{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T20:45:05Z","timestamp":1790196305237,"version":"4.1.0"},"reference-count":51,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T00:00:00Z","timestamp":1692489600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Approximately 25% of psoriasis patients have an inflammatory arthritis termed psoriatic arthritis (PsA). There is strong interest in identifying and validating biomarkers that can accurately and reliably predict conversion from psoriasis to PsA using novel technologies such as metabolomics. Lipids, in particular, are of key interest in psoriatic disease. We sought to develop a liquid chromatography-mass spectrometry (LC-MS) method to be used in conjunction with solid-phase microextraction (SPME) for analyzing fatty acids and similar molecules. A total of 25 chromatographic methods based on published lipid studies were tested on two LC columns. As a proof of concept, serum samples from psoriatic disease patients (n = 27 psoriasis and n = 26 PsA) were processed using SPME and run on the selected LC-MS method. The method that was best for analyzing fatty acids and fatty acid-like molecules was optimized and applied to serum samples. A total of 18 tentatively annotated features classified as fatty acids and other lipid compounds were statistically significant between psoriasis and PsA groups using both multivariate and univariate approaches. The SPME-LC-MS method developed and optimized was capable of detecting fatty acids and similar lipids that may aid in differentiating psoriasis and PsA patients.<\/jats:p>","DOI":"10.3390\/metabo13080963","type":"journal-article","created":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T01:49:34Z","timestamp":1692582574000},"page":"963","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A Solid-Phase Microextraction\u2014Liquid Chromatography-Mass Spectrometry Method for Analyzing Serum Lipids in Psoriatic Disease"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8288-0907","authenticated-orcid":false,"given":"John","family":"Koussiouris","sequence":"first","affiliation":[{"name":"Schroeder Arthritis Institute, Krembil Research Institute, University Health Network, Toronto, ON M5T 0S8, Canada"},{"name":"Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikita","family":"Looby","sequence":"additional","affiliation":[{"name":"Schroeder Arthritis Institute, Krembil Research Institute, University Health Network, Toronto, ON M5T 0S8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vathany","family":"Kulasingam","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada"},{"name":"Division of Clinical Biochemistry, Laboratory Medicine Program, University Health Network, Toronto, ON M5G 2C4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinod","family":"Chandran","sequence":"additional","affiliation":[{"name":"Schroeder Arthritis Institute, Krembil Research Institute, University Health Network, Toronto, ON M5T 0S8, Canada"},{"name":"Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada"},{"name":"Division of Rheumatology, Department of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada"},{"name":"Institute of Medical Science, University of Toronto, Toronto, ON M5S 1A8, Canada"},{"name":"Department of Medicine, Memorial University, St. John\u2019s, NL A1B 3V6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.12688\/f1000research.19144.1","article-title":"Psoriatic arthritis","volume":"8","author":"Ocampo","year":"2019","journal-title":"F1000Res"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.jaad.2018.06.027","article-title":"Prevalence of psoriatic arthritis in patients with psoriasis: A systematic review and meta-analysis of observational and clinical studies","volume":"80","author":"Alinaghi","year":"2019","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s13075-020-2103-8","article-title":"Assessing structural damage progression in psoriatic arthritis and its role as an outcome in research","volume":"22","author":"Gladman","year":"2020","journal-title":"Arthritis Res. Ther."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"FitzGerald, O., and Gladman, D.D. (2018). Oxford Textbook of Psoriatic Arthritis, Oxford Academic.","DOI":"10.1093\/med\/9780198737582.001.0001"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1729","DOI":"10.1002\/acr.20627","article-title":"Cardiovascular and other comorbidities in patients with psoriatic arthritis: A comparison with patients with psoriasis","volume":"63","author":"Husted","year":"2011","journal-title":"Arthritis Care Res."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Koussiouris, J., Looby, N., Anderson, M., Kulasingam, V., and Chandran, V. (2021). Metabolomics Studies in Psoriatic Disease: A Review. Metabolites, 11.","DOI":"10.3390\/metabo11060375"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1080\/1744666X.2020.1752667","article-title":"Pathway to biomarker discovery in psoriatic arthritis","volume":"16","author":"Chandran","year":"2020","journal-title":"Expert Rev. Clin. Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1002\/mas.21562","article-title":"Challenges and emergent solutions for LC-MS\/MS based untargeted metabolomics in diseases","volume":"37","author":"Cui","year":"2018","journal-title":"Mass Spectrom. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"6728","DOI":"10.1039\/C8CC02592D","article-title":"Improving metabolome coverage and data quality: Advancing metabolomics and lipidomics for biomarker discovery","volume":"54","author":"Vuckovic","year":"2018","journal-title":"Chem. Commun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/s11306-021-01805-3","article-title":"Serum metabolic fingerprinting of psoriasis and psoriatic arthritis patients using solid-phase microextraction-liquid chromatography-high-resolution mass spectrometry","volume":"17","author":"Looby","year":"2021","journal-title":"Metabolomics"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/978-1-4939-2377-9_13","article-title":"The strengths and weaknesses of NMR spectroscopy and mass spectrometry with particular focus on metabolomics research","volume":"1277","author":"Emwas","year":"2015","journal-title":"Methods Mol. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1038\/nrd.2016.32","article-title":"Emerging applications of metabolomics in drug discovery and precision medicine","volume":"15","author":"Wishart","year":"2016","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_13","first-page":"5276240","article-title":"Serum Lipidomics Profiling to Identify Biomarkers for Non-Small Cell Lung Cancer","volume":"2018","author":"Chen","year":"2018","journal-title":"BioMed Res. Int."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e4781","DOI":"10.1002\/jms.4781","article-title":"Targeted fatty acid metabolomics to discover Parkinson\u2032s disease associated metabolic alteration","volume":"56","author":"Zhang","year":"2021","journal-title":"J. Mass Spectrom."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1007\/s00216-012-6039-y","article-title":"Current trends and challenges in sample preparation for global metabolomics using liquid chromatography\u2013mass spectrometry","volume":"403","author":"Vuckovic","year":"2012","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.trac.2018.02.005","article-title":"Recent advances in biological sample preparation methods coupled with chromatography, spectrometry and electrochemistry analysis techniques","volume":"102","author":"Niu","year":"2018","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.chroma.2007.10.026","article-title":"Sample preparation","volume":"1184","author":"Chen","year":"2008","journal-title":"J. Chromatogr. A"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.chroma.2013.09.084","article-title":"Microextraction versus exhaustive extraction approaches for simultaneous analysis of compounds in wide range of polarity","volume":"1316","author":"Mirnaghi","year":"2013","journal-title":"J. Chromatogr. A"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1038\/nprot.2009.179","article-title":"Protocol for solid-phase microextraction method development","volume":"5","author":"Risticevic","year":"2010","journal-title":"Nat. Protoc."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Liu, J.W., Murtada, K., Reyes-Garces, N., and Pawliszyn, J. (2020). Systematic Evaluation of Different Coating Chemistries Used in Thin-Film Microextraction. Molecules, 25.","DOI":"10.3390\/molecules25153448"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.chroma.2016.02.054","article-title":"Comprehensive untargeted lipidomic analysis using core-shell C30 particle column and high field orbitrap mass spectrometer","volume":"1440","author":"Zhang","year":"2016","journal-title":"J. Chromatogr. A"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.aca.2016.12.003","article-title":"Off-line mixed-mode liquid chromatography coupled with reversed phase high performance liquid chromatography-high resolution mass spectrometry to improve coverage in lipidomics analysis","volume":"954","author":"Vu","year":"2017","journal-title":"Anal. Chim. Acta"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3749","DOI":"10.1021\/acschemneuro.0c00274","article-title":"Comprehensive Investigation of Metabolic Changes Occurring in the Rat Brain Hippocampus after Fluoxetine Administration Using Two Complementary In Vivo Techniques: Solid Phase Microextraction and Microdialysis","volume":"11","author":"Boyaci","year":"2020","journal-title":"ACS Chem. Neurosci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.chemphyslip.2019.03.006","article-title":"Rational selection of reverse phase columns for high throughput LC-MS lipidomics","volume":"221","author":"Criscuolo","year":"2019","journal-title":"Chem. Phys. Lipids"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.chroma.2016.07.017","article-title":"Analysis of liposoluble carboxylic acids metabolome in human serum by stable isotope labeling coupled with liquid chromatography-mass spectrometry","volume":"1460","author":"Zhu","year":"2016","journal-title":"J. Chromatogr. A"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3192","DOI":"10.1021\/ac103093w","article-title":"Improved LC-MS method for the determination of fatty acids in red blood cells by LC-orbitrap MS","volume":"83","author":"Li","year":"2011","journal-title":"Anal. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1007\/s00216-017-0768-x","article-title":"Two complementary reversed-phase separations for comprehensive coverage of the semipolar and nonpolar metabolome","volume":"410","author":"Naser","year":"2018","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_28","first-page":"3940","article-title":"Identification of psoriasis vulgaris biomarkers in human plasma by non-targeted metabolomics based on UPLC-Q-TOF\/MS","volume":"23","author":"Li","year":"2019","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1021\/pr100885w","article-title":"Metabonomic investigation of liver profiles of nonpolar metabolites obtained from alcohol-dosed rats and mice using high mass accuracy MSn analysis","volume":"10","author":"Loftus","year":"2011","journal-title":"J. Proteome Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.1016\/j.jid.2018.02.003","article-title":"Bioactive Lipid Mediator Profiles in Human Psoriasis Skin and Blood","volume":"138","author":"Sorokin","year":"2018","journal-title":"J. Investig. Dermatol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.chroma.2017.06.017","article-title":"Highly informative multiclass profiling of lipids by ultra-high performance liquid chromatography\u2014Low resolution (quadrupole) mass spectrometry by using electrospray ionization and atmospheric pressure chemical ionization interfaces","volume":"1509","author":"Beccaria","year":"2017","journal-title":"J. Chromatogr. A"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"754","DOI":"10.7150\/thno.51154","article-title":"Metabolomic profiling reveals amino acid and carnitine alterations as metabolic signatures in psoriasis","volume":"11","author":"Chen","year":"2021","journal-title":"Theranostics"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2228","DOI":"10.1021\/acs.jproteome.6b00198","article-title":"High-Throughput Quantitative Lipidomics Analysis of Nonesterified Fatty Acids in Human Plasma","volume":"15","author":"Christinat","year":"2016","journal-title":"J. Proteome Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"12022","DOI":"10.1021\/ac502627w","article-title":"Application of solid phase microextraction for quantitation of polyunsaturated fatty acids in biological fluids","volume":"86","author":"Birjandi","year":"2014","journal-title":"Anal. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"6018","DOI":"10.1021\/ac2010185","article-title":"Optimization of the coating procedure for a high-throughput 96-blade solid phase microextraction system coupled with LC-MS\/MS for analysis of complex samples","volume":"83","author":"Mirnaghi","year":"2011","journal-title":"Anal. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1038\/nbt.2377","article-title":"A cross-platform toolkit for mass spectrometry and proteomics","volume":"30","author":"Chambers","year":"2012","journal-title":"Nat. Biotechnol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"W388","DOI":"10.1093\/nar\/gkab382","article-title":"MetaboAnalyst 5.0: Narrowing the gap between raw spectra and functional insights","volume":"49","author":"Pang","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3156","DOI":"10.1021\/acs.analchem.7b04424","article-title":"METLIN: A Technology Platform for Identifying Knowns and Unknowns","volume":"90","author":"Guijas","year":"2018","journal-title":"Anal. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/j.bbalip.2011.06.009","article-title":"Lipid classification, structures and tools","volume":"1811","author":"Fahy","year":"2011","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"D521","DOI":"10.1093\/nar\/gkl923","article-title":"HMDB: The Human Metabolome Database","volume":"35","author":"Wishart","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1038\/nbt.3597","article-title":"Sharing and community curation of mass spectrometry data with Global Natural Products Social Molecular Networking","volume":"34","author":"Wang","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1038\/nmeth.3393","article-title":"MS-DIAL: Data-independent MS\/MS deconvolution for comprehensive metabolome analysis","volume":"12","author":"Tsugawa","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.jchromb.2016.09.034","article-title":"High throughput solid phase microextraction: A new alternative for analysis of cellular lipidome?","volume":"1043","author":"Birjandi","year":"2017","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1210\/clinem\/dgz240","article-title":"Metabolomics Signatures in Type 2 Diabetes: A Systematic Review and Integrative Analysis","volume":"105","author":"Sun","year":"2020","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1038\/oby.2009.510","article-title":"Increased levels of plasma acylcarnitines in obesity and type 2 diabetes and identification of a marker of glucolipotoxicity","volume":"18","author":"Mihalik","year":"2010","journal-title":"Obesity"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"8627","DOI":"10.1073\/pnas.1801609115","article-title":"Acetyl-l-carnitine deficiency in patients with major depressive disorder","volume":"115","author":"Nasca","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1111\/jdv.18631","article-title":"Mitochondrial dysfunction: The pathological link between psoriasis and insulin resistance?","volume":"37","year":"2023","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.lfs.2018.12.040","article-title":"The role of lysophosphatidic acid in the physiology and pathology of the skin","volume":"220","author":"Lei","year":"2019","journal-title":"Life Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/s41392-020-00379-1","article-title":"Lysophosphatidic acid mediates the pathogenesis of psoriasis by activating keratinocytes through LPAR5","volume":"6","author":"Lei","year":"2021","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-94-007-6331-9_1","article-title":"Sphingomyelin and its role in cellular signaling","volume":"991","author":"Chakraborty","year":"2013","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Burger, B., Sagiorato, R.N., Cavenaghi, I., and Rodrigues, H.G. (2023). Abnormalities of Sphingolipids Metabolic Pathways in the Pathogenesis of Psoriasis. Metabolites, 13.","DOI":"10.3390\/metabo13020291"}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/13\/8\/963\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:37:59Z","timestamp":1760128679000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/13\/8\/963"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,20]]},"references-count":51,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["metabo13080963"],"URL":"https:\/\/doi.org\/10.3390\/metabo13080963","relation":{},"ISSN":["2218-1989"],"issn-type":[{"value":"2218-1989","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,20]]}}}