{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T14:56:03Z","timestamp":1789570563926,"version":"build-2803163510"},"reference-count":36,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T00:00:00Z","timestamp":1692316800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Major Project in Henan Province","award":["221100320200"],"award-info":[{"award-number":["221100320200"]}]},{"name":"Science and Technology Major Project in Henan Province","award":["2003048"],"award-info":[{"award-number":["2003048"]}]},{"name":"Science and Technology Development Plan of Kaifeng in 2020","award":["221100320200"],"award-info":[{"award-number":["221100320200"]}]},{"name":"Science and Technology Development Plan of Kaifeng in 2020","award":["2003048"],"award-info":[{"award-number":["2003048"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Luria\u2013Bertani broth (LB) culture medium is a commonly used bacterial culture medium in the laboratory. The nutrient composition, concentration, and culture conditions of LB medium can influence the growth of microbial strains. The purpose of this article is to demonstrate the impact of LB liquid culture medium on microbial growth under different sterilization conditions. In this study, LB medium with four different treatments was used, as follows: A, LB medium without treatments; B, LB medium with filtration; C, LB medium with autoclaving; and D, LB medium with autoclaving and cultured for 12 h. Subsequently, the protein levels and antioxidant capacity of the medium with different treatments were measured, and the effects of the different LB medium treatments on the growth of microorganisms and metabolites were determined via 16s rRNA gene sequencing and metabolomics analysis, respectively. Firmicutes and Lactobacillus were the dominant microorganisms, which were enriched in fermentation and chemoheterotrophy. The protein levels and antioxidant capacity of the LB medium with different treatments were different, and with the increasing concentration of medium, the protein levels were gradually increased, while the antioxidant capacity was decreased firstly and then increased. The growth trend of Bacillus subtilis, Bacillus paralicheniformis, Micrococcus luteus, and Alternaria alternata in the medium with different treatments was similar. Additionally, 220 and 114 differential metabolites were found between B and C medium, and between C and D medium, which were significantly enriched in the \u201cHedgehog signaling pathway\u201d, \u201cbiosynthesis of plant secondary metabolites\u201d, \u201cABC transporters\u201d, \u201carginine and proline metabolism\u201d, and \u201clinoleic acid metabolism\u201d. LB medium may be a good energy source for Lactobacillus growth with unsterilized medium, and LB medium filtered with a 0.22 \u03bcm filter membrane may be used for bacterial culture better than culture medium after high-pressure sterilization. LB medium still has the ability for antioxidation and to keep bacteria growth whether or not autoclaved, indicating that there are some substances that can resist a high temperature and pressure and still maintain their functions.<\/jats:p>","DOI":"10.3390\/metabo13080958","type":"journal-article","created":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T10:29:25Z","timestamp":1692354565000},"page":"958","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["The Metabolomics Changes in Luria\u2013Bertani Broth Medium under Different Sterilization Methods and Their Effects on Bacillus Growth"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2720-0283","authenticated-orcid":false,"given":"Haifeng","family":"Wang","sequence":"first","affiliation":[{"name":"School of Environmental Engineering, Yellow River Conservancy Technical Institute, Kaifeng Key Laboratory of Food Composition and Quality Assessment, Kaifeng 475004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Environmental Engineering, Yellow River Conservancy Technical Institute, Kaifeng Key Laboratory of Food Composition and Quality Assessment, Kaifeng 475004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Environmental Engineering, Yellow River Conservancy Technical Institute, Kaifeng Key Laboratory of Food Composition and Quality Assessment, Kaifeng 475004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxuan","family":"He","sequence":"additional","affiliation":[{"name":"National Research Center for Wildlife-Borne Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1007\/s12639-016-0786-1","article-title":"LB broth-lyophilized Rabbit serum (LLR) as a new and suitable culture medium for cultivation of promastigotes of Leishmania major","volume":"41","author":"Nasiri","year":"2017","journal-title":"J. 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