{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T03:03:32Z","timestamp":1792551812653,"version":"4.5.0"},"reference-count":91,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,2]],"date-time":"2023-08-02T00:00:00Z","timestamp":1690934400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deputyship for Research and Innovation, \u201cMinistry of Education\u201d in Saudi Arabia","award":["IFKSUOR3-082"],"award-info":[{"award-number":["IFKSUOR3-082"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>The implementation of nanotechnology in the field of plant tissue culture has demonstrated an interesting impact on in vitro plant growth and development. Furthermore, the plant tissue culture accompanying nanoparticles has been showed to be a reliable alternative for the biosynthesis of secondary metabolites. Herein, the effectiveness of zinc oxide nanoparticles (ZnONPs) on the growth of Delonix elata calli, as well as their phytochemical profiles, were investigated. Delonix elata seeds were collected and germinated, and then the plant species was determined based on the PCR product sequence of ITS1 and ITS4 primers. Afterward, the calli derived from Delonix elata seedlings were subjected to 0, 10, 20, 30, 40, and 50 mg\/L of ZnONPs. The ZnONPs were biologically synthesized using the Ricinus communis aqueous leaf extract, which acts as a capping and reducing agent, and zinc nitrate solution. The nanostructures of the biogenic ZnONPs were confirmed using different techniques like UV\u2013visible spectroscopy (UV), zeta potential measurement, Fourier transform infrared spectra (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). Adding 30 mg\/L of ZnONPs to the MS media (containing 2.5 \u00b5M 2,4-D and 1 \u00b5M BAP) resulted in the highest callus fresh weight (5.65 g) compared to the control and other ZnONP treatments. Similarly, more phenolic accumulation (358.85 \u00b5g\/g DW) and flavonoid (112.88 \u00b5g\/g DW) contents were achieved at 30 mg\/L. Furthermore, the high-performance liquid chromatography (HPLC) analysis showed significant increments in gallic acid, quercetin, hesperidin, and rutin in all treated ZnONP calli compared to the control. On the other hand, the gas chromatography and mass spectroscopy (GC-MS) analysis of the calli extracts revealed that nine phytochemical compounds were common among all extracts. Moreover, the most predominant compound found in calli treated with 20, 30, 40, and 50 mg\/L of ZnONPs was bis(2-ethylhexyl) phthalate, with percentage areas of 27.33, 38.68, 22.66, and 17.98%, respectively. The predominant compounds in the control and in calli treated with 10 mg\/L of ZnONPs were octadecanoic acid, 2-propenyl ester and heptanoic acid. In conclusion, in this study, green ZnONPs exerted beneficial effects on Delonix elata calli and improved their production of bioactive compounds, especially at a dose of 30 mg\/L.<\/jats:p>","DOI":"10.3390\/metabo13080905","type":"journal-article","created":{"date-parts":[[2023,8,2]],"date-time":"2023-08-02T11:24:26Z","timestamp":1690975466000},"page":"905","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Improving the Production of Secondary Metabolites via the Application of Biogenic Zinc Oxide Nanoparticles in the Calli of Delonix elata: A Potential Medicinal Plant"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7652-5300","authenticated-orcid":false,"given":"Mohamed","family":"Tarroum","sequence":"first","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norah S.","family":"Alfarraj","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fahad","family":"Al-Qurainy","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0704-8803","authenticated-orcid":false,"given":"Abdulrahman","family":"Al-Hashimi","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salim","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Nadeem","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7362-2578","authenticated-orcid":false,"given":"Abdalrhaman M.","family":"Salih","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3142-7501","authenticated-orcid":false,"given":"Hassan O.","family":"Shaikhaldein","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,2]]},"reference":[{"key":"ref_1","unstructured":"Kumari, R.M., Sharma, N., Arya, G., and Nimesh, S. (2019). Plant Nanobionics: Volume 1, Advances in the Understanding of Nanomaterials Research and Applications, Springer Nature."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"613343","DOI":"10.3389\/fchem.2021.613343","article-title":"Endophytic nanotechnology: An approach to study scope and potential applications","volume":"9","author":"Meena","year":"2021","journal-title":"Front. Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1186\/s12951-018-0408-4","article-title":"\u2018Green\u2019synthesis of metals and their oxide nanoparticles: Applications for environmental remediation","volume":"16","author":"Singh","year":"2018","journal-title":"J. Nanobiotechnol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"8874003","DOI":"10.1155\/2022\/8874003","article-title":"Trends in sustainable green synthesis of silver nanoparticles using agri-food waste extracts and their applications in health","volume":"2022","year":"2022","journal-title":"J. Nanomater."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Del Buono, D., Di Michele, A., Costantino, F., Trevisan, M., and Lucini, L. (2021). Biogenic ZnO nanoparticles synthesized using a novel plant extract: Application to enhance physiological and biochemical traits in maize. Nanomaterials, 11.","DOI":"10.3390\/nano11051270"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Karam, S.T., and Abdulrahman, A.F. (2022). Green Synthesis and Characterization of ZnO Nanoparticles by Using Thyme Plant Leaf Extract. Photonics, 9.","DOI":"10.3390\/photonics9080594"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"110122","DOI":"10.1016\/j.inoche.2022.110122","article-title":"Plant-mediated biosynthesis of zinc oxide nanoparticles from Delonix elata: A promising photocatalyst for crystal violet degradation","volume":"146","author":"Karthik","year":"2022","journal-title":"Inorg. Chem. Commun."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.jcis.2016.03.021","article-title":"Aloe vera extract functionalized zinc oxide nanoparticles as nanoantibiotics against multi-drug resistant clinical bacterial isolates","volume":"472","author":"Ali","year":"2016","journal-title":"J. Colloid Interface Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1016\/j.apt.2015.07.001","article-title":"Plant-extract mediated synthesis of ZnO nanoparticles using Pongamia pinnata and their activity against pathogenic bacteria","volume":"26","author":"Sundrarajan","year":"2015","journal-title":"Adv. Powder Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.jphotobiol.2016.12.011","article-title":"A review on biogenic synthesis of ZnO nanoparticles using plant extracts and microbes: A prospect towards green chemistry","volume":"166","author":"Ahmed","year":"2017","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1016\/j.msec.2018.12.023","article-title":"Synthesis and characterization of Zinc oxide nanoparticles utilizing seed source of Ricinus communis and study of its antioxidant, antifungal and anticancer activity","volume":"97","author":"Shobha","year":"2019","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Emul, E., Asik, M.D., Akcan, R., Kose, K., Uzun, L., Saglam, S., Korkusuz, F., and Saglam, N. (2019). Plant Nanobionics: Volume 1, Advances in the Understanding of Nanomaterials Research and Applications, Springer Nature.","DOI":"10.1007\/978-3-030-12496-0_1"},{"key":"ref_13","unstructured":"Choudhary, R.C., Kumari, S., Kumaraswamy, R., Pal, A., Raliya, R., Biswas, P., and Saharan, V. (2019). Plant Nanobionics: Volume 1, Advances in the Understanding of Nanomaterials Research and Applications, Springer Nature."},{"key":"ref_14","first-page":"79","article-title":"A review of zinc oxide nanoparticles: An evaluation of their synthesis, characterization and ameliorative properties for use in the food, pharmaceutical and cosmetic industries","volume":"11","author":"Nayak","year":"2020","journal-title":"J. Excip. Food Chem."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Garc\u00eda-L\u00f3pez, J.I., Zavala-Garc\u00eda, F., Olivares-S\u00e1enz, E., Lira-Sald\u00edvar, R.H., D\u00edaz Barriga-Castro, E., Ruiz-Torres, N.A., Ramos-Cortez, E., V\u00e1zquez-Alvarado, R., and Ni\u00f1o-Medina, G. (2018). Zinc oxide nanoparticles boosts phenolic compounds and antioxidant activity of Capsicum annuum L. during germination. Agronomy, 8.","DOI":"10.3390\/agronomy8100215"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"19136","DOI":"10.1038\/s41598-021-98607-3","article-title":"Biosynthesis of zinc oxide nanoparticles using Phoenix dactylifera and their effect on biomass and phytochemical compounds in Juniperus procera","volume":"11","author":"Salih","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"24430","DOI":"10.1007\/s11356-019-05676-z","article-title":"Differential growth, nutrition, physiology, and gene expression in Melissa officinalis mediated by zinc oxide and elemental selenium nanoparticles","volume":"26","author":"Babajani","year":"2019","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_18","unstructured":"Guzm\u00e1n, M.G., Hern\u00e1ndez, M.F., Rangel, P.P., and Guzm\u00e1n, A.P.G. (2023). ZnO nanoparticles synthesized by chemical precipitation to increase germination and bioactive compounds in sprouts of Raphanus sa-tivus L.. Agro Product., 2."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1080\/21691401.2019.1596942","article-title":"Biogenic zinc oxide nanoparticles-enhanced biosynthesis of lignans and neolignans in cell suspension cultures of Linum usitatissimum L.","volume":"47","author":"Abbasi","year":"2019","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Zaeem, A., Drouet, S., Anjum, S., Khurshid, R., Younas, M., Blondeau, J.P., Tungmunnithum, D., Giglioli-Guivarc\u2019h, N., Hano, C., and Abbasi, B.H. (2020). Effects of Biogenic Zinc Oxide Nanoparticles on Growth and Oxidative Stress Response in Flax Seedlings vs. In Vitro Cultures: A Comparative Analysis. Biomolecules, 10.","DOI":"10.3390\/biom10060918"},{"key":"ref_21","first-page":"655","article-title":"Antioxidative and Hypoglycemic Effects of Delonix elata and Vachellia farnesiana Leaves Extracts on Streptozotocin-Induced Diabetic Male Rats","volume":"8","author":"Alghamdi","year":"2019","journal-title":"Curr. Sci. Int."},{"key":"ref_22","first-page":"1","article-title":"Delonix elata\u2014A Potent Medicinal Plant: A Review","volume":"5","year":"2013","journal-title":"Int. J. Pharm. Pharm. Sci."},{"key":"ref_23","first-page":"141","article-title":"Anti-inflammatory activity of the leaves and bark of Delonix elata","volume":"17","author":"Rao","year":"1997","journal-title":"Anc. Sci. Life"},{"key":"ref_24","first-page":"193","article-title":"Antibacterial activity of leaf extract of Delonix elata and molecular docking studies of luteolin","volume":"3","author":"Pradeepa","year":"2014","journal-title":"J. Biochem. Technol."},{"key":"ref_25","first-page":"93","article-title":"Development and validation of GC\u2013MS methods for determination of leaf and root of Delonix elata (L.) gamble","volume":"1","author":"Senthilkumar","year":"2014","journal-title":"Int. J. Adv. Res. Biol. Sci."},{"key":"ref_26","first-page":"507851","article-title":"Antioxidant and prophylactic effects of Delonix elata L., stem bark extracts, and flavonoid isolated quercetin against carbon tetrachloride-induced hepatotoxicity in rats","volume":"2014","author":"Pradeepa","year":"2014","journal-title":"BioMed Res. Int."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Oliviero, M., Langellotti, A.L., Russo, G.L., Baselice, M., Donadio, A., Ritieni, A., Graziani, G., and Masi, P. (2022). Use of different organic carbon sources in Cynara cardunculus cells: Effects on biomass productivity and secondary metabolites. Plants, 11.","DOI":"10.3390\/plants11050701"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"962112","DOI":"10.3389\/fpls.2022.962112","article-title":"Biogenic silver nanoparticles improve bioactive compounds in medicinal plant Juniperus procera in vitro","volume":"13","author":"Salih","year":"2022","journal-title":"Front. Plant Sci."},{"key":"ref_29","first-page":"679","article-title":"In vitro studies on Delonix elata L., an endangered medicinal plant","volume":"14","author":"Hegazi","year":"2011","journal-title":"World Appl. Sci. J."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Shafique, S., Jabeen, N., Ahmad, K.S., Irum, S., Anwaar, S., Ahmad, N., Alam, S., Ilyas, M., Khan, T.F., and Hussain, S.Z. (2020). Green fabricated zinc oxide nanoformulated media enhanced callus induction and regeneration dynamics of Panicum virgatum L.. PLoS ONE, 15.","DOI":"10.1101\/2020.03.03.974675"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Al-Qurainy, F., Tarroum, M., Khan, S., Nadeem, M., Gaafar, A.-R.Z., Alansi, S., and Alfarraj, N.S. (2022). Genome estimation and phytochemical compound identification in the leaves and callus of Abrus precatorius: A locally endangered plant from the flora of Saudi Arabia. Plants, 11.","DOI":"10.3390\/plants11040567"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1111\/j.1399-3054.1962.tb08052.x","article-title":"A revised medium for rapid growth and bio assays with tobacco tissue cultures","volume":"15","author":"Murashige","year":"1962","journal-title":"Physiol. Plant."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3010","DOI":"10.1021\/jf0115589","article-title":"Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity","volume":"50","author":"Dewanto","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.foodchem.2005.05.024","article-title":"Antioxidant activities of Sechium edule (Jacq.) Swartz extracts","volume":"97","author":"Ordonez","year":"2006","journal-title":"Food Chem."},{"key":"ref_35","unstructured":"Schulz, S., Biwer, P., Harig, T., Koteska, D., and Schlawis, C. (2020). Comprehensive Natural Products III, Elsevier."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1002\/ps.2272","article-title":"Bioactivity of short-chain aliphatic ketones against adults of the granary weevil, Sitophilus granarius (L.)","volume":"68","author":"Germinara","year":"2012","journal-title":"Pest Manag. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1128\/jb.144.2.481-488.1980","article-title":"Differential effects of ethanol and hexanol on the Escherichia coli cell envelope","volume":"144","author":"Ingram","year":"1980","journal-title":"J. Bacteriol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"267","DOI":"10.4001\/003.022.0224","article-title":"(\u00b1)-2-Hexanol from Pterocarpus indicus leaves as attractant for female Aleurodicus dispersus (Hemiptera: Aleyrodidae)","volume":"22","author":"Zheng","year":"2014","journal-title":"Afr. Entomol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1016\/j.neuroscience.2007.03.001","article-title":"Induction of neurite outgrowth in PC12 cells by the medium-chain fatty acid octanoic acid","volume":"146","author":"Kamata","year":"2007","journal-title":"Neuroscience"},{"key":"ref_40","first-page":"488","article-title":"Priming of plant resistance by natural compounds. Hexanoic acid as a model","volume":"5","author":"Finiti","year":"2014","journal-title":"Front. Plant Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1016\/j.archoralbio.2011.01.011","article-title":"Short- and medium-chain fatty acids exhibit antimicrobial activity for oral microorganisms","volume":"56","author":"Huang","year":"2011","journal-title":"Arch. Oral Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1021\/jf2044586","article-title":"Nematicidal activity of (E,E)-2,4-decadienal and (E)-2-decenal from Ailanthus altissima against Meloidogyne javanica","volume":"60","author":"Caboni","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"856484","DOI":"10.3389\/fnut.2022.856484","article-title":"Changes in functional compounds, volatiles, and antioxidant properties of culinary herb coriander leaves (Coriandrum sativum) stored under red and blue LED light for different storage times","volume":"9","author":"Shoko","year":"2022","journal-title":"Front. Nutr."},{"key":"ref_44","first-page":"141","article-title":"Investigation of antimicrobial activities of nonanoic acid derivatives","volume":"15","author":"Sahin","year":"2006","journal-title":"Fresenius Environ. Bull."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2042","DOI":"10.1111\/pce.13011","article-title":"Effects of discrete bioactive microbial volatiles on plants and fungi","volume":"40","author":"Piechulla","year":"2017","journal-title":"Plant Cell Environ."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e1800498","DOI":"10.1002\/cbdv.201800498","article-title":"Volatile composition and biological activities of the leaf essential oil from Zanthoxylum limoncello grown in Oaxaca, M\u00e9xico","volume":"16","year":"2019","journal-title":"Chem. Biodivers."},{"key":"ref_47","first-page":"32","article-title":"Anti-rice pathogenic microbial activity of Persicaria sp. extracts","volume":"23","author":"Chanprapai","year":"2018","journal-title":"Sci. Technol. Asia"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"425","DOI":"10.28941\/pjwsr.v26i4.875","article-title":"Hexane soluble bioactive components of Chenopodium murale stem","volume":"26","author":"Naqvi","year":"2020","journal-title":"Pak. J. Weed Sci. Res."},{"key":"ref_49","first-page":"260","article-title":"Bio-active compounds analysis and characterization in ethanolic plant extracts of Justicia tranquebariensis L. (Acanthaceae)-using GC-MS","volume":"9","author":"Soosairaj","year":"2016","journal-title":"Int. J. Chem. Tech. Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"224","DOI":"10.5530\/ijper.55.1.25","article-title":"Bioactivity of dodecanoic acid extracted from Geitlerinema sp. TRV57","volume":"55","author":"Renugadevi","year":"2021","journal-title":"Indian J. Pharm. Educ. Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1080\/08927014.2018.1544246","article-title":"Pentadecanal inspired molecules as new anti-biofilm agents against Staphylococcus epidermidis","volume":"34","author":"Ricciardelli","year":"2018","journal-title":"Biofouling"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.jtusci.2015.06.004","article-title":"Bioactivity and phytochemical constituents of marine red seaweeds (Jania rubens, Corallina mediterranea and Pterocladia capillacea)","volume":"10","year":"2016","journal-title":"J. Taibah Univ. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1002\/jobm.202100061","article-title":"Antibacterial activities of hexadecanoic acid methyl ester and green-synthesized silver nanoparticles against multidrug-resistant bacteria","volume":"61","author":"Shaaban","year":"2021","journal-title":"J. Basic Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Ganesan, T., Subban, M., Christopher Leslee, D.B., Kuppannan, S.B., and Seedevi, P. (2022). Structural characterization of n-hexadecanoic acid from the leaves of Ipomoea eriocarpa and its antioxidant and antibacterial activities. Biomass Convers. Biorefinery, 1\u201312.","DOI":"10.1007\/s13399-022-03576-w"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1186\/s43141-021-00151-0","article-title":"Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms","volume":"19","author":"Hanafy","year":"2021","journal-title":"J. Genet. Eng. Biotechnol."},{"key":"ref_56","first-page":"2021","article-title":"GC-MS analysis, collected from kavalkinaru area, tirunelveli district, Tamil nadu, India","volume":"8","author":"Beschi","year":"2021","journal-title":"Eur. J. Mol. Clin. Med."},{"key":"ref_57","first-page":"148","article-title":"Chemical constituents of an Iranian grown Capsicum annuum and their cytotoxic activities evaluation","volume":"9","author":"Koudehi","year":"2020","journal-title":"Org. Med. Chem. Int. J."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Javed, M.R., Salman, M., Tariq, A., Tawab, A., Zahoor, M.K., Naheed, S., Shahid, M., Ijaz, A., and Ali, H. (2022). The Antibacterial and Larvicidal Potential of Bis-(2-Ethylhexyl) Phthalate from Lactiplantibacillus plantarum. Molecules, 27.","DOI":"10.3390\/molecules27217220"},{"key":"ref_59","first-page":"314","article-title":"Antimutagenic bis (2-ethylhexyl) phthalate isolated from octopus (Paraoctopus vulgaris)","volume":"41","author":"Velazquez","year":"2020","journal-title":"Food Sci. Technol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.tifs.2014.09.007","article-title":"Implications of nanotechnology for the agri-food industry: Opportunities, benefits and risks","volume":"40","author":"Handford","year":"2014","journal-title":"Trends Food Sci. Technol."},{"key":"ref_61","first-page":"100457","article-title":"Nanotechnology-Big impact: How nanotechnology is changing the future of agriculture?","volume":"10","author":"Saritha","year":"2022","journal-title":"J. Agric. Food Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"180824","DOI":"10.1098\/rsos.180824","article-title":"Castor oil: A suitable green source of capping agent for nanoparticle syntheses and facile surface functionalization","volume":"5","author":"Mensah","year":"2018","journal-title":"R. Soc. Open Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.biotechadv.2013.01.003","article-title":"Synthesis of metallic nanoparticles using plant extracts","volume":"31","author":"Mittal","year":"2013","journal-title":"Biotechnol. Adv."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1049\/iet-nbt.2017.0094","article-title":"Optimised synthesis of ZnO-nano-fertiliser through green chemistry: Boosted growth dynamics of economically important L. esculentum","volume":"12","author":"Jabeen","year":"2018","journal-title":"IET Nanobiotechnology"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"9709","DOI":"10.1021\/acsomega.1c00310","article-title":"Green synthesis of zinc oxide (ZnO) nanoparticles using aqueous fruit extracts of Myristica fragrans: Their characterizations and biological and environmental applications","volume":"6","author":"Faisal","year":"2021","journal-title":"ACS Omega"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"106659","DOI":"10.1016\/j.jece.2021.106659","article-title":"Green synthesis of zinc oxide nanoparticles using Cinnamomum camphora (L.) Presl leaf extracts and its antifungal activity","volume":"9","author":"Zhu","year":"2021","journal-title":"J. Environ. Chem. Eng."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Alenezi, N.A., Al-Qurainy, F., Tarroum, M., Nadeem, M., Khan, S., Salih, A.M., Shaikhaldein, H.O., Alfarraj, N.S., Gaafar, A.-R.Z., and Al-Hashimi, A. (2022). Zinc Oxide Nanoparticles (ZnO NPs), Biosynthesis, Characterization and Evaluation of Their Impact to Improve Shoot Growth and to Reduce Salt Toxicity on Salvia officinalis In Vitro Cultivated. Processes, 10.","DOI":"10.3390\/pr10071273"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Rivero-Montejo, S.d.J., Vargas-Hernandez, M., and Torres-Pacheco, I. (2021). Nanoparticles as novel elicitors to improve bioactive compounds in plants. Agriculture, 11.","DOI":"10.3390\/agriculture11020134"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"109844","DOI":"10.1016\/j.msec.2019.109844","article-title":"A facile green approach of ZnO NRs synthesized via Ricinus communis L. leaf extract for Biological activities","volume":"103","author":"Chennimalai","year":"2019","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"801620","DOI":"10.3389\/fnano.2021.801620","article-title":"Role of biogenic capping agents in the synthesis of metallic nanoparticles and evaluation of their therapeutic potential","volume":"3","author":"Sidhu","year":"2022","journal-title":"Front. Nanotechnol."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Al-Mur, B.A. (2023). Green Zinc Oxide (ZnO) Nanoparticle Synthesis Using Mangrove Leaf Extract from Avicenna marina: Properties and Application for the Removal of Toxic Metal Ions (Cd2+ and Pb2+). Water, 15.","DOI":"10.3390\/w15030455"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"778","DOI":"10.3389\/fchem.2020.00778","article-title":"Microbial fabrication of zinc oxide nanoparticles and evaluation of their antimicrobial and photocatalytic properties","volume":"8","author":"Jain","year":"2020","journal-title":"Front. Chem."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"4786227","DOI":"10.1155\/2021\/4786227","article-title":"Plant-based synthesis of zinc oxide nanoparticles (ZnO-NPs) using aqueous leaf extract of aquilegia pubiflora: Their antiproliferative activity against HepG2 cells inducing reactive oxygen species and other in vitro properties","volume":"2021","author":"Jan","year":"2021","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_74","first-page":"137","article-title":"FTIR spectroscopic studies on cleome gynandra-Comparative analysis of functional group before and after extraction","volume":"22","author":"Lingegowda","year":"2012","journal-title":"Rom. J. Biophys."},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Mansour, A.T., Alprol, A.E., Abualnaja, K.M., El-Beltagi, H.S., Ramadan, K.M.A., and Ashour, M. (2022). The Using of Nanoparticles of Microalgae in Remediation of Toxic Dye from Industrial Wastewater: Kinetic and Isotherm Studies. Materials, 15.","DOI":"10.3390\/ma15113922"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1007\/s12668-020-00802-5","article-title":"Biofabrication of zinc oxide nanoparticles from two different zinc sources and their antimicrobial activity","volume":"11","author":"Khajuria","year":"2021","journal-title":"BioNanoScience"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"3414","DOI":"10.1039\/C8NR09666J","article-title":"The formation and detection techniques of oxygen vacancies in titanium oxide-based nanostructures","volume":"11","author":"Sarkar","year":"2019","journal-title":"Nanoscale"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"100618","DOI":"10.1016\/j.mtchem.2021.100618","article-title":"Anticancer therapeutic efficacy of biogenic Am-ZnO nanoparticles on 2D and 3D tumor models","volume":"22","author":"Chabattula","year":"2021","journal-title":"Mater. Today Chem."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"29541","DOI":"10.1039\/C9RA04424H","article-title":"Optical, morphological and biological analysis of zinc oxide nanoparticles (ZnO NPs) using Papaver somniferum L.","volume":"9","author":"Muhammad","year":"2019","journal-title":"RSC Adv."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"36492","DOI":"10.1039\/C7RA07025J","article-title":"Nanomaterials in plant tissue culture: The disclosed and undisclosed","volume":"7","author":"Kim","year":"2017","journal-title":"RSC Adv."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Alfarraj, N.S., Tarroum, M., Al-Qurainy, F., Nadeem, M., Khan, S., Salih, A.M., Shaikhaldein, H.O., Al-Hashimi, A., Alansi, S., and Perveen, K. (2023). Biosynthesis of Silver Nanoparticles and Exploring Their Potential of Reducing the Contamination of the In Vitro Culture Media and Inducing the Callus Growth of Rumex nervosus Explants. Molecules, 28.","DOI":"10.3390\/molecules28093666"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s11240-020-01920-4","article-title":"Silver nanoparticles induce genetic, biochemical, and phenotype variation in chrysanthemum","volume":"143","author":"Tymoszuk","year":"2020","journal-title":"Plant Cell Tissue Organ Cult. PCTOC"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"723","DOI":"10.2298\/ABS151105017A","article-title":"Impact of application of zinc oxide nanoparticles on callus induction, plant regeneration, element content and antioxidant enzyme activity in tomato (Solanum lycopersicum Mill.) under salt stress","volume":"68","author":"Alharby","year":"2017","journal-title":"Arch. Biol. Sci."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Regni, L., Del Buono, D., Micheli, M., Facchin, S.L., Tolisano, C., and Proietti, P. (2022). Effects of biogenic ZnO nanoparticles on growth, physiological, biochemical traits and antioxidants on olive tree in vitro. Horticulturae, 8.","DOI":"10.3390\/horticulturae8020161"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1080\/21691401.2021.1926274","article-title":"Green and chemically synthesized zinc oxide nanoparticles: Effects on in-vitro seedlings and callus cultures of Silybum marianum and evaluation of their antimicrobial and anticancer potential","volume":"49","author":"Saeed","year":"2021","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1007\/s11240-019-01725-0","article-title":"In vitro effect of zinc oxide nanoparticles on Nicotiana tabacum callus compared to ZnO micro particles and zinc sulfate (ZnSO4)","volume":"140","author":"Dayani","year":"2020","journal-title":"Plant Cell Tissue Organ Cult. PCTOC"},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Park, J.-S., Seong, Z.-K., Kim, M.-S., Ha, J.-H., Moon, K.-B., Lee, H.-J., Lee, H.-K., Jeon, J.-H., Park, S.U., and Kim, H.-S. (2020). Production of flavonoids in callus cultures of Sophora flavescens Aiton. Plants, 9.","DOI":"10.3390\/plants9060688"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1080\/21691401.2021.1946069","article-title":"Interactive effects of zinc oxide nano particles and different light regimes on growth and silymarin biosynthesis in callus cultures of Silybum marianum L.","volume":"49","author":"Shehzad","year":"2021","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1108186","DOI":"10.3389\/fpls.2023.1108186","article-title":"Effect of zinc oxide nanoparticles synthesized from Carya illinoinensis leaf extract on growth and antioxidant properties of mustard (Brassica juncea)","volume":"14","author":"Geremew","year":"2023","journal-title":"Front. Plant Sci."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1007\/s11240-021-02123-1","article-title":"Iron-doped zinc oxide nanoparticles-triggered elicitation of important phenolic compounds in cell cultures of Fagonia indica","volume":"147","author":"Khan","year":"2021","journal-title":"Plant Cell Tissue Organ Cult. PCTOC"},{"key":"ref_91","doi-asserted-by":"crossref","unstructured":"Abdelbaky, A.S., Abd El-Mageed, T.A., Babalghith, A.O., Selim, S., and Mohamed, A.M. (2022). Green synthesis and characterization of ZnO nanoparticles using Pelargonium odoratissimum (L.) aqueous leaf extract and their antioxidant, antibacterial and anti-inflammatory activities. Antioxidants, 11.","DOI":"10.3390\/antiox11081444"}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/13\/8\/905\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:24:38Z","timestamp":1760127878000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/13\/8\/905"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,2]]},"references-count":91,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["metabo13080905"],"URL":"https:\/\/doi.org\/10.3390\/metabo13080905","relation":{},"ISSN":["2218-1989"],"issn-type":[{"value":"2218-1989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,2]]}}}