{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T14:41:03Z","timestamp":1768833663895,"version":"3.49.0"},"reference-count":50,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,4,24]],"date-time":"2023-04-24T00:00:00Z","timestamp":1682294400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["2000368\/Z\/15\/Z"],"award-info":[{"award-number":["2000368\/Z\/15\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"name":"St John\u2019s College, Cambridge","award":["2000368\/Z\/15\/Z"],"award-info":[{"award-number":["2000368\/Z\/15\/Z"]}]},{"DOI":"10.13039\/501100019696","name":"McDonald Institute for Archaeological Research","doi-asserted-by":"crossref","award":["2000368\/Z\/15\/Z"],"award-info":[{"award-number":["2000368\/Z\/15\/Z"]}],"id":[{"id":"10.13039\/501100019696","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Metabolites"],"abstract":"<jats:p>Metabolomic approaches, such as in clinical applications of living individuals, have shown potential use for solving questions regarding the past when applied to archaeological material. Here, we study for the first time the potential of this Omic approach as applied to metabolites extracted from archaeological human dentin. Dentin obtained from micro sampling the dental pulp of teeth of victims and non-victims of Yersinia pestis (plague) from a 6th century Cambridgeshire site are used to evaluate the potential use of such unique material for untargeted metabolomic studies on disease state through liquid chromatography hyphenated to high-resolution mass spectrometry (LC-HRMS). Results show that small molecules of both likely endogenous and exogenous sources are preserved for a range of polar and less polar\/apolar metabolites in archaeological dentin; however, untargeted metabolomic profiles show no clear differentiation between healthy and infected individuals in the small sample analysed (n = 20). This study discusses the potential of dentin as a source of small molecules for metabolomic assays and highlights: (1) the need for follow up research to optimise sampling protocols, (2) the requirements of studies with larger sample numbers and (3) the necessity of more databases to amplify the positive results achievable with this Omic technique in the archaeological sciences.<\/jats:p>","DOI":"10.3390\/metabo13050588","type":"journal-article","created":{"date-parts":[[2023,4,25]],"date-time":"2023-04-25T02:06:35Z","timestamp":1682388395000},"page":"588","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Human Archaeological Dentin as Source of Polar and Less Polar Metabolites for Untargeted Metabolomic Research: The Case of Yersinia pestis"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0588-690X","authenticated-orcid":false,"given":"Diego","family":"Badillo-Sanchez","sequence":"first","affiliation":[{"name":"School of Archaeology and Ancient History, University of Leicester, Leicester LE1 7RH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6583-870X","authenticated-orcid":false,"given":"Donald","family":"Jones","sequence":"additional","affiliation":[{"name":"Leicester Cancer Research Centre, RKCSB, University of Leicester, Leicester LE1 7RH, UK"},{"name":"The Leicester van Geest MultiOmics Facility, University of Leicester, Leicester LE1 7RH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7235-4604","authenticated-orcid":false,"given":"Meriam","family":"Guellil","sequence":"additional","affiliation":[{"name":"Institute of Genomics, University of Tartu, 51010 Tartu, Estonia"},{"name":"Department of Evolutionary Anthropology, University of Vienna, 1030 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7424-2094","authenticated-orcid":false,"given":"Sarah","family":"Inskip","sequence":"additional","affiliation":[{"name":"School of Archaeology and Ancient History, University of Leicester, Leicester LE1 7RH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4158-8296","authenticated-orcid":false,"given":"Christiana","family":"Scheib","sequence":"additional","affiliation":[{"name":"Institute of Genomics, University of Tartu, 51010 Tartu, Estonia"},{"name":"McDonald Institute for Archaeological Research, University of Cambridge, Cambridge CB2 3ER, UK"},{"name":"St. John\u2019s College, University of Cambridge, Cambridge CB2 1TP, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jprot.2017.01.004","article-title":"Comparing Ancient DNA Survival and Proteome Content in 69 Archaeological Cattle Tooth and Bone Samples from Multiple European Sites","volume":"158","author":"Wadsworth","year":"2017","journal-title":"J. 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