u2212 102.29, with a correlation coefficient of 0.9996; the limits of detection and the limits of quantitation were 0.1 \u03bcg\u00b7kg\u22121 and 0.2 \u03bcg\u00b7kg\u22121, respectively. The average recovery of cefquinome was 88.60 \u00b1 16.33% at 0.2 \u03bcg\u00b7kg\u22121, 100.95 \u00b1 2.54% at 10 \u03bcg\u00b7kg\u22121, and 97.29 \u00b1 1.77% at 50 \u03bcg\u00b7kg\u22121. For 5 consecutive days at the three spiking levels, the intra and inter-day relative standard deviations (RSD) were 1.28%\u201313.73% and 1.81%\u201318.44%, respectively; the residual amount of cefquinome was less than the maximum residue limit of 20 \u03bcg\u00b7kg\u22121, 36 h after administration; and the residual amount was less than the limit of detection (0.1 \u03bcg\u00b7kg\u22121) 48 h after administration. The withdrawal time of cefquinome in cow\u2019s milk was 39.8 h, as calculated using WTM1.4 software. In terms of clinical practical use, the withdrawal period of milk was temporarily set at 48 h after the administration of the cefquinome sulfate uterus injection to cows, in accordance with the recommended dose and course.<\/jats:p>","DOI":"10.3390\/metabo13040492","type":"journal-article","created":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T01:31:30Z","timestamp":1680139890000},"page":"492","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Elimination of Cefquinome Sulfate Residue in Cow\u2019s Milk after Intrauterine Infusion"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1124-3337","authenticated-orcid":false,"given":"Chunshuang","family":"Liu","sequence":"first","affiliation":[{"name":"National Feed Drug Reference Laboratories, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"Key Laboratory of Animal Antimicrobial Resistance Surveillance, Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"Laboratory of Quality & Safety Risk Assessment for Products on Feed-origin Risk Factor Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7082-748X","authenticated-orcid":false,"given":"Mingyue","family":"Han","sequence":"additional","affiliation":[{"name":"National Feed Drug Reference Laboratories, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"Key Laboratory of Animal Antimicrobial Resistance Surveillance, Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"Laboratory of Quality & Safety Risk Assessment for Products on Feed-origin Risk Factor Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5212-4959","authenticated-orcid":false,"given":"Honglei","family":"Wang","sequence":"additional","affiliation":[{"name":"National Feed Drug Reference Laboratories, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"Key Laboratory of Animal Antimicrobial Resistance Surveillance, Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"Laboratory of Quality & Safety Risk Assessment for Products on Feed-origin Risk Factor Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2474-9978","authenticated-orcid":false,"given":"Xiaojie","family":"Chen","sequence":"additional","affiliation":[{"name":"National Feed Drug Reference Laboratories, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"Key Laboratory of Animal Antimicrobial Resistance Surveillance, Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"Laboratory of Quality & Safety Risk Assessment for Products on Feed-origin Risk Factor Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8532-7210","authenticated-orcid":false,"given":"Yaoxin","family":"Tang","sequence":"additional","affiliation":[{"name":"National Feed Drug Reference Laboratories, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"Key Laboratory of Animal Antimicrobial Resistance Surveillance, Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"Laboratory of Quality & Safety Risk Assessment for Products on Feed-origin Risk Factor Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4077-7087","authenticated-orcid":false,"given":"Daokang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Waters Technology Co., Ltd., Shanghai 201206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiubo","family":"Li","sequence":"additional","affiliation":[{"name":"National Feed Drug Reference Laboratories, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"Key Laboratory of Animal Antimicrobial Resistance Surveillance, Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"Laboratory of Quality & Safety Risk Assessment for Products on Feed-origin Risk Factor Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9779-7321","authenticated-orcid":false,"given":"Yiming","family":"Liu","sequence":"additional","affiliation":[{"name":"National Feed Drug Reference Laboratories, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"Key Laboratory of Animal Antimicrobial Resistance Surveillance, Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"Laboratory of Quality & Safety Risk Assessment for Products on Feed-origin Risk Factor Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,29]]},"reference":[{"key":"ref_1","first-page":"77","article-title":"Uterus of the cow after 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